Research Topics
Genomes and GenesSpecies | Anders HelanderSummaryAffiliation: Karolinska Institutet Country: Sweden Publications
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Detail Information
Publications
Testing for congenital disorders of glycosylation by HPLC measurement of serum transferrin glycoformsAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet and University Hospital, Stockholm, Sweden
Clin Chem 50:954-8. 2004
Mass spectrometric identification of ethyl sulfate as an ethanol metabolite in humansAnders Helander
Karolinska Institutet and University Hospital, Department of Clinical Neuroscience, Stockholm, Sweden
Clin Chem 50:936-7. 2004
Comparison of HPLC and capillary electrophoresis for confirmatory testing of the alcohol misuse marker carbohydrate-deficient transferrinAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet and University Hospital, Stockholm, Sweden
Clin Chem 51:1528-31. 2005
Laboratory tests for acute alcohol consumption: results of the WHO/ISBRA Study on State and Trait Markers of Alcohol Use and DependenceAnders Helander
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden
Alcohol Clin Exp Res 26:1070-7. 2002..Comparison was made with self-reported drinking levels...
Ethyl sulfate: a metabolite of ethanol in humans and a potential biomarker of acute alcohol intakeAnders Helander
Department of Clinical Neuroscience and University Hospital, Stockholm, Sweden
J Anal Toxicol 29:270-4. 2005..It is also indicated that the concurrent determination of EtS and EtG will improve sensitivity, when being used as biomarkers of recent drinking...
Urinary tract infection: a risk factor for false-negative urinary ethyl glucuronide but not ethyl sulfate in the detection of recent alcohol consumptionAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet and University Hospital, Stockholm, Sweden
Clin Chem 51:1728-30. 2005
Improved HPLC method for carbohydrate-deficient transferrin in serumAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet and Hospital, SE 171 76 Stockholm, Sweden
Clin Chem 49:1881-90. 2003..We describe an improved HPLC method for transferrin glycoforms...
Detection times for urinary ethyl glucuronide and ethyl sulfate in heavy drinkers during alcohol detoxificationAnders Helander
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden
Alcohol Alcohol 44:55-61. 2009..This study determined the detection times for EtG and EtS in alcoholic patients undergoing alcohol detoxification...
Urinary ethyl glucuronide and ethyl sulfate testing for recent drinking in alcohol-dependent outpatients treated with acamprosate or placeboHelen Dahl
Alcohol Laboratory, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Alcohol Alcohol 46:553-7. 2011..This study compared urinary EtG and EtS measurement with self-reports for detection of prior drinking in alcohol-dependent outpatients treated with the anti-craving medication acamprosate or placebo...
Alcohol biomarker analysis: simultaneous determination of 5-hydroxytryptophol glucuronide and 5-hydroxyindoleacetic acid by direct injection of urine using ultra-performance liquid chromatography-tandem mass spectrometryNikolai Stephanson
Department of Medicine, Division of Clinical Pharmacology, Karolinska Institute and University Hospital, SE 171 76 Stockholm, Sweden
J Mass Spectrom 42:940-9. 2007..In conclusion, a fully validated and robust direct method for the routine measurement of urinary GTOL and 5-HIAA was developed...
Biomarkers to disclose recent intake of alcohol: potential of 5-hydroxytryptophol glucuronide testing using new direct UPLC-tandem MS and ELISA methodsOlof Beck
Department of Medicine, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden
Alcohol Alcohol 42:321-5. 2007..This study compared two new methods for direct determination of 5-hydroxytryptophol glucuronide (GTOL) in urine, a biomarker for detection of recent alcohol consumption...
Unreliable alcohol testing in a shipping safety programmeAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
Forensic Sci Int 189:e45-7. 2009..This and other questionable cases prompted investigation of the suitability of urine alcohol testing for the intended application...
Screening and brief intervention for risky alcohol consumption in the workplace: results of a 1-year randomized controlled studyUlric Hermansson
Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
Alcohol Alcohol 45:252-7. 2010..To assess the effectiveness of brief alcohol intervention on hazardous and harmful drinking in the 12-month period after a voluntary alcohol screening...
