Research Topics
Genomes and GenesSpecies | C F AriasSummaryCountry: Mexico Publications
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Publications
Characterization of viroplasm formation during the early stages of rotavirus infectionJosé J Carreño-Torres
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico
Virol J 7:350. 2010..The possibility that viroplasms formation is nucleated by the entering viral particles was investigated using fluorescently labeled purified rotavirus particles...
Comparative study of enteric viruses, coliphages and indicator bacteria for evaluating water quality in a tropical high-altitude systemAna C Espinosa
Instituto de Ecologia, Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Universitaria, Coyoacan, 04510 Mexico, DF, Mexico
Environ Health 8:49. 2009..Surface water from a tropical high-altitude system located in Mexico City that receives rainwater, treated and non-treated wastewater used for irrigation, and groundwater used for drinking, was studied...
Molecular biology of rotavirus cell entryCarlos F Arias
Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
Arch Med Res 33:356-61. 2002..We propose that the functional rotavirus receptor is a complex of several cell molecules most likely immersed in plasma membrane lipid microdomains...
Molecular anatomy of 2009 influenza virus A (H1N1)Carlos F Arias
Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
Arch Med Res 40:643-54. 2009....
Trypsin activation pathway of rotavirus infectivityC F Arias
Departamento de Genetica y Fisiologia Molecular, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
J Virol 70:5832-9. 1996..A pathway for the trypsin activation of rotavirus infectivity is proposed...
Characterization of a monoclonal antibody directed to the surface of MA104 cells that blocks the infectivity of rotavirusesS Lopez
Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia
Virology 273:160-8. 2000..By a solid-phase immunoisolation technique, MAb 2D9 was found to react with three proteins of ca. 47, 55, and 220 kDa, which might form a complex...
Early events of rotavirus infection: the search for the receptor(s)C F Arias
, , , Cuernavaca, Morelos 62250, Mexico
Novartis Found Symp 238:47-60; discussion 60-3. 2001..We propose that the functional rotavirus receptor is a complex of several cell molecules most likely immersed in glycosphingolipid-enriched plasma membrane microdomains...
Functional and structural analysis of the sialic acid-binding domain of rotavirusesP Isa
Departamento de Genetica y Fisiologia Molecular, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos, Mexico
J Virol 71:6749-56. 1997..The close proximity of these two loops is also supported by previous results from competition experiments with neutralizing MAbs directed at RRV VP8...
Rotavirus Nonstructural Protein NSP3 is not required for viral protein synthesisHilda Montero
, UNAM, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico
J Virol 80:9031-8. 2006..These results indicate that NSP3 is neither required for the translation of viral mRNAs nor essential for virus replication in cell culture...
Rotaviruses induce an early membrane permeabilization of MA104 cells and do not require a low intracellular Ca2+ concentration to initiate their replication cycleM A Cuadras
Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos
J Virol 71:9065-74. 1997....
RNA silencing of rotavirus gene expressionCarlos F Arias
, , , Avenida Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico
Virus Res 102:43-51. 2004..Although the use of RNAi as an antiviral therapeutic tool remains to be demonstrated, there is no doubt that this technology will influence drastically the way postgenomic virus research is conducted...
Analysis of the kinetics of transcription and replication of the rotavirus genome by RNA interferenceCamilo Ayala-Breton
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, UNAM, Cuernavaca, Morelos 62210, Mexico
J Virol 83:8819-31. 2009....
Early steps in rotavirus cell entryS Lopez
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, 62210 Cuernavaca, Mexico
Curr Top Microbiol Immunol 309:39-66. 2006..This review focuses on recent data describing the interactions between the virus and its receptors, the role of lipid microdomains in rotavirus infection, and the possible mechanism of rotavirus cell entry...
Infectivity and genome persistence of rotavirus and astrovirus in groundwater and surface waterAna Cecilia Espinosa
Instituto de Ecologia, Universidad Nacional Autonoma de Mexico UNAM, Tercer Circuito Escolar, Ciudad Universitaria, Coyoacan 04510, Mexico DF
Water Res 42:2618-28. 2008..78 and 1.3 logs, respectively. The longer persistence of viruses in this study could result from a combination of factors, including aggregation of the virus...
Endoplasmic reticulum chaperones are involved in the morphogenesis of rotavirus infectious particlesLiliana Maruri-Avidal
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62210, Mexico
J Virol 82:5368-80. 2008..The complexity of the steps of rotavirus assembly that occur in the ER provide a useful model for studying the organization and operation of the complex network of chaperones involved in maintaining the quality control of this organelle...
