Research Topics
Species | Sun Shin ChaSummaryAffiliation: Korea Ocean Research and Development Institute Country: Korea Publications
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Detail Information
Publications
Crystal structure of filamentous aggregates of human DJ-1 formed in an inorganic phosphate-dependent mannerSun Shin Cha
Marine and Extreme Genome Research Center, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
J Biol Chem 283:34069-75. 2008..According to the 2.4-A crystal structure, DJ-1 dimers are linearly stacked through P(i)-mediated interactions to form protofilaments, which are then bundled into a filamentous assembly...
Crystal structure of Lon protease: molecular architecture of gated entry to a sequestered degradation chamberSun Shin Cha
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan, Republic of Korea
EMBO J 29:3520-30. 2010..The bowl-shaped proteolytic chamber is contiguous with the chaperone chamber allowing internalized proteins direct access to the proteolytic sites without further gating restrictions...
Crystallization and preliminary X-ray crystallographic analysis of Lon from Thermococcus onnurineus NA1Young Jun An
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:54-6. 2010..The crystals belonged to space group P6(3), with unit-cell parameters a = 121.45, b = 121.45, c = 195.24 A. Assuming the presence of two monomers in the asymmetric unit, the solvent content was estimated to be about 60.7%...
Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognitionYoung Jun An
Marine and Extreme Genome Research Center, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
Nucleic Acids Res 37:3442-51. 2009..Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family...
Crystallization and preliminary X-ray crystallographic analysis of a novel histidinol-phosphate phosphatase from Thermococcus onnurineus NA1Ha Il Jung
Marine and Extreme Genome Research Center, Korea Ocean Research and Development Institute, Ansan, Republic of Korea
Acta Crystallogr Sect F Struct Biol Cryst Commun 65:472-4. 2009..The TON-HolPase crystals belonged to space group P222(1), with unit-cell parameters a = 40.88, b = 46.89, c = 148.03 A. Assuming the presence of one molecule in the asymmetric unit, the solvent content was estimated to be about 48.3%...
A novel inorganic pyrophosphatase in Thermococcus onnurineus NA1Hyun Sook Lee
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan, Seoul, Korea
FEMS Microbiol Lett 300:68-74. 2009..The site-specific mutation of the Gly residue in motif II to Ala or Ser residue exhibited only a slight change in the enzymatic activity and the K(m) value...
The complete genome sequence of Thermococcus onnurineus NA1 reveals a mixed heterotrophic and carboxydotrophic metabolismHyun Sook Lee
Korea Ocean Research and Development Institute, Ansan, P O Box 29, Seoul 425 600, South Korea
J Bacteriol 190:7491-9. 2008..onnurineus NA1 potentially allowing it to cope with nutrient stress in the surrounding of hydrothermal vents, providing the first genomic evidence for the carboxydotrophy in Thermococcus...
Approaches for novel enzyme discovery from marine environmentsHyun Sook Lee
Korea Ocean Research and Development Institute, Ansan, PO Box 29, Seoul 425 600, South Korea
Curr Opin Biotechnol 21:353-7. 2010....
Novel metagenome-derived carboxylesterase that hydrolyzes ?-lactam antibioticsJeong Ho Jeon
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan, South Korea
Appl Environ Microbiol 77:7830-6. 2011....
Crystallization and preliminary X-ray crystallographic analyses of Nur, a nickel-responsive transcription regulator from Streptomyces coelicolorYoung Jun An
Marine Biotechnology and New Material Research Division, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:130-2. 2008..The Nur crystals belong to space group P3(1), with unit-cell parameters a = b = 78.17, c = 50.39 A. Assuming the presence of two molecules in the asymmetric unit, the solvent content is estimated to be about 54.7%...
Dissection of the dimerization modes in the DJ-1 superfamilyHoi Jong Jung
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan, 426 744, Korea
Mol Cells 33:163-71. 2012..The observed phyletic pattern suggests the dynamic nature of quaternary structures in the course of evolution. The amino acid residues at the interfaces tend to show lower mutation rates than those of non-interfacial surfaces...
Purification, crystallization and preliminary crystallographic analysis of a multiple cofactor-dependent DNA ligase from Sulfophobococcus zilligiiSupangat Supangat
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:1583-5. 2010..The crystals belonged to space group P1, with unit-cell parameters a=63.7, b=77.1, c=77.8?Å, ?=83.4, ?=82.4, ?=74.6°. Assuming the presence of two molecules in the unit cell, the solvent content was estimated to be about 53.4%...
Cultivation at 6-10°C is an effective strategy to overcome the insolubility of recombinant proteins in Escherichia coliJung Min Song
Marine Biology and Living Resources Research Department, Korea Ocean Research and Development Institute, Ansan 426 744, Republic of Korea
Protein Expr Purif 82:297-301. 2012..coli BL21(DE3). Consequently, E. coli cultivation at 6-10°C is an effective strategy for overcoming a major hurdle of the inclusion body formation...