Comparison of urinary excretion characteristics of ethanol and ethyl glucuronideHelen Dahl
Department of Clinical Neuroscience, Karolinska Institute and Hospital, Stockholm, Sweden
J Anal Toxicol 26:201-4. 2002..Moreover, it was possible to lower the concentration of EtG by drinking large amounts of water prior to voiding, whereas this strategy did not influence the EtG/creatinine ratio or the concentration of ethanol...
Screening for high-risk and elevated alcohol consumption in day and shift workers by use of the AUDIT and CDTUlric Hermansson
Karolinska Institutet, Department of Clinical Neuroscience, Center for Dependency Disorders, Karolinska Hospital, Stockholm, Sweden
Occup Med (Lond) 53:518-26. 2003..However, no studies have evaluated the Alcohol Use Disorders Identification Test (AUDIT) or the alcohol biomarker carbohydrate-deficient transferrin (CDT) in serum for this purpose...
Bioanalytical investigation of asarone in connection with Acorus calamus oil intoxicationsKristian Björnstad
Alcohol Laboratory, Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
J Anal Toxicol 33:604-9. 2009..calamus. The main clinical symptom reported by the patients was prolonged vomiting that sometimes lasted more than 15 h...
Postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumptionAnders Helander
Department of Clinical Neuroscience, Karolinska Institute, Karolinska University Hospital, Stockholm, Sweden
Clin Chem 53:1855-7. 2007..This study examined if bacterial pathogens in urine may enable postsampling synthesis of EtG and ethyl sulfate (EtS) from ethanol, leading to clinical false-positive results...
Influence of alcohol use, ethnicity, age, gender, BMI and smoking on the serum transferrin glycoform pattern: implications for use of carbohydrate-deficient transferrin (CDT) as alcohol biomarkerJonas P Bergström
Department of Clinical Neuroscience, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden
Clin Chim Acta 388:59-67. 2008....
HPLC evaluation of clinical and pharmacological factors reported to cause false-positive carbohydrate-deficient transferrin (CDT) levelsJonas P Bergström
Department of Clinical Neuroscience, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden
Clin Chim Acta 389:164-6. 2008..This HPLC study evaluated some clinical conditions and medications previously suggested to interfere with and cause non-alcohol related elevations of CDT...
Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: II. Performance of a laboratory network running the HPLC candidate reference measurement procedure and evaluation of a candidate reference materialAnders Helander
Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Clin Chem Lab Med 48:1585-92. 2010..9% to 5.5%. The logistics for distribution of reference materials and review of results were found to be functional, indicating that a full reference system for CDT may soon be available...
Changes in transferrin glycosylation during pregnancy may lead to false-positive carbohydrate-deficient transferrin (CDT) results in testing for riskful alcohol consumptionNaama Kenan
Alcohol Laboratory, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Clin Chim Acta 412:129-33. 2011..This study examined the transferrin glycosylation profile and the risk for false-positive CDT results during pregnancy...
Determination of carbohydrate-deficient transferrin in human serum using the Bio-Rad %CDT by HPLC testAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden
Clin Chim Acta 371:187-90. 2006..This study evaluated the performance of a new commercial method for CDT, the Bio-Rad %CDT by HPLC test, that measures relative amounts of separate transferrin glycoforms in proportion to total transferrin...
Clinical characteristics of carbohydrate-deficient transferrin (%disialotransferrin) measured by HPLC: sensitivity, specificity, gender effects, and relationship with other alcohol biomarkersJonas P Bergström
Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
Alcohol Alcohol 43:436-41. 2008....
Comparison of analytical approaches for liquid chromatography/mass spectrometry determination of the alcohol biomarker ethyl glucuronide in urineAnders Helander
Karolinska Institutet, Department of Laboratory Medicine, Stockholm, Sweden
Rapid Commun Mass Spectrom 24:1737-43. 2010..These results indicate that the likelihood of different analytical methods to provide reliable analytical results depends on the reporting limit applied...