Association of the astrovirus structural protein VP90 with membranes plays a role in virus morphogenesisErnesto Méndez
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apartado Postal 510 3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico
J Virol 81:10649-58. 2007....
Rotavirus infection induces the phosphorylation of eIF2alpha but prevents the formation of stress granulesHilda Montero
Instituto de Biotecnologia, UNAM, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos 62210, Mexico
J Virol 82:1496-504. 2008....
Role of sialic acids in rotavirus infectionPavel Isa
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Cuernavaca, Morelos 62210, Mexico
Glycoconj J 23:27-37. 2006....
Caspases mediate processing of the capsid precursor and cell release of human astrovirusesErnesto Méndez
Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Apartado Postal 510 3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico
J Virol 78:8601-8. 2004..This is the first report that describes the induction and utilization of caspase activity by a virus to promote processing of the capsid precursor and dissemination of the viral particles...
Proteolytic processing of a serotype 8 human astrovirus ORF2 polyproteinErnesto Méndez
Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico
J Virol 76:7996-8002. 2002..Based on these data, the processing pathway for the 90-kDa primary product of astrovirus Yuc8 ORF2 is presented...
Protein products of the open reading frames encoding nonstructural proteins of human astrovirus serotype 8Ernesto Méndez
Departamento de Genetica y Fisiologia Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico
J Virol 77:11378-84. 2003..Based on the differential reactivities of the astrovirus proteins with the various antisera used, the individual polypeptides detected were mapped to the virus ORF1a and ORF1b regions...
Rotavirus RRV associates with lipid membrane microdomains during cell entryPavel Isa
, , , Cuernavaca, Morelos CP 62210, Mexico
Virology 322:370-81. 2004....
Dissecting the role of integrin subunits alpha 2 and beta 3 in rotavirus cell entry by RNA silencingPavel Isa
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001, Col Chamilpa, CP 62210 Cuernavaca, Morelos, Mexico
Virus Res 145:251-9. 2009..Altogether these data suggest that integrins alpha2 and alpha v beta 3 do not play a major role in the rotavirus entry process...
Rotaviruses require basolateral molecules for efficient infection of polarized MDCKII cellsMauricio Realpe
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, Av Universidad 2001, Col Chamilpa, Cuernavaca, Morelos CP 62210, Mexico
Virus Res 147:231-41. 2010....
Influence of calcium on the early steps of rotavirus infectionVictoria Pando
, , Cuernavaca, 62250, Mexico
Virology 295:190-200. 2002....
Heat shock cognate protein 70 is involved in rotavirus cell entryCarlos A Guerrero
, , , Cuernavaca, Morelos 62250, Mexico, USA
J Virol 76:4096-102. 2002..We propose that the functional rotavirus receptor is a complex of several cell surface molecules that include, among others, hsc70...
Characterization of a persistent respiratory syncytial virus showing a low-fusogenic activity associated to an impaired F proteinR E Sarmiento
Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Mexico D F 04510, Mexico
Virus Res 139:39-47. 2009....
Different rotavirus strains enter MA104 cells through different endocytic pathways: the role of clathrin-mediated endocytosisMichelle Gutierrez
Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnologia, UNAM, Avenida Universidad 2001, Col Chamilpa, Cuernavaca, Morelos 62210, Mexico
J Virol 84:9161-9. 2010..The inhibition of other endocytic mechanisms, such as macropinocytosis or caveola-mediated uptake, had no effect on the internalization of the rotavirus strains tested here...
Rotavirus diarrhea severity is related to the VP4 type in Mexican childrenFelipe Mota-Hernández
Departamento de Medicina Comunitaria e Hidratación Oral, Hospital Infantil de Mexico Federico Gomez, Mexico City, Mexico
J Clin Microbiol 41:3158-62. 2003..The relationship between serotype and clinical outcomes seems to be complex and to vary among different geographic areas...
The rotavirus surface protein VP8 modulates the gate and fence function of tight junctions in epithelial cellsPorfirio Nava
Department of Physiology, Biophysics and Neuroscience, Center for Research and Advanced Studies (CINVESTAV, Mexico DF 07000, Mexico
J Cell Sci 117:5509-19. 2004..VP8 given orally to diabetic rats allows the enteral administration of insulin, thus indicating that it can be employed to modulate epithelial permeability...
