Darren J Schofield

Summary

Publications

  1. ncbi Application of phage display to high throughput antibody generation and characterization
    Darren J Schofield
    Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK
    Genome Biol 8:R254. 2007
  2. ncbi Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine
    Darren J Schofield
    Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Vaccine 22:257-67. 2003
  3. ncbi Identification of chimpanzee Fab fragments by repertoire cloning and production of a full-length humanized immunoglobulin G1 antibody that is highly efficient for neutralization of dengue type 4 virus
    Ruhe Men
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 78:4665-74. 2004
  4. ncbi Human monoclonal antibodies that react with the E2 glycoprotein of hepatitis C virus and possess neutralizing activity
    Darren J Schofield
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892-8009, USA
    Hepatology 42:1055-62. 2005
  5. ncbi A simple vector system to improve performance and utilisation of recombinant antibodies
    Cecile D Martin
    Atlas of Protein Expression, Wellcome Trust Sanger Institute, Genome Campus, Morgan Building, Hinxton, Cambridgeshire, CB10 1HH, UK
    BMC Biotechnol 6:46. 2006

Collaborators

  • Susan Dj Chapple
  • R H Purcell
  • Cecile D Martin
  • Ruhe Men
  • Gertrudis Rojas
  • John McCafferty
  • Jiahua Wu
  • Joanne N Mitchell
  • Karen J Vincent
  • Claire Wernly
  • Ching-Juh Lai
  • Ana P Goncalvez
  • Tetsu Yamashiro
  • Suzanne U Emerson

Detail Information

Publications5

  1. ncbi Application of phage display to high throughput antibody generation and characterization
    Darren J Schofield
    Abcam Ltd, Cambridge Science Park, Cambridge CB4 0FW, UK
    Genome Biol 8:R254. 2007
    ..Thus, we have demonstrated the potential of and illuminated the issues associated with genome-wide monoclonal antibody generation...
  2. ncbi Monoclonal antibodies that neutralize HEV recognize an antigenic site at the carboxyterminus of an ORF2 protein vaccine
    Darren J Schofield
    Hepatitis Viruses and Molecular Hepatitis Sections, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA
    Vaccine 22:257-67. 2003
    ....
  3. ncbi Identification of chimpanzee Fab fragments by repertoire cloning and production of a full-length humanized immunoglobulin G1 antibody that is highly efficient for neutralization of dengue type 4 virus
    Ruhe Men
    Molecular Viral Biology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 78:4665-74. 2004
    ..03 to 0.05 micro g/ml. The DENV-4 binding affinities were 0.42 nM for Fab 5H2 and 0.24 nM for full-length IgG1 5H2. Monoclonal antibody IgG1 5H2 may prove valuable for passive immunoprophylaxis against dengue virus in humans...
  4. ncbi Human monoclonal antibodies that react with the E2 glycoprotein of hepatitis C virus and possess neutralizing activity
    Darren J Schofield
    Hepatitis Viruses Section, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892-8009, USA
    Hepatology 42:1055-62. 2005
    ..In conclusion, one or more of these monoclonal antibodies may be useful in preventing infections by HCV belonging to genotype 1 or 2, the most medically important genotypes worldwide...
  5. ncbi A simple vector system to improve performance and utilisation of recombinant antibodies
    Cecile D Martin
    Atlas of Protein Expression, Wellcome Trust Sanger Institute, Genome Campus, Morgan Building, Hinxton, Cambridgeshire, CB10 1HH, UK
    BMC Biotechnol 6:46. 2006
    ..However, they are often inefficient for expression of soluble antibody fragments, and sub-cloning of selected antibody populations into dedicated soluble antibody fragment expression vectors can enhance expression...