A multi-component LC-MS/MS method for detection of ten plant-derived psychoactive substances in urineKristian Björnstad
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden
J Chromatogr B Analyt Technol Biomed Life Sci 877:1162-8. 2009..The multi-component method proved to be useful for investigation of authentic cases of intoxication with plant-derived psychoactive drugs and was indicated to cover the clinically relevant concentration ranges...
5-hydroxytryptophol as a marker for recent alcohol intakeOlof Beck
Department of Medicine, Division of Clinical Pharmacology, Karolinska Hospital, Stockholm, Sweden
Addiction 98:63-72. 2003..To review the mechanism behind the alcohol-induced shift in serotonin metabolism, and the use of urinary 5-hydroxytryptophol (5-HTOL) as a biochemical marker of acute alcohol consumption...
Direct quantification of ethyl glucuronide in clinical urine samples by liquid chromatography-mass spectrometryNikolai Stephanson
Department of Medicine, Division of Clinical Pharmacology, Alcohol Laboratory, Karolinska Institute and Hospital, Stockholm, Sweden
Ther Drug Monit 24:645-51. 2002....
Bioanalytical and clinical evaluation of 103 suspected cases of intoxications with psychoactive plant materialsKristian Björnstad
Department of Clinical Neuroscience, Alcohol Laboratory, Karolinska Institutet, Stockholm, Sweden
Clin Toxicol (Phila) 47:566-72. 2009..This study involved bioanalytical and clinical examinations of intoxication cases suspected to be linked to such plant materials...
Method development for routine liquid chromatography-mass spectrometry measurement of the alcohol biomarker phosphatidylethanol (PEth) in bloodYufang Zheng
Alcohol Laboratory, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Clin Chim Acta 412:1428-35. 2011..Measurement of PEth in whole blood samples is employed as an alcohol biomarker. This work aimed to further develop an LC-MS method for PEth to make it practical for routine laboratory use...
Solid-phase extraction procedure for ethyl glucuronide in urineYufang Zheng
Department of Clinical Neuroscience, Karolinska Institutet and Alcohol Laboratory, Karolinska University Hospital, Stockholm, Sweden
J Anal Toxicol 32:778-81. 2008..The urinary EtG results with SPE followed by LC-MS were highly correlated (r(2) = 0.959) with those obtained using a sensitive and selective ultra-performance LC-tandem MS method...
Molecular species of the alcohol biomarker phosphatidylethanol in human blood measured by LC-MSAnders Helander
Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden
Clin Chem 55:1395-405. 2009..We developed an electrospray ionization (ESI) LC-MS method for qualitative and quantitative measurement of different PEth species in human blood...
Development and clinical application of an LC-MS-MS method for mescaline in urineKristian Björnstad
Department of Clinical Neuroscience, Karolinska Institute and Karolinska University Hospital, Stockholm, Sweden
J Anal Toxicol 32:227-31. 2008..Among 462 urine samples collected from young people with alcohol or drug problems, 32% were positive for illicit drugs, but none for mescaline...
A comprehensive workplace intervention and its outcome with regard to lifestyle, health and sick leave: the AHA studyGunnar Bergström
Section for Personal Injury Prevention, Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden
Work 31:167-80. 2008..This comprehensive workplace intervention programme appears to have had positive effects on smoking habits, HRQoL and sick leave...
Importance of HPLC confirmation of problematic carbohydrate-deficient transferrin (CDT) results from a multicapillary electrophoresis routine methodNaama Kenan
Alcohol Laboratory, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Clin Chim Acta 411:1945-50. 2010....
The Alcohol Use Disorders Identification Test and carbohydrate-deficient transferrin in alcohol-related sickness absenceUlric Hermansson
Karolinska Institute, Department of Clinical Neuroscience, Center for Dependency Disorders, Karolinska Hospital, Stockholm, Sweden
Alcohol Clin Exp Res 26:28-35. 2002..The purpose of this study was to examine the relationship between sick-days, 12 months before screening, and the AUDIT and CDT (CDTect kit). Serum gamma-glutamyltransferase also was used for comparison...
