Research Topics
Species | Lyn Marie BirkholtzSummaryAffiliation: University of Pretoria Country: South Africa Publications
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Detail Information
Publications
Polyamine homoeostasis as a drug target in pathogenic protozoa: peculiarities and possibilitiesLyn Marie Birkholtz
Department of Biochemistry, University of Pretoria, Hatfield, South Africa
Biochem J 438:229-44. 2011..Particularly interesting features within polyamine metabolism in these parasites are highlighted for their potential in selective therapeutic strategies...
Heterologous expression of plasmodial proteins for structural studies and functional annotationLyn Marie Birkholtz
Department of Biochemistry, University of Pretoria, Pretoria, South Africa
Malar J 7:197. 2008..Here the status quo of heterologous production of plasmodial proteins is presented, constraints are highlighted and alternative strategies and hosts for functional expression and annotation of plasmodial proteins are reviewed...
Novel properties of malarial S-adenosylmethionine decarboxylase as revealed by structural modellingGordon A Wells
Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa
J Mol Graph Model 24:307-18. 2006..These novel features of malarial AdoMetDC suggest possibilities for the discovery of parasite-specific inhibitors...
Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levelsJohn V W Becker
CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa
BMC Genomics 11:235. 2010..falciparum spermidine synthase (PfSpdSyn) were examined on a morphological, transcriptomic, proteomic and metabolic level...
Biochemical characterisation and novel classification of monofunctional S-adenosylmethionine decarboxylase of Plasmodium falciparumMarni Williams
Department of Biochemistry, University of Pretoria, Hatfield, South Africa
Mol Biochem Parasitol 180:17-26. 2011..Furthermore, based on the results obtained in this study we propose a new AdoMetDC subclass for plasmodial AdoMetDCs...
Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative studyMarni Williams
Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa
Malar J 6:64. 2007..A comparative study of five widely used PCR-based mutagenesis methods identified a modified inverse PCR method as particularly suitable for the deletion of large areas (>100 bp) in malaria parasite genes...
Transcriptional responses of Plasmodium falciparum to alpha-difluoromethylornithine-induced polyamine depletionKatherine Clark
Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa
Biol Chem 389:111-25. 2008..The data presented provide input into the role of the polyamines in the cellular biology of P. falciparum and contribute towards the validation of polyamine biosynthesis as an antimalarial target...
Proteomic profiling of Plasmodium falciparum through improved, semiquantitative two-dimensional gel electrophoresisSalome Smit
Department of Biochemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, 0002, South Africa
J Proteome Res 9:2170-81. 2010....
Discovery of novel alkylated (bis)urea and (bis)thiourea polyamine analogues with potent antimalarial activitiesBianca K Verlinden
Department of Biochemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, PO Box x20, Pretoria, 0028, South Africa
J Med Chem 54:6624-33. 2011....
Integration and mining of malaria molecular, functional and pharmacological data: how far are we from a chemogenomic knowledge space?Lyn Marie Birkholtz
Department of Biochemistry and African Centre for Gene Technologies, Faculty of Natural and Agricultural Sciences, University of Pretoria, 0002, Pretoria, South Africa
Malar J 5:110. 2006..Recent progress towards a grid-enabled chemogenomic knowledge space is discussed...
Co-inhibition of Plasmodium falciparum S-adenosylmethionine decarboxylase/ornithine decarboxylase reveals perturbation-specific compensatory mechanisms by transcriptome, proteome, and metabolome analysesAnna C van Brummelen
Department of Biochemistry, University of Pretoria, Pretoria, Gauteng 0002, South Africa
J Biol Chem 284:4635-46. 2009..In contrast with previous reports, the results provide evidence that P. falciparum responds to alleviate the detrimental effects of polyamine depletion via regulation of its transcriptome and subsequently the proteome and metabolome...
Structural and mechanistic insights into the action of Plasmodium falciparum spermidine synthasePieter B Burger
Bioinformatics and Computational Biology Unit, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria 0002, South Africa
Bioorg Med Chem 15:1628-37. 2007....
