Research Topics
Genomes and Genes | K ShinozakiSummaryAffiliation: RIKEN Brain Science Institute Country: Japan Publications
| Collaborators
|
Detail Information
Publications
Identification of plant promoter constituents by analysis of local distribution of short sequencesYoshiharu Y Yamamoto
Application and Development Group, RIKEN FRS, Hirosawa 2 1, Wako, Saitama 351 0198, Japan
BMC Genomics 8:67. 2007....
Molecular responses to dehydration and low temperature: differences and cross-talk between two stress signaling pathwaysK Shinozaki
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Tsukuba, 305 0074, Japan
Curr Opin Plant Biol 3:217-23. 2000..Recent studies are providing evidence of differences between dehydration-signaling and cold-stress-signaling cascades, and of cross-talk between them...
Molecular responses to drought and cold stressK Shinozaki
Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, 3 1 1 Koyadai, Tsukuba, Ibaraki 305, Japan
Curr Opin Biotechnol 7:161-7. 1996..In addition, a number of genes for protein kinases and transcription factors thought to be involved in the stress signal transduction cascades have been shown to be induced by environmental stresses...
Isolation of ATMEKK1 (a MAP kinase kinase kinase)-interacting proteins and analysis of a MAP kinase cascade in ArabidopsisK Ichimura
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Japan
Biochem Biophys Res Commun 253:532-43. 1998..By contrast, other combinations of MAPKs and MAPKKs did not suppress these yeast mutations. These results suggest that ATMEKK1, ATMKK2/MEK1, and ATMPK4 may constitute a MAP kinase cascade...
A transmembrane hybrid-type histidine kinase in Arabidopsis functions as an osmosensorT Urao
Biological Resources Division, Japan International Research Center for Agricultural Science JIRCAS, Ministry of Agriculture, Forestry and Fisheries, 1 2 Oowashi, Tsukuba, Ibaraki 305, Japan
Plant Cell 11:1743-54. 1999..These results imply that ATHK1 functions as an osmosensor and transmits the stress signal to a downstream MAPK cascade...
Two genes that encode Ca(2+)-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thalianaT Urao
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Mol Gen Genet 244:331-40. 1994..The ATCDPK2 protein expressed in Escherichia coli was found to phosphorylate casein and myelin basic protein preferentially, relative to a histone substrate, and required Ca2+ for activation...
Characterization of two cDNAs that encode MAP kinase homologues in Arabidopsis thaliana and analysis of the possible role of auxin in activating such kinase activities in cultured cellsT Mizoguchi
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Tsukuba, Ibaraki, Japan
Plant J 5:111-22. 1994..These results suggest that auxin may function as an activator of plant MAP kinase homologues, as do various mitogens in animal systems...
A cdc5+ homolog of a higher plant, Arabidopsis thalianaT Hirayama
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Proc Natl Acad Sci U S A 93:13371-6. 1996..In addition, we cloned a PCR fragment corresponding to the DNA binding domain of human Cdc5-like protein. These results strongly suggest that Cdc5-like protein exists in all eukaryotes and may function in cell cycle regulation...
Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plantsK Yamaguchi Shinozaki
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan
Mol Gen Genet 236:331-40. 1993..Histochemical analysis of GUS activity revealed that the rd29A promoter functions in almost all the organs and tissues of vegetative plants during water deficiency...
An essential role of a TatC homologue of a Delta pH- dependent protein transporter in thylakoid membrane formation during chloroplast development in Arabidopsis thalianaR Motohashi
Plant Functional Genomics Group, The Institute of Physical and Chemical Research Japan RIKEN Genomic Sciences Center, 3 1 1 Koyadai, Tsukuba 305 0074, Japan
Proc Natl Acad Sci U S A 98:10499-504. 2001..These results indicate an essential role of cpTatC in chloroplast development, especially in thylakoid membrane formation...
A gene encoding a mitogen-activated protein kinase kinase kinase is induced simultaneously with genes for a mitogen-activated protein kinase and an S6 ribosomal protein kinase by touch, cold, and water stress in Arabidopsis thalianaT Mizoguchi
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Proc Natl Acad Sci U S A 93:765-9. 1996....
A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thalianaT Hirayama
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Japan
Proc Natl Acad Sci U S A 92:3903-7. 1995....
