Alejandro Brun

Summary

Country: Spain

Publications

  1. ncbi [Involvement of the immunological system in the pathogenesis of transmissible spongiform encephalopathies]
    A Brun
    Centro de Investigacion en Sanidad Animal, Nacional de Investigaciones Agrarias, Valdeolmos, Espana
    Rev Neurol 37:648-53. 2003
  2. ncbi Current strategies for subunit and genetic viral veterinary vaccine development
    Alejandro Brun
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, 28130 Madrid, Spain
    Virus Res 157:1-12. 2011
  3. ncbi Antigen delivery systems for veterinary vaccine development. Viral-vector based delivery systems
    Alejandro Brun
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, 28130 Madrid, Spain
    Vaccine 26:6508-28. 2008
  4. ncbi A DNA vaccine encoding ubiquitinated Rift Valley fever virus nucleoprotein provides consistent immunity and protects IFNAR(-/-) mice upon lethal virus challenge
    Hani Boshra
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, Madrid 28130, Spain
    Vaccine 29:4469-75. 2011
  5. ncbi Rift valley fever: recent insights into pathogenesis and prevention
    Hani Boshra
    Centro de Investigación en Sanidad Animal CISA INIA, Carretera de Valdeolmos El Casar s n, Valdeolmos, Madrid, Spain 28130
    J Virol 85:6098-105. 2011
  6. ncbi Comparison of three monoclonal antibodies for use in immunohistochemical detection of bovine spongiform encephalopathy protease-resistant prion protein
    Francisco J Salguero
    Centro de Investigación en Sanidad Animal CISA INIA, Madrid, Spain
    J Vet Diagn Invest 18:106-9. 2006
  7. ncbi Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11
    Alejandro Brun
    , INIA, Ctra Valdeolmos a El Casar sn, Valdeolmos, 28130 Madrid, Spain
    Neurosci Res 48:75-83. 2004
  8. ncbi Subclinical bovine spongiform encephalopathy infection in transgenic mice expressing porcine prion protein
    Joaquin Castilla
    Centro de Investigacion en Sanidad Animal, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Valdeolmos, 28130 Madrid, Spain
    J Neurosci 24:5063-9. 2004
  9. ncbi Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats
    Alejandro Brun
    Centro de Investigacion en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain
    J Gen Virol 88:1842-9. 2007
  10. ncbi Altered lymphocyte homeostasis after oral prion infection in mouse
    F Díaz San Segundo
    Centro de Investigación en Sanidad Animal CISA INIA, Carretera de Algete a El Casar km 8, 100, Valdeolmos 28130, Madrid, Spain
    Vet Immunol Immunopathol 122:204-15. 2008

Collaborators

  • Francisco J Salguero
  • Hani Boshra
  • Esther Blanco
  • Juan Maria Torres
  • Daniel Dory
  • Gunther M Keil
  • Nuria Busquets
  • J Castilla
  • J M Torres
  • Raquel Martín-Folgar
  • Gema Lorenzo
  • B Pintado
  • F Díaz San Segundo
  • B Parra
  • M A Ramirez
  • J M Bilheude
  • F Diaz San Segundo
  • A Rabano
  • A Gutierrez-Adan
  • M J Cano
  • Joaquin Castilla
  • Esther Hevia
  • Belen Borrego
  • Francisco Mateos
  • Javier Iglesias
  • N Sevilla
  • J P Gutierrez
  • N Morel
  • S Lecroix
  • L Gonzalez
  • J Grassi
  • O Andreoletti
  • P Steele
  • J C Espinosa
  • L del Riego
  • Juan M Torres
  • Deirdre Doyle
  • Beatriz Parra
  • Fayna Diaz San Segundo
  • José M Sánchez-Vizcaíno
  • Belen Pintado
  • Alfonso Gutierrez-Adan
  • Mark Rogers
  • Miguel A Ramirez
  • C Sanchez
  • D Doyle
  • J M Sanchez-Vizcaino
  • A Gutierrez Adan
  • M Rogers

