Research Topics
| Didier TronoSummaryAffiliation: University of Geneva Country: Switzerland Publications
| Collaborators
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Detail Information
Publications
Virology. Picking the right spotDidier Trono
Department of Genetics and Microbiology, University of Geneva, Switzerland
Science 300:1670-1. 2003- Retroviruses under editing crossfire: a second member of the human APOBEC3 family is a Vif-blockable innate antiretroviral factorDidier Trono
Department of Microbiology and Molecular Medicine, University of Geneva, CMU, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland
EMBO Rep 5:679-80. 2004 - Therapeutic lentivirus-mediated neonatal in vivo gene therapy in hyperbilirubinemic Gunn ratsTuan Huy Nguyen
Department of Microbiology and Molecular Medicine, CMU, University of Geneva, CH-1211 Geneva, Switzerland
Mol Ther 12:852-9. 2005..This work represents the first demonstration of a complete and permanent correction of hyperbilirubinemia in Gunn rats using lentiviral vectors... - Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal miceMike Themis
Gene Therapy Research Group, Section of Cell and Molecular Biology, Imperial College London, UK
Mol Ther 12:763-71. 2005..This system may provide a highly sensitive model to investigate integrating vector safety prior to clinical application... - Novel lentiviral vectors displaying "early-acting cytokines" selectively promote survival and transduction of NOD/SCID repopulating human hematopoietic stem cellsEls Verhoeyen
INSERM, U412, Lyon, France
Blood 106:3386-95. 2005..This was demonstrated by their capacity to promote selective transduction of CD34(+) cell in in vitro derived long-term culture-initiating cell (LTC-IC) colonies and of long-term NOD/SCID repopulating cells (SRCs) in vivo... - Lentiviral vectors and antiretroviral intrinsic immunityBastien Mangeat
School of Life Sciences and Frontiers in Genetics National Center for Competence in Research, Swiss Federal Institute of Technology, Lausanne, Switzerland
Hum Gene Ther 16:913-20. 2005..We present here the current state of knowledge on this rapidly evolving field and discuss implications for gene therapy... - Transduction of CpG DNA-stimulated primary human B cells with bicistronic lentivectorsKrisztian Kvell
Division of Hematology, Department of Internal Medicine, University Hospitals, 1211 Geneva 14, Switzerland
Mol Ther 12:892-9. 2005..This system should be useful for gene function research in primary B cells and development of gene therapies... - Development of cellular models for ribosomal protein S19 (RPS19)-deficient diamond-blackfan anemia using inducible expression of siRNA against RPS19Koichi Miyake
Molecular Medicine and Gene Therapy, Lund University, BMC A12, 221 84 Lund, Sweden
Mol Ther 11:627-37. 2005..These novel cell lines represent models for RPS19-deficient DBA and can be used to identify the molecular mechanisms in RPS19-deficient DBA... - Editing at the crossroad of innate and adaptive immunityPriscilla Turelli
School of Life Sciences, , CH-1015 Lausanne, Switzerland
Science 307:1061-5. 2005..Exciting questions are raised, which take us to the depth of the intimate relations between vertebrates and the microbial underworld... - DNA damage sensors ATM, ATR, DNA-PKcs, and PARP-1 are dispensable for human immunodeficiency virus type 1 integrationYasuo Ariumi
Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, 1211, Geneva 4, Switzerland
J Virol 79:2973-8. 2005..We conclude that the DNA repair enzymes ATM, ATR, DNA-PKcs, and PARP-1 are not essential for HIV-1 integration... - Harnessing HIV for therapy, basic research and biotechnologyMaciej Wiznerowicz
Frontiers in Genetics, National Center for Competence in Research, School of Life Sciences, Swiss Federal Institute of Technology, Laussane, Switzerland
Trends Biotechnol 23:42-7. 2005.... - Genotypic features of lentivirus transgenic miceMarc Olivier Sauvain
Ecole Polytechnique Federale de Lausanne, School of Life Sciences, Lausanne CH 1015, Switzerland
J Virol 82:7111-9. 2008.... - Treatment of acetaminophen-induced acute liver failure in the mouse with conditionally immortalized human hepatocytesTuan Huy Nguyen
Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland
J Hepatol 43:1031-7. 2005..CONCLUSIONS: Lentiviral vectors represent tools of choice for immortalization of non-dividing primary cells, and lentivirally immortalized human hepatocytes are promising reagents for cell-based therapy of acute liver failure... - A simple and highly effective method for the stable transduction of uncultured porcine hepatocytes using lentiviral vectorTuan Huy Nguyen
Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Switzerland
Cell Transplant 14:489-96. 2005..We demonstrated that porcine hepatocytes are highly susceptible to lentiviral vector and describe an easy methodology to efficiently, rapidly, and stably introduce transgenes into uncultured porcine hepatocytes... - Production and titration of lentiviral vectorsPatrick Salmon
Department of Neuroscience, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Curr Protoc Hum Genet . 2007..This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories... - Interfering residues narrow the spectrum of MLV restriction by human TRIM5alphaPierre V Maillard
Global Health Institute, School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, Lausanne, Switzerland
PLoS Pathog 3:e200. 2007.... - The Kruppel-associated box repressor domain can trigger de novo promoter methylation during mouse early embryogenesisMaciej Wiznerowicz
School of Life Sciences, Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland
J Biol Chem 282:34535-41. 2007..Here we reveal that, in sharp contrast, KRAB-mediated repression results in irreversible gene silencing through promoter DNA methylation if it acts during the first few days of mouse development... - Expression of FGF-2 in neural progenitor cells enhances their potential for cellular brain repair in the rodent cortexAlexandre G Dayer
Department of Neurosciences, University Medical Center, University of Geneva Medical School, Geneva, Switzerland
Brain 130:2962-76. 2007.... - Induction of antiviral cytidine deaminases does not explain the inhibition of hepatitis B virus replication by interferonsStephanie Jost
School of Life Sciences and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015 Lausanne, Switzerland
J Virol 81:10588-96. 2007..We conclude that these cytidine deaminases are not essential effectors of IFN in its action against this pathogen... - Inhibition of HIV-1 multiplication by a modified U7 snRNA inducing Tat and Rev exon skippingMaria B Asparuhova
Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland
J Gene Med 9:323-34. 2007..Therefore, this new approach targeting HIV-1 regulatory genes at the level of pre-mRNA splicing, in combination with other antiviral strategies, may be a useful new tool in the fight against HIV/AIDS... - Ex vivo lentivirus transduction and immediate transplantation of uncultured hepatocytes for treating hyperbilirubinemic Gunn ratTuan Huy Nguyen
Department of Microbiology and Molecular Medicine, University of Geneva Medical Center, Geneva, Switzerland, and Service de biochimie, Hopital Saint Joseph, Paris, France
Transplantation 82:794-803. 2006..Here, we evaluated the SLIT approach in Gunn rats, the animal model for Crigler-Najjar syndrome type 1, a defect in bilirubin UDP-glucuronosyltransferase (BUGT)... - KRAB can repress lentivirus proviral transcription independently of integration siteYannick Bulliard
School of Life Sciences, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
J Biol Chem 281:35742-6. 2006..These results indicate that KRAB-induced transcriptional repression is robust and active over a variety of genomic contexts that include at least the wide range of sites targeted by lentiviral integration... - The integrase interactor 1 (INI1) proteins facilitate Tat-mediated human immunodeficiency virus type 1 transcriptionYasuo Ariumi
Department of Microbiology and Molecular Medicine, University of Geneva and Frontiers in Genetics National Center for Competence in Research, Switzerland
Retrovirology 3:47. 2006..These results suggest that the incoming PICs might recruit INI1 to facilitate proviral transcription... - A versatile tool for conditional gene expression and knockdownJolanta Szulc
School of Life Sciences, , CH-1015 Lausanne, Switzerland
Nat Methods 3:109-16. 2006..These results open up promising perspectives for basic or translational research and for the development of gene-based therapeutics... - Deficiency of ribosomal protein S19 in CD34+ cells generated by siRNA blocks erythroid development and mimics defects seen in Diamond-Blackfan anemiaJohan Flygare
Molecular Medicine and Gene Therapy, Institute of Laboratory Medicine and The Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden
Blood 105:4627-34. 2005..This study shows for the first time that RPS19 silencing decreases the proliferative capacity of hematopoietic progenitors and leads to a defect in erythroid development... - APOBEC3G genetic variants and their influence on the progression to AIDSPing An
