Research Topics
| Toshio TomitaSummaryAffiliation: Tohoku University Country: Japan Publications
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Detail Information
Publications
Pleurotolysin, a novel sphingomyelin-specific two-component cytolysin from the edible mushroom Pleurotus ostreatus, assembles into a transmembrane pore complexToshio Tomita
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai 981 8555, Japan
J Biol Chem 279:26975-82. 2004..Pleurotolysin A and B formed an SDS-stable, ring-shaped complex of the same dimensions on sphingomyelin-cholesterol liposomes as well...- Bactericidal activity of a fermented hot-water extract from Stevia rebaudiana Bertoni towards enterohemorrhagic Escherichia coli O157:H7 and other food-borne pathogenic bacteriaT Tomita
Faculty of Agriculture, Tohoku University, Sendai, Miyagi, Japan
Microbiol Immunol 41:1005-9. 1997..In contrast, the fermented extract did not significantly kill Bifidobacteria or Lactobacilli. The active principle(s) of the fermented Stevia extract were bactericidal under acidic conditions... - Assembly of flammutoxin, a cytolytic protein from the edible mushroom Flammulina velutipes, into a pore-forming ring-shaped oligomer on the target cellT Tomita
Laboratory of Culture Collection, Institute of Medical Science, University of Tokyo, 4 6 1 Shirokanedai, Minato ku, Tokyo, Japan 108
Biochem J 333:129-37. 1998..These data indicated that flammutoxin assembles into a ring-shaped oligomer possessing a hydrophilic pore of 4-5 nm on target cells... - Protein sequence analysis, cloning, and expression of flammutoxin, a pore-forming cytolysin from Flammulina velutipes. Maturation of dimeric precursor to monomeric active form by carboxyl-terminal truncationToshio Tomita
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba ku, Sendai 981 8555, Japan
J Biol Chem 279:54161-72. 2004..In contrast, rFTX271, existing as a dimer in solution, bound to the cells but failed to form pore complex. The dimeric rFTX271 was converted to hemolytically active monomers upon the cleavage between Lys(251) and Met(252) by trypsin... - Cloning, expression, and pore-forming properties of mature and precursor forms of pleurotolysin, a sphingomyelin-specific two-component cytolysin from the edible mushroom Pleurotus ostreatusNobuki Sakurai
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan
Biochim Biophys Acta 1679:65-73. 2004..In contrast, the precursor of pleurotolysin B was much less hemolytically active than mature pleurotolysin B in the presence of pleurotolysin A... - Stochastic assembly of two-component staphylococcal gamma-hemolysin into heteroheptameric transmembrane pores with alternate subunit arrangements in ratios of 3:4 and 4:3Noriko Sugawara-Tomita
Department of Molecular and Cell Biology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan
J Bacteriol 184:4747-56. 2002.... - Cloning, expression, and cell surface localization of Paenibacillus sp. strain W-61 xylanase 5, a multidomain xylanaseYasuko Ito
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Aoba-ku, Sendai 981-8555, Japan
Appl Environ Microbiol 69:6969-78. 2003..Possible cooperative action of multiple xylanases produced by strain W-61 is discussed on the basis of the modular structure of Xyn5... - Complete nucleotide sequence and molecular characterization of the temperate staphylococcal bacteriophage phiPVL carrying Panton-Valentine leukocidin genesJ Kaneko
Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai 981 8555, Japan
Gene 215:57-67. 1998..aureus bacteriophages. The phiPVL genome was found to integrate into an ORF encoding an unknown protein comprising 725 amino acid residues with two leucine zipper-like motifs... - Escherichia coli heat-labile enterotoxin B subunits supplemented with a trace amount of the holotoxin as an adjuvant for nasal influenza vaccineS Tamura
Department of Pathology, National Institute of Health, Tokyo, Japan
Vaccine 12:1083-9. 1994..These results suggest that LTB containing a trace amount of LT can be used as a potent adjuvant for nasal vaccination of humans against influenza... - Bone marrow CD34+ progenitor cells from rheumatoid arthritis patients support spontaneous transformation of peripheral blood B cells from healthy individualsS Hirohata
Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan
Rheumatol Int 19:153-9. 2000..These results suggest that the capacity of BM CD34+ cells to support survival of B cells might contribute to the pathogenesis of RA by sustaining abnormal B cell responses... - Effect of a pore-forming protein derived from Flammulina velutipes on the Caco-2 intestinal epithelial cell monolayerAsako Narai
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan
Biosci Biotechnol Biochem 68:2230-8. 2004..These results suggest that TEER reduction could sensitively detect small membrane pore formation by FTX in the intestinal epithelium which causes a morphological alteration and disruption of the paracellular barrier function...