Urinary excretion patterns of 5-hydroxyindole-3-acetic acid and 5-hydroxytryptophol in various animal species: implications for studies on serotonin metabolism and turnover rateMargareta Some
Department of Clinical Neuroscience, Alcohol Laboratory L7 03, Karolinska Institutet and Hospital, SE 171 76, Stockholm, Sweden
Life Sci 71:2341-9. 2002....
Multicentre validation study of instrument applications for %CDT, an immunoassay for quantification of carbohydrate-deficient transferrin in serumAnders Helander
Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden
Alcohol Alcohol 37:209-12. 2002..The data indicate that transferability of the %CDT assay is high, and that the instrument applications may be used interchangeably in routine quantification of %CDT...
Urinary ethyl glucuronide and ethyl sulfate testing for detection of recent drinking in an outpatient treatment program for alcohol and drug dependenceHelen Dahl
Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden
Alcohol Alcohol 46:278-82. 2011..This study determined the information about recent alcohol consumption obtained when urinary ethyl glucuronide (EtG) and ethyl sulfate (EtS) were introduced as a routine test in outpatient treatment programs for alcohol and drug dependence...
Urinary ethyl glucuronide and 5-hydroxytryptophol levels during repeated ethanol ingestion in healthy human subjectsTaisto Sarkola
Department of Mental Health and Alcohol Research, National Public Health Institute, POB 33, Fin 00251 Helsinki, Finland
Alcohol Alcohol 38:347-51. 2003....
[5-HTOL--a new biochemical alcohol marker with forensic applications]Anders Helander
Beroendecentrum, Karolinska sjukhuset och Karolinska institutet, Stockholm
Lakartidningen 99:3950-4. 2002..Examples include suspected drunk drivers, rape victims, and medico-legal autopsies where forensic ethanol analysis is requested...
CDT, GGT, and AST as markers of alcohol use: the WHO/ISBRA collaborative projectKatherine M Conigrave
Drug and Alcohol Department, Royal Prince Alfred Hospital, Missenden Road, Camperdown, NSW 2050, Sydney, Australia
Alcohol Clin Exp Res 26:332-9. 2002..The WHO/ISBRA Collaborative Study allows assessment and comparison of CDT, GGT, and aspartate aminotransferase (AST) as markers of drinking in a large, well-characterized, multicenter sample...
HPLC and mass spectrometric characterization of a candidate reference material for the alcohol biomarker carbohydrate-deficient transferrin (CDT)Walter Oberrauch
Bio Rad Laboratories GmbH, Munich, Germany
Clin Chim Acta 395:142-5. 2008..This study characterized a candidate reference material for CDT measurement derived from isolated human transferrin glycoforms...
Insufficient standardization of a direct carbohydrate-deficient transferrin immunoassayAnders Helander
Clin Chem 54:1090-2. 2008
Urinary ethyl glucuronide testing detects alcohol consumption in alcoholic liver disease patients awaiting liver transplantationYesim Erim
Department of Psychosomatic Medicine and Psychotherapy, University Hospital of Essen, Germany
Liver Transpl 13:757-61. 2007....
Development and multicenter evaluation of the N latex CDT direct immunonephelometric assay for serum carbohydrate-deficient transferrinJoris R Delanghe
Department of Clinical Chemistry, Ghent University Hospital, Ghent, Belgium
Clin Chem 53:1115-21. 2007..We describe the development and multicenter evaluation of N Latex CDT (Dade Behring), an automated, particle-enhanced, homogeneous immunonephelometric assay for directly determining CDT...
A pharmacokinetic study of ethyl glucuronide in blood and urine: applications to forensic toxicologyGudrun Høiseth
Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug Abuse, Nydalen, Oslo, Norway
Forensic Sci Int 172:119-24. 2007..For this purpose, two subsequent increasing EtG values and a high ethanol-to-EtG ratio in blood would support information of recent drinking...