Basic Research Program, SAIC Frederick, National Cancer Institute Frederick Cancer Research and Development Center, Frederick, MD 21702, USA
J Virol 78:11070-6. 2004..These studies suggest that there may be a modifying role of variants of APOBEC3G on HIV-1 disease progression that warrants further investigation... - Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcriptsBastien Mangeat
Department of Genetics and Microbiology, University of Geneva, 1211 Geneva 4, Switzerland
Nature 424:99-103. 2003..We also find that APOBEC3G can act on a broad range of retroviruses in addition to HIV, suggesting that hypermutation by editing is a general innate defence mechanism against this important group of pathogens... - Highly efficient lentiviral vector-mediated transduction of nondividing, fully reimplantable primary hepatocytesTuan Huy Nguyen
Departments of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Switzerland
Mol Ther 6:199-209. 2002..These results have immediate implications for the treatment of liver diseases by the transplantation of genetically modified hepatocytes, an approach that could be applied to a number of hereditary and acquired hepatic disorders... - IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytesEls Verhoeyen
, , Lyon, France
Blood 101:2167-74. 2003..With adult naive T cells, preactivation with recombinant IL-7 was necessary for efficient gene transfer. Altogether, these results suggest that IL-7-displaying vectors could constitute interesting tools for T-cell-targeted gene therapy... - Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primatesVirginie Sandrin
, U412 INSERM, IFR 74, , Lyon, France
Blood 100:823-32. 2002..Furthermore, as compared to vectors pseudotyped with other retroviral GPs or with VSV-G, RD114/TR-pseudotyped vectors showed augmented transduction of human and macaque primary blood lymphocytes and CD34+ cells... - Transepithelial transport of HIV-1 by M cells is receptor-mediatedGrigorios Fotopoulos
Swiss Institute for Experimental Cancer Research and Institute of Biochemistry, University of Lausanne, CH 1066 Epalinges, Switzerland
Proc Natl Acad Sci U S A 99:9410-4. 2002..The follicle-associated epithelium covering human gut lymphoid follicles expresses CCR5, but not CXCR4, and lactosyl cerebroside, suggesting that HIV-1 infection may occur through M cells and enterocytes at these sites... - Lentivirus-mediated transduction of connexin cDNAs shows level- and isoform-specific alterations in insulin secretion of primary pancreatic beta-cellsDavid Caton
Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland
J Cell Sci 116:2285-94. 2003.... - Differentiation of the RN33B cell line into forebrain projection neurons after transplantation into the neonatal rat brainCecilia Lundberg
Wallenberg Neuroscience Center, Lund University, S-221 84, Sweden
Exp Neurol 175:370-87. 2002.... - A single amino acid determinant governs the species-specific sensitivity of APOBEC3G to Vif actionBastien Mangeat
Department of Microbiology and Molecular Medicine and Frontiers in Genetics Research Program, CMU, 1 rue Michel Servet, 1211 Geneva 4, Switzerland
J Biol Chem 279:14481-3. 2004..Furthermore, we show that this phenotype correlates with the ability of Vif to bind APOBEC3G and interfere with its incorporation into virions. These results shed light on an important determinant of the tropism of primate lentiviruses... - Inhibition of hepatitis B virus replication by APOBEC3GPriscilla Turelli
Department of Microbiology and Molecular Medicine and Frontiers in Genetics Research Program, University of Geneva, Switzerland
Science 303:1829. 2004 - Efficient transduction of primary human B lymphocytes and nondividing myeloma B cells with HIV-1-derived lentiviral vectorsFabrice Bovia
Division of Hematology, Department of Medicine, University Hospital, Geneva, Switzerland
Blood 101:1727-33. 2003..In conclusion, HIV vectors are promising tools for studies of gene functions in primary human B cells and myeloma cells for the purposes of research and the development of gene therapies... - Efficient gene transfer into human primary blood lymphocytes by surface-engineered lentiviral vectors that display a T cell-activating polypeptideMarielle Maurice
, , U412 INSERM, IFR 74 and ENS de Lyon, , 69364 Lyon Cedex 07, France
Blood 99:2342-50. 2002..Moreover these results provide a proof of concept for an approach that may have utility in various gene transfer applications, including in vivo gene delivery... - Lentiviral vector transduction of NOD/SCID repopulating cells results in multiple vector integrations per transduced cell: risk of insertional mutagenesisNiels-Bjarne Woods
