Research Topics
| Alexandra TrkolaSummaryAffiliation: University Hospital Country: Switzerland Publications
| Collaborators
|
Detail Information
Publications
HIV-1 escape from a small molecule, CCR5-specific entry inhibitor does not involve CXCR4 useAlexandra Trkola
Division of Infectious Diseases, Department of Medicine, University Hospital Zurich, 8091 Zurich, Switzerland
Proc Natl Acad Sci U S A 99:395-400. 2002..Instead, HIV-1 acquires the ability to use CCR5 despite the inhibitor, first by requiring lower levels of CCR5 for entry and then probably by using the drug-bound form of the receptor...
Delay of HIV-1 rebound after cessation of antiretroviral therapy through passive transfer of human neutralizing antibodiesAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
Nat Med 11:615-22. 2005....
In vivo efficacy of human immunodeficiency virus neutralizing antibodies: estimates for protective titersAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
J Virol 82:1591-9. 2008..Equally, this raises hopes that a preventive vaccine could become effective at comparatively lower neutralizing antibody titers...
HIV-host interactions: vital to the virus and key to its inhibitionAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, 8091 Zurich, Switzerland
Curr Opin Microbiol 7:555-9. 2004..Nevertheless, current knowledge on the molecular interactions between HIV and host-cell factors has substantially broadened our understanding of the viral life cycle and opened new investigative areas for drug intervention...
HIV-host interactions: vital to the virus and key to its inhibitionAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, 8091 Zurich, Switzerland
Curr Opin Microbiol 7:407-11. 2004..Nevertheless, current knowledge on the molecular interactions between HIV and host-cell factors has substantially broadened our understanding of the viral life cycle and opened new investigative areas for drug intervention...
Potent human immunodeficiency virus-neutralizing and complement lysis activities of antibodies are not obligatorily linkedMichael Huber
Division of Infectious Diseases, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
J Virol 82:3834-42. 2008..In summary, our data support the notion that the in vivo activities of 2G12, 2F5, and 4E10 are likely due to direct neutralization or Fc receptor-mediated mechanisms such as phagocytosis and antibody-dependent cellular cytotoxicity...
Humoral immunity to HIV-1: kinetics of antibody responses in chronic infection reflects capacity of immune system to improve viral set pointAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
Blood 104:1784-92. 2004..In summary, measuring the kinetics of antibody responses provided a marker to validate the responsiveness and capacities of the immune system of HIV-1-infected individuals and reflected the patients' ability to decrease viral set points...
Human immunodeficiency virus type 1 fitness is a determining factor in viral rebound and set point in chronic infectionAlexandra Trkola
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, 8091 Zurich, Switzerland
J Virol 77:13146-55. 2003....
Complement lysis activity in autologous plasma is associated with lower viral loads during the acute phase of HIV-1 infectionMichael Huber
Division of Infectious Diseases, , , Switzerland
PLoS Med 3:e441. 2006....
Association between specific HIV-1 Env traits and virologic control in vivoBeda Joos
University Hospital Zurich, Department of Medicine, Switzerland
Infect Genet Evol 10:365-72. 2010..These residues in HIV gp120 might affect in vivo HIV-1 fitness either at the level of Env function or influence susceptibility to adaptive or innate immune response...
HIV rebounds from latently infected cells, rather than from continuing low-level replicationBeda Joos
Department of Medicine, Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, CH 8091 Zurich, Switzerland
Proc Natl Acad Sci U S A 105:16725-30. 2008..Furthermore, a prolonged delay in restoration of pretreatment viral diversity after treatment interruption demonstrates a surprisingly sustained evolutionary bottleneck induced by punctuated antiretroviral therapy...
Shifts in cell-associated HIV-1 RNA but not in episomal HIV-1 DNA correlate with new cycles of HIV-1 infection in vivoMarek Fischer
Division of Infectious Diseases and Hospital Epidemiology, Department of Medicine, University Hospital Zurich, 8091 Zurich, Switzerland
Antivir Ther 8:97-104. 2003..In contrast, expression of HIV-UsRNAPBMC increased during STI and consequently provides a more sensitive, albeit not absolute cellular marker of ongoing HIV-1 replication...
Low human immunodeficiency virus envelope diversity correlates with low in vitro replication capacity and predicts spontaneous control of plasma viremia after treatment interruptionsBeda Joos
University Hospital Zurich, Department of Medicine, Division of Infectious Diseases and Hospital Epidemiology, , , Switzerland
J Virol 79:9026-37. 2005....
Virus isolates during acute and chronic human immunodeficiency virus type 1 infection show distinct patterns of sensitivity to entry inhibitorsPeter Rusert
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
J Virol 79:8454-69. 2005..Activities of these MAbs correlated significantly with each other, suggesting that common features of the viral envelope modulate their potencies...
Positive in vivo selection of the HIV-1 envelope protein gp120 occurs at surface-exposed regionsBeda Joos
Department of Medicine, Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Zurich, CH 8091, Switzerland
J Infect Dis 196:313-20. 2007....
Long-term multiple-dose pharmacokinetics of human monoclonal antibodies (MAbs) against human immunodeficiency virus type 1 envelope gp120 (MAb 2G12) and gp41 (MAbs 4E10 and 2F5)Beda Joos
University Hospital Zurich, Infectious Diseases and Hospital Epidemiology, Ramistrasse 100, CH 8091 Zurich, Switzerland
Antimicrob Agents Chemother 50:1773-9. 2006..Further studies examining tissue concentrations to explain the differential in vivo activity of the anti-gp120 antibody compared with those of the two anti-gp41 antibodies are warranted...
