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Genomes and GenesSpecies | A KarlssonSummaryCountry: Sweden Publications
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Publications
The two neutrophil members of the formylpeptide receptor family activate the NADPH-oxidase through signals that differ in sensitivity to a gelsolin derived phosphoinositide-binding peptideHuamei Fu
Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
BMC Cell Biol 5:50. 2004....- Cytochalasin B triggers a novel pertussis toxin sensitive pathway in TNF-alpha primed neutrophilsJohan Bylund
Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
BMC Cell Biol 5:21. 2004..The receptor uncoupling results in respiratory burst activity when signals generated by reactivated formyl peptide receptors trigger the NADPH-oxidase to produce superoxide anions... - Phorbol myristate acetate induces neutrophil NADPH-oxidase activity by two separate signal transduction pathways: dependent or independent of phosphatidylinositol 3-kinaseA Karlsson
Department of Medical Microbiology and Immunology, University of Goteborg, Sweden
J Leukoc Biol 67:396-404. 2000..As the two oxidase responses were differently affected by the inhibitor Gö-6850, different PKC isozymes are suggested to take part in the two signal transduction pathways... - Wheat germ agglutinin induces NADPH-oxidase activity in human neutrophils by interaction with mobilizable receptorsA Karlsson
The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Goteborg, Sweden
Infect Immun 67:3461-8. 1999..The extra- and intracellular NADPH-oxidase responses showed differences in sialic acid dependency, indicating that these two responses are mediated by different receptor structures... - Assembly and activation of the neutrophil NADPH oxidase in granule membranesAnna Karlsson
The Phagocyte Research Laboratory, Department of Rheumatology, University of Goteborg, S 413 46 Goteborg, Sweden
Antioxid Redox Signal 4:49-60. 2002..Signaling capabilities of the oxidants, affecting for example, the state of protein phosphorylation, regulation of transcription factors, and induction of apoptosis, are discussed... - The synthetic chemoattractant Trp-Lys-Tyr-Met-Val-DMet activates neutrophils preferentially through the lipoxin A(4) receptorC Dahlgren
The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Goteborg, Sweden
Blood 95:1810-8. 2000..The observation that exudated cells are primed in their response to WKYMVm suggests that FPRL1/LXA(4)R like FPR is stored in mobilizable organelles. (Blood. 2000;95:1810-1818).. - Proinflammatory activity of a cecropin-like antibacterial peptide from Helicobacter pyloriJ Bylund
The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Goteborg, Sweden
Antimicrob Agents Chemother 45:1700-4. 2001..Furthermore, we show that these effects are mediated through binding of Hp(2-20) to the promiscuous, G-protein-linked lipoxin A(4) receptor-formyl peptide-like receptor 1... - Activation of human neutrophils by mycobacterial phenolic glycolipidsJ Faldt
Department of Medical Microbiology and Immunology, Goteborg University, Goteborg, Sweden
Clin Exp Immunol 118:253-60. 1999..The subcellular localization of the reactive oxygen metabolites formed was related to the way in which the glycolipids were presented to the cells... - The phagocyte chemiluminescence paradox: luminol can act as an inhibitor of neutrophil NADPH-oxidase activityJ Faldt
Department of Medical Microbiology and Immunology, Goteborg University, Guldhedsgatan 10, S 413 46 Goteborg, Sweden
Luminescence 14:153-60. 1999..As a consequence of the present results, showing that luminol but not isoluminol can inhibit NADPH-oxidase activity, we suggest that isoluminol is used in future studies of superoxide anion release from phagocytes... - Identification of CD66a and CD66b as the major galectin-3 receptor candidates in human neutrophilsE Feuk-Lagerstedt
The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Sweden
J Immunol 163:5592-8. 1999..This conclusion was supported by the subcellular localization of the CD66 proteins to the gelatinase and specific granules in resting neutrophils... - Priming of human neutrophils by mycobacterial lipoarabinomannans: role of granule mobilisationJ Faldt
Department of Medical Microbiology and Immunology, Goteborg University, Box 435, 405 30, Goteborg, Sweden
Microbes Infect 3:1101-9. 2001.... - The synthetic peptide Trp-Lys-Tyr-Met-Val-Met-NH2 specifically activates neutrophils through FPRL1/lipoxin A4 receptors and is an agonist for the orphan monocyte-expressed chemoattractant receptor FPRL2T Christophe
Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, S 40530 Göteborg, Sweden