Department for Molecular Medicine and Gene Therapy, Institute of Laboratory Medicine, Lund University, Sweden
Blood 101:1284-9. 2003..While this is efficient in terms of transduction and transgene expression, it may increase the risk of insertional mutagenesis... - Entry and transcription as key determinants of differences in CD4 T-cell permissiveness to human immunodeficiency virus type 1 infectionAngela Ciuffi
Institute of Microbiology, University of Lausanne, Switzerland
J Virol 78:10747-54. 2004..The phenotype of provirus transcription was stable over time, indicating that it represents a genetic trait... - Lentivirus-mediated RNA interference of DC-SIGN expression inhibits human immunodeficiency virus transmission from dendritic cells to T cellsJean Francois Arrighi
Department of Dermatology and Venereology, University Hospital of Geneva, 4 752, 24 Rue Micheli du Crest, 1211 Geneva, Switzerland
J Virol 78:10848-55. 2004..The present system should have broad applications for studying the function of DC-SIGN in the pathogenesis of HIV as well as other pathogens also recognized by this receptor... - Modalities of interleukin-7-induced human immunodeficiency virus permissiveness in quiescent T lymphocytesOdile Ducrey-Rundquist
Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Geneva, Switzerland
J Virol 76:9103-11. 2002.... - Lentivirus as a tool for lineage-specific gene manipulationsAnna Malashicheva
Department of Developmental Biology, Max Planck Institute for Immunobiology, Freiburg, Germany
Genesis 45:456-9. 2007..This LV feature will allow modulations of gene expression in a lineage-specific manner, thus having significant applications in mouse functional genetics... - The HIV-1 Nef protein and phagocyte NADPH oxidase activationFrederik Vilhardt
Biology of Ageing Laboratory, Department of Geriatrics, Geneva University Hospitals, Switzerland
J Biol Chem 277:42136-43. 2002..Rac activation is not sufficient to stimulate the phagocyte NADPH oxidase; however, it markedly enhances the NADPH oxidase response to other stimuli... - Antiprion prophylaxis by gene transfer of a soluble prion antagonistNicolas Genoud
Institute of Neuropathology, University Hospital Zurich, Zurich, Switzerland
Am J Pathol 172:1287-96. 2008..These results suggest that somatic gene transfer of prion antagonists may be effective for postexposure prophylaxis of prion diseases... - Production and titration of lentiviral vectorsPatrick Salmon
Department of Neuroscience, Faculty of Medicine, University of Geneva, Geneva, Switzerland
Curr Protoc Neurosci . 2006..This unit also describes protocols for production and titration of LVs that can be implemented in a research laboratory setting, with an emphasis on standardization to improve transposability of results between laboratories... - APOBEC3-independent interferon-induced viral clearance in hepatitis B virus transgenic micePriscilla Turelli
Global Health Institute, School of Life Sciences and Frontiers in Genetics National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne, CH 1015 Lausanne, Switzerland
J Virol 82:6585-90. 2008..We conclude that APOBEC3 is not an essential mediator of the IFN-mediated inhibition of HBV in vivo... - A human TRIM5alpha B30.2/SPRY domain mutant gains the ability to restrict and prematurely uncoat B-tropic murine leukemia virusFelipe Diaz-Griffero
Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Department of Pathology, Division of AIDS, Harvard Medical School, Boston, MA 02115, USA
Virology 378:233-42. 2008..Thus, the range of retroviruses restricted by human TRIM5alpha can be increased by changes in the B30.2/SPRY domain, which also result in the ability to cause premature uncoating of the restricted retroviral capsid... - Lentiviral vectors interfering with virus-induced CD4 down-modulation potently block human immunodeficiency virus type 1 replication in primary lymphocytesHang M Pham
La Jolla Institute for Molecular Medicine, 4570 Executive Dr, Suite 100, San Diego, CA 92121, USA
J Virol 78:13072-81. 2004..The findings presented here provide proof-of-principle that approaches targeting the virus-induced CD4 down-modulation may constitute the basis for novel anti-HIV therapies... - Tuning silence: conditional systems for RNA interferenceMaciej Wiznerowicz
School of Life Sciences, National Center for Competence in Research, , CH-1015 Lausanne, Switzerland
