Research Topics
Genomes and Genes | Jozef DulakSummaryAffiliation: Jagiellonian University Country: Poland Publications
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Publications
Opposite effects of HIF-1? and HIF-2? on the regulation of IL-8 expression in endothelial cellsUrszula Florczyk
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, 30 387 Krakow, Poland
Free Radic Biol Med 51:1882-92. 2011..However, despite stabilization of both isoforms in hypoxia the effect of HIF-1 is predominant, and downregulation of IL-8 expression in hypoxia is caused by attenuation of Nrf2 and c-Myc...
Stem cells in pharmaceutical biotechnologyEwa K Zuba-Surma
Department of Medical Biotechnology, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Curr Pharm Biotechnol 12:1760-73. 2011..All together, the applications of various cell types derived from patient specific pluripotent stem cells may lead to targeted drug and cellular therapies for certain individuals...
Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic miceAgnieszka Jazwa
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Genet Vaccines Ther 8:6. 2010....
Heme oxygenase-1 and the vascular bed: from molecular mechanisms to therapeutic opportunitiesAgnieszka Loboda
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Antioxid Redox Signal 10:1767-812. 2008....
15d-PGJ2 upregulates synthesis of IL-8 in endothelial cells through induction of oxidative stressAlicja Jozkowicz
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
Antioxid Redox Signal 10:2035-46. 2008..In summary, we showed that 15d-PGJ(2), acting through oxidative stress, may exert proinflammatory effects. The upregulation of IL-8 is mostly associated with p38-mediated stabilization of mRNA...
Atorvastatin prevents hypoxia-induced inhibition of endothelial nitric oxide synthase expression but does not affect heme oxygenase-1 in human microvascular endothelial cellsAgnieszka Loboda
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Atherosclerosis 187:26-30. 2006..Therefore, we suggest that the protective effect of statins at their pharmacological concentrations is not mediated by enhancement of HO-1 activity, but may involve eNOS...
HIF-1 induction attenuates Nrf2-dependent IL-8 expression in human endothelial cellsAgnieszka Loboda
Department of Medical Biotechnology, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Antioxid Redox Signal 11:1501-17. 2009..However, expression of IL-8 is independent of HO-1. Cross-talk between HIF-1 and Nrf2 may influence the outcome of anti-angiogenic therapies aimed at targeting HIF-1. Antioxid...
HIF-1 attenuates Ref-1 expression in endothelial cells: reversal by siRNA and inhibition of geranylgeranylationAgnieszka Loboda
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Vascul Pharmacol 51:133-9. 2009..The regulation of Ref-1 by statins may be considered as a novel mechanism of their beneficial effects on endothelium...
Angiogenic transcriptome of human microvascular endothelial cells: Effect of hypoxia, modulation by atorvastatinAgnieszka Loboda
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland
Vascul Pharmacol 44:206-14. 2006..The influence of atorvastatin on angiogenic gene expression is complex, and final pro- or anti-angiogenic outcome of statin therapy remains to be established for numerous angiogenesis-related diseases...
Heme oxygenase-1 accelerates cutaneous wound healing in miceAnna Grochot-Przeczek
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
PLoS ONE 4:e5803. 2009..In summary, induction of HO-1 is necessary for efficient wound closure and neovascularization. Impaired wound healing in diabetic mice may be associated with delayed HO-1 upregulation and can be improved by HO-1 gene transfer...
Biliverdin reductase: new features of an old enzyme and its potential therapeutic significanceUrszula M Florczyk
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, Krakow, Poland
Pharmacol Rep 60:38-48. 2008..These features make BVR unusually interesting and unique among all enzymes characterized to date...
Role of heme oxygenase-1 in hydrogen peroxide-induced VEGF synthesis: effect of HO-1 knockoutJarosław Cisowski
Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Biochem Biophys Res Commun 326:670-6. 2005..Additionally, knock out of HO-1 gene impaired induction of VEGF by hemin, lysophosphatidylcholine, and prostaglandin-J(2). Our results provide confirmation for the involvement of HO-1 in regulation of angiogenesis...
Effect of heme and heme oxygenase-1 on vascular endothelial growth factor synthesis and angiogenic potency of human keratinocytesAgnieszka Jazwa
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Free Radic Biol Med 40:1250-63. 2006..Recently the involvement of HO-1 in angiogenesis has been shown; however, the role of heme and HO-1 in wound healing angiogenesis has not been yet investigated...
Atorvastatin affects several angiogenic mediators in human endothelial cellsJozef Dulak
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Endothelium 12:233-41. 2005..In conclusion, the present findings points to other angiogenesis-related effects of atorvastatin, which may be of relevance to the beneficial influence of statins in cardiovascular system...
Aristolochic acid I and ochratoxin A differentially regulate VEGF expression in porcine kidney epithelial cells--the involvement of SP-1 and HIFs transcription factorsAnna Stachurska
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Toxicol Lett 204:118-26. 2011..These observations provide new insight into complex impact of AAI and OTA on angiogenic gene regulation. Additionally, it adds to our understanding of hypoxia influence on nephropathies pathology...
Heme oxygenase-1 in tumor biology and therapyHalina Was
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Curr Drug Targets 11:1551-70. 2010..Finally, it suggests that pharmacological agents that regulate HO activity or HO-1 gene silencing may become powerful tools for preventing the onset or progression of various cancers and sensitize them to anticancer therapies...
Heme oxygenase-1 in neovascularisation: A diabetic perspectiveAnna Grochot-Przeczek
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Thromb Haemost 104:424-31. 2010..This review focuses on the role of HO-1 in angiogenesis and vasculogenesis, having a closer look at the significance of this system in diabetes...