Detail Information

Publications19

  1. ncbi [Involvement of the immunological system in the pathogenesis of transmissible spongiform encephalopathies]
    A Brun
    Centro de Investigacion en Sanidad Animal, Nacional de Investigaciones Agrarias, Valdeolmos, Espana
    Rev Neurol 37:648-53. 2003
    ..We review the current knowledge relative to the role of different immune system components and their contribution the spread of prions throughout the infected organism...
  2. ncbi Current strategies for subunit and genetic viral veterinary vaccine development
    Alejandro Brun
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, 28130 Madrid, Spain
    Virus Res 157:1-12. 2011
    ..This paper reviews several antigen production and delivery strategies other than those based on the use of live viral vectors. Genetic and protein subunit vaccines as well as alternative production systems are considered in this review...
  3. ncbi Antigen delivery systems for veterinary vaccine development. Viral-vector based delivery systems
    Alejandro Brun
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, 28130 Madrid, Spain
    Vaccine 26:6508-28. 2008
    ....
  4. ncbi A DNA vaccine encoding ubiquitinated Rift Valley fever virus nucleoprotein provides consistent immunity and protects IFNAR(-/-) mice upon lethal virus challenge
    Hani Boshra
    Centro de Investigación en Sanidad Animal CISA INIA, Valdeolmos, Madrid 28130, Spain
    Vaccine 29:4469-75. 2011
    ....
  5. ncbi Rift valley fever: recent insights into pathogenesis and prevention
    Hani Boshra
    Centro de Investigación en Sanidad Animal CISA INIA, Carretera de Valdeolmos El Casar s n, Valdeolmos, Madrid, Spain 28130
    J Virol 85:6098-105. 2011
    ..Progress in understanding the epidemiology of Rift Valley fever has also enabled prediction of potential outbreaks well in advance, thus providing another tool to combat the physical and economic impact of this disease...
  6. ncbi Comparison of three monoclonal antibodies for use in immunohistochemical detection of bovine spongiform encephalopathy protease-resistant prion protein
    Francisco J Salguero
    Centro de Investigación en Sanidad Animal CISA INIA, Madrid, Spain
    J Vet Diagn Invest 18:106-9. 2006
    ..In fact, 1 MAb (2A11) was able to react with PrPres deposits in the absence of a denaturing step, yielding the strongest signal and confirming the usefulness of MAb 2A11 in immunohistochemistry for the diagnosis of BSE...
  7. ncbi Proteinase K enhanced immunoreactivity of the prion protein-specific monoclonal antibody 2A11
    Alejandro Brun
    , INIA, Ctra Valdeolmos a El Casar sn, Valdeolmos, 28130 Madrid, Spain
    Neurosci Res 48:75-83. 2004
    ..Since definitive diagnosis of prion diseases rely on IHC assays of proteinase K-treated samples, the use of mAb 2A11 might contribute to reduce the occurrence of false positive detection due to incomplete proteinase K digestion...
  8. ncbi Subclinical bovine spongiform encephalopathy infection in transgenic mice expressing porcine prion protein
    Joaquin Castilla
    Centro de Investigacion en Sanidad Animal, Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Valdeolmos, 28130 Madrid, Spain
    J Neurosci 24:5063-9. 2004
    ..Collectively, our findings suggest that these poTg mice could be used as a sensitive bioassay model for prion detection in pigs...
  9. ncbi Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats
    Alejandro Brun
    Centro de Investigacion en Sanidad Animal, INIA, Valdeolmos, 28130 Madrid, Spain
    J Gen Virol 88:1842-9. 2007
    ..These results raise the possibility of selecting cattle breeds bearing the 5OR Prnp allele that are less susceptible to prion infection...
  10. ncbi Altered lymphocyte homeostasis after oral prion infection in mouse
    F Díaz San Segundo
    Centro de Investigación en Sanidad Animal CISA INIA, Carretera de Algete a El Casar km 8, 100, Valdeolmos 28130, Madrid, Spain
