Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometryRangarajan Sampath
Ibis Biosciences Inc, Subsidiary of Isis Pharmaceuticals, Carlsbad, CA 92008, USA
Ann N Y Acad Sci 1102:109-20. 2007
..It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens...
- Rapid identification of emerging pathogens: coronavirus
Rangarajan Sampath
Ibis Therapeutics, 1891 Rutherford Ct, Carlsbad, CA 92008, USA
Emerg Infect Dis 11:373-9. 2005
..This approach, applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens, is capable of automated analysis of >900 PCR reactions per day...
- Global surveillance of emerging Influenza virus genotypes by mass spectrometry
Rangarajan Sampath
Ibis Biosciences Inc, A Wholly Owned Subsidiary of Isis Pharmaceuticals, Carlsbad, California, United States of America
PLoS ONE 2:e489. 2007
..We have developed one such approach based on previously described broad-range reverse transcription PCR/electrospray ionization mass spectrometry (RT-PCR/ESI-MS) technology...
- Molecular genotyping of microbes by multilocus PCR and mass spectrometry: a new tool for hospital infection control and public health surveillance
David J Ecker
Ibis Biosciences, 1896 Rutherford Rd, Carlsbad, CA, USA
Methods Mol Biol 551:71-87. 2009
..This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable...
- Rapid molecular genotyping and clonal complex assignment of Staphylococcus aureus isolates by PCR coupled to electrospray ionization-mass spectrometry
Thomas A Hall
Ibis Biosciences, Inc, 1896 Rutherford Rd, Carlsbad, California 92008, USA
J Clin Microbiol 47:1733-41. 2009
..The PCR/ESI-MS method enables genotyping of over 180 samples of S. aureus per day in an automated fashion...
- High-resolution genotyping of Campylobacter species by use of PCR and high-throughput mass spectrometry
James C Hannis
Ibis Biosciences, Inc, 1891 Rutherford Road, Carlsbad, CA 92008, USA
J Clin Microbiol 46:1220-5. 2008
..jejuni and 25 strain types of C. coli are presented. These samples were parsed and resolved with demonstrated sensitivity down to 10 genomes/PCR from pure isolates...
- New technology for rapid molecular diagnosis of bloodstream infections
David J Ecker
Ibis Biosciences, a subsidiary of Abbott Molecular, Inc, 2251 Faraday Ave, Carlsbad, CA 92008, USA
Expert Rev Mol Diagn 10:399-415. 2010
..We then focus on a new method that combines nucleic acid amplification with mass spectrometry in a novel approach to molecular diagnosis of bloodstream infections...
- The Microbial Rosetta Stone database: A common structure for microbial biosecurity threat agents
David J Ecke
Ibis Division of Isis Pharmaceuticals, 1891 Rutherford Rd, Carlsbad, CA 92008, USA
J Forensic Sci 50:1380-5. 2005
..microbialrosettastone.com. This website provides a single location where access to information on a broad range of disease-causing organisms and toxins is available to members of the biosecurity community...
- Identification of Acinetobacter species and genotyping of Acinetobacter baumannii by multilocus PCR and mass spectrometry
Joseph A Ecker
Ibis Biosciences, Isis Pharmaceuticals, Inc, 1891 Rutherford Road, Carlsbad, CA 92008, USA
J Clin Microbiol 44:2921-32. 2006
..This rapid analysis allows tracking of transmission for the implementation of appropriate infection control measures on a time scale previously not achievable...
- Direct broad-range detection of alphaviruses in mosquito extracts
Mark W Eshoo
Ibis Biosciences, 1891 Rutherford Rd, Carlsbad, CA 92008, USA
Virology 368:286-95. 2007
..The assay we have developed provides a rapid, accurate, and high-throughput assay for surveillance of alphaviruses...
- Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance
David J Ecker
Ibis Division of Isis Pharmaceuticals, 1891 Rutherford Road, Carlsbad, CA 92008, USA
Proc Natl Acad Sci U S A 102:8012-7. 2005
..This analysis method will provide information fundamental to understanding the polymicrobial nature of explosive epidemics of respiratory disease...
- Rapid and high-throughput pan-Orthopoxvirus detection and identification using PCR and mass spectrometry
Mark W Eshoo
Ibis Biosciences, Carlsbad, CA, USA
PLoS ONE 4:e6342. 2009
..The assay is sensitive at the stochastic limit of PCR and detected virus in blood containing approximately six plaque-forming units per milliliter from a rabbitpox virus-infected rabbit...