Structure and expression of two genes that encode distinct drought-inducible cysteine proteinases in Arabidopsis thalianaM Koizumi
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Gene 129:175-82. 1993..The transcription start points were determined by primer extension. Two conserved sequences were found in the promoter regions of the two genes...
Stress-responsive expression of genes for two-component response regulator-like proteins in Arabidopsis thalianaT Urao
Biological Resources Division, Japan International Research Center for Agricultural Science, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, Japan
FEBS Lett 427:175-8. 1998..Levels of ATRR3 and ATRR4 were not affected by stress treatments. These results suggest that ATRRs play distinct physiological roles in Arabidopsis, and that some are involved in stress responses...
An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expressionZ K Shinwari
Biological Resources Division, Japan International Research Center for Agricultural Sciences JIRCAS, 1 2, Ohwashi, Ibaraki, Tsukuba, 305 8686, Japan
Biochem Biophys Res Commun 250:161-70. 1998..The beta-glucuronidase (GUS) reporter gene driven by the DREB1 promoters was induced at transcriptional level by low temperature in transgenic Arabidopsis plants...
An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequenceT Urao
Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Plant Cell 5:1529-39. 1993..Binding was sequence specific, as indicated by a gel mobility shift experiment. These results suggest that a MYB-related transcription factor is involved in the regulation of genes that are responsive to water stress in Arabidopsis...
The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thalianaK Yamaguchi-Shinozaki
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Mol Gen Genet 238:17-25. 1993..thaliana genome, while the gene for USP from V. faba is actually a family of genes with 10 to 20 members.(ABSTRACT TRUNCATED AT 250 WORDS)..
Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditionsY Uno
Biological Resources Division, Japan International Research Center for Agricultural Science JIRCAS, Ministry of Agriculture, Forestry, and Fisheries, Ohwashi 1 2, Tsukuba, Ibaraki 305 8686, Japan
Proc Natl Acad Sci U S A 97:11632-7. 2000..Using cultured Arabidopsis cells, we demonstrated that a specific ABA-activated protein kinase of 42-kDa phosphorylated conserved N-terminal regions in the AREB proteins...
Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6K Ichimura
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Plant J 24:655-65. 2000..Kinetics during activation differ between these two MAP kinases. These results suggest that ATMPK4 and ATMPK6 are involved in distinct signal transduction pathways responding to these environmental stresses...
Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thalianaK Ichimura
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
DNA Res 5:341-8. 1998..The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones...
Hyperosmotic stress induces a rapid and transient increase in inositol 1,4,5-trisphosphate independent of abscisic acid in Arabidopsis cell cultureS Takahashi
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074 Japan
Plant Cell Physiol 42:214-22. 2001..Taken together, these results suggest the involvement of PI-PLC and Ins(1,4,5)P3 in an ABA-independent hyperosmotic-stress signal transduction pathway in higher plants...
A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in ArabidopsisT Kiyosue
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Plant Cell 8:1323-35. 1996..These results suggest that ERD5 encodes a precursor of proline dehydrogenase (oxidase), which is regulated at the level of mRNA accumulation in both dehydrated and rehydrated plants...
Two genes that encode ribosomal-protein S6 kinase homologs are induced by cold or salinity stress in Arabidopsis thalianaT Mizoguchi
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan
FEBS Lett 358:199-204. 1995....
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in ArabidopsisS Iuchi
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Plant J 27:325-33. 2001..We improved drought tolerance by gene manipulation of AtNCED3 causing the accumulation of endogenous ABA...
Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarrayM Seki
Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3 1 1 Koyadai, Tsukuba 305 0074, Japan
Plant Cell 13:61-72. 2001....
Functional cloning of a cDNA encoding Mei2-like protein from Arabidopsis thaliana using a fission yeast pheromone receptor deficient mutantT Hirayama
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
FEBS Lett 413:16-20. 1997..Mei2 is involved in the regulation of meiosis in fission yeast. Northern blot analysis showed that the AML1 gene is expressed in each organ. The possible functions of AML1 are discussed...
Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in ArabidopsisQ Liu
Biological Resources Division, Japan International Research Center for Agricultural Sciences JIRCAS, Ministry of Agriculture, Forestry, and Fisheries, 2 1 Ohwashi, Tsukuba, Ibaraki 305 8686, Japan
Plant Cell 10:1391-406. 1998..These results indicate that two independent families of DREB proteins, DREB1 and DREB2, function as trans-acting factors in two separate signal transduction pathways under low-temperature and dehydration conditions, respectively...