Effects of heme oxygenase-1 on induction and development of chemically induced squamous cell carcinoma in miceHalina Was
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Free Radic Biol Med 51:1717-26. 2011..Thus, HO-1 may protect healthy tissues against carcinogen-induced injury, but in already growing tumors it seems to favor their progression toward more malignant forms...
Overexpression of biliverdin reductase enhances resistance to chemotherapeuticsUrszula Florczyk
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Cancer Lett 300:40-7. 2011..Therefore activity of BVR may be of significance in tumors and may influence the effectiveness of therapies...
Haem oxygenase-1: non-canonical roles in physiology and pathologyAnna Grochot-Przeczek
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Clin Sci (Lond) 122:93-103. 2012....
New vectors and strategies for cardiovascular gene therapyAgnieszka Jazwa
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Curr Gene Ther 7:7-23. 2007....
New strategies for cardiovascular gene therapy: regulatable pre-emptive expression of pro-angiogenic and antioxidant genesJozef Dulak
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Cell Biochem Biophys 44:31-42. 2006..Here, we demonstrated some indications of these drawbacks. Therefore, the final acceptance of these promising strategies for clinical trials requires careful validation in animal experiments...
Heme oxygenase-1 and carbon monoxide in vascular pathobiology: focus on angiogenesisJozef Dulak
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
Circulation 117:231-41. 2008..This review will provide an overview of the role of heme oxygenase-1 and carbon monoxide in angiogenesis...
Heme oxygenase-1 inhibits myoblast differentiation by targeting myomirsMagdalena Kozakowska
Department of Medical Biotechnology, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Antioxid Redox Signal 16:113-27. 2012..Its overexpression has been suggested as a strategy improving survival of transplanted muscle precursors...
CD45-CD14 +CD34 + murine bone marrow low-adherent mesenchymal primitive cells preserve multilineage differentiation potential in long-term in vitro cultureKrzysztof Szade
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Mol Cells 31:497-507. 2011..In sum, we isolated the novel multipotent subpopulation of CD45(-)CD14(+) CD34(+) bone marrow-derived cells that could be maintained in long-term culture without losing this potential...
Gene transfer of CuZn superoxide dismutase enhances the synthesis of vascular endothelial growth factorJolanta Grzenkowicz-Wydra
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Mol Cell Biochem 264:169-81. 2004..This data suggest the feasibility of stimulation of angiogenesis by overexpression of SOD1...
Overexpression of heme oxygenase-1 in murine melanoma: increased proliferation and viability of tumor cells, decreased survival of miceHalina Was
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Am J Pathol 169:2181-98. 2006....
HIF-1 and HIF-2 transcription factors--similar but not identicalAgnieszka Loboda
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Mol Cells 29:435-42. 2010..Finally, recent data showing link between HIFs and specific microRNA have been presented...
Heme oxygenase-1 in tumors: is it a false friend?Alicja Jozkowicz
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland
Antioxid Redox Signal 9:2099-117. 2007..Accordingly, inhibition of HO-1 can be suggested as a potential therapeutic approach sensitizing tumors to radiation, chemotherapy, or photodynamic therapy...
MicroRNAs as biomarkers of disease onsetMaciej Ciesla
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Anal Bioanal Chem 401:2051-61. 2011..Together with relative stability, specificity, and reproducibility, they are seen as good candidates for early recognition of the onset of disease. Thus, miRNAs might be considered as biomarkers for many pathological states...
Effect of heme oxygenase-1 on vascular function and diseaseJozef Dulak
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Curr Opin Lipidol 19:505-12. 2008..The present review summarizes the most recent studies about heme oxygenase-1 involvement in atherosclerosis, neovascularization and endothelial progenitor cells biology...
Oxidative stress in tumor angiogenesis- therapeutic targetsMagdalena Tertil
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Curr Pharm Des 16:3877-94. 2010....
Helper-dependent adenoviral vectors in experimental gene therapyAlicja Jozkowicz
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Acta Biochim Pol 52:589-99. 2005..In this review we characterize the basic features of HdAd vectors and describe some of their experimental and potential clinical applications...
Heme oxygenase activity modulates vascular endothelial growth factor synthesis in vascular smooth muscle cellsJozef Dulak
Division of Cardiology, Innsbruck University, Innsbruck, Austria
Antioxid Redox Signal 4:229-40. 2002..As CO and iron, respectively the inducer and the inhibitor of VEGF synthesis, are concomitantly produced during the degradation of heme, these data indicate that HO by-products may differentially modulate VEGF production...
Nitric oxide-dependent synthesis of vascular endothelial growth factor is impaired by high glucoseJozef Dulak
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Life Sci 75:2573-86. 2004..This may play a role in the development and progression of vascular dysfunctions in cardiovascular diseases...
Anti-angiogenic and anti-inflammatory effects of statins: relevance to anti-cancer therapyJozef Dulak
Department of Medical Biotechnology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Curr Cancer Drug Targets 5:579-94. 2005..Herein, the reasons for those outcomes are discussed and the rationale for further studies is presented...
HIF-1: the knowns and unknowns of hypoxia sensingAnna Zagorska
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland
Acta Biochim Pol 51:563-85. 2004..Further understanding of the processes that control HIF-1 activity will be crucial for the development of new diagnostic and therapeutic strategies...
Complex role of heme oxygenase-1 in angiogenesisJozef Dulak
Department of Cell Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30 387 Krakow, Poland
Antioxid Redox Signal 6:858-66. 2004....
