Research Topics
| T W FlegelSummaryAffiliation: Mahidol University Country: Thailand Publications
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Detail Information
Publications
Seasonal variation in white spot syndrome virus-positive samples in broodstock and post-larvae of Penaeus monodon in ThailandBoonsirm Withyachumnarnkul
Centex Shrimp, Chalerm Prakiat Building, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Dis Aquat Organ 53:167-71. 2003....- Complete Genome Sequence of Virulence-Enhancing Siphophage VHS1 from Vibrio harveyiKrit Khemayan
Address correspondence to Timothy W Flegel
Appl Environ Microbiol 78:2790-6. 2012..The last protein was identified as a component of the species-specific toxin mixture described above as being associated with agglutination of hemocytes from P. monodon... - False rumours of disease outbreaks caused by infectious myonecrosis virus (IMNV) in the whiteleg shrimp in AsiaSaengchan Senapin
National Center for Genetic Engineering and Biotechnology BIOTEC, National Science and Technology Development Agency NSTDA, Pathumthani, 12120, Thailand
J Negat Results Biomed 10:10. 2011..In 2006 the first disease outbreaks caused by IMNV in Asia occurred in Indonesia. Since then rumours have periodically circulated about IMNV disease outbreaks in other Asian countries. Our findings indicate that these are false rumours... - A novel integrase-containing element may interact with Laem-Singh virus (LSNV) to cause slow growth in giant tiger shrimpWattana Panphut
Centex Shrimp, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
BMC Vet Res 7:18. 2011.... - Mud crab susceptibility to disease from white spot syndrome virus is species-dependentNaraporn Somboonna
Shrimp Virus Interaction Laboratory, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Klong Luang, Pathumthani 12120, Thailand
BMC Res Notes 3:315. 2010..We compared single-dose and serial-dose WSSV challenges on S. olivacea and S. paramamosain... - Persistent, triple-virus co-infections in mosquito cellsNipaporn Kanthong
Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
BMC Microbiol 10:14. 2010..By addition of Japanese encephalitis virus (JE), we tested our hypothesis that stable, persistent, triple-virus co-infections could be obtained by the same process... - Hepatopancreatic nuclease of black tiger shrimp Penaeus monodon unlikely to be involved in viral triggered apoptosisSudkhate Molthathong
Center of Excellence for Shrimp Molecular Biology and Biotechnology Centex Shrimp, Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Fish Shellfish Immunol 22:617-27. 2007..The results suggested that PMN is probably a digestive enzyme that is unlikely to be involved in hallmark DNA digestion associated with apoptosis... - Hypothesis for heritable, anti-viral immunity in crustaceans and insectsTimothy W Flegel
Center of Excellence for Shrimp Molecular Biology and Biotechnology Centex Shrimp, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Biol Direct 4:32. 2009..However, recent developments suggest that specific responses may be based on viral sequences inserted into the host genome... - Shrimp molecular responses to viral pathogensT W Flegel
National Science and Technology Development Agency NSTDA, Klong Luang, Pathumthani 12120, Thailand
Mar Biotechnol (NY) 13:587-607. 2011..e., the quasi immune response, viral co-infections, viral sequences in the shrimp genome and persistent viral infections) for which little or no molecular information is currently available, but is much needed... - Response to Dengue virus infections altered by cytokine-like substances from mosquito cell culturesNipaporn Kanthong
Department of Biotechnology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok, Thailand
BMC Microbiol 10:290. 2010..As a result, we accidentally discovered two new cytokine-like substances in 5 kDa extracts from supernatant solutions of acutely and persistently infected mosquito cells... - Update on viral accommodation, a model for host-viral interaction in shrimp and other arthropodsT W Flegel
Center of Excellence for Shrimp Molecular Biology and Biotechnology Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Dev Comp Immunol 31:217-31. 2007..e., accommodated viral infections) may be a key process that has evolved from host viral interaction in the arthropod line... - Different reactions obtained using the same DNA detection reagents for Thai and Korean hepatopancreatic parvovirus of penaeid shrimpJ Phromjai
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
Dis Aquat Organ 46:153-8. 2001.... - Shrimp Taura syndrome virus detection by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstickWansika Kiatpathomchai
National Center for Genetic Engineering and Biotechnology BIOTEC, National Science and Technology Development Agency, Pathumthani 12120, Thailand
J Virol Methods 153:214-7. 2008..In addition to reduced assay time when compared to electrophoresis, combination of RT-LAMP with LFD confirms amplicon identity by hybridization and eliminates the need to handle carcinogenic ethidium bromide... - Impact of yellow head virus outbreaks in the whiteleg shrimp, Penaeus vannamei (Boone), in ThailandS Senapin
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani, Thailand