J Biol Chem 276:21585-93. 2001..Taken together, the results indicate that neutrophils are activated by WKYMVM through FPRL1 and that FPRL2 is a chemotactic receptor transducing signals in myeloid cells... - Different glycosphingolipid composition in human neutrophil subcellular compartmentsA Karlsson
Department of Medical Microbiology and Immunology, , P.O. Box 435, , Sweden
Glycoconj J 18:231-43. 2001..Accordingly, we show that neutrophil granules store specific carbohydrate epitopes that are upregulated to the plasma membrane upon cell activation... - Respiratory burst in human neutrophilsC Dahlgren
The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Guldhedsgatan 10, S 413 46, Goteborg, Sweden
J Immunol Methods 232:3-14. 1999..The basic principles for luminol/isoluminol-amplified chemiluminescence is used as the starting point for discussing methodological problems related to measurements of oxygen metabolites generated by professional phagocytes... - Neutrophil secretory vesicles are the intracellular reservoir for GPI-80, a protein with adhesion-regulating potentialC Dahlgren
Department of Medical Microbiology and Immunology, University of Goteborg, Sweden
J Leukoc Biol 69:57-62. 2001..Thus, we identify secretory vesicles as the reservoir of GPI-80 from which it may translocate to the plasma membrane after weak stimulation of the cells... - The lysosomal membrane glycoproteins Lamp-1 and Lamp-2 are present in mobilizable organelles, but are absent from the azurophil granules of human neutrophilsC Dahlgren
Department of Medical Microbiology and Immunology, University of Götenborg, Sweden
Biochem J 311:667-74. 1995..During phagocytosis both Lamp-1 and Lamp-2 became markedly concentrated around the ingested particle and they both appear on the cell surface when the secretory organelles are mobilized... - Interleukin-8-derived peptide has antibacterial activityAse Björstad
Department of Rheumatology and Inflammation Research, University of Goteborg, Guldhedsgatan 10, S 413 46 Goteborg, Sweden
Antimicrob Agents Chemother 49:3889-95. 2005..The results presented are of special interest when put in the context of the suggested importance of antimicrobial peptides for microbial colonization of the gastric mucosa... - Immunostimulatory oligodeoxynucleotides induce dolphin neutrophil NADPH-oxidase activation in a CpG-independent but phosphorothioate backbone-dependent mannerTakuya Itou
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
Dev Comp Immunol 29:583-8. 2005..These results reveal that PS-ODNs induce dolphin neutrophil NADPH-oxidase activation in a CpG motif-independent but phosphorothioate-dependent manner... - A proinflammatory peptide from herpes simplex virus type 2 glycoprotein G affects neutrophil, monocyte, and NK cell functionsLars Bellner
Department of Rheumatology and Inflammation Research, Goteborg University, Goteborg, Sweden
J Immunol 174:2235-41. 2005..We propose that the proinflammatory gG-2p20 peptide identified could contribute to a reduced function and viability of NK cells during HSV-2 infection due to its ability to recruit and activate phagocytic cells... - Newcastle disease virus neuraminidase primes neutrophils for stimulation by galectin-3 and formyl-Met-Leu-PheJenny Almkvist
The Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, , Sweden
Exp Cell Res 298:74-82. 2004..Since not only the galectin response but also the response to the chemoattractant fMLF was primed, NDV-NA appears to induce a general priming phenomenon, possibly due to receptor upregulation by degranulation... - Localization of human neutrophil interleukin-8 (CXCL-8) to organelle(s) distinct from the classical granules and secretory vesiclesSara Pellmé
University of Goteborg, Guldhedsgatan 10, S 413 46 Goteborg, Sweden
J Leukoc Biol 79:564-73. 2006..Upon fractionation of the cytoplasts, CXCL-8 was found to partly cofractionate with calnexin, a marker for endoplasmic reticulum (ER). Thus, part of CXCL-8 may be localized to the ER or ER-like structures in the neutrophil... - Changes in activation states of murine polymorphonuclear leukocytes (PMN) during inflammation: a comparison of bone marrow and peritoneal exudate PMNTakuya Itou
Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252 8510, Japan
Clin Vaccine Immunol 13:575-83. 2006..These results have major implications for murine neutrophil research and show the importance of defining which PMN subsets to use when investigating murine models... - The two neutrophil plasma membrane markers alkaline phosphatase and HLA class I antigen localize differently in granule-deficient cytoplasts. An ideal plasma membrane marker in human neutrophils is still lackingSara Pellmé
The Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
J Immunol Methods 325:88-95. 2007.... - Ability of monocyte-derived dendritic cells to secrete oxygen radicals in response to formyl peptide receptor family agonists compared to that of myeloid and plasmacytoid dendritic cellsAnna Karlsson
Department of Rheumatology and Inflammation Research, Guldhedsgatan 10A, 413 46 Goteborg, Sweden
Clin Vaccine Immunol 14:328-30. 2007.... - A monocyte-specific peptide from herpes simplex virus type 2 glycoprotein G activates the NADPH-oxidase but not chemotaxis through a G-protein-coupled receptor distinct from the members of the formyl peptide receptor familyLars Bellner
Department of Rheumatology and Inflammation Research, Goteborg University, Goteborg, Sweden
J Immunol 179:6080-7. 2007.... - Serum amyloid A mediates human neutrophil production of reactive oxygen species through a receptor independent of formyl peptide receptor like-1Lena Björkman
Department of Rheumatology and Inflammation Research, The Sahlgrenska Academy at Goteborg University, Goteborg, Sweden
J Leukoc Biol 83:245-53. 2008..Future identification and characterization of the SAA receptor could lead to development of novel, therapeutic targets for treatment of RA... - Changes in the ratio between FPR and FPRL1 triggered superoxide production in human neutrophils-a tool in analysing receptor specific eventsHuamei Fu
Department of Rheumatology and Inflammation Research, University of Goteborg, Guldhedsgatan 10, S 413 46 Goteborg, Sweden
J Immunol Methods 331:50-8. 2008.... - Measurement of respiratory burst products generated by professional phagocytesClaes Dahlgren
Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden
Methods Mol Biol 412:349-63. 2007..These techniques can be valuable tools in research spanning from basic phagocyte biology to more clinically-oriented research on innate immune mechanisms and inflammation... - The mechanism for activation of the neutrophil NADPH-oxidase by the peptides formyl-Met-Leu-Phe and Trp-Lys-Tyr-Met-Val-Met differs from that for interleukin-8Huamei Fu
Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden
Immunology 112:201-10. 2004..The data presented in this study provide support for two different signalling pathways to the neutrophil NADPH-oxidase, used by ligand binding to FPR/FPRL1 or CXCR, respectively... - Galectins as inflammatory mediatorsJenny Almkvist
The Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, , Sweden
Glycoconj J 19:575-81. 2004.... - Ionomycin-induced neutrophil NADPH oxidase activity is selectively inhibited by the serine protease inhibitor diisopropyl fluorophosphateClaes Dahlgren
The Phagocyte Research Laboratory, Department of Rheumatology, University of Goteborg, Goteborg, Sweden
Antioxid Redox Signal 4:17-25. 2002..We thus suggest that a serine protease is of importance for the calcium ionophore-induced signal(s) to reach and activate the dormant NADPH oxidase in the neutrophil granules... - Activation of the neutrophil nicotinamide adenine dinucleotide phosphate oxidase by galectin-1Jenny Almkvist
Department of Medical Microbiology and Immunology and Rheumatology, Phagocyte Research Laboratory, Goteborg University, Goteborg, Sweden
J Immunol 168:4034-41. 2002..In conclusion, galectin-1 appears to have proinflammatory functions, mediated through activation of the neutrophil respiratory burst... - Lipopolysaccharide-induced granule mobilization and priming of the neutrophil response to Helicobacter pylori peptide Hp(2-20), which activates formyl peptide receptor-like 1Johan Bylund
Phagocyte Research Laboratory, Department of Rheumatology, , , Sweden
Infect Immun 70:2908-14. 2002..These results suggest that the mechanism behind the LPS-induced priming of FPRL1-mediated responses lies at the level of granule (receptor) mobilization... - Helicobacter pylori induce neutrophil transendothelial migration: role of the bacterial HP-NAPMikael Brisslert
Department of Medical Microbiology and Immunology, Goteborg University, Sweden
FEMS Microbiol Lett 249:95-103. 2005..pylori induced neutrophil transendothelial migration. We propose that HP-NAP contributes to the continuous recruitment of neutrophils to the gastric mucosa of H. pylori infected individuals... - NADPH-oxidase activation in murine neutrophils via formyl peptide receptorsJohan Bylund
Department of Rheumatology and Inflammation Research, Goteborg University, Sweden
