Research Topics
| I MoriyonSummaryAffiliation: University of Navarra Country: Spain Publications
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Detail Information
Publications
The lipopolysaccharide of Brucella abortus BvrS/BvrR mutants contains lipid A modifications and has higher affinity for bactericidal cationic peptidesLorea Manterola
, Universidad de Navarra, c/ Irunlarrea no. 1, 31008 Pamplona, Spain
J Bacteriol 187:5631-9. 2005..Accordingly, disruption of BvrS/BvrR damages the outer membrane, thus contributing to the severe attenuation manifested by bvrS and bvrR mutants...
Spontaneous Excision of the O-Polysaccharide wbkA Glycosyltranferase Gene Is a Cause of Dissociation of Smooth to Rough Brucella ColoniesMarcos Mancilla
Address correspondence to Ignacio Moriyón
J Bacteriol 194:1860-7. 2012..This ISBm1-mediated wbkA excision and the different %GC levels of the excised fragment and of other wbk genes suggest that the Brucella wbk locus is the result of at least two horizontal acquisition events...
Identification of new IS711 insertion sites in Brucella abortus field isolatesMarcos Mancilla
Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile
BMC Microbiol 11:176. 2011..Thus far, IS711 transposition has only been shown in vitro and only for B. ovis and B. pinnipedialis, two species carrying a high number of IS711 copies, but never in other Brucella species, neither in vitro nor in field strains...
Comparative analysis of selected methods for the assessment of antimicrobial and membrane-permeabilizing activity: a case study for lactoferricin derived peptidesSusana Sanchez-Gomez
Department of Microbiology and Parasitology, University of Navarra, 31080 Pamplona, Spain
BMC Microbiol 8:196. 2008....
DNA polymorphism analysis of Brucella lipopolysaccharide genes reveals marked differences in O-polysaccharide biosynthetic genes between smooth and rough Brucella species and novel species-specific markersMichel S Zygmunt
INRA, UR1282, Infectiologie Animale et Sante Publique, IASP, Nouzilly, France
BMC Microbiol 9:92. 2009..In smooth brucellae, lipopolysaccharide is made of lipid A-core oligosaccharide and N-formylperosamine O-polysaccharide. B. ovis and B. canis (rough species) lack the O-polysaccharide...
Proteomics-based confirmation of protein expression and correction of annotation errors in the Brucella abortus genomeJulie Lamontagne
Caprion Proteomics Inc, 7150 Alexander Fleming, Montreal, Quebec, Canada
BMC Genomics 11:300. 2010..While conducting proteomics studies to better understand Brucella abortus virulence, we consolidated the proteomic data collected and compared it to publically available genomic data...
Structure and properties of the outer membranes of Brucella abortus and Brucella melitensisI Moriyon
Departamento de Microbiologia, Universidad de Navarra, Pamplona, Spain
Int Microbiol 1:19-26. 1998..This model accounts for the permeability of Brucella to hydrophobic permeants and for its resistance to the bactericidal oxygen-independent systems of phagocytes...
Rough vaccines in animal brucellosis: structural and genetic basis and present statusIgnacio Moriyon
Departamento de Microbiologia, Universidad de Navarra, Aptdo 177, 31080 Pamplona, Spain
Vet Res 35:1-38. 2004..Possible uses of rough vaccines also include boosting immunity by revaccination but solid evidence on its effectiveness, safety and practicality is not available...
Regulation of Brucella virulence by the two-component system BvrR/BvrSI Lopez-Goni
Departamento de Microbiologia, Universidad de Navarra, C lrunlarrea no 1, Pamplona 31008, Spain
Vet Microbiol 90:329-39. 2002..We hypothesize that the Brucella BvrR/BvrS is a system devoted to the homeostasis of the outer membrane and, therefore in the interface for cell invasion and mounting the required structures for intracellular parasitism...
Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemoculturesC Gamazo
Departamento de Microbiologia, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain
J Clin Microbiol 31:3200-3. 1993..In NR6 vials buffered to pH 6.2, GVs became positive faster and were higher than those in standard vials. NR6 vials at pH 6.2 with 0.3% sodium pyruvate yielded a positive GV in the first day of bacterial turbidity...
Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and exportDavid Gonzalez
Department of Microbiology and Parasitology, University of Navarra, Pamplona, Spain
PLoS ONE 3:e2760. 2008..Because of this, rough Brucella mutants lacking the O-polysaccharide have been proposed as vaccines...
A two-component regulatory system playing a critical role in plant pathogens and endosymbionts is present in Brucella abortus and controls cell invasion and virulenceA Sola-Landa
Departamento de Microbiologia, Universidad de Navarra, Pamplona, Spain
Mol Microbiol 29:125-38. 1998..As B. abortus, R. meliloti and A. tumefaciens are phylogenetically related, these observations suggest that these systems have a common ancestor that has evolved to sense stimuli in plant and animal microbial environments...
BvrR/BvrS-controlled outer membrane proteins Omp3a and Omp3b are not essential for Brucella abortus virulenceLorea Manterola
Departamento de Microbiologia y Parasitologia, Universidad de Navarra, 31008 Pamplona, Spain
Infect Immun 75:4867-74. 2007..Our results are discussed in the light of previous works suggesting that disruption of omp3a generates attenuated Brucella strains, and we speculate on the role of group 3 Omps...
Genomic island 2 is an unstable genetic element contributing to Brucella lipopolysaccharide spontaneous smooth-to-rough dissociationMarcos Mancilla
Instituto de Bioquimica, Facultad de Ciencias, Universidad Austral de Chile, Valdivia, Chile
J Bacteriol 192:6346-51. 2010..These results suggest that the phage integrase-promoted GI-2 excision played a role in B. ovis speciation and are consistent with other evidence, suggesting that this species and B. canis have emerged as two independent lineages...
Synthesis of phosphatidylcholine, a typical eukaryotic phospholipid, is necessary for full virulence of the intracellular bacterial parasite Brucella abortusRaquel Conde-Alvarez
, Universidad de Navarra, Pamplona, Spain
Cell Microbiol 8:1322-35. 2006..The results suggest that Pcs and PmtA have complementary roles in vivo related to nutrient availability and that PC and the membrane properties that depend on this typical eukaryotic phospholipid are essential for Brucella virulence...
