Target identification of drug induced mitochondrial toxicity using immunocapture based OXPHOS activity assaysSashi Nadanaciva
MitoSciences Inc, 1850 Millrace Drive, Eugene, OR 97403, USA
Toxicol In Vitro 21:902-11. 2007
..A major advantage of the immunocapture approach is that it can be used throughout drug screening from early compound evaluation to clinical trials...
Mitochondrial impairment by PPAR agonists and statins identified via immunocaptured OXPHOS complex activities and respirationSashi Nadanaciva
MitoSciences, Inc, 1850 Millrace Drive, Eugene, OR 97403, USA
Toxicol Appl Pharmacol 223:277-87. 2007
..In several cases, the combination additively uncoupled or inhibited respiration, suggesting that some combinations are more likely to yield clinically relevant drug-induced mitochondrial side effects than others...
Lateral-flow immunoassay for detecting drug-induced inhibition of mitochondrial DNA replication and mtDNA-encoded protein synthesisSashi Nadanaciva
MitoSciences Inc, Eugene, OR 97403, USA
J Immunol Methods 343:1-12. 2009
..The assay has high reproducibility, requires small amounts of sample, is quantitative, and is able to identify drugs which ultimately lead to a decrease in mtDNA-encoded proteins...
The molecular mechanism of ATP synthesis by F1F0-ATP synthaseAlan E Senior
Department of Biochemistry and Biophysics, University of Rochester Medical Center, Box 712, 601 Elmwood Avenue, Rochester, NY 14642, USA
Biochim Biophys Acta 1553:188-211. 2002
..In this review we summarize current state of knowledge and present a hypothesis for the molecular mechanism of ATP synthesis...
In vitro assessment of mitochondrial dysfunction and cytotoxicity of nefazodone, trazodone, and buspironeJames A Dykens
Drug Safety Research and Development, Pfizer, Inc, Sandwich CT139NJ, UK
Toxicol Sci 103:335-45. 2008
..Our results suggest that the mitochondrial impairment imposed by nefazodone is profound and likely contributes to its hepatotoxicity, especially in patients cotreated with other drugs with mitochondrial liabilities...