Research Topics
| Hans FredlundSummaryAffiliation: Orebro University Hospital Country: Sweden Publications
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Detail Information
Publications
Molecular genetic methods for diagnosis and characterisation of Chlamydia trachomatis and Neisseria gonorrhoeae: impact on epidemiological surveillance and interventionsHans Fredlund
Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 112:771-84. 2004..trachomatis. This is due to the low genetic variability and hence the limited benefits for partner notification. However, genotyping of C. trachomatis may play an important role under special circumstances...- A case of diphtheria in Sweden, October 2011H Fredlund
Orebro University Hospital, Department of Laboratory Medicine, Clinical Microbiology, Orebro, Sweden
Euro Surveill 16:20038. 2011..diphtheriae. No secondary cases were found. A high level of adherence to childhood vaccination programmes is an effective way to protect populations against diphtheria... - Characteristics of Neisseria meningitidis isolates causing fatal diseaseSusanne Jacobsson
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Scand J Infect Dis 40:734-44. 2008.... - A comparative study of three different PCR assays for detection of Mycoplasma genitalium in urogenital specimens from men and womenAndreas Edberg
Department of Clinical Microbiology, Central Hospital, Karlstad, Sweden
J Med Microbiol 57:304-9. 2008..genitalium DNA. Real-time MgPa gene PCR is well suited for the clinical diagnosis of M. genitalium... - Combined real-time PCR and pyrosequencing strategy for objective, sensitive, specific, and high-throughput identification of reduced susceptibility to penicillins in Neisseria meningitidisSara Thulin
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Antimicrob Agents Chemother 52:753-6. 2008..All results were in concordance with Sanger sequencing, and a high correlation between alterations in the two Pen(i)-specific sites and reduced susceptibility to penicillins was identified... - Endocervical swabs transported in first void urine as combined specimens in the detection of Mycoplasma genitalium by real-time PCRAndreas Edberg
Department of Clinical Microbiology, Central Hospital, SE 651 85 Karlstad, Sweden
J Med Microbiol 58:117-20. 2009..genitalium DNA. Moreover, automated BioRobot M48 extraction was shown to be superior to a crude manual Chelex extraction, leaving no PCR inhibition and giving a slightly higher DNA yield and/or better sensitivity... - Emergence, spread and characteristics of Neisseria gonorrhoeae isolates with in vitro decreased susceptibility and resistance to extended-spectrum cephalosporins in SwedenDaniel Golparian
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Sex Transm Infect 86:454-60. 2010.... - Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variantMargaretha Jurstrand
Clinical Research Centre, Orebro University Hospital, Orebro SE 70185, Sweden
Sex Transm Infect 86:56-60. 2010..The study also aimed to evaluate the discriminatory capacity of multilocus sequence typing (MLST) compared with ompA sequencing... - Antibiotic susceptibility and characteristics of Neisseria meningitidis isolates from the African meningitis belt, 2000 to 2006: phenotypic and genotypic perspectivesSara Thulin Hedberg
Department of Laboratory Medicine, National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 53:1561-6. 2009..Regarding beta-lactam antibiotics, rare isolates showed a reduced susceptibility to penicillins, but the expanded-spectrum cephalosporins are not affected at present... - Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime and ceftriaxone: association with genetic polymorphisms in penA, mtrR, porB1b, and ponARobert Lindberg
Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
Antimicrob Agents Chemother 51:2117-22. 2007..In conclusion, an unambiguous association between penA mosaic alleles, in conjunction with genetic polymorphisms in mtrR, porB1b, and ponA, and greater reduced susceptibility to cefixime and ceftriaxone was identified... - Molecular characterization of Neisseria gonorrhoeae identifies transmission and resistance of one ciprofloxacin-resistant strainMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 115:231-41. 2007..gonorrhoeae in the effective treatment of gonorrhoea... - Antibiotic susceptibility of invasive Neisseria meningitidis isolates from 1995 to 2008 in Sweden--the meningococcal population remains susceptibleSara Thulin Hedberg
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Scand J Infect Dis 42:61-4. 2010..In general, these remain highly susceptible to the antibiotics recommended for use. Accordingly, penicillin G remains effective for the treatment of invasive meningococcal disease and ciprofloxacin appropriate for prophylaxis... - Genotyping of Chlamydia trachomatis would improve contact tracingLars Falk
