Directed differentiation of red blood cells from human embryonic stem cellsShi Jiang Lu
Advanced Cell Technology, Worcester, MA, USA
Methods Mol Biol 636:105-21. 2010
..The homogeneous erythroid cells can be used for further mechanism studies...
- GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes
Shi Jiang Lu
Advanced Cell Technology, Worcester, MA 01605, USA
Genome Biol 8:R240. 2007
..Blast cells are multipotent and can differentiate into multiple lineages of hematopoeitic cells (erythroid, granulocyte and macrophage), endothelial and smooth muscle cells...
- Human embryonic stem cell lines generated without embryo destruction
Young Chung
Cell Stem Cell 2:113-7. 2008
- Robust generation of hemangioblastic progenitors from human embryonic stem cells
Shi Jiang Lu
Advanced Cell Technology, 381 Plantation Street, Worcester, MA 01605, USA
Regen Med 3:693-704. 2008
..However, successful preclinical and clinical progress requires efficient and controlled differentiation towards the specific differentiated cell fate...
- Recombinant HoxB4 fusion proteins enhance hematopoietic differentiation of human embryonic stem cells
Shi Jiang Lu
Advanced Cell Technology, Worcester, MA 01605, USA
Stem Cells Dev 16:547-59. 2007
..In summary, our study demonstrates that the tPTD-HoxB4 protein can be used with other recombinant proteins to efficiently generate transplantable HSCs from human ES cells...
- Hemangioblastic derivatives from human induced pluripotent stem cells exhibit limited expansion and early senescence
Qiang Feng
Stem Cell and Regenerative Medicine International, Worcester, Massachusetts, USA
Stem Cells 28:704-12. 2010
..Before clinical application, it will be necessary to determine the cause and extent of such abnormalities and whether they also occur in hiPSCs generated using different reprogramming methods...
- Biologic properties and enucleation of red blood cells from human embryonic stem cells
Shi Jiang Lu
Advanced Cell Technology, Worcester, MA, USA
Blood 112:4475-84. 2008
..The results show that it is feasible to differentiate and mature hESCs into functional oxygen-carrying erythrocytes on a large scale...
- Hemangioblasts from human embryonic stem cells generate multilayered blood vessels with functional smooth muscle cells
Shi Jiang Lu
Advanced Cell Technology, Worcester, Massachusetts, MA 01605, USA
Regen Med 4:37-47. 2009
....
- Derivation of human embryonic stem cells from single blastomeres
Irina Klimanskaya
Advanced Cell Technology, Worcester, Massachusetts 01605, USA
Nat Protoc 2:1963-72. 2007
..The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3-4 months...
- Generation of functional hemangioblasts from human embryonic stem cells
Shi Jiang Lu
Advanced Cell Technology, Worcester, Massachusetts 01605, USA
Nat Methods 4:501-9. 2007
..Our data suggest that hES-derived blast cells (hES-BCs) could be important in vascular repair...
- Hematopoietic cells from primate embryonic stem cells
Fei Li
Advanced Cell Technology, Biotech Five, Worcester, Massachusetts, USA
Methods Enzymol 418:243-51. 2006
..We have developed experimental conditions that promote efficient differentiation of these cells to produce progeny cells with considerable similarity to hematopoietic precursors harvested from bone marrow of adult animals...
- Human embryonic stem cell lines derived from single blastomeres
Irina Klimanskaya
Advanced Cell Technology, 381 Plantation Street, Worcester, Massachusetts 01605, USA
Nature 444:481-5. 2006
..The ability to create new stem cell lines and therapies without destroying embryos would address the ethical concerns of many, and allow the generation of matched tissue for children and siblings born from transferred PGD embryos...
- Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
Young Chung
Advanced Cell Technology, Worcester, Massachusetts 01605, USA
Nature 439:216-9. 2006
..The ability to generate human ES cells without the destruction of ex utero embryos would reduce or eliminate the ethical concerns of many...
- CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression pattern
Shi Jiang Lu
Department of Pediatrics M C 856, College of Medicine, University of Illinois at Chicago, 840 South Wood St, Chicago, IL 60612, USA
Blood 103:4134-41. 2004
..These observations demonstrate that CD34(+)CD38(-) cells derived from ESCs correspond consistently to an early developmental stage at which the yolk sac and fetal liver are the primary sites of hematopoiesis...
- Identification of genes preferentially expressed in mammary epithelial cells of Copenhagen rat using subtractive hybridization and microarrays
Chengshi Quan
Department of Pediatrics, 2Cancer Center, College of Medicine, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA
Carcinogenesis 24:1593-9. 2003
..These results suggest that IL-2Ralpha and claudin-6 may function as tumor suppressors, particularly for breast cancer. However, this possibility needs further investigation...
- Hematopoietic progenitor cells derived from embryonic stem cells: analysis of gene expression
Shi Jiang Lu
Department of Pediatrics, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612, USA
Stem Cells 20:428-37. 2002
..These latter observations may reflect the presence of heterogeneous cell populations within the cell fractions that were compared, or they may represent variability among ES-cell-derived hematopoietic stem cells...
- Comparative gene expression in hematopoietic progenitor cells derived from embryonic stem cells
Shi-Jiang Lu
Department of Pediatrics, College of Medicine, University of Illinois at Chicago, 60612, USA
Exp Hematol 30:58-66. 2002
..These observations could reflect the presence of different subpopulations in the cell fractions that were compared, or they may represent altered biologic properties of ES cell-derived hematopoietic stem cells...