Exp Cell Res 282:70-7. 2003..Finally, we show that a synthetic hexapeptide, WKYMVM, is a more potent stimulus than fMLF for murine neutrophils and that these two agonists probably act via nonidentical high-affinity receptors... - Reactivation of formyl peptide receptors triggers the neutrophil NADPH-oxidase but not a transient rise in intracellular calciumJohan Bylund
Department of Rheumatology and Inflammation Research, , Guldhedsgatan 10, , Sweden
J Biol Chem 278:30578-86. 2003..Further, phosphatidylinositol 3-kinase, mitogen-activated protein kinase, and MEK all participated in the generation of second messengers from the reactivated receptors... - Capacitative Ca2+ influx and activation of the neutrophil respiratory burst. Different regulation of plasma membrane- and granule-localized NADPH-oxidaseDaniel Granfeldt
The Phagocyte Research Laboratory, Department of Rheumatology, , Sweden
J Leukoc Biol 71:611-7. 2002..Further, activation of the plasma membrane-localized NADPH-oxidase is regulated by a more complex Ca2+ signaling, involving capacitative Ca2+ influx and possibly the specific action of La3+-sensitive Ca2+ channels... - Secretion of heparin-binding protein from human neutrophils is determined by its localization in azurophilic granules and secretory vesiclesHans Tapper
Department of Cell and Molecular Biology, Lund University, Sweden
Blood 99:1785-93. 2002..The ability of neutrophils to secrete HBP from secretory vesicles may be important for proinflammatory functions of this protein, such as the alteration of vascular permeability... - Synaptotagmin II could confer Ca(2+) sensitivity to phagocytosis in human neutrophilsI Maria Lindmark
Division of Medical Microbiology IMK, Linkoping University, SE 581 85, Linkoping, Sweden
Biochim Biophys Acta 1590:159-66. 2002..Under calcium-reduced conditions, neither synaptotagmin II nor LAMP-1 translocated to the phagosome. We therefore suggest a role for synaptotagmin II as calcium-sensor during phagocytosis and secretion in neutrophils... - Immunostimulatory DNA induces degranulation and NADPH-oxidase activation in human neutrophils while concomitantly inhibiting chemotaxis and phagocytosisJohan Bylund
Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, , Guldhedsgatan 10, , Sweden
Eur J Immunol 32:2847-56. 2002..These findings may have implications for the development of DNA-based immunotherapy and vaccination... - Novel selective human mitochondrial kinase inhibitors: design, synthesis and enzymatic activityNunzia Ciliberti
Department of Pharmaceutical Sciences, University of Ferrara, Italy
Bioorg Med Chem 15:3065-81. 2007..The biological activity of a representative compound, 2'-O-decanoyl-BVaraU, was also investigated in normal human fibroblasts and was found to impair mitochondrial function due to TK2 inhibition... - Affinity of galectin-8 and its carbohydrate recognition domains for ligands in solution and at the cell surfaceSusanne Carlsson
Section of Microbiology Immunology and Glycobiology MIG, Solvegatan 23, 223 62, Sweden
Glycobiology 17:663-76. 2007..Also galectin-8 binding and signaling at cell surfaces can be explained by combined binding of the two CRDs to low or medium affinity ligands, and their highest affinity ligands, such as sialylated galactosides, are not required... - Depletion of mitochondrial DNA by down-regulation of deoxyguanosine kinase expression in non-proliferating HeLa cellsMaribel Franco
Karolinska Institute, Mitochondrial Medicine Center, Stockholm, Sweden
Exp Cell Res 313:2687-94. 2007..Our data also suggest that alterations in the expression level of DGUOK may induce compensatory changes in the expression of other nucleoside kinases... - The interaction between plasminogen and antiplasmin variants as studied by surface plasmon resonanceHaiyao Wang
Department of Clinical Chemistry and Blood Coagulation, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden
Biochim Biophys Acta 1764:1730-4. 2006..Thus, also the data obtained with surface plasmon resonance show that Lys436 indeed is very important in the lysine-binding site mediated interaction between plasminogen and antiplasmin... - Substrate specificity and phosphorylation of antiviral and anticancer nucleoside analogues by human deoxyribonucleoside kinases and ribonucleoside kinasesAn R Van Rompay
Department of Nephrology-Hypertension, University of Antwerp, 2610 Antwerp, Belgium
Pharmacol Ther 100:119-39. 2003..This overview is focused on the substrate specificity, tissue distribution, and subcellular location of the mammalian dNKs and rNKs and their role in the activation of NAs... - Human UMP-CMP kinase 2, a novel nucleoside monophosphate kinase localized in mitochondriaYunjian Xu
Department of Laboratory Medicine, Karolinska Institute, Stockholm 14186, Sweden