Department of Dermatology and Venerology, Orebro University Hospital, Orebro, Sweden
Sex Transm Dis 30:205-10. 2003..The reported number of Chlamydia trachomatis genital infections has increased 15% annually since 1997 in Sweden. Inaccurate partner notification might be one reason... - Comparison of serologic and genetic porB-based typing of Neisseria gonorrhoeae: consequences for future characterizationMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
J Clin Microbiol 41:4141-7. 2003..In conclusion, analysis of short highly variable regions of the porB gene could form the basis for a fast molecular epidemiological tool for the examination of emergence and transmission of N. gonorrhoeae strains within the community... - The Neisseria gonorrhoeae population in Sweden during 2005-phenotypes, genotypes and antibiotic resistanceBirgitta Olsen
Department of Clinical Medicine, Orebro University, Orebro, Sweden
APMIS 116:181-9. 2008..gonorrhoeae strains circulating in our societies, is crucial... - Emergence of the new variant of Chlamydia trachomatis in a defined area of Sweden before 2002?Margaretha Jurstrand
Clinical Research Centre, Orebro University Hospital, Orebro, Sweden
Sex Transm Infect 86:337-41. 2010.... - Prevalence, phenotypic and genetic characteristics of prolyliminopeptidase-negative Neisseria gonorrhoeae isolates in Sweden during 2000-2007Emma Johansson
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
APMIS 117:900-4. 2009..Wherever high reliance is placed on PIP activity for N. gonorrhoeae species confirmation, changes in the diagnostic strategies may need to be considered and/or monitoring of the PIP activity is crucial... - Phenotypic and genetic characterization of the 2008 WHO Neisseria gonorrhoeae reference strain panel intended for global quality assurance and quality control of gonococcal antimicrobial resistance surveillance for public health purposesMagnus Unemo
Department of Clinical Microbiology, Orebro University Hospital, Sweden
J Antimicrob Chemother 63:1142-51. 2009..Here we phenotypically and genetically characterized the 2008 WHO N. gonorrhoeae reference panel, widely used under existing WHO AMR surveillance protocols... - Molecular epidemiology of Neisseria gonorrhoeae: sequence analysis of the porB gene confirms presence of two circulating strainsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
J Clin Microbiol 40:3741-9. 2002..gonorrhoeae strains, as well as for confirmation or discrimination of clusters of gonorrhea cases... - Molecular typing of Neisseria gonorrhoeae isolates by pyrosequencing of highly polymorphic segments of the porB geneMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, SE 701 85 Orebro, Sweden
J Clin Microbiol 42:2926-34. 2004..In conclusion, the fast and high-throughput pyrosequencing technology can be used for molecular epidemiological characterization of N. gonorrhoeae... - Detection of Mycoplasma genitalium in urogenital specimens by real-time PCR and by conventional PCR assayMargaretha Jurstrand
Department of Clinical Microbiology, Outpatient Sexually Transmitted Disease Clinic, Orebro University Hospital, SE 70185 Orebro, Sweden
J Med Microbiol 54:23-9. 2005..With improvement in sample preparation methods and increased volumes of the template DNA, the LC-PCR assay could be a useful routine diagnostic method... - Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samplesKristoffer Stralin
Department of Infectious Diseases, Orebro University Hospital, Orebro, Sweden
APMIS 113:99-111. 2005..In conclusion, the mPCR appears to be a promising tool in the aetiological diagnostics of LRTI... - Total variation in the penA gene of Neisseria meningitidis: correlation between susceptibility to beta-lactam antibiotics and penA gene heterogeneitySara Thulin
National Reference Laboratory for Pathogenic Neisseria, Department of Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Antimicrob Agents Chemother 50:3317-24. 2006..By studying the penA gene, genotypical determination of susceptibility in culture-negative cases can also be accomplished... - The porA pseudogene of Neisseria gonorrhoeae--low level of genetic polymorphism and a few, mainly identical, inactivating mutationsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Orebro University Hospital, Orebro, Sweden
APMIS 113:410-9. 2005..The highly conserved N. gonorrhoeae porA pseudogene may reflect an evolutionary neutral molecular clock and may be a suitable genetic target for diagnosis of N. gonorrhoeae... - The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterizationMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine, Orebro University Hospital, Orebro, Sweden
Microbiology 156:1394-404. 2010..Therefore, the rapid transmission of nvCT in Sweden was due to the strong diagnostic selective advantage and its introduction into a high-frequency transmitting population... - Defective neutrophil oxidative burst in preterm newborns on exposure to coagulase-negative staphylococciMaria Björkqvist
