Research Topics
| Pavlina KonstantinovaSummaryAffiliation: Academic Medical Center Country: The Netherlands Publications
| Collaborators
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Detail Information
Publications
Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genomePavlina Konstantinova
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands
Retrovirology 4:15. 2007..In order to solve this problem, we constructed an HIV-1 variant with a 300 bp long hairpin structure in the 3' part of the genome corresponding to the Nef gene (HIV-lhNef)...
Silencing of HIV-1 with RNA interference: a multiple shRNA approachOlivier Ter Brake
Department of Human Retrovirology, University of Amsterdam, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
Mol Ther 14:883-92. 2006..These results confirm that RNAi has great potential as an antiviral gene therapy approach and support our efforts to develop this strategy for treatment of HIV-1-infected individuals...
RNAi-mediated inhibition of HIV-1 by targeting partially complementary viral sequencesYing Poi Liu
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, The Netherlands
Nucleic Acids Res 37:6194-204. 2009..These data suggest that targeting imperfect target sites by antiviral miRNAs or shRNAs provides an alternative RNAi approach for inhibition of pathogenic viruses...
Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistronYing Poi Liu
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Nucleic Acids Res 36:2811-24. 2008..These combined results indicate that a multiplex miRNA strategy may be a promising therapeutic approach to attack escape-prone viral pathogens...
Titers of lentiviral vectors encoding shRNAs and miRNAs are reduced by different mechanisms that require distinct repair strategiesYing Poi Liu
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
RNA 16:1328-39. 2010..These results show that not all sequences are compatible with the lentiviral vector system and that care should be taken in the design of lentiviral vectors encoding RNAi inducers...
Optimization of shRNA inhibitors by variation of the terminal loop sequenceNick C T Schopman
Laboratory of Experimental Virology, Department of Medical Microbiology, Center for Infection and Immunity Amsterdam CINIMA, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, The Netherlands
Antiviral Res 86:204-11. 2010..The optimized shRNA loop sequence is generally applicable for RNAi knockdown studies, and will allow us to develop a more potent gene therapy against HIV-1...
In vivo knock-down of multidrug resistance transporters ABCC1 and ABCC2 by AAV-delivered shRNAs and by artificial miRNAsFlorie Borel
Department of Research and Development, Amsterdam Molecular Therapeutics, Amsterdam, The Netherlands
J RNAi Gene Silencing 7:434-42. 2011..These results demonstrate the feasibility of knocking down Abbc2 via AAV-delivered shRNAs to the liver, and encourage the use of miRNA in further therapeutics development...
Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1Pavlina Konstantinova
Department of Human Retrovirology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands
Nucleic Acids Res 34:2206-18. 2006..The transient role of the cellular tRNA molecule as enhancer of retroviral recombination is illustrated by the eventual removal of inserted tRNA sequences by a subsequent recombination/deletion event...
Adenosine triphosphate-binding cassette transporter genes up-regulation in untreated hepatocellular carcinoma is mediated by cellular microRNAsFlorie Borel
Department of Research and Development, Amsterdam Molecular Therapeutics, Amsterdam, The Netherlands
Hepatology 55:821-32. 2012..Up-regulation of five ABC genes appears to be mediated by 13 cellular miRNAs in HCC patient samples. miRNA-based gene therapy may be a novel and promising way to affect the ABC profile and overcome clinical multidrug resistance...
Apolipoprotein B knockdown by AAV-delivered shRNA lowers plasma cholesterol in miceAnnemart Koornneef
Department of Research and Development, Amsterdam Molecular Therapeutics, Amsterdam, The Netherlands
Mol Ther 19:731-40. 2011..These results demonstrate a robust dose-dependent knockdown of ApoB by AAV-delivered shRNA in murine liver, thus providing an excellent candidate for development of RNAi-based gene therapy for the treatment of hypercholesterolemia...