J Biol Chem 283:1563-71. 2008.... - Nelarabine: a new purine analog in the treatment of hematologic malignanciesSophie Curbo
Karolinska Institute, Department of Laboratory Medicine, Division of Metabolic Disorders, Novum, Huddinge, Sweden
Rev Recent Clin Trials 1:185-92. 2006..Here we present an overview of what is known about the mechanism of action of nelarabine and its status in clinical trials... - N1-substituted thymine derivatives as mitochondrial thymidine kinase (TK-2) inhibitorsAna-Isabel Hernandez
, Juan de la Cierva 3, E-28006 Madrid, Spain
J Med Chem 49:7766-73. 2006.... - Lipid raft proteome of the human neutrophil azurophil granuleElisabeth Feuk-Lagerstedt
School of Engineering, University College of Boras, Boras, Sweden
Proteomics 7:194-205. 2007.... - The Drosophila melanogaster UMP-CMP kinase cDNA encodes an N-terminal mitochondrial import signalSophie Curbo
Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, S 14186 Stockholm, Sweden
Biochem Biophys Res Commun 311:440-5. 2003..This is the first time a mitochondrial pyrimidine nucleoside monophosphate kinase has been cloned from any organism and we discuss the implication of this finding for deoxyribonucleoside salvage in both Drosophila and other organisms... - Active site mutants of Drosophila melanogaster multisubstrate deoxyribonucleoside kinaseNicola Solaroli
Division of Clinical Virology F68, Karolinska Institute, Huddinge University Hospital, Stockholm, Sweden and Dipartimento di Scienze Farmaceutiche, Universita di Ferrara, Italy
Eur J Biochem 270:2879-84. 2003.... - Non-nucleoside inhibitors of mitochondrial thymidine kinase (TK-2) differentially inhibit the closely related herpes simplex virus type 1 TK and Drosophila melanogaster multifunctional deoxynucleoside kinaseJan Balzarini
Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, Belgium
Mol Pharmacol 63:263-70. 2003..KIN-12, and particularly KIN-52, are the very first non-nucleoside specific inhibitors of TK-2 reported and may be useful for studying the physiological role of the mitochondrial TK-2 enzyme... - Phosphorylation of 9-beta-D-arabinofuranosylguanine monophosphate by Drosophila melanogaster guanylate kinaseMagnus Johansson
Karolinska Institute, Department of Laboratory Medicine, Novum, S 141 57 Stockholm, Sweden
Biochem Pharmacol 70:987-92. 2005..We used the enzyme to reconstitute the complete in vitro three-step phosphorylation pathway for the conversion of dGuo and araG to the corresponding triphosphates... - The presence of stomatin in detergent-insoluble domains of neutrophil granule membranesElisabeth Feuk-Lagerstedt
The Phagocyte Research Laboratory, Department of Rheumatology and Inflammation Research, Goteborg University, Sweden
J Leukoc Biol 72:970-7. 2002..We also show the presence of detergent-insoluble, low-density membrane domains in the plasma membrane and the granule membranes and found stomatin to be localized to these domains... - Ligand recognition and activation of formyl peptide receptors in neutrophilsHuamei Fu
Department of Rheumatology and Inflammation Research, , Sweden
J Leukoc Biol 79:247-56. 2006 - Uric acid, a nucleic acid degradation product, down-regulates dsRNA-triggered arthritisFariba Zare
Department of Rheumatology and Inflammation Research, University of Goteborg, Sweden
J Leukoc Biol 79:482-8. 2006..These findings demonstrate that uric acid displays a potent, distant anti-inflammatory effect in vivo. This property seems to be mediated by down-regulation of neutrophil influx to the site of inflammatory insult... - Engineering of a single conserved amino acid residue of herpes simplex virus type 1 thymidine kinase allows a predominant shift from pyrimidine to purine nucleoside phosphorylationJan Balzarini
Rega Institute for Medical Research, K U Leuven, B 3000 Leuven, Belgium
J Biol Chem 281:19273-9. 2006.... - Structure of vaccinia virus thymidine kinase in complex with dTTP: insights for drug designKamel El Omari
Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford OX3 7BN, UK
BMC Struct Biol 6:22. 2006..Vaccinia virus TK is a close orthologue of variola TK but also shares a relatively high sequence identity to human type 2 TK (hTK), thus achieving drug selectivity relative to the host enzyme is challenging... - Pharmacological and genetic inhibition of NADPH oxidase does not reduce brain damage in different models of perinatal brain injury in newborn miceChristina Doverhag
Department of Physiology and Neuroscience, Perinatal Center, Sahlgrenska Academy, Goteborg University, Sweden
Neurobiol Dis 31:133-44. 2008..The ROS-generating enzyme NADPH oxidase (Nox2) is present in inflammatory cells and contributes to brain injury in adult animal models...