J Fish Dis 33:421-30. 2010..Thus, it is possible that they originated from an unknown, natural reservoir... - Identification and characterization of Alix/AIP1 interacting proteins from the black tiger shrimp, Penaeus monodonP Sangsuriya
Centex Shrimp, Faculty of Science, Mahidol University, Bangkok, Thailand
J Fish Dis 33:571-81. 2010..Thus, the results suggested that Alix and its interacting partners did not play a direct role related to shrimp death... - Rapid and sensitive detection of Taura syndrome virus by reverse transcription loop-mediated isothermal amplificationWansika Kiatpathomchai
National Center for Genetic Engineering and Biotechnology BIOTEC, National Science and Technology Development Agency, Pathumthani 12120, Thailand
J Virol Methods 146:125-8. 2007..The detection of TSV using RT-LAMP was 10 times more sensitive than the RT-PCR but less sensitive than nested RT-PCR. However this system was more convenient, rapid, and does not require sophisticated PCR machine... - Target for standard Thai PCR assay identical in 12 white spot syndrome virus (WSSV) types that differ in DNA multiple repeat lengthWansika Kiatpathomchai
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand
J Virol Methods 130:79-82. 2005..If so, false negative results could be reduced by the use of nested PCR assays with larger PL sample sizes. ... - Shrimp hepatopancreatic parvovirus detection by combining loop-mediated isothermal amplification with a lateral flow dipstickTongchai Nimitphak
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
J Virol Methods 154:56-60. 2008.... - Down-regulation of defender against apoptotic death (DAD1) after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodonSudkhate Molthathong
Centex Shrimp, Faculty of Science, Mahidol University, Bangkok, Thailand
Fish Shellfish Immunol 24:173-9. 2008..Thus, firm conclusions regarding the role of DAD1 must await the description and characterization of other proteins... - Taura syndrome virus (TSV) in Thailand and its relationship to TSV in China and the AmericasLinda Nielsen
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Dis Aquat Organ 63:101-6. 2005..vannamei and P. japonicus from China. Comparison revealed that the Thai, Burmese and Chinese TSV types formed a clade distinct from a clade of TSV types from the Americas... - Comparison of penaeid shrimp and insect parvoviruses suggests that viral transfers may occur between two distantly related arthropod groupsSongsak Roekring
Department of Biotechnology, Faculty of Science, Centex Shrimp, Chalerm Prakiat Bldg, Mahidol University, Bangkok, Thailand
Virus Res 87:79-87. 2002..The four shrimp parvoviruses fell into two different clades that grouped with different insect parvoviruses... - Evidence for apoptosis correlated with mortality in the giant black tiger shrimp Penaeus monodon infected with yellow head virusKornnika Khanobdee
Centex Shrimp, Mahidol University, Bangkok, Thailand
Dis Aquat Organ 48:79-90. 2002..This probably resulted in the gross signs of lethargy and weakness seen, and it is reasonable to suggest that further, progressive deterioration could have led to the collapse of vital functions followed by death... - Knocking down a Taura syndrome virus (TSV) binding protein Lamr is lethal for the whiteleg shrimp Penaeus vannameiSaengchan Senapin
Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Rama VI Rd, Bangkok 10400, Thailand
Fish Shellfish Immunol 29:422-9. 2010..vannamei led to shrimp mortality and indicated an essential function of Lamr for shrimp viability. A negative consequence was that the effect of Lamr knockdown on shrimp infection by Taura syndrome virus could not be assessed... - Up-regulation of ribophorin I after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodonSudkhate Molthathong
Centex Shrimp, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Fish Shellfish Immunol 25:40-6. 2008..However, unlike DAD1 that was down-regulated after YHV challenge, ribophorin I expression was up-regulated and remained high until the moribund stage... - Detection of hepatopancreatic parvovirus (HPV) infection in Penaeus monodon using PCR-ELISAW Sukhumsirichart
Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Bangkok, 10110, Thailand
Mol Cell Probes 16:409-13. 2002..Therefore, this sensitive and specific method is promisingly useful for early detection of HPV infection in broodstock, carriers and for ex situ application where large numbers of samples can be analyzed simultaneously... - A new RNA virus found in black tiger shrimp Penaeus monodon from ThailandKallaya Sritunyalucksana
Centex Shrimp, Faculty of Science, Mahidol University and National Center for Genetic Engineering and Biotechnology, BIOTEC, National Science and Technology Development Agency, NSTDA, Klong Luang, Pathumthani 12120, Thailand
Virus Res 118:31-8. 2006..Although not directly associated with MSGS in Penaeus monodon, the presence or effect of this virus with other crustacean species is presently unknown... - Rapid and sensitive detection of white spot syndrome virus by loop-mediated isothermal amplification combined with a lateral flow dipstickWansadaj Jaroenram
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Ratchathewi, Bangkok 10400, Thailand
Mol Cell Probes 23:65-70. 2009.... - Characterization and tissue expression of apoptosis-related ALG-2 interacting protein Alix/AIP1 from the black tiger shrimp Penaeus monodonPakkakul Sangsuriya
