Research Topics
Genomes and Genes | Chris J JanseSummaryAffiliation: Leiden University Medical Center Country: The Netherlands Publications
| Collaborators
|
Detail Information
Publications
Plasmodium Cysteine Repeat Modular Proteins 3 and 4 are essential for malaria parasite transmission from the mosquito to the hostBruno Douradinha
Malaria Unit, Instituto de Medicina Molecular, Faculdade de Medicina de Lisboa, Av Prof Egas Moniz, 1649 028 Lisbon, Portugal
Malar J 10:71. 2011..berghei sporozoites to the mosquito salivary gland and, hence, for transmission from the mosquito to the mouse...- Immunization with genetically attenuated P52-deficient Plasmodium berghei sporozoites induces a long-lasting effector memory CD8+ T cell response in the liverBruno Douradinha
Unidade de Malaria, Instituto de Medicina Molecular, Universidade de Lisboa, Av Professor Egas Moniz, Lisboa, 1649 028, Portugal
J Immune Based Ther Vaccines 9:6. 2011..abstract:.. - Selection by flow-sorting of genetically transformed, GFP-expressing blood stages of the rodent malaria parasite, Plasmodium bergheiChris J Janse
Nat Protoc 1:614-23. 2006..The application of flow-sorting to the selection of transformed parasites increases the possibilities of parasite mutagenesis, by effectively expanding the range of selectable markers... - High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium bergheiChris J Janse
Leiden University Medical Center, P O Box 9600, 2300 RC Leiden, The Netherlands
Nat Protoc 1:346-56. 2006..Genetic modification of P. berghei is widely used to investigate gene function in Plasmodium, and this protocol for high-efficiency transformation will enable the application of large-scale functional genomics approaches... - High efficiency transfection of Plasmodium berghei facilitates novel selection proceduresChris J Janse
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
Mol Biochem Parasitol 145:60-70. 2006..The combination of enhanced transfection efficiency and a versatile rodent model provides a basis for the further development of novel tools for high throughput genome manipulation... - A genotype and phenotype database of genetically modified malaria-parasitesChris J Janse
Leiden Malaria Research Group, Parasitology, Leiden University Medical Centre LUMC, Albinusdreef 2, 2333 ZA, Leiden, The Netherlands
Trends Parasitol 27:31-9. 2011..Access to mutant-parasite information, and gene function/ontology inferred from mutant phenotypes provides a timely resource aimed at enhancing research into Plasmodium gene function and (systems) biology... - Universal features of post-transcriptional gene regulation are critical for Plasmodium zygote developmentGunnar R Mair
Leiden Malaria Research Group, Department of Parasitology, Centre for Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
PLoS Pathog 6:e1000767. 2010..Therefore, translational repression may offer avenues as a target for the generation of transmission blocking strategies and contribute to limiting the spread of malaria... - Experimentally controlled downregulation of the histone chaperone FACT in Plasmodium berghei reveals that it is critical to male gamete fertilityEliane C Laurentino
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
Cell Microbiol 13:1956-74. 2011..Promoter swapping' is a powerful approach for the functional analysis of proteins in gametocytes (and beyond) that are essential during asexual blood-stage development... - Three members of the 6-cys protein family of Plasmodium play a role in gamete fertilityMelissa R van Dijk
Laboratory for Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
PLoS Pathog 6:e1000853. 2010.... - A conserved U-rich RNA region implicated in regulation of translation in Plasmodium female gametocytesJoanna A M Braks
Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre LUMC, Leiden, The Netherlands
Nucleic Acids Res 36:1176-86. 2008..These results demonstrate for the first time in a unicellular organism that the 5' or the 3'-UTR of TR-associated transcripts play an important and conserved role in mediating TR in female gametocytes... - Regulation of sexual development of Plasmodium by translational repressionGunnar R Mair
Department of Parasitology, Leiden University Medical Centre, 2333 ZA Leiden, Netherlands
Science 313:667-9. 2006..Genetic disruption of pbdozi inhibits the formation of the ribonucleoprotein complexes, and instead, at least 370 transcripts are diverted to a degradation pathway... - Reduced CD36-dependent tissue sequestration of Plasmodium-infected erythrocytes is detrimental to malaria parasite growth in vivoJannik Fonager
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, Netherlands
J Exp Med 209:93-107. 2012..These results reveal for the first time the importance of sequestration to a malaria infection, with implications for the development of strategies aimed at reducing pathology by inhibiting tissue sequestration... - Development of the piggyBac transposable system for Plasmodium berghei and its application for random mutagenesis in malaria parasitesJannik Fonager
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
BMC Genomics 12:155. 2011..P. berghei is the most frequently used malaria parasite model in gene function analysis since phenotype screens throughout the complete Plasmodium life cycle are possible both in vitro and in vivo... - Development and application of a positive-negative selectable marker system for use in reverse genetics in PlasmodiumJoanna A M Braks
Department of Parasitology, Centre of Infectious Diseases, Leiden University Medical Centre, The Netherlands
Nucleic Acids Res 34:e39. 2006..This is the first demonstration of the general use of yFCU in combination with a positive selectable marker in reverse genetics approaches and it should be possible to adapt its use to many other biological systems... - Functional characterization of the Plasmodium falciparum and P. berghei homologues of macrophage migration inhibitory factorKevin D Augustijn
Department of Parasitology, LUMC, Albinusdreef 2, Room P4 35, 2333 ZA Leiden, The Netherlands
Infect Immun 75:1116-28. 2007..Our results suggest that PMIF is produced by the parasite to influence host immune responses and the course of anemia upon infection... - A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycleBlandine Franke-Fayard
Department of Parasitology, Leiden University Medical Centre, Postbus 9600 RC, The Netherlands
Mol Biochem Parasitol 137:23-33. 2004..With the recent development of a second, independent selectable marker in P. berghei, PbGFPCON is a useful tool to investigate the effect of further genetic modifications on host-parasite interactions... - Plasmodium berghei alpha-tubulin II: a role in both male gamete formation and asexual blood stagesTaco W A Kooij
Department of Parasitology, Malaria Group, Leiden University Medical Centre, Albinusdreef 2, 2333 ZA Leiden, The Netherlands
Mol Biochem Parasitol 144:16-26. 2005..These results show that alpha-tubulin II not only plays an important role in the male gamete but is also expressed in and essential for asexual blood stage development... - Proteome analysis of separated male and female gametocytes reveals novel sex-specific Plasmodium biologyShahid M Khan
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
Cell 121:675-87. 2005..By identification of gender-specific protein kinases and phosphatases and using targeted gene disruption of two kinases, new sex-specific regulatory pathways were defined... - Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cellsMelissa R van Dijk
Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands
Proc Natl Acad Sci U S A 102:12194-9. 2005..Such knowledge of the biological characteristics of GAS and their evoked immune responses are essential for further investigation of the utility of an optimized GAS-based malaria vaccine... - Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestrationBlandine Franke-Fayard
Department of Parasitology, Leiden University Medical Center, 2300 RC, Leiden, The Netherlands
Proc Natl Acad Sci U S A 102:11468-73. 2005..Real-time in vivo imaging of parasitic processes may be used to evaluate the molecular basis of pathology and develop strategies to prevent pathology... - Malaria parasites lacking eef1a have a normal S/M phase yet grow more slowly due to a longer G1 phaseChris J Janse
Malaria Group, Department of Parasitology, Leiden University Medical Centre (LUMC, PO Box 9600, 2300 RC, Leiden, The Netherlands
Mol Microbiol 50:1539-51. 2003..Insight into protein synthesis and its influence on cell proliferation might be used to generate slow-growing (attenuated) parasites... - Sequestration and tissue accumulation of human malaria parasites: can we learn anything from rodent models of malaria?Blandine Franke-Fayard
Leiden Malaria Research Group, Department of Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
PLoS Pathog 6:e1001032. 2010..The role of sequestration is discussed in the context of disease as are the general (dis)similarities of P. berghei and P. falciparum sequestration... - The exoneme helps malaria parasites to break out of blood cellsChris J Janse
