Research Topics
| Suh Chin WuSummaryAffiliation: National Tsing Hua University Country: Taiwan Publications
| Collaborators
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Detail Information
Publications
Stationary and microcarrier cell culture processes for propagating Japanese encephalitis virusSuh-Chin Wu
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 30013
Biotechnol Prog 18:124-8. 2002..Thus, the stationary culture is the most efficient system for JEV production from cultured Vero cells...- High immunogenic enterovirus 71 strain and its production using serum-free microcarrier Vero cell cultureChia Chyi Liu
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
Vaccine 25:19-24. 2007..Therefore, serum-free microcarrier culture is a valuable process for developing inactivated EV71 vaccines... - Japanese encephalitis virus antigenic variants with characteristic differences in neutralization resistance and mouse virulenceS C Wu
National Institute of Preventive Medicine, Taipei, Taiwan
Virus Res 51:173-81. 1997..Our results indicated the neutralizing epitope of Japanese encephalitis virus did not overlap with virus virulence determinant... - The domain III fragment of Japanese encephalitis virus envelope protein: mouse immunogenicity and liposome adjuvanticitySuh Chin Wu
Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan
Vaccine 21:2516-22. 2003..These studies can provide useful information for further developing the domain III recombinant protein vaccine against JEV... - Construction and characterization of a Fab recombinant protein for Japanese encephalitis virus neutralizationSuh Chin Wu
Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan
Vaccine 23:163-71. 2004..The information gained from this study may benefit the design of vaccines and therapeutic antibodies against JEV infection... - Optimization of microcarrier cell culture process for the inactivated enterovirus type 71 vaccine developmentSuh Chin Wu
Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan
Vaccine 22:3858-64. 2004..These results constitute valuable information on the development of a large-scale microcarrier cell culture process for producing inactivated EV71 vaccine... - Novel cell adhesive glycosaminoglycan-binding proteins of Japanese encephalitis virusSuh Chin Wu
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan, Republic of China
Biomacromolecules 5:2160-4. 2004..The present studies demonstrate the recombinant GAG-binding proteins of JEV stimulate cell adhesive and proliferation with a potential for applications in tissue engineering... - Short hairpin RNA targeted to dihydrofolate reductase enhances the immunoglobulin G expression in gene-amplified stable Chinese hamster ovary cellsSuh Chin Wu
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
Vaccine 26:4969-74. 2008..Utilizing the dhfr-targeted shRNA vector may provide an alternative way to maneuver CHO cell factories for IgG production in cultures... - RNA interference technology to improve recombinant protein production in Chinese hamster ovary cellsSuh Chin Wu
Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Biotechnol Adv 27:417-22. 2009.... - Production of retrovirus and adenovirus vectors for gene therapy: a comparative study using microcarrier and stationary cell cultureSuh Chin Wu
Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Biotechnol Prog 18:617-22. 2002..These findings can provide further insight into the feasibility of applying microcarrier cell culture technology to produce gene-therapy virus vectors... - Characterization of the GXXXG motif in the first transmembrane segment of Japanese encephalitis virus precursor membrane (prM) proteinYing Ju Lin
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
J Biomed Sci 17:39. 2010..The prM transmembrane domains of JEV may play importation roles in prM-E heterodimerization and viral particle assembly... - Complete nucleotide sequence and cell-line multiplication pattern of the attenuated variant CH2195LA of Japanese encephalitis virusS C Wu
Department of Life Science, National Tsing Hua University, 30043, Hsinchu, Taiwan, ROC
Virus Res 73:91-102. 2001..These results revealed that the attenuated variant CH2195LA was unique to culture in Vero cells... - Antigenic characterization of nine wild-type Taiwanese isolates of Japanese encephalitis virus as compared with two vaccine strainsS C Wu
Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
Virus Res 55:83-91. 1998..Phylogenetic tree analysis indicates these two wild-type Taiwanese isolates are more distant from the two vaccine strains... - Increase of primary HIV-1 production in human peripheral blood mononuclear cells by intermittent medium replenishmentS C Wu
National Center for Biotechnology Information, National Library of Medicine, Bethesda, Maryland 20894, USA
Biotechnol Prog 14:540-2. 1998..The optimal rate for intermittent medium replenishment to achieve the lowest viral particle-to-infectious unit ratio is around 0.25 volume/volume/day... - Phenotypic and genotypic characterization of the neurovirulence and neuroinvasiveness of a large-plaque attenuated Japanese encephalitis virus isolateSuh Chin Wu
Department of Life Science, Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC
