Research Topics
Genomes and GenesSpecies | Sachihiro MatsunagaSummaryAffiliation: Osaka University Country: Japan Publications
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Publications
An anther- and petal-specific gene SlMF1 is a multicopy gene with homologous sequences on sex chromosomesSachihiro Matsunaga
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Japan
Genes Genet Syst 80:395-401. 2005..Genomic Southern hybridization revealed that SlMF1 was a multicopy gene with a Y chromosome-linked homologous sequence. PCR analyses with flow-sorted chromosomes showed that SlMF1 was localized on both autosomes and the X chromosome...- Sex chromosome-linked genes in plantsSachihiro Matsunaga
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan
Genes Genet Syst 81:219-26. 2006..However, more detailed studies will be required to help explain the basis for the fact that heteromorphic sex chromosomes in angiosperms are large... - Sex-specific cell division during development of unisexual flowers in the dioecious plant Silene latifoliaSachihiro Matsunaga
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, 5 1 5, Kashiwanoha, Kashiwa, Chiba, 277 8562 Japan
Plant Cell Physiol 45:795-802. 2004..Our analyses showed that differential activation and reduction of cell division strongly correlated with sex-specific promotion and cessation in the sex differentiation of unisexual flowers... - Duplicative transfer of a MADS box gene to a plant Y chromosomeSachihiro Matsunaga
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan
Mol Biol Evol 20:1062-9. 2003..Our results suggest that the acquisition of autosomal genes is an important event in the evolution of plant Y chromosomes... - Characterization of plant Aurora kinases during mitosisAkira Kawabe
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, 565 0871, Suita, Osaka, Japan
Plant Mol Biol 58:1-13. 2005..Our results indicate that plant Aurora kinases have different characters from that of Aurora kinases of other eukaryotes... - Identification and characterization of plant Haspin kinase as a histone H3 threonine kinaseDaisuke Kurihara
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565 0871, Japan
BMC Plant Biol 11:73. 2011..Although extensive analyses of Haspin have been carried out in yeast and animals, the function of Haspin in organogenesis remains unclear... - A nucleolar protein RRS1 contributes to chromosome congressionArni E Gambe
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan
FEBS Lett 583:1951-6. 2009..RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression... - H1.X with different properties from other linker histones is required for mitotic progressionHideaki Takata
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565 0871, Japan
FEBS Lett 581:3783-8. 2007..X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes... - Characterization and dynamic analysis of Arabidopsis condensin subunits, AtCAP-H and AtCAP-H2Satoru Fujimoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1, Yamadaoka, Suita, Osaka, 565 0871, Japan
Planta 222:293-300. 2005..Differences in localization of AtCAP-H and AtCAP-H2 at interphase also suggest their functional differentiation... - PHB2 protects sister-chromatid cohesion in mitosisHideaki Takata
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565 0871, Japan
Curr Biol 17:1356-61. 2007..Thus, our findings suggest that, in addition to the shugoshin, PHB2 is also required to protect the centromeric cohesion from phosphorylation by Plk1 during early mitosis and that its function is essential for proper mitotic progression... - The Y chromosome-specific STS marker MS2 and its peripheral regions on the Y chromosome of the dioecious plant Silene latifoliaKotaro Ishii
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha 5 1 5, Kashiwa, Chiba 277 8562, Japan
Genome 51:251-60. 2008..latifolia. These results suggest an accumulation of retrotransposons and segmental duplications in peripheral regions of MS2 during the early stage of sex chromosome evolution... - The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindlesNan Ma
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565 0871, Japan
J Cell Sci 124:394-404. 2011..Our finding indicates that SAF-A is a novel spindle regulator that plays an essential role in kinetochore-MT attachment and mitotic spindle organization... - The Arabidopsis SDG4 contributes to the regulation of pollen tube growth by methylation of histone H3 lysines 4 and 36 in mature pollenJoyce A Cartagena
Department of Biotechnology, Graduate School of Engineering, Osaka University 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
Dev Biol 315:355-68. 2008..Our results indicate that SDG4 is capable of modulating the expression of genes that function in the growth of pollen tube by methylation of specific lysine residues of the histone H3 in the vegetative nuclei... - Isolation and characterization of two homeodomain leucine zipper genes from the dioecious plant Silene latifoliaAmr Ageez
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan
Genes Genet Syst 78:353-61. 2003..These results suggest that SlHDL1 and SlHDL2 regulate specific targets in restricted regions leading to floral organ differentiation in S. latifolia... - Organization of the KpnI family of chromosomal distal-end satellite DNAs in Silene latifoliaYusuke Kazama
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba 277 8562, Japan
J Plant Res 116:317-26. 2003..Multi-colored fluorescent in situ hybridization was performed with the KpnI family and RMY1 probes under high stringency conditions. The results suggest that chromosome 7 is unique and that it carries the KpnI family at only one end... - The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNAKayoko Hayashihara
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565 0871, Japan
J Biol Chem 285:6498-507. 2010..Thus, we discovered that HP1-BP74 directly binds to HP1, and its middle region associates with linker DNA at the entry/exit site of nucleosomal DNA in vitro... - Aurora kinase is required for chromosome segregation in tobacco BY-2 cellsDaisuke Kurihara
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita 565-0871, Osaka, Japan
Plant J 48:572-80. 2006..The data obtained here suggest that plant Aurora kinases and H3S10ph/H3S28ph may have a role in chromosome segregation and metaphase/anaphase transition... - Nucleophosmin is required for chromosome congression, proper mitotic spindle formation, and kinetochore-microtubule attachment in HeLa cellsMohammed Abdullahel Amin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
