P GONCZY

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..Our findings demonstrate that ZYG-8 is a MAP crucial for proper spindle positioning in C. elegans, and indicate that the function of the Doublecortin domain in modulating microtubule dynamics is conserved across metazoan evolution...

..The findings of two recent studies suggest a novel mechanism for nuclear envelope breakdown in which cytoplasmic dynein anchored on the outside of the nucleus generates tension, thus triggering tearing of the nuclear envelope...

..This article discusses recent findings that indicate how this mechanism might be used for spindle positioning during Drosophila and Caenorhabditis elegans development...

..The results suggest a novel mechanism for the tight coupling between these two organelles, and shed new light on its biological significance...

..Together, these experimental approaches contribute to a better understanding of the mechanisms orchestrating spatial cell division control in metazoan organisms...

..Together, our findings support a model in which centrosomes serve as integrative centers for mitotic regulators and thus trigger mitotic entry in a timely fashion...

..Our findings suggest a mechanism in which RIC-8 favors generation of Galpha free from Gbetagamma and enables GPR-1/2 to mediate asymmetric cell division...

..Together with microtubule dynamics, this allows pulling forces to be exerted and proper cell division to be achieved...

..How anterior-posterior (A-P) polarity cues control this asynchrony remains to be elucidated...

..Lessons learned from these two model organisms also apply to cells that divide asymmetrically in other metazoans, such as self-renewing stem cells in mammals...

..Our results show that ooc-3 is required to form the cortical domains essential for polarity after cell division...

..These results suggest that dynein and Lis1, albeit functioning in identical processes, are targeted partially independently of one another...

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..Taken together, these findings indicate that ZYG-9-TAC-1 and ZYG-8 act in a partially redundant manner to ensure correct microtubule assembly throughout the cell cycle of early C. elegans embryos...

..Thus, the extent of net pulling forces may depend on cortical Galpha activity, which is regulated by anterior-posterior polarity cues through GPR-1/2...

..Several of these proteins have homologs in other metazoans, and we expect the assembly pathway that stems from our work to be conserved...

..While a large body of work has made important contributions to our understanding of the mechanisms governing microtubule dynamics in vitro, much remains to be learned about how these mechanisms operate in vivo...

..Our findings provide the first insights into the role of a member of the PP6 family of phosphatases in metazoan development...

..Therefore, HsSAS-6 is also required for centrosome duplication, indicating that the function of SAS-6-related proteins has been widely conserved during evolution...

..Additional evidence indicates that SAS-4 is recruited to the daughter centriole or a closely associated structure. These findings identify SAS-4 recruitment as a key step in the centrosome duplication cycle...

..This in turn leads to multipolar spindle assembly and cytokinesis defects. Overall, our findings suggest that centriole length must be carefully regulated to restrict procentriole number and thus ensure accurate cell division...

..Overall, our findings establish that phosphorylation of the evolutionarily conserved protein SAS-6 is critical for centriole formation and thus for faithful cell division...

..Here, we review recent findings that provide important mechanistic insights into how a single procentriole forms next to each centriole once per cell cycle, thus ensuring that one centrosome becomes two...

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