    Vet Immunol Immunopathol 122:204-15. 2008
    ..Altogether, these results suggest that normal regulation of lymphocyte populations becomes altered along the progression of a prion infection...
  11. ncbi Development and characterization of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein generated by DNA immunization
    Raquel Martín-Folgar
    Centro de Investigacion en Sanidad Animal, Carretera de Valdeolmos, Valdeolmos, Madrid, Spain
    MAbs 2:275-84. 2010
    ..These monoclonal antibodies constitute useful tools for diagnosis, especially for the detection of serum anti-RVFV antibodies from a broad range of species by means of competitive ELISA...
  12. ncbi Protection against lethal Rift Valley fever virus (RVFV) infection in transgenic IFNAR(-/-) mice induced by different DNA vaccination regimens
    Gema Lorenzo
    Centro de Investigación en Sanidad Animal CISA INIA, Ctra Algete El Casar, 28130 Valdeolmos, Madrid, Spain
    Vaccine 28:2937-44. 2010
    ..Interestingly, the combination of both vaccine constructs induced specific lymphoblast proliferation upon stimulation with a recombinant nucleoprotein...
  13. ncbi Transgenic mice expressing bovine PrP with a four extra repeat octapeptide insert mutation show a spontaneous, non-transmissible, neurodegenerative disease and an expedited course of BSE infection
    J Castilla
    Centro de Investigacion en Sanidad Animal, INIA, 28130 Valdeolmos, Madrid, Spain
    FEBS Lett 579:6237-46. 2005
    ..This Tg mouse model constitutes a new way of understanding the pathobiology of bovine transmissible spongiform encephalopathy. Its potential applications include the assessment of new therapies against prion diseases...
  14. ncbi Vertical transmission of bovine spongiform encephalopathy prions evaluated in a transgenic mouse model
    J Castilla
    , Ctra. de Valdeolmes a El Casar, Valdeolmos, 28130 Madrid, Spain
    J Virol 79:8665-8. 2005
    ..Also, these results may complement previous epidemiological data supporting the occurrence of vertical BSE transmission in cattle...
  15. ncbi Different behavior toward bovine spongiform encephalopathy infection of bovine prion protein transgenic mice with one extra repeat octapeptide insert mutation
    J Castilla
    Center of Animal Health Investigation, National Institute of Agricultural Technology and Investigation, Valdeolmos, 28130 Madrid, Spain
    J Neurosci 24:2156-64. 2004
    ..These findings may help us improve the current mouse bioassays and understand the role of the octapeptide repeat region in susceptibility to disease...
  16. ncbi Early detection of PrPres in BSE-infected bovine PrP transgenic mice
    J Castilla
    Centro de Investigacion en Sanidad Animal, INIA, Madrid, Spain
    Arch Virol 148:677-91. 2003
    ..Interestingly, transgenic mice-propagated sheep scrapie prions displayed distinct biochemical properties when compared to both the original sheep scrapie and transgenic mouse-propagated BSE inoculum...
  17. ncbi [Transmissible spongiform encephalopathies in animals]
    A Brun
    Instituto Nacional de Investigaciones Agrarias INIA, Valdeolmos, Madrid, Espana
    Rev Neurol 31:133-7. 2000
    ..Amongst these, undoubtedly the best known is that affecting cattle, since the association between consumption of beef and its derivatives and the appearance of a variant of Creutzfeldt-Jakob disease in humans has been established...
  18. ncbi Discrimination of sheep susceptible and resistant to transmissible spongiform encephalopathies by an haplotype specific monoclonal antibody
    J M Bilheude
    Bio Rad, R and D TSE, 3 Bd Raymond Poincaré, 92430 Marnes La Coquette, France
    J Virol Methods 145:169-72. 2007
    ..The test allows the efficient discrimination of R(171) homozygotes, R(171) heterozygotes and non-R(171) carriers, therefore offering a rapid, cheap and easy to use alternative method to select sheep for their resistance to scrapie...