Yuko Ibuki

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..The DIGE system used in this experiment has advantages (including a high level of statistical confidence) for discovering new functional proteins related to the regulation of apoptosis...

..These findings suggested that benzene metabolites induced dysregulation of apoptosis due to caspase-3 inhibition, which contributes to carcinogenesis...

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..In radiotherapy, the control of NO levels both in whole-body and in the tumor is important to prevent irradiation-induced injury and for the cancer to regress...

..These results suggested the existence of two different survival pathways leading to suppression of apoptosis, one for UVA that is independent of the PI3-kinase-Akt pathway and the other for UVB and UVC that is dependent on this pathway...

..These findings indicated that UVA changes the flip-flop of phospholipids and that the cell membrane is a molecular and cellular target of UVA...

..These results suggested that UVB irradiation transduced a survival signal via PI3 kinase activation and phosphorylation of Akt, and induced some apoptosis inhibition factors upstream of the caspase cascade...

..This may contribute to the increase of cytolytic activity of macrophages after low-dose in vivo irradiation...

..These results indicated that preparation of DNA-TiO(2) at pH2 has advantages in that much DNA can be attached and large amounts of chemicals can be concentrated in the DNA, resulting in extensive decomposition under UV light...

..DSBs are the most serious form of DNA damage and are associated with genomic instability leading to myeloid leukemia. gamma-H2AX may be a useful tool for judging the genotoxicity of benzene metabolites sensitively...

..These results suggested that smaller TiO(2) particles were easy to incorporate into cells and generated cell cycle phase-independent ?-H2AX, which was dependent on the condition of the TiO(2) surface but not on the formation of ROS...

..UVB is a two-edged blade in environments, effectively degrading toxic chemicals but also producing genotoxic compounds as reactive intermediates...

..These findings suggested that H2O2 was essential to the inhibition of apoptosis, in which DNA damage had no role...

..These findings demonstrate that exposure of crude oil to sunlight makes the WSF phototoxic, generating DSBs accompanying the appearance of gamma-H2AX in human skin cells...

..These results suggested that DNA-TiO2 is beneficial for the removal and degradation of chemicals having affinity for DNA and dispersing in a large field...

..In addition, 3-OHBPA induced apoptosis similar to UVB- or UVC-irradiated ClBPA. These results suggested that the photoproducts of ClBPAs generated by UV irradiation, containing 3-OHBPA, contributed to the induction of apoptosis...

..These findings suggest that appropriate intracellular oxidation after UVB irradiation prevented apoptosis, a process which might be partially regulated by the production of reactive oxygen species mediated by Akt1...

..The appearance of gamma-H2AX means the induction of the most serious form of DNA damage, DSBs, suggesting the potential risk of carcinogenesis...

..These findings suggest that histone H2AX is a potential moleculartargetfor detecting the phototoxicity of PAHs more sensitively than the detection of cell viability and DSBs...

..The generation of ?-H2AX means the formation of DSBs, the worst type of DNA damage. The results indicated that attention should be paid to degradated short chain NPEOs and their genotoxicity...

..Our findings indicate that coexposure to BaP and UVA effectively induced DNA damage, especially DSBs, which results in phototoxicity and possibly photocarcinogenesis...

..Considering that the phosphorylation of histone H2AX is required for maintaining the genome's stability and the repair of DNA, attention to photoproducts from chlorinated compounds is important for the risk evaluation of chemicals...

..NaN(3) effectively inhibited the formation of gamma-H2AX induced by co-exposure, indicating the contribution of singlet oxygen. This is the first evidence that co-exposure to BaP and UVA induced DSBs, involving gamma-H2AX...

..DNA is a useful tool for separating TiO(2) particles which would prevent "small-size specific toxicity" by allowing quick and complete recovery and suppression of uptake by living organisms...

..The results suggested that the carcinogenicity of PAHs may be attributable not only to the genetic damage induced by their metabolites, but also to the antiapoptotic effects of oxidative products on exposure to sunlight...

..This study shows the significance of UV exposure to the degradation of alkylphenol polyethoxylates in the environment...

..H2AX was not generated following treatments with nonphototoxic chemicals and UVA. These results indicated that ?-H2AX is a powerful tool for detecting chemical photogenotoxicity...

..CSS in fact induced DSBs, which was also inhibited by NAC. DSBs are the worst type of DNA damage, related to genomic instability and carcinogenesis. Our results would increase the evidence of the strong genotoxicity of passive smoking...

..These findings suggested that UVB irradiation could decrease the estrogenic activity of chlorinated compounds, which was due to the selective release of chloride ion...

..Furthermore, we succeeded in visualizing that Ku80 rapidly assembled to the exposed region, in which DSBs might be generated, and clarified that the presence of both Ku70 and Ku80 was important for their accumulation...

..We concluded that (1) the increase in NO production was due to direct DNA damage by gamma-irradiation, and that (2) PARP activation through DNA damage induced NF-kappaB activation, leading to iNOS expression and NO production...

..Dysregulation of the apoptotic process is critical to carcinogenesis. The photosensitization of quinone compounds as it relates to the inhibition of apoptosis should be examined in the future...

..The uptake of other nanoparticles such as silver (Ag) and iron oxide (Fe3O4) also could be detected. This method could be used for the initial screening of the uptake potential of nanoparticles as an index of "nanotoxicity"...

..These results suggest that PS-induced apoptosis occurs without the collapse of mitochondrial transmembrane potential and without the release of cytochrome c, in a manner independent of caspase-1, -3, -8 and -9...

..These results indicated that the enhanced cell killing effect of PUVA by SB was attributable to an attenuated ability to repair DNA and, especially, dysfunctional NER...

..The present study demonstrates that zinc uptake is involved not only in the process of cell proliferation, but also in the process of apoptosis...

..Some PAHs were recently proved to be photomutagenic. In this review, we discuss the influence of PAHs in combination with sunlight focusing on the phototoxicity and cellular DNA damage produced...

..Recently, narrow-band ultraviolet B (UVB) phototherapy has been used to treat AD, but the mechanism involved is unknown. In this study, we investigated whether UVB irradiation influences AD in the NC/Nga mouse...

..The observations suggest that UVA reduces UVB-induced DNA damage, and may therefore have anti-photocarcinogenic properties that could be harnessed for better photoprotection in humans...