Nat Methods 3:682-8. 2006..Here we summarize the state of the art of systems allowing for drug-controllable knockdown, and provide a description of their current and future applications... - The innate antiretroviral factor APOBEC3G does not affect human LINE-1 retrotransposition in a cell culture assayPriscilla Turelli
Department of Microbiology and Molecular Medicine, University of Geneva, 1211 4, Switzerland
J Biol Chem 279:43371-3. 2004..We used such an assay to demonstrate that APOBEC3G, which impairs the replication of exogenous retroelements, does not affect the replication of endogenous L1 retrotransposons... - ARF1 regulates Nef-induced CD4 degradationJulien Faure
Department of Biochemistry, University of Geneva, 1211 Geneva, Switzerland
Curr Biol 14:1056-64. 2004..The HIV Nef protein downregulates CD4 through sequential connection with clathrin-coated pits and the COP1 coatomer, resulting in accelerated endocytosis and lysosomal targeting... - Contribution of proteoglycans to human immunodeficiency virus type 1 brain invasionMichael D Bobardt
Department of Immunology, IMM-9, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA
J Virol 78:6567-84. 2004.... - Statins reduce interleukin-6-induced C-reactive protein in human hepatocytes: new evidence for direct antiinflammatory effects of statinsClaire Arnaud
Division of Cardiology, Foundation for Medical Research, Faculty of Medicine, Geneva University Hospital, Switzerland
Arterioscler Thromb Vasc Biol 25:1231-6. 2005..We further show that statins act via inhibition of STAT3 phosphorylation. These findings furnish new evidence for direct antiinflammatory properties of statins and provide new mechanistic insight into their clinical benefits... - Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interferenceMaciej Wiznerowicz
Department of Genetics and Microbiology, CMU, Faculty of Medicine, University of Geneva, 1211 Geneva 4, Switzerland
J Virol 77:8957-61. 2003..As lentivirus vectors can stably transduce a wide variety of targets both in vitro and in vivo and can be used to generate transgenic animals, the present system should have broad applications... - Role for human immunodeficiency virus type 1 membrane cholesterol in viral internalizationMireille Guyader
Department of Genetics and Microbiology, University of Geneva, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland
J Virol 76:10356-64. 2002.... - Treatment of fulminant liver failure by transplantation of microencapsulated primary or immortalized xenogeneic hepatocytesGang Mai
Surgical Research Unit, Department of Surgery, University Hospital Geneva, Switzerland
Xenotransplantation 12:457-64. 2005.... - Hide, shield and strike back: how HIV-infected cells avoid immune eradicationB Matija Peterlin
Department of Medicine, Rosalind Russell Medical Research Center, University of California, San Francisco, California 94143 0703, USA
Nat Rev Immunol 3:97-107. 2003..A better understanding of these strategies might lead to new approaches in the fight against AIDS... - Ataxia-telangiectasia-mutated (ATM) protein can enhance human immunodeficiency virus type 1 replication by stimulating Rev functionYasuo Ariumi
Department of Microbiology and Molecular Medicine, University of Geneva, Switzerland
J Virol 80:2445-52. 2006..Altogether, these results indicate that ATM can positively influence HIV-1 Rev function... - Differentiation of trophoblast giant cells and their metabolic functions are dependent on peroxisome proliferator-activated receptor beta/deltaKarim Nadra
Center for Integrative Genomics, University of Lausanne, CH 1015 Lausanne, Switzerland
Mol Cell Biol 26:3266-81. 2006..Altogether, this important role of PPARbeta/delta in placenta development and giant cell differentiation should be considered when contemplating the potency of PPARbeta/delta agonist as therapeutic agents of broad application... - Multipotential nestin and Isl-1 positive mesenchymal stem cells isolated from human pancreatic isletsMichael Eberhardt
Division for Endocrinology, Diabetes and Clinical Nutrition, Department of Research, University Hospital Basel, Switzerland
Biochem Biophys Res Commun 345:1167-76. 2006..In conclusion, cultured pancreatic islets contain nestin and Isl-1 positive mesenchymal stem cells with multipotential developmental capacity... - Characterization of APOBEC3G binding to 7SL RNADaniel Bach
Global Health Institute, School of Life Sciences and Frontiers in Genetics, National Center for Competence in Research, Ecole Polytechnique Federale de Lausanne EPFL, CH 1015, Lausanne, Switzerland
Retrovirology 5:54. 2008..We thus conclude that 7SL RNA is not an essential mediator of the virion packaging of these antiviral cytidine deaminases...