A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stressK Yamaguchi-Shinozaki
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Plant Cell 6:251-64. 1994..Different cis-acting elements seem to function in the two-step induction of rd29A and in the slow induction of rd29B under conditions of dehydration, high salt, or low temperature...
Involvement of a novel Arabidopsis phospholipase D, AtPLDdelta, in dehydration-inducible accumulation of phosphatidic acid in stress signallingT Katagiri
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Plant J 26:595-605. 2001..The accumulation of PtdOH in response to dehydration was significantly suppressed in AtPLDdelta antisense transgenic plants. These results suggest that AtPLDdelta may be involved in PtdOH accumulation in the dehydration stress response...
Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thalianaT Katagiri
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Inaraki 305, Japan
Plant Mol Biol 30:647-53. 1996..The ATDGK1 mRNA was detected in roots, shoots, and leaves. Southern blot analysis suggests that the ATDGK1 gene is a single-copy gene. The existence of DGK as well as phospholipase C suggests the existence of PKC in plants...
Possible His to Asp phosphorelay signaling in an Arabidopsis two-component systemT Urao
Biological Resources Division, Japan International Research Center for Agricultural Science JIRCAS, Ministry of Agriculture, Forestry and Fisheries, Ohwashi, Tsukuba, Ibaraki, Japan
FEBS Lett 478:227-32. 2000..However, ATHP1 could not interact with any ATRRs. On the basis of these results, we discuss the possible phosphorelay networks in an Arabidopsis two-component system...
ATMPKs: a gene family of plant MAP kinases in Arabidopsis thalianaT Mizoguchi
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
FEBS Lett 336:440-4. 1993..Genes for MAP kinases in Arabidopsis constitute a family that contains more than seven members. Sequence analysis suggests that there are at least three subfamilies in the family of Arabidopsis genes for MAP kinases...
Cloning and characterization of a plant gene encoding a protein kinaseN Hayashida
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan
Gene 124:251-5. 1993..From the results of sequence comparisons, the ATPK5 protein appears to be a member of a subfamily of Ser/Thr-PKs specific to plants...
Characterization of an Arabidopsis cDNA for a soluble epoxide hydrolase gene that is inducible by auxin and water stressT Kiyosue
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Plant J 6:259-69. 1994..e. stems and leaves), but not in roots, flowers and seeds. The possible function of AtsEH is discussed. A similar sEH cDNA has recently been characterized in potato (Stapleton et al., 1994)...
Identification of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutantsT Mizoguchi
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
FEBS Lett 437:56-60. 1998..These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants...
ERD6, a cDNA clone for an early dehydration-induced gene of Arabidopsis, encodes a putative sugar transporterT Kiyosue
Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research RIKEN, 3 1 1 Koyadai, Tsukuba, Ibaraki 305, Japan
Biochim Biophys Acta 1370:187-91. 1998..Genomic Southern blots indicate that the ERD6 gene is a member of a multigene family in the Arabidopsis genome. The expression of the ERD6 gene was induced not only by dehydration but also by cold treatment...
Characterization of two cDNAs (ERD10 and ERD14) corresponding to genes that respond rapidly to dehydration stress in Arabidopsis thalianaT Kiyosue
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Plant Cell Physiol 35:225-31. 1994....
A chromo box gene from carrot (Daucus carota l.): its cDNA structure and expression during somatic and zygotic embryogenesisT Kiyosue
Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research RIKEN, 3 1 1 Koyadai, Tsukuba, Ibaraki 305, Japan
Biochim Biophys Acta 1398:42-6. 1998..The level of DcCB1 transcripts decreased during the formation of seeds. The existence of both homeo and chromo box genes in plants suggests that regulatory mechanisms of developmental genes in plants may resemble those in Drosophila...
Cloning of cDNAs for genes that are early-responsive to dehydration stress (ERDs) in Arabidopsis thaliana L.: identification of three ERDs as HSP cognate genesT Kiyosue
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan
Plant Mol Biol 25:791-8. 1994..We also discuss the possible functions of these three ERD gene products against dehydration stress...
Rapid construction of a transcription map for a cosmid contig of Arabidopsis thaliana genome using a novel cDNA selection methodM Seki
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Japan
Plant J 12:481-7. 1997....
Oxidative stress activates ATMPK6, an Arabidopsis homologue of MAP kinaseT Yuasa
Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3 1 1 Koyadai, Tsukuba, Ibaraki, 305 0074 Japan
Plant Cell Physiol 42:1012-6. 2001..These results indicate that ATMPK6 is one of the candidates for signal mediators in response to abiotic or biotic sources for ROS in Arabidopsis...
Characterization of two cDNAs (ERD11 and ERD13) for dehydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana LT Kiyosue
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
FEBS Lett 335:189-92. 1993..The expression of the genes for ERD11 and ERD13 was induced by dehydration, but was not affected by the application of four plant growth regulators, 2,4-dichlorophenoxyacetic acid 6-benzylaminopurine, abscisic acid, or gibberellic acid...
AtPLC2, a gene encoding phosphoinositide-specific phospholipase C, is constitutively expressed in vegetative and floral tissues in Arabidopsis thalianaT Hirayama
Laboratory of Plant Molecular Biology, The Institute of Physical and Chemical Research RIKEN, Tsukuba, Ibaraki, Japan
Plant Mol Biol 34:175-80. 1997..The AtPLC2 mRNA was detected in vegetative and floral tissues. We determined the positions of these two PI-PLCs genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones...
An Arabidopsis gene encoding a Ca2+-binding protein is induced by abscisic acid during dehydrationS Takahashi
Laboratory of Plant Molecular Biology, RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
Plant Cell Physiol 41:898-903. 2000..The recombinant RD20 protein was shown to bind Ca2+. The transcription of RD20 gene was induced not only by drought but also by ABA and high salinity...
Cloning and characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thalianaT Mizoguchi
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, RIKEN The Institute of Physical and Chemical Research, Ibaraki, Japan
Plant Mol Biol 21:279-89. 1993..The expressed ATCKA1 protein phosphorylated casein using either ATP or GTP. This activity was inhibited by heparin, indicating that the expressed protein has activity similar to those reported for animal and yeast CKII...
ERD15, a cDNA for a dehydration-induced gene from Arabidopsis thalianaT Kiyosue
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Ibaraki, Japan
Plant Physiol 106:1707. 1994
A novel Arabidopsis thaliana dynamin-like protein containing the pleckstrin homology domainK Mikami
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Tsukuba Life Science Center, Ibaraki, Japan
J Exp Bot 51:317-8. 2000..Taken together, it is predicted that the mode of activation of ADL3 is different from those of other plant homologues...
Correlation between the induction of a gene for delta 1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stressY Yoshiba
Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Plant J 7:751-60. 1995..The AtP5CR gene was not induced to a significant extent by dehydration or high-salt stress. These observations suggest that the AtP5CS gene plays a principal role in the biosynthesis of proline in A. thaliana under osmotic stress...
Disruption of an Arabidopsis cytoplasmic ribosomal protein S13-homologous gene by transposon-mediated mutagenesis causes aberrant growth and developmentT Ito
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, The Institute of Physical and Chemical Research RIKEN, 3 1 1 Koyadai, Tsukuba, Ibaraki 305 0074, Japan
Plant J 22:257-64. 2000....
Plant histidine kinases: an emerging picture of two-component signal transduction in hormone and environmental responsesT Urao
Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS, Ministry of Agriculture, Forestry and Fisheries, 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan
Sci STKE 2001:RE18. 2001..Plants widely use two-component systems in the detection of, and signal transduction by, the growth regulators ethylene and cytokinin, as well as in their responses to environmental stimuli...
Cloning and characterization of seven cDNAs for hyperosmolarity-responsive (HOR) genes of Saccharomyces cerevisiaeT Hirayama
Laboratory of Plant Molecular Biology, Tsukuba Life Science Center, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Mol Gen Genet 249:127-38. 1995..In addition, Hog1p, one of the MAP kinases in yeast, appeared to be involved in the regulation of expression of HOR genes, although its function seemed to be insufficient for the overall regulation of expression of these genes...
Nucleotide sequence of a gene from Arabidopsis thaliana encoding a myb homologueK Shinozaki
Plant Molecular Biology Laboratory, Institute of Physical and Chemical Research RIKEN, Ibaraki, Japan
Plant Mol Biol 19:493-9. 1992..The putative DNA-binding domain of the ATMYB1 protein has higher homology with that of the human c-MYB protein than with those of other plant MYB proteins...