Department of Parasitology, Leiden University Medical Centre, Albinusdreef 2, 2333ZA Leiden, The Netherlands
Cell 131:1036-8. 2007..In this issue of Cell, Yeoh et al. (2007) discover a new organelle, the exoneme, that contains a protease SUB1, which helps the parasite to escape from old erythrocytes and invade new ones... - Assessing the adequacy of attenuation of genetically modified malaria parasite vaccine candidatesTakeshi Annoura
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Center, 2333 ZA Leiden, Netherlands
Vaccine 30:2662-70. 2012..Therefore, we propose a minimal set of screening criteria to assess adequacy of sporozoite attenuation necessary before advancing into further clinical development and studies in humans... - Real-time in vivo imaging of transgenic bioluminescent blood stages of rodent malaria parasites in miceBlandine Franke-Fayard
Malaria Research Group, Department of Parasitology, Leiden University Medical Center, PO Box 9600, 2300 RC Leiden, The Netherlands
Nat Protoc 1:476-85. 2006..Real-time in vivo imaging of infected erythrocytes will provide increased insights into the dynamics of sequestration and its role in pathology, and can be used to evaluate strategies that prevent sequestration... - Plasmodium post-genomics: better the bug you know?Taco W A Kooij
Malaria Research Group, Department of Parasitology, Centre for Infectious Diseases, Leiden University Medical Centre, The Netherlands
Nat Rev Microbiol 4:344-57. 2006..This review summarizes our current knowledge and how this has already been--and will continue to be--exploited in the search for vaccines and drugs against this most significant infectious disease of the tropics... - A novel 'gene insertion/marker out' (GIMO) method for transgene expression and gene complementation in rodent malaria parasitesJing Wen Lin
Leiden Malaria Research Group, Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
PLoS ONE 6:e29289. 2011..The implementation of GIMO-transfection procedures should greatly enhance Plasmodium reverse-genetic research... - Episomal transformation of Plasmodium bergheiChris J Janse
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
Methods Mol Med 72:305-15. 2002 - A Plasmodium whole-genome synteny map: indels and synteny breakpoints as foci for species-specific genesTaco W A Kooij
Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands
PLoS Pathog 1:e44. 2005..A majority of these genes are expressed in blood stages, suggesting that the vertebrate host exerts a greater selective pressure than the mosquito vector, resulting in the acquisition of diversity... - Topology and replication of a nuclear episomal plasmid in the rodent malaria Plasmodium bergheiDonald H Williamson
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK
Nucleic Acids Res 30:726-31. 2002..We propose a model in which continued rolling circle replication of the large circular concatamers and the recombinational activity of the tails of the rolling circles could lead to the formation of the large aggregates... - Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesisHiroshi Sakamoto
, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, France
Nucleic Acids Res 33:e174. 2005..The procedure opens the way to the systematic identification of essential genes in Plasmodium... - PTRAMP; a conserved Plasmodium thrombospondin-related apical merozoite proteinJoanne Thompson
Institute of Cell, Animal and Population Biology, Ashworth Laboratories, The King s Buildings, Edinburgh EH9 3JT, UK
Mol Biochem Parasitol 134:225-32. 2004..PTRAMP, therefore, has all the features to merit further evaluation as a malaria vaccine candidate... - Real-time, in vivo analysis of malaria ookinete locomotion and mosquito midgut invasionDina Vlachou
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Cell Microbiol 6:671-85. 2004..Similar observations were made in both A. gambiae and A. stephensi, demonstrating the conservation of P. berghei interactions with these vectors... - Set regulation in asexual and sexual Plasmodium parasites reveals a novel mechanism of stage-specific expressionTomasino Pace
Dipartimento di Malattie Infettive Parassitarie ed Immunomediate, Istituto Superiore di Sanit, Viale Regina Elena 299, 00161 Roma, Italy
Mol Microbiol 60:870-82. 2006..In male gametocytes an overexpression of SET might contribute to a prompt entry and execution of S/M phases within mosquito vector... - A comprehensive survey of the Plasmodium life cycle by genomic, transcriptomic, and proteomic analysesNeil Hall