- Ibis T5000: a universal biosensor approach for microbiology
David J Ecker
Ibis Biosciences, 1896 Rutherford Road, Carlsbad, California 92008, USA
Nat Rev Microbiol 6:553-8. 2008
....
- Simultaneous identification of mycobacterial isolates to the species level and determination of tuberculosis drug resistance by PCR followed by electrospray ionization mass spectrometry
Christian Massire
Ibis Biosciences, Inc, 2251 Faraday Ave, Carlsbad, CA 92008, USA
J Clin Microbiol 49:908-17. 2011
..The assay also provides information on ethambutol, fluoroquinolone, and diarylquinoline resistance and lineage-specific polymorphisms, to yield highly discriminative digital signatures potentially suitable for epidemiology tracking...
- Rapid detection and molecular serotyping of adenovirus by use of PCR followed by electrospray ionization mass spectrometry
Lawrence B Blyn
Ibis Biosciences, Inc, Carlsbad, CA 92008, USA
J Clin Microbiol 46:644-51. 2008
..Over 500 samples per day can be analyzed with sensitivities greater than 100 genomes per reaction. This approach can be applied to many other families of infectious agents for rapid and sensitive analysis...
- Base composition analysis of human mitochondrial DNA using electrospray ionization mass spectrometry: a novel tool for the identification and differentiation of humans
Thomas A Hall
Ibis Therapeutics, A Division of Isis Pharmaceuticals, 1891 Rutherford Road, Carlsbad, CA 92008, USA
Anal Biochem 344:53-69. 2005
..This assay can be expanded to assess genetic variation in the coding region of the mtDNA genome and can be automated to facilitate analysis of a large number of samples such as those encountered after a mass disaster...
- The Microbial Rosetta Stone Database: a compilation of global and emerging infectious microorganisms and bioterrorist threat agents
David J Ecker
Ibis Therapeutics, 1891 Rutherford Rd, Carlsbad, CA 92008, USA
BMC Microbiol 5:19. 2005
..This ambiguity can be a significant block to effective communication among the diverse communities that must deal with epidemics or bioterrorist attacks...
- SAR by MS: discovery of a new class of RNA-binding small molecules for the hepatitis C virus: internal ribosome entry site IIA subdomain
Punit P Seth
Ibis Therapeutics, A Division of Isis Pharmaceuticals Inc, 1891 Rutherford Road, Carlsbad, California 92008, USA
J Med Chem 48:7099-102. 2005
..The optimized benzimidazoles demonstrated activity in a cellular replicon assay at concentrations comparable to their KD for the RNA target...
- Detection and identification of Ehrlichia species in blood by use of PCR and electrospray ionization mass spectrometry
Mark W Eshoo
Ibis Biosciences, Carlsbad, California 92008, USA
J Clin Microbiol 48:472-8. 2010
..Moreover, this system may provide rapid detection and identification of additional pathogens directly from blood specimens...
- Identification of conserved regulatory RNA structures in prokaryotic metabolic pathway genes
Elena A Lesnik
Ibis Therapeutics, Carlsbad, CA 92008, USA
Biosystems 80:145-54. 2005
..The results demonstrate that this computational approach is a powerful tool for discovery of important RNA structures within prokaryotic organisms...
- A bioinformatics based approach to discover small RNA genes in the Escherichia coli genome
Shuo Chen
Ibis Therapeutics, Isis Pharmaceuticals, Inc, 2292 Faraday Ave, Carlsbad, CA 92008, USA
Biosystems 65:157-77. 2002
..Here we summarize our data and discuss the rules and advantages/disadvantages of using this approach in annotating sRNA genes on bacterial genomes...
- Genotypic evolution of Acinetobacter baumannii strains in an outbreak associated with war trauma
Glenn Wortmann
Infectious Disease Service, Walter Reed Army Medical Center, Washington, DC 20307 5001, USA
Infect Control Hosp Epidemiol 29:553-5. 2008
..Using multilocus polymerase chain reaction (PCR) and mass spectrometry to genotype isolates, we found an atypical and evolving strain distribution, distinct from those found at nonmilitary hospitals in the United States...