Center of Excellence for Shrimp Molecular Biology and Biotechnology, Mahidol University, Chalermprakiat Building, Bangkok 10400, Thailand
Fish Shellfish Immunol 23:485-92. 2007 - Low viral loads and lymphoid organ spheroids are associated with yellow head virus (YHV) tolerance in whiteleg shrimp Penaeus vannameiGun Anantasomboon
Department of Anatomy, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
Dev Comp Immunol 32:613-26. 2008..Thus, YHV-1 tolerance was not associated with absence of gp116 as previously reported for palaemonid shrimp. Instead, it was associated with the presence of YHV-positive LOS and a relatively low viral load... - Bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimpKallaya Sritunyalucksana
National Center for Genetic Engineering and Biotechnology (BIOTEC) and Centex Shrimp, Bangkok 10400, Thailand
Dis Aquat Organ 63:89-94. 2005..These 2 methods provide a simple and rapid comparison of shrimp groups for differences in anti-bacterial defense capacity... - Characterization of a shrimp serine protease homolog, a binding protein of yellow head virusThanawat Sriphaijit
Department of Biotechnology, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
Dev Comp Immunol 31:1145-58. 2007..Immunohistochemistry using a polyclonal antibody raised against shrimp protein SPH516-C heterologously expressed in Escherichia coli revealed that SPH516 was present almost exclusively in the shrimp hemolymph... - Detection of hepatopancreatic parvovirus in Thai shrimp Penaeus monodon by in situ hybridization, dot blot hybridization and PCR amplificationJurairat Phromjai
Centex Shrimp, Chalerm Prakiat Building, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
Dis Aquat Organ 51:227-32. 2002..1 pg HPVmon DNA by the PCR reaction. By dot blot hybridization, a visible signal was obtained with purified HPVmon DNA at 0.01 pg, but detection in spiked feces and postlarval samples was only 1 and 0.1 pg, respectively... - Unstable lysogeny and pseudolysogeny in Vibrio harveyi siphovirus-like phage 1Krit Khemayan
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Appl Environ Microbiol 72:1355-63. 2006..Such clones may be called pseudolysogens (i.e., false lysogens), since they have undergone transient lysogeny and have retained some resistance to full lytic phage development, despite the loss of viable or detectable prophage... - Detection and differentiation of yellow head complex viruses using monoclonal antibodiesChumporn Soowannayan
National Center for Genetic Engineering and Biotechnology, and Centex Shrimp Chalermprakiat Building, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Dis Aquat Organ 57:193-200. 2003..These data show that the 3 YHV MAbs could be used in diagnostic situations to differentiate some viruses in the yellow head virus complex... - Parvo-like virus in the hepatopancreas of freshwater prawns Macrobrachium rosenbergii cultivated in ThailandWarachin Gangnonngiw
Centex Shrimp, Chalerm Prakiat Building, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
Dis Aquat Organ 85:167-73. 2009..These results suggested that the inclusions contained a parvo-like virus, not previously reported from M. rosenbergii in Thailand... - Analysis of differently expressed proteins and transcripts in gills of Penaeus vannamei after yellow head virus infectionTriwit Rattanarojpong
Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
Proteomics 7:3809-14. 2007..The present results are consistent with previously published work and suggest that a decrease in SCP expression may play an important role in the shrimp response to viral infections in general... - Detection of shrimp infectious myonecrosis virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstickTeeranart Puthawibool
Centex Shrimp, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand
J Virol Methods 156:27-31. 2009..monodon (PmDNV), white spot syndrome virus (WSSV), yellow head virus (YHV), Taura syndrome virus (TSV), Macrobrachium rosenbergii nodavirus (MrNV) and gill associated virus (GAV)... - Partial characterization of a novel bacteriophage of Vibrio harveyi isolated from shrimp culture ponds in ThailandTirasak Pasharawipas
Department of Microbiology, Faculty of Science, Rangsit University, Pathumthani 12000, Thailand
Virus Res 114:63-9. 2005..Approximately 20 kb of the genome has been sequenced and deposited at GenBank but it mostly showed no significant homology with existing sequences in the database... - PmRab7 is a VP28-binding protein involved in white spot syndrome virus infection in shrimpKallaya Sritunyalucksana
National Center for Genetic Engineering and Biotechnology (BIOTEC, National Science and Technology Development Agency, Pathumthani 12120, Thailand
J Virol 80:10734-42. 2006..We conclude that PmRab7 is involved in WSSV infection in shrimp. This is the first study to identify a shrimp protein that binds directly to a major viral envelope protein of WSSV... - Markedly reduced severity of Dengue virus infection in mosquito cell cultures persistently infected with Aedes albopictus densovirus (AalDNV)Peangpim Burivong
Division of Medical Molecular Biology, Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Virology 329:261-9. 2004..The phenomena described raise questions regarding the potential for persistent infections by unknown viruses to confound experimental results with insect cell lines...