Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probeB Ou
Brunswick Laboratories, 6 Thacher Lane, Wareham, Massachusetts 02571, USA
J Agric Food Chem 49:4619-26. 2001
..It is concluded that, unlike other popular methods, the improved ORAC(FL) assay provides a direct measure of hydrophilic chain-breaking antioxidant capacity against peroxyl radical...
- Analysis of antioxidant activities of common vegetables employing oxygen radical absorbance capacity (ORAC) and ferric reducing antioxidant power (FRAP) assays: a comparative study
Boxin Ou
Brunswick Laboratories, 6 Thacher Lane, Wareham, Massachusetts 02571, USA
J Agric Food Chem 50:3122-8. 2002
..On the basis of the ORAC results, green pepper, spinach, purple onion, broccoli, beet, and cauliflower are the leading sources of antioxidant activities against the peroxyl radicals...
- When east meets west: the relationship between yin-yang and antioxidation-oxidation
Boxin Ou
Brunswick Laboratories, Wareham, Massachusetts 02571, USA
FASEB J 17:127-9. 2003
..Our hypothesis opens an avenue to systematically study the yin-yang balance and its health implications with the use of modern biochemical tools...
- The chemistry behind antioxidant capacity assays
Dejian Huang
Food Science and Technology Program, Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
J Agric Food Chem 53:1841-56. 2005
..To comprehensively study different aspects of antioxidants, validated and specific assays are needed in addition to these two commonly accepted assays...
- High-throughput quantitation of peroxyl radical scavenging capacity in bulk oils
Kai Xin Hay
Food Science and Technology Programme, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
J Agric Food Chem 54:5299-305. 2006
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- Phytochemical and nutrient composition of the freeze-dried amazonian palm berry, Euterpe oleraceae mart. (acai)
Alexander G Schauss
Natural and Medicinal Products Research, AIMBR Life Sciences, 4117 South Meridian, Puyallup, Washington 98373, USA
J Agric Food Chem 54:8598-603. 2006
..The total sterols accounted for 0.048% by weight of powder. The three sterols B-sitosterol, campesterol, and sigmasterol were identified. A complete nutrient analysis is also presented. Microbiological analysis was also performed...
- Antioxidant capacity and other bioactivities of the freeze-dried Amazonian palm berry, Euterpe oleraceae mart. (acai)
Alexander G Schauss
Natural and Medicinal Products Research, AIMBR Life Sciences, 4117 South Meridian, Puyallup, Washington 98373, USA
J Agric Food Chem 54:8604-10. 2006
..Acai was found to be a potential cyclooxygenase (COX)-1 and COX-2 inhibitor. It also showed a weak effect on lipopolysaccharide (LPS)-induced nitric oxide but no effect on either lymphocyte proliferation and phagocytic capacity...
- Procyanidin and catechin contents and antioxidant capacity of cocoa and chocolate products
Liwei Gu
Arkansas Children's Nutrition Center, ARS-USDA, and Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72202, USA
J Agric Food Chem 54:4057-61. 2006
..These results indicate that NFCS is correlated with AOC and PC in cocoa and chocolate products. Alkalizing dramatically decreased both the procyanidin content and antioxidant capacity, although not to the same extent...
- Fluorescent approach to quantitation of reactive oxygen species in mainstream cigarette smoke
Boxin Ou
Brunswick Laboratories, LLC, 6 Thatcher Ln, Wareham, Massachusetts 02751, USA
Anal Chem 78:3097-103. 2006
..The smokeless cigarette, Eclipse, has comparable ROS with cigarettes made of bright tobacco...
- Antioxidant activity and polyphenol and procyanidin contents of selected commercially available cocoa-containing and chocolate products in the United States
Kenneth B Miller
The Hershey Company, P.O. Box 805, Hershey, Pennsylvania 17033-0805, USA
J Agric Food Chem 54:4062-8. 2006
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- Chemical composition of caneberry (Rubus spp.) seeds and oils and their antioxidant potential
B Shaun Bushman
Forage and Range Research Laboratory, USDA ARS, Utah State University, 695 N 1100 E, Logan, Utah 84322 6300, USA
J Agric Food Chem 52:7982-7. 2004
..Ellagitannins and free ellagic acid were the main phenolics detected in all five caneberry species and were approximately 3-fold more abundant in the blackberries and the boysenberry than in the raspberries...
- Anthocyanin and proanthocyanidin content in selected white and red wines. Oxygen radical absorbance capacity comparison with nontraditional wines obtained from highbush blueberry
Concepción Sánchez-Moreno
Nutrition and Neurocognitive Laboratory, Jean Mayer Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, Massachusetts 02111, USA
J Agric Food Chem 51:4889-96. 2003
..04 +/- 0.81 mmol of TE, 0.47 +/- 0.15 mmol of TE, and 2.42 +/- 0.88 mmol of TE of ORAC(PE)/day, respectively...
- Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC(FL))) of plasma and other biological and food samples
Ronald L Prior
U S Department of Agriculture, Agriculture Research Service, Arkansas, Children s Nutrition Center, 1120 Marshall Street, Little Rock, Arkansas 72202, USA
J Agric Food Chem 51:3273-9. 2003
..9% of the total antioxidant capacity of the protein free plasma in two independent studies of 6 and 10 subjects, respectively. Methods are described for application of the assay techniques to other types of biological and food samples...
- High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format
Dejian Huang
Brunswick Laboratories, 6 Thatcher Lane, Wareham, Massachusetts 02571, USA
J Agric Food Chem 50:4437-44. 2002
..The mean of intra- and interday CVs was <or=15%, and the limit of detection and limit of quantitation were 5 and 6.25 microM, respectively...
- Antioxidant activity and phenolic content of Oregon caneberries
Leslie Wada
LW Consulting, Suite 238, 1499 Bayshore Highway, Burlingame, California 94010, USA
J Agric Food Chem 50:3495-500. 2002
..The results from this study indicate that these caneberries were high in antioxidant activity and were rich sources of anthocyanins and phenolics...
- Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe
Boxin Ou
Brunswick Laboratories, 6 Thatcher Lane, Wareham, Massachusetts 02571, USA
J Agric Food Chem 50:2772-7. 2002
..A wide range of phenolic antioxidants is analyzed, and the hydroxyl radical prevention capacity is mainly due to the metal-chelating capability of the compounds...
- Development and validation of oxygen radical absorbance capacity assay for lipophilic antioxidants using randomly methylated beta-cyclodextrin as the solubility enhancer
Dejian Huang
Brunswick Laboratories, 6 Thacher Lane, Wareham, Massachusetts 02571, USA
J Agric Food Chem 50:1815-21. 2002
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- Impact of alkalization on the antioxidant and flavanol content of commercial cocoa powders
Kenneth B Miller
Hershey Center for Health and Nutrition, The Hershey Company, 1025 Reese Avenue, Hershey, Pennsylvania 17033, USA
J Agric Food Chem 56:8527-33. 2008
..The observed linear and predictable impact of alkalization on flavanol content is discussed with respect to other reports in the literature as well as what implications it may have on diet and food manufacturing...