Department of Pediatrics, Orebro University Hospital, S 701 85 Orebro, Sweden
Pediatr Res 55:966-71. 2004..It is concluded that the baseline oxidative activity is similar in term and preterm PMNLs but that preterm PMNLs have a decreased capacity to increase the oxidative burst in response to bacterial stimulation... - A serological study of the role of Mycoplasma genitalium in pelvic inflammatory disease and ectopic pregnancyMargaretha Jurstrand
Clinical Research Centre, University Hospital, SE 70185 Orebro, Sweden
Sex Transm Infect 83:319-23. 2007..The aim of this study was to evaluate the association between M genitalium antibodies and pelvic inflammatory disease (PID) and ectopic pregnancy (EP) using a lipid-associated membrane protein-enzyme immunoassay (LAMP-EIA) method... - Isolation and characterization of Neisseria meningitidis in the vaccine era. Who needs what and when?Per Olcen
National Reference Laboratory for Pathogenic Neisseria, Department of Laboratory Medicine Clinical Microbiology, Orebro University Hospital, Orebro, Sweden
Scand J Infect Dis 42:4-11. 2010..Such an approach will make use of the best qualities of both systems. Who needs what and when concerning the characterization is outlined, including future situations when new meningococcal vaccines are available... - Pulsed-field gel electrophoresis as an epidemiologic tool for Neisseria gonorrhoeae: identification of clusters within serovarsMagnus Unemo
National Reference Laboratory for Pathogenic Neisseria and Unit for Infectious Disease Control, Department of Clinical Microbiology and Immunology, Orebro Medical Centre Hospital, Orebro, Sweden
Sex Transm Dis 29:25-31. 2002..The increasing incidence of gonorrhea in Sweden in 1998 was due to mostly domestic cases. Among these, two core groups were identified: homosexual men with serovar IB-2 and young heterosexuals with serovar IB-3... - Sputum specimens can be obtained from patients with community-acquired pneumonia in primary careFolke Lagerström
Varberga Health Centre, Orebro, Sweden
Scand J Prim Health Care 22:83-6. 2004..To improve the aetiological diagnosis in community-acquired pneumonia (CAP) increased efforts were made to obtain expectorated sputum specimens from patients with CAP... - Alcohol-based hand-disinfection reduced children's absence from Swedish day care centersAnne Lennell
Department of Clinical Microbiology, Unit for Infectious Disease Control, Orebro University Hospital, Orebro University, Orebro, Sweden
Acta Paediatr 97:1672-80. 2008..To determine if the use of alcohol-based hand-disinfection as a complement to regular hand washing at daycare centers (DCCs) can reduce the childhood rate of absenteeism... - Atypical clinical presentation of laboratory-acquired meningococcal diseaseSimon Athlin
Department of Infectious Diseases, University Hospital, Orebro, Sweden
Scand J Infect Dis 39:911-3. 2007..This case had an atypical clinical picture, exemplifies the use of non-culture methods for diagnosis and characterization, and reminds us about the importance of safe routines for the laboratory work... - One year of Neisseria gonorrhoeae isolates in Sweden: the prevalence study of antibiotic susceptibility shows relation to the geographic area of exposureTorsten Berglund
Unit for Infectious Disease Epidemiology, Microbiology and Tumour Biology Centre, Karolinska Institute, Stockholm, Sweden
Int J STD AIDS 13:109-14. 2002..5% of the isolates from patients exposed in other places (RR=8.5, P<0.001). Ciprofloxacin cannot be recommended as the first choice of treatment if the place of exposure was in Asia... - [Increasing incidence of Chlamydia infections in Sweden. Shortcomings in primary health care management]Fredrik Landström
Lakartidningen 99:2950-2. 2002..This must improve. Generous testing of asymptomatic women is supported by this study. Chlamydia trachomatis infection should be considered in patients with cystitis. Voluntary testing in men should be encouraged... - Pyrosequencing of the DNA gyrase gene in Neisseria species: effective indicator of ciprofloxacin resistance in Neisseria gonorrhoeaeEmma Lindback
Department of Clinical Bacteriology, Division of Laboratory Medicine, Karolinska University Hospital Huddinge, Stockholm, Sweden
APMIS 114:837-41. 2006..gonorrhoeae and seems unsuitable for species verification of N. gonorrhoeae. However, whether it is also possible to use this region for verification depends on the specificity of the primary screening method used... - A premature infant with fulminant meningococcal septicaemiaBo Selander
Department of Paediatrics, Lund University Hospital, Lund, Sweden
Scand J Infect Dis 35:345-8. 2003..2%) were asymptomatic carriers of N. meningitidis. Serotyping and pulsed-field gel electrophoresis of the genomic DNA of the N. meningitidis isolates revealed that the infant and his father had closely related strains...