In vivo and in vitro escape from neutralizing antibodies 2G12, 2F5, and 4E10Amapola Manrique
Division of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, Ramistrasse 100, 8091 Zurich, Switzerland
J Virol 81:8793-808. 2007..This remarkable vulnerability of the virus to interference within the MPER calls for a further evaluation of the safety and efficacy of MPER-targeting therapeutic and vaccination strategies...
Quantification of infectious HIV-1 plasma viral load using a boosted in vitro infection protocolPeter Rusert
Division of Infectious Diseases, University Hospital Zurich, Switzerland
Virology 326:113-29. 2004..Measuring viral infectivity may thereby function as an additional, useful marker in monitoring disease progression and evaluating efficacy of antivirals in vivo...
Residual cell-associated unspliced HIV-1 RNA in peripheral blood of patients on potent antiretroviral therapy represents intracellular transcriptsMarek Fischer
Department of Medicine, University Hospital Zurich, Switzerland
Antivir Ther 7:91-103. 2002..As shown by limiting dilution analysis, HIV-1 infected cells with such a repressed viral transcription pattern were observed at high frequencies in PBMC from untreated patients...
HIV RNA in plasma rebounds within days during structured treatment interruptionsMarek Fischer
Division of Infectious Diseases, , Switzerland
AIDS 17:195-9. 2003....
HIV-1 superinfection in an HIV-2-infected woman with subsequent control of HIV-1 plasma viremiaHuldrych F Gunthard
Division of Infectious Diseases and Hospital Epidemiology, University of Zurich, Zurich, Switzerland
Clin Infect Dis 48:e117-20. 2009..This evidence supports a mechanism other than cross-neutralizing antibodies for the mild course of HIV-1 infection in this woman...
CD4-specific designed ankyrin repeat proteins are novel potent HIV entry inhibitors with unique characteristicsAndreas Schweizer
Division of Infectious Diseases, University Hospital Zurich, Zurich, Switzerland
PLoS Pathog 4:e1000109. 2008....
Divergent effects of cell environment on HIV entry inhibitor activityPeter Rusert
Institute of Medical Virology, University of Zurich, University Hospital Zurich, Switzerland
AIDS 23:1319-27. 2009..In the present study, we investigate the influence the target cell context bears on HIV entry inhibition...
MPER-specific antibodies induce gp120 shedding and irreversibly neutralize HIV-1Claudia R Ruprecht
Institute of Medical Virology, University Hospital Zurich, Switzerland
J Exp Med 208:439-54. 2011....
RANTES (CCL5) uses the proteoglycan CD44 as an auxiliary receptor to mediate cellular activation signals and HIV-1 enhancementBranka Roscic-Mrkic
Division of Infectious Diseases, Department of Medicine, University Hospital, Zurich, Switzerland
Blood 102:1169-77. 2003..Here we show that silencing of CD44 in HeLa-CD4 cells prevents the activation of p44/p42 MAPK and leads to a substantial reduction in HIV-1 infectivity enhancement by RANTES...
The CCR5 and CXCR4 coreceptors are both used by human immunodeficiency virus type 1 primary isolates from subtype CTonie Cilliers
AIDS Virus Research Unit, National Institute for Communicable Diseases, Johannesburg, South Africa
J Virol 77:4449-56. 2003..These observations are relevant to understanding the rapid spread of HIV-1 subtype C in the developing world and to the design of intervention and treatment strategies...
Analysis of the mechanism by which the small-molecule CCR5 antagonists SCH-351125 and SCH-350581 inhibit human immunodeficiency virus type 1 entryFotini Tsamis
Microbiology and Immunology Department, Albert Einstein College of Medicine, Bronx, New York 10461, USA
J Virol 77:5201-8. 2003..We propose that the binding of small molecules to the transmembrane domain of CCR5 may disrupt the conformation of its extracellular domain, thereby inhibiting ligand binding to CCR5...
The differential sensitivity of human and rhesus macaque CCR5 to small-molecule inhibitors of human immunodeficiency virus type 1 entry is explained by a single amino acid difference and suggests a mechanism of action for these inhibitorsErika Billick
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, New York 10021, USA
J Virol 78:4134-44. 2004..However, SCH-C is unable to exert this effect on CCR5 conformation when residue 198 is methionine. The region of CCR5 near residue 198 has, therefore, an important influence on the conformational state of this receptor...
Loss of viral control in early HIV-1 infection is temporally associated with sequential escape from CD8+ T cell responses and decrease in HIV-1-specific CD4+ and CD8+ T cell frequenciesAnnette Oxenius
Institute for Microbiology, Eidgenossische Technische Hochschule, Zurich, Switzerland
J Infect Dis 190:713-21. 2004..These findings demonstrate the temporal relationship between viral escape from CD8+ T cell activity, decrease in HIV-1-specific T cell frequencies, and loss of control of viral replication...
Use of alternate coreceptors on primary cells by two HIV-1 isolatesTonie Cilliers
AIDS Virus Research Unit, National Institute for Communicable Diseases, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
Virology 339:136-44. 2005..Collectively, these data suggest that some HIV-1 isolates can use alternate coreceptors on primary cells, which may have implications for strategies that aim to block viral entry...
Interaction of the CC-chemokine RANTES with glycosaminoglycans activates a p44/p42 mitogen-activated protein kinase-dependent signaling pathway and enhances human immunodeficiency virus type 1 infectivityTheresa Li-Yun Chang
Department of Microbiology and Immunology, Weill Medical College of Cornell University, New York, USA
J Virol 76:2245-54. 2002..Here we show that activation of both PTK and MAPK is involved in the enhancement of HIV-1 infectivity caused by RANTES in cells that lack GPCRs for RANTES but which express GAGs...
A plea for justice for jailed medical workersSunil K Ahuja
Science 314:924-5. 2006
