Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcriptsJoshua Z Levin
Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA
Genome Biol 10:R115. 2009
..Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes...
Comprehensive comparative analysis of strand-specific RNA sequencing methodsJoshua Z Levin
Broad Institute of Massachusetts Institute of Technology and Harvard University, Cambridge, Massachusetts, USA
Nat Methods 7:709-15. 2010
..Our analysis provides a comprehensive benchmark, and our computational pipeline is applicable for assessment of future protocols in other organisms...
Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast speciesMoran Yassour
Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA
Genome Biol 11:R87. 2010
..However, the functional role of antisense transcripts has been demonstrated only in a few cases and it has been suggested that most antisense transcripts may result from promiscuous bi-directional transcription in a dense genome...
Integrative analysis of the melanoma transcriptomeMichael F Berger
The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA
Genome Res 20:413-27. 2010
..Taken together, these results may indicate new avenues for target discovery in melanoma, while also providing a template for large-scale transcriptome studies across many tumor types...
Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cellsMichal Rabani
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
Nat Biotechnol 29:436-42. 2011
..Certain transcripts, including those encoding cytokines and transcription factors, mature faster. Our study provides a quantitative approach to study the integrative process of RNA regulation...
Full-length transcriptome assembly from RNA-Seq data without a reference genomeManfred G Grabherr
Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts, USA
Nat Biotechnol 29:644-52. 2011
..Our approach provides a unified solution for transcriptome reconstruction in any sample, especially in the absence of a reference genome...
Ab initio reconstruction of cell type-specific transcriptomes in mouse reveals the conserved multi-exonic structure of lincRNAsMitchell Guttman
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA
Nat Biotechnol 28:503-10. 2010
..Our results open the way to direct experimental manipulation of thousands of noncoding RNAs and demonstrate the power of ab initio reconstruction to render a comprehensive picture of mammalian transcriptomes...
Systematic identification of long noncoding RNAs expressed during zebrafish embryogenesisAndrea Pauli
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA
Genome Res 22:577-91. 2012
..Our study provides the first systematic identification of lncRNAs in a vertebrate embryo and forms the foundation for future genetic, genomic, and evolutionary studies...