Evaluation of a new immunoassay for urinary ethyl glucuronide testingMichael Böttcher
Arztpraxis für Medizinische Mikrobiologie und Labordiagnostik, Dessau, Germany
Alcohol Alcohol 43:46-8. 2008..This study evaluated a new enzyme immunoassay (DRI-EtG EIA, Microgenics Corp.) for determination of the EtG concentration in urine samples...
Comparison of ethyl glucuronide in hair with phosphatidylethanol in whole blood as post-mortem markers of alcohol abusePeter Bendroth
Department of Forensic Medicine, SE 223 62 Lund, Sweden
Forensic Sci Int 176:76-81. 2008..However, an EtG level below the cutoff does not completely exclude previous alcohol abuse...
Value of ethyl glucuronide in plasma as a biomarker for recent alcohol consumption in the emergency roomTim Neumann
Department of Anesthesiology and Intensive Care Medicine, Charite Universitatsmedizin Berlin, Germany
Alcohol Alcohol 43:431-5. 2008....
Determination of urinary 5-hydroxytryptophol glucuronide by liquid chromatography-mass spectrometryNikolai Stephanson
Department of Medicine, Division of Clinical Pharmacology, Karolinska Institute and University Hospital, SE 17176 Stockholm, Sweden
J Chromatogr B Analyt Technol Biomed Life Sci 816:107-12. 2005..99, n=70; mean 5-HTOL/GTOL ratio=1.10). This is the first direct assay for quantification of GTOL in urine. The method is suitable for routine application...
Comparison between the urinary alcohol markers EtG, EtS, and GTOL/5-HIAA in a controlled drinking experimentGudrun Høiseth
Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug Abuse, Pb 4404 Nydalen, N 0403 Oslo, Norway
Alcohol Alcohol 43:187-91. 2008..The aim of this controlled study was to compare the sensitivities and detection times of EtG, EtS, and GTOL/5-HIAA, after a single ingestion of ethanol...
The effects of self-administered alcohol-induced 'hangover' in a naturalistic setting on psychomotor and cognitive performance and subjective stateFrances Finnigan
Department of Psychology, Glasgow Caledonian University, Glasgow, UK
Addiction 100:1680-9. 2005..To examine in as naturalistic a setting as possible whether having an alcohol-induced 'hangover' impairs psychomotor and cognitive performance...
The relationship between whole-blood serotonin and subjective mood in apparently healthy postmenopausal womenMaresa E Duffy
The Northern Ireland Centre for Food and Health (NICHE, University of Ulster, Coleraine, Northern Ireland, UK
Biol Psychol 73:165-8. 2006..03). No association was apparent for negative affect. These findings indicate that WB 5-HT and positive affect are related in postmenopausal women...
[Positive alcohol breath test following methadone solution]Anders Helander
Beroendecentrum,
Lakartidningen 104:2217-8; discussion 2218. 2007
[Final reply: the level of ethanol in Metadon DnE can surely be a problem]Anders Helander
Lakartidningen 104:2450. 2007
Effect of folic acid supplementation on mood and serotonin response in healthy malesEmma Williams
Northern Ireland Centre for Food and Health (NICHE, University of Ulster, Coleraine BT52 1SA, Northern Ireland
Br J Nutr 94:602-8. 2005..Further research is needed to address the effect of folic acid supplementation or of longer duration or increased dose, particularly in the face of sub-optimal folate status...
Multicentre evaluation of a new assay for determination of carbohydrate-deficient transferrinMarkus J Schwarz
, , Germany
Alcohol Alcohol 38:270-5. 2003..09% CDT, correlation 0.984). Good agreement was also found with the Axis-Shield %CDT microtitre test. CONCLUSIONS: Data from this study indicate that reliable, well standardized %CDT results are obtained using the new assay...
Toward standardization of carbohydrate-deficient transferrin (CDT) measurements: I. Analyte definition and proposal of a candidate reference methodJan Olof Jeppsson
Malmo University Hospital, Malmo, Sweden
Clin Chem Lab Med 45:558-62. 2007..In clinical use, CDT should be expressed in a relative amount (% CDT), to compensate for variations in the total transferrin concentration...