Pathogen Sequencing Unit, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge CB10 1SA, UK
Science 307:82-6. 2005..We observed posttranscriptional gene silencing through translational repression of messenger RNA during sexual development, and a 47-base 3' untranslated region motif is implicated in this process... - A role for natural regulatory T cells in the pathogenesis of experimental cerebral malariaFiona H Amante
Queensland Institute of Medical Research, Herston, QLD 4006, Australia
Am J Pathol 171:548-59. 2007.... - The Plasmodium TRAP/MIC2 family member, TRAP-Like Protein (TLP), is involved in tissue traversal by sporozoitesCristina K Moreira
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY 10021, USA
Cell Microbiol 10:1505-16. 2008..These results indicate a role of TLP in cell traversal by sporozoites... - Plasmodium cysteine repeat modular proteins 1-4: complex proteins with roles throughout the malaria parasite life cycleJoanne Thompson
Institute of Immunology and Infection Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JT, UK
Cell Microbiol 9:1466-80. 2007..The unprecedented expression pattern and structural features of the PCRMPs thus suggest a variety of roles mediating host-parasite interactions throughout the parasite life cycle... - The use of transgenic Plasmodium berghei expressing the Plasmodium vivax antigen P25 to determine the transmission-blocking activity of sera from malaria vaccine trialsSouraya Ramjanee
Division of Cell and Molecular Biology, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK
Vaccine 25:886-94. 2007..To this end the cloned lines have been deposited with, and are freely available from, MR4... - Genome sequence and comparative analysis of the model rodent malaria parasite Plasmodium yoelii yoeliiJane M Carlton
The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
Nature 419:512-9. 2002..This is the first genome sequence of a model eukaryotic parasite, and it provides insight into the use of such systems in the modelling of Plasmodium biology and disease... - Species-specific inhibition of cerebral malaria in mice coinfected with Plasmodium sppTatiana Voza
, , CNRS IFR 101, CP52, 61 rue Buffon, 75231 Paris Cedex 05, France
Infect Immun 73:4777-86. 2005..These observations establish the concept of mixed Plasmodium species infections as potential modulators of pathology and open novel avenues to investigate mechanisms implicated in the pathogenesis of malaria... - Pfs47, paralog of the male fertility factor Pfs48/45, is a female specific surface protein in Plasmodium falciparumBen C L van Schaijk
Department of Medical Microbiology, Radboud University Nijmegen Medical Center, 268, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands
Mol Biochem Parasitol 149:216-22. 2006..These results show redundancy in protein function for Pfs47 and reduce the support for candidacy of Pfs47 as a transmission-blocking vaccine target... - Gene targeting demonstrates that the Plasmodium berghei subtilisin PbSUB2 is essential for red cell invasion and reveals spontaneous genetic recombination eventsPierrick Uzureau
, Institut Pasteur/CNRS URA 2581, 25 rue du Dr Roux, 75015 Paris, France
Cell Microbiol 6:65-78. 2004..berghei... - Complement-like protein TEP1 is a determinant of vectorial capacity in the malaria vector Anopheles gambiaeStephanie Blandin
European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Cell 116:661-70. 2004..Further elucidation of the molecular mechanisms of TEP1-mediated parasite killing will be of great importance for our understanding of the principles of vectorial capacity in insects... - Plasmodium lipid rafts contain proteins implicated in vesicular trafficking and signalling as well as members of the PIR superfamily, potentially implicated in host immune system interactionsFrancesco Di Girolamo
Dipartimento di Malattie Infettive Parassitarie ed Immunomediate, Istituto Superiore di Sanita, Roma, Italy
Proteomics 8:2500-13. 2008..Our results indicated that lipid rafts in Plasmodium-infected erythrocytes might constitute a route to sort and fold parasite proteins directed to various host cell compartments including the cell surface... - Genetically attenuated P36p-deficient Plasmodium berghei sporozoites confer long-lasting and partial cross-species protectionBruno Douradinha
Unidade Malaria, Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, 1649 028, Lisboa, Portugal
Int J Parasitol 37:1511-9. 2007....