Microbes Infect 5:475-80. 2003..Elucidating the molecular basis of virulence of flaviviruses can provide valuable information for live-attenuated vaccine development... - Enhanced antibody affinity to Japanese encephalitis virus E protein by phage displayWilly W L Hong
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Biochem Biophys Res Commun 356:124-8. 2007..The information gained from this study may benefit the design of vaccines and therapeutic antibodies against Japanese encephalitis virus infection... - A novel RNA silencing vector to improve antigen expression and stability in Chinese hamster ovary cellsWilly W L Hong
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Vaccine 25:4103-11. 2007..Utilization of the silencing vector designed in this study can improve antigen expression through dhfr-directed gene amplification in other dhfr-competent cell lines for vaccine development... - Histidine at residue 99 and the transmembrane region of the precursor membrane prM protein are important for the prM-E heterodimeric complex formation of Japanese encephalitis virusYing Ju Lin
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan, Republic of China
J Virol 79:8535-44. 2005.... - Neutralizing peptide ligands selected from phage-displayed libraries mimic the conformational epitope on domain III of the Japanese encephalitis virus envelope proteinS C Wu
Department of Life Science, National Tsing Hua University, 30013, Taiwan, Hsinchu, PR China
Virus Res 76:59-69. 2001..Our results showed that three residues forming the novel cis-proline turn structure were all important in eliciting JEV-specific neutralizing antibodies in mice... - Fabrication of influenza virus-like particles using M2 fusion proteins for imaging single viruses and designing vaccinesHung Ju Wei
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Vaccine 29:7163-72. 2011..The results can provide useful information for imaging single viruses and designing novel vaccines against influenza virus infection... - High genetic stability of dengue virus propagated in MRC-5 cells as compared to the virus propagated in vero cellsChia Chyi Liu
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
PLoS ONE 3:e1810. 2008..Serum-free microcarrier cultures of MRC-5 cells offer a potentially valuable system for the large-scale production of live-attenuated DEN vaccines... - Generating stable Chinese hamster ovary cell clones to produce a truncated SARS-CoV spike protein for vaccine developmentShih Chang Lin
Institute of Biotechnology, Dept of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Biotechnol Prog 26:1733-40. 2010..The S(TR2) glycoform did not change between suspension and serum-free cultures, suggesting that the stable and amplified cell clones analyzed in this study have potential for producing homologous S(TR2) on a large scale... - An intron promotes the anti-bcr-abl activities of a retrovirally expressed ribozyme in chronic myeloid leukemia cellsJin Hwang Liu
Division of Medical Oncology, Taipei Veterans General Hospital and National Yang Ming University, Taipei, Taiwan, ROC
Biochem Biophys Res Commun 318:764-72. 2004..Noticeably, the improvement is contributed in part by subcellular co-localization... - Identification of a heparin binding peptide from the Japanese encephalitis virus envelope proteinHeng Li Chen
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 300, Taiwan, China
Biopolymers 94:331-8. 2010..Our results provide a basis for further understanding the interactions of flaviviruses and glycosaminoglycans on the host cell surfaces... - Dengue type 4 live-attenuated vaccine viruses passaged in vero cells affect genetic stability and dengue-induced hemorrhaging in miceHsiang Chi Lee
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
PLoS ONE 6:e25800. 2011..Taken together, the genetic stability, virus yield, and DEN-induced hemorrhaging all require further investigation in the context of live-attenuated DEN vaccine development... - Dendritic cell activation by recombinant hemagglutinin proteins of H1N1 and H5N1 influenza A virusesWen Chun Liu
Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan
J Virol 84:12011-7. 2010..The stimulation of mDCs by HA proteins of H1N1 and H5N1 was completely MyD88 dependent. These findings may provide useful information for the development of more-effective influenza vaccines... - A novel E1B-55kD-deleted oncolytic adenovirus carrying mutant KRAS-regulated hdm2 transgene exerts specific antitumor efficacy on colorectal cancer cellsChin Cheng Liu
Institute of Biochemistry and Molecular Biology, Taipei Veterans General Hospital, School of Medicine, National Yang Ming University, Taipei, Taiwan, Republic of China
Mol Cancer Ther 9:450-60. 2010..Ad-KRhdm2 has shown its therapeutic potential in KRAS(aMut) cancer cells and warrants further clinical trials... - Recombinant trimeric HA protein immunogenicity of H5N1 avian influenza viruses and their combined use with inactivated or adenovirus vaccinesShih Chang Lin
Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan
PLoS ONE 6:e20052. 2011..The highly pathogenic avian influenza (HPAI) H5N1 virus continues to cause disease in poultry and humans. The hemagglutinin (HA) envelope protein is the primary target for subunit vaccine development... - Isolation of therapeutically functional mouse bone marrow mesenchymal stem cells within 3 h by an effective single-step plastic-adherent methodF S H Hsiao
Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan
Cell Prolif 43:235-48. 2010..Herein, we provide a single-step procedure to rapidly segregate mMSCs from HC contaminants using transient lower-density plastic adherence (tLDA)... - A prospective study of p53 expression and its correlation with clinical response of radiotherapy in nasopharyngeal carcinomaK Y Ho
Department of Otorhinolaryngology, Kaohsiung Medical University Hospital, Kaohsiung City, Taiwan, Republic of China
Laryngoscope 111:131-6. 2001..05, chi2 test). CONCLUSIONS: We conclude that there is no statistically significant correlation in locoregional response of primary tumor between p53 overexpression and radiotherapy in patients with NPC (P > .05, Fisher exact test)... - Age, period, and cohort effects on the attitude toward supporting parents in TaiwanH C Hsu
Institute of Public Health, National Taiwan University, Taipei, Republic of China
Gerontologist 41:742-50. 2001..Using the perspectives of age, period, and cohort (APC) effects, this study explored the changes in attitudes toward supporting parents in Taiwan... - Identification of physical parameters controlling the dominance of algal species in a subtropical reservoirY C Chien
Graduate Institute of Environmental Engineering, National Taiwan University, 71 Chou Shan Road, Taipei 10673, Taiwan, ROC
Water Sci Technol 60:1779-86. 2009..According to our preliminary findings, the influence of physical factors, varying seasonally, outweighs the influence of nutrients on the algal species composition in Feitsui Reservoir in Taiwan... - Immunohistochemical diagnosis of mouse hepatitis virus and mycoplasma pulmonis infection with murine antiserumC T Liang
National Laboratory Animal Center, National Applied Research Laboratories, Nan-Kang, Taipei 115, Taiwan, ROC
J Comp Pathol 131:214-20. 2004..M. pulmonis antigen was demonstrated on the luminal surface of bronchiolar epithelial cells. This method may prove useful in diagnosis when commercial antisera are unavailable or when immunosuppression prevents serological diagnosis... - Relationship between fetal nuchal translucency and crown-rump length in an Asian populationH J Jou
Department of Obstetrics and Gynecology, Taiwan Adventist Hospital, Taipei, Taiwan, ROC
Ultrasound Obstet Gynecol 17:111-4. 2001.... - Leukopenia and thrombocytopenia in hemodialysis patients with hepatitis B or C virus infection and non-hemodialysis patients with hepatitis cirrhosisY Y Ng
Department of Medicine, Taipei Veterans General Hospital, Taiwan, Republic of China
Clin Nephrol 57:289-95. 2002..To investigate the relation of leukopenia and thrombocytopenia in hemodialysis (HD) patients with hepatitis C virus (HCV) infection... - A functional epitope determinant on domain III of the Japanese encephalitis virus envelope protein interacted with neutralizing-antibody combining sitesCheng-Wen Lin
Institute of Biotechnology and Department of Life Science, National Tsing-Hua University, Hsinchu 30013, Taiwan
J Virol 77:2600-6. 2003..This study elucidates the detailed molecular structures of the neutralizing epitope determinants on JEV domain III, which can provide useful information for designing new vaccines... - Mosquito and mammalian cells grown on microcarriers for four-serotype dengue virus production: variations in virus titer, plaque morphology, and replication rateChia-Chyi Liu
Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Biotechnol Bioeng 85:482-8. 2004..Interestingly, some selected variants of DEN virus may outgrow in Vero cells when using a T-flask culture. These results may provide useful information for DEN vaccine development... - Identification of mimotopes of the Japanese encephalitis virus envelope protein using phage-displayed combinatorial peptide libraryCheng Wen Lin
Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan
J Mol Microbiol Biotechnol 8:34-42. 2004..The results of our study could provide useful information on the development of effective mimotope-based vaccines and diagnostic kits for the JEV infection... - Influence of intron and exon splicing enhancers on mammalian cell expression of a truncated spike protein of SARS-CoV and its implication for subunit vaccine developmentChia-Yin Chang
Institute of Biotechnology, Department of Life Science, National Tsing Hua University, Hsinchu 30013, Taiwan
Vaccine 24:1132-41. 2006..Results of the present study can provide an optimal strategy to enhance SARS-CoV S protein expression in mammalian cells and may contribute to the development of SARS-CoV subunit vaccine... - Artificial extracellular matrix proteins contain heparin-binding and RGD-containing domains to improve osteoblast-like cell attachment and growthSuh-Chin Wu
Institute of Biotechnology, Department of Life Science, National Tsing-Hua University, Hsinchu, Taiwan
J Biomed Mater Res A 79:557-65. 2006..This study is the first report of the construction of aECM proteins that contain both heparin-binding and RGD-containing domains used in osteoblast tissue engineering applications... - Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope proteinKuen-Phon Wu
Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan 300, Republic of China
J Biol Chem 278:46007-13. 2003..Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses...