Research Topics
Genomes and Genes
| Hisao MasaiSummaryAffiliation: Tokyo Metropolitan Institute of Medical Science Country: Japan Publications
| Collaborators
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Detail Information
Publications
Cdc7 kinase complex: a key regulator in the initiation of DNA replicationHisao Masai
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Minato ku, Tokyo, Japan
J Cell Physiol 190:287-96. 2002..The interplay between Cdc7 and Cdk, another kinase essential for the S phase, is also discussed...- Phosphorylation of MCM4 by Cdc7 kinase facilitates its interaction with Cdc45 on the chromatinHisao Masai
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 281:39249-61. 2006..These results are consistent with the notion that the N-terminal phosphorylation of MCM2, MCM4, and MCM6 may play functionally redundant but essential roles in initiation of DNA replication... - Control of DNA replication: regulation and activation of eukaryotic replicative helicase, MCMHisao Masai
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
IUBMB Life 57:323-35. 2005.... - A second human Dbf4/ASK-related protein, Drf1/ASKL1, is required for efficient progression of S and M phasesNaoko Yoshizawa-Sugata
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 280:13062-70. 2005..Furthermore, mitotic progression is retarded in ASKL1 or Cdc7 siRNA-treated cells. Our results suggest that ASKL1 in a complex with Cdc7 may play a role in normal progression of both S and M phases... - Hsk1 kinase and Cdc45 regulate replication stress-induced checkpoint responses in fission yeastSeiji Matsumoto
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Cell Cycle 9:4627-37. 2010..The results suggest that Hsk1-mediated loading of Cdc45 onto replication origins may play important roles in replication stress-induced checkpoint... - Hsk1-Dfp1/Him1, the Cdc7-Dbf4 kinase in Schizosaccharomyces pombe, associates with Swi1, a component of the replication fork protection complexSeiji Matsumoto
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 280:42536-42. 2005..These data suggest that Hsk1-Dfp1/Him1 and Swi1-Swi3 complexes have interrelated roles in stabilization of arrested replication forks... - Roles of human AND-1 in chromosome transactions in S phaseNaoko Yoshizawa-Sugata
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
J Biol Chem 284:20718-28. 2009.... - Functional analyses of mouse ASK, an activation subunit for Cdc7 kinase, using conditional ASK knockout ES cellsNobuyuki Yamashita
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
Genes Cells 10:551-63. 2005..These results may suggest that motif-N of ASK may facilitate recruitment of substrates for Cdc7 kinase... - Hsk1 kinase is required for induction of meiotic dsDNA breaks without involving checkpoint kinases in fission yeastKeiko Ogino
Genome Dynamics Project and Department of Integrated Life Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
Proc Natl Acad Sci U S A 103:8131-6. 2006..These results indicate unique and essential roles of Hsk1 kinase in the initiation of meiotic recombination and meiosis... - Interactions between Swi1-Swi3, Mrc1 and S phase kinase, Hsk1 may regulate cellular responses to stalled replication forks in fission yeastMichie Shimmoto
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
Genes Cells 14:669-82. 2009..These results suggest that interactions of the Swi1-Swi3 complex and Hsk1 kinase with Mrc1 may play a role in cellular responses to stalled replication forks in fission yeast... - Molecular mechanism of activation of human Cdc7 kinase: bipartite interaction with Dbf4/activator of S phase kinase (ASK) activation subunit stimulates ATP binding and substrate recognitionRyo Kitamura
Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2 1 6 Kamikitazawa, Setagaya Ku, Tokyo 156 8506, Japan
J Biol Chem 286:23031-43. 2011..These results indicate bipartite interaction between Cdc7 and Dbf4/ASK subunits facilitates ATP binding and substrate recognition by the Cdc7 kinase... - Replication initiation from a novel origin identified in the Th2 cytokine cluster locus requires a distant conserved noncoding sequenceToshiro Hayashida
Cytokine Project, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, 113-8613 Tokyo, Japan
J Immunol 176:5446-54. 2006.... - Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpointNaoko Yoshizawa-Sugata
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 282:2729-40. 2007..Our results indicate that mammalian Tipin is a checkpoint mediator that cooperates with Tim and may regulate the nuclear relocation of Claspin in response to replication checkpoint... - Fission yeast Swi1-Swi3 complex facilitates DNA binding of Mrc1Taku Tanaka
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8506, Japan
J Biol Chem 285:39609-22. 2010..Our results reveal an aspect of molecular interactions that may play an important role in replication pausing and fork stabilization... - Escherichia coli PriA protein, two modes of DNA binding and activation of ATP hydrolysisTaku Tanaka
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 282:19917-27. 2007..We propose architecture of PriA bound to various arrested replication fork structures and discuss its implication in helicase activation and ATP hydrolysis... - Mrc1 marks early-firing origins and coordinates timing and efficiency of initiation in fission yeastMotoshi Hayano
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa 2 1 6, Setagaya Ku, Tokyo 156 8506, Japan
Mol Cell Biol 31:2380-91. 2011..We propose that prefiring binding of Mrc1 is an important marker of early-firing origins which are precociously activated by the absence of this protein... - Stabilization of a stalled replication fork by concerted actions of two helicasesTaku Tanaka
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan
J Biol Chem 281:3484-93. 2006..Our results present a novel mechanism by which two helicases function in a highly coordinated manner to generate a structure in which an arrested fork is stabilized for further repair and/or replication restart... - ATPase/helicase motif mutants of Escherichia coli PriA protein essential for recombination-dependent DNA replicationTaku Tanaka
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
Genes Cells 8:251-61. 2003..However, the roles of ATPase/DNA helicase activities in functions of PriA are not well understood... - Identification of stimulators and inhibitors of Cdc7 kinase in vitroNaoko Kakusho
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 283:19211-8. 2008.... - Cdt1 forms a complex with the minichromosome maintenance protein (MCM) and activates its helicase activityZhiying You
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
J Biol Chem 283:24469-77. 2008..Thus, a productive interaction between Cdt1 and MCM appears to be essential for efficient loading of MCM onto template DNA, as well as for the efficient unwinding reaction... - Cell cycle regulation of chromatin binding and nuclear localization of human Cdc7-ASK kinase complexNoriko Sato
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
Genes Cells 8:451-63. 2003..In mammals, it is not known at which time point during the cell cycle Cdc7 and Dbf4/ASK proteins are imported into nuclei and loaded on to chromatin... - Roles of Mcm7 and Mcm4 subunits in the DNA helicase activity of the mouse Mcm4/6/7 complexZhiying You
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 18 22 Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
J Biol Chem 277:42471-9. 2002..Komamura, Y., and Ishimi, Y. (1999) Mol. Cell. Biol. 19, 8003-8015), our data indicate distinct roles of Mcm4, Mcm6, and Mcm7 subunits in activation of the DNA helicase activity of the Mcm4/6/7 complex... - Thymine-rich single-stranded DNA activates Mcm4/6/7 helicase on Y-fork and bubble-like substratesZhiying You
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113-8613, Japan
EMBO J 22:6148-60. 2003..These findings lead us to propose that selective activation by stretches of thymine sequences of a fraction of Mcm helicases loaded onto chromatin may be the determinant for selection of initiation sites on mammalian genomes... - Rad3-Cds1 mediates coupling of initiation of meiotic recombination with DNA replication. Mei4-dependent transcription as a potential target of meiotic checkpointKeiko Ogino
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 281:1338-44. 2006.... - Cell cycle and developmental regulations of replication factors in mouse embryonic stem cellsHiroko Fujii-Yamamoto
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan
J Biol Chem 280:12976-87. 2005..Furthermore, they are rapidly down-regulated upon induction of differentiation of ES cells. The significance of these findings is discussed in relation to the unusual proliferative properties of ES cells in an undifferentiated state... - DNA binding and helicase actions of mouse MCM4/6/7 helicaseZhiying You
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science 18 22, Honkomagome 3 chome, Bunkyo ku, Tokyo 113 8613, Japan
Nucleic Acids Res 33:3033-47. 2005..Taken together, these findings reveal important features on activation and substrate preference of the eukaryotic replicative helicase... - Multiple pathways can bypass the essential role of fission yeast Hsk1 kinase in DNA replication initiationSeiji Matsumoto
Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Setagaya Ku, Tokyo 156 8613, Japan
J Cell Biol 195:387-401. 2011.... - Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and developmentJung Min Kim
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan
Mutat Res 532:29-40. 2003..Partial, rather than total, loss of Cdc7 kinase expression results in retarded growth at both cellular and whole body levels, with especially profound impairment of germ cell development... - Hypomorphic mutation in an essential cell-cycle kinase causes growth retardation and impaired spermatogenesisJung Min Kim
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo 113 8613, Japan
EMBO J 22:5260-72. 2003..Our results indicate the requirement of a critical level of a cell-cycle regulator for mouse development and provide genetic evidence that Cdc7 plays essential roles in meiotic processes in mammals... - Eukaryotic chromosome DNA replication: where, when, and how?Hisao Masai
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156 8506, Japan
Annu Rev Biochem 79:89-130. 2010..We review here various regulatory mechanisms that control the replication program in eukaryotes and discuss future directions in this dynamic field... - Efficient expression and purification of human replication fork-stabilizing factor, Claspin, from mammalian cells: DNA-binding activity and novel protein interactionsSyuzi Uno
Genome Dynamics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo 156 8506, Japan
Genes Cells 16:842-56. 2011..We will also discuss the advantage of this system for purification and characterization of those proteins that are large and have been difficult to deal with... - Stalled replication forks: making ends meet for recognition and stabilizationHisao Masai
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Bioessays 32:687-97. 2010..Elucidation of the structural basis for recognition of arrested forks by PriA should provide useful insight into how stalled forks are recognized in eukaryotes... - Cdc7 as a potential new target for cancer therapySayuri Ito
Genome Dynamics Project, Tokyo Metropolitan Institute of Medical Science, Bunkyo ku, Tokyo, Japan
Drug News Perspect 21:481-8. 2008..Thus, Cdc7 kinase may be a promising novel target for cancer therapy. Indeed, the first classes of Cdc7 inhibitors have been reported and have been shown to be effective in delaying tumor growth in animal models... - Genetic dissection of mammalian Cdc7 kinase: cell cycle and developmental rolesJung Min Kim
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan
Cell Cycle 3:300-4. 2004..These results from mammals will be discussed in conjunction with the pleiotropic effects of Cdc7 mutation observed in yeasts... - DNA binding of PriA protein requires cooperation of the N-terminal D-loop/arrested-fork binding and C-terminal helicase domainsTaku Tanaka
Department of Molecular and Developmental Biology, Institute of Medical Science, University of Tokyo, Tokyo 108 8639, Japan
J Biol Chem 277:38062-71. 2002.... - A 63-base pair DNA segment containing an Sp1 site but not a canonical E2F site can confer growth-dependent and E2F-mediated transcriptional stimulation of the human ASK gene encoding the regulatory subunit for human Cdc7-related kinaseMasayuki Yamada
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Tokyo 113 8613, Japan
J Biol Chem 277:27668-81. 2002..Our results suggest that E2F regulates the ASK promoter through an atypical mode of recognition of the target site... - Inactivation of Cdc7 kinase in mouse ES cells results in S-phase arrest and p53-dependent cell deathJung Min Kim
Department of Molecular and Developmental Biology, The Institute of Medical Science, The University of Tokyo, Minato ku, Tokyo 108 8639, CREST, Tokyo 108 8639, Japan
EMBO J 21:2168-79. 2002.... - Functional interaction between tumor suppressor menin and activator of S-phase kinaseRobert W Schnepp
Abramson Family Cancer Research Institute, Department of Cancer Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104 6160, USA
Cancer Res 64:6791-6. 2004..Together, these findings demonstrate a functional link between menin and ASK in the regulation of cell proliferation... - Identification and characterization of a Xenopus homolog of Dbf4, a regulatory subunit of the Cdc7 protein kinase required for the initiation of DNA replicationAsako Furukohri
Research Institute for Microbial Diseases, and Graduate School of Science, Osaka University, Suita, Osaka 565 0871
J Biochem 134:447-57. 2003..These results suggest that the function of XDbf4-XCdc7 during the early embryonic cell cycle is regulated in a manner distinct from that during the somatic cell cycle... - Human origins of DNA replication selected from a library of nascent DNAVesna Todorovic
Molecular Medicine Laboratory, International Centre for Genetic Engineering and Biotechnology, Padriciano, 99, 34012 Trieste, Italy
Mol Cell 19:567-75. 2005..This observation reinforces the universal paradigm that initiation of DNA replication takes place at, or in close proximity to, the binding sites of the trans-acting initiator proteins... - Cdc7-dependent phosphorylation of Mer2 facilitates initiation of yeast meiotic recombinationHiroyuki Sasanuma
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba 3 8 1, Meguro ku, Tokyo 153 8902, Japan
Genes Dev 22:398-410. 2008..We thus propose that Cdc7, in concert with CDK, regulates Spo11 loading to DSB sites via Mer2 phosphorylation... - Structural basis of the 3'-end recognition of a leading strand in stalled replication forks by PriAKaori Sasaki
Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan
EMBO J 26:2584-93. 2007..This unique feature is prerequisite for the proper positioning of the helicase domain of PriA on the unreplicated double-stranded DNA... - Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrestMegumi Kurita
Department of Pharmacology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
Biochem Biophys Res Commun 324:554-61. 2004.... - Crystallization and preliminary crystallographic analysis of the N-terminal domain of PriA from Escherichia coliKaori Sasaki
Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University, Maidashi 3 1 1, Fukuoka 812 8582, Japan
Biochim Biophys Acta 1764:157-60. 2006..We crystallized an N-terminal fragment of PriA in the absence and the presence of oligonucleotides to elucidate the structural basis for the specific recognition of the 3' terminus of DNA... - Nuclear import of Epstein-Barr virus nuclear antigen 1 mediated by NPI-1 (Importin alpha5) is up- and down-regulated by phosphorylation of the nuclear localization signal for which Lys379 and Arg380 are essentialRyo Kitamura
AIDS Research Center, National Institute of Infectious Diseases, Toyama 1 23 1, Shinjuku, Tokyo 162 8640, Japan
J Virol 80:1979-91. 2006.... - A critical role of the 3' terminus of nascent DNA chains in recognition of stalled replication forksToshimi Mizukoshi
Department of Structural Biology, Biomolecular Engineering Research Institute, Suita, Osaka 565 0874, Japan
J Biol Chem 278:42234-9. 2003..The results suggest a mechanism by which stalled replication forks are recognized by a sensor protein for checkpoint responses... - Visualizing spatiotemporal dynamics of multicellular cell-cycle progressionAsako Sakaue-Sawano
Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, RIKEN, 2 1 Hirosawa, Wako City, Saitama 351 0198, Japan
Cell 132:487-98. 2008..These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events...