FEBS Lett 582:3839-44. 2008..Loss of NPM thus causes severe mitotic defects and delayed mitotic progression. These findings indicate that NPM is essential for mitotic progression and cell proliferation... - Histone H2A mobility is regulated by its tails and acetylation of core histone tailsTsunehito Higashi
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka 2 1, Suita, Osaka 567 0871, Japan
Biochem Biophys Res Commun 357:627-32. 2007..Our results support a model in which core histone tails directly regulate transcription by interacting with nucleosome DNA via electrostatic interactions... - Depletion of nucleophosmin leads to distortion of nucleolar and nuclear structures in HeLa cellsMohammed Abdullahel Amin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
Biochem J 415:345-51. 2008..These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells... - Generation of monoclonal antibodies against chromosomal antigens that have a high sequence similarity between human and mouseTsunehito Higashi
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
J Biotechnol 120:262-72. 2005..These results indicate that the current strategy is effective for obtaining monoclonal antibodies useful for immunoblotting and/or immunofluorescent staining of human proteins, using the antigens with high homology to mouse proteins... - CCLS96.1, a member of a multicopy gene family, may encode a non-coding RNA preferentially transcribed in reproductive organs of Silene latifoliaRyuji Sugiyama
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo, Tokyo 113 0033, Japan
DNA Res 10:213-20. 2003..Genomic Southern hybridization showed that the S. latifolia genome contains numerous CCLS96.1 homologs. These results suggest that the transcripts of CCLS96.1 play some role as multiple non-coding RNAs in S. latifolia... - Fibrillarin, a nucleolar protein, is required for normal nuclear morphology and cellular growth in HeLa cellsMohammed Abdullahel Amin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
Biochem Biophys Res Commun 360:320-6. 2007..Our data suggest that fibrillarin would play a critical role in the maintenance of nuclear shape and cellular growth... - Visualization of mitotic HeLa cells by advanced polarized light microscopyAkihiro Morimoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka, Japan
Micron 39:635-8. 2008..Here, we report the potential of advanced polarized light imaging systems for imaging mitotic HeLa cells and the new insights obtained during this microscopic study... - Crystal structure of Pyrococcus horikoshii PPC protein at 1.60 A resolutionLinyen Lin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan
Proteins 67:505-7. 2007 - Characterization of a splicing variant of plant Aurora kinaseDaisuke Kurihara
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka, Japan
Plant Cell Physiol 48:369-74. 2007..The localization pattern of AtAUR2S was the same as that of AtAUR2. The findings suggest that AtAUR2S affects cell division by competing with AtAUR2... - Condensin II alleviates DNA damage and is essential for tolerance of boron overload stress in ArabidopsisTakuya Sakamoto
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo ku, Tokyo 113 8657, Japan
Plant Cell 23:3533-46. 2011.... - Distribution of interstitial telomere-like repeats and their adjacent sequences in a dioecious plant, Silene latifoliaWakana Uchida
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha 5 1 5, Kashiwa, Chiba 277 8562, Japan
Chromosoma 111:313-20. 2002..latifolia. The distribution of IAS-d is important evidence for past telomere-mediated chromosome rearrangements during the evolution of the sex chromosomes of S. latifolia... - Live cell imaging reveals plant aurora kinase has dual roles during mitosisDaisuke Kurihara
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka, 565 0871 Japan
Plant Cell Physiol 49:1256-61. 2008..The findings indicate that the plant Aurora kinase has dual roles; correction of aberrant kinetochore-microtubule attachment and dissociation of cohesin during chromosome alignment and segregation... - Nucleolin functions in nucleolus formation and chromosome congressionNan Ma
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita 565 0871, Osaka, Japan
J Cell Sci 120:2091-105. 2007..Taken together, our results indicate that nucleolin is required for nucleolus formation, and is also involved in chromosome congression and spindle formation... - Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamicsArni E Gambe
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565 0871, Japan
Cytometry A 71:286-96. 2007..The purpose of this research was therefore to create a classifier to be incorporated into a system that can automatically identify the cell-cycle phases in a given image... - Identification of a novel plant MAR DNA binding protein localized on chromosomal surfacesSatoru Fujimoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka, 565-0871, Japan
Plant Mol Biol 56:225-39. 2004..In addition, AHL1 is located on the surface of chromosomes during mitosis... - A comparative proteome analysis of human metaphase chromosomes isolated from two different cell lines reveals a set of conserved chromosome-associated proteinsHideaki Takata
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565 0871, Japan
Genes Cells 12:269-84. 2007..Consequently, the present proteome analyses together with previous information on chromosome proteins provide the comprehensive list of proteins essential for the metaphase chromosome architecture... - An upper limit of the ratio of DNA volume to nuclear volume exists in plantsSatoru Fujimoto
Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamadaoka, Suita, Japan
Genes Genet Syst 80:345-50. 2005..The existence of an upper limit of DNA volume ratio would present a basal parameter for the future insight into the nuclear organisation in higher plants... - Crystallization and preliminary X-ray crystallographic analysis of a conserved domain in plants and prokaryotes from Pyrococcus horikoshii OT3Linyen Lin
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871 Osaka, Japan
Acta Crystallograph Sect F Struct Biol Cryst Commun 61:414-6. 2005..69, c = 159.2 A. Data were obtained at 100 K, with diffraction being observed to a resolution of 1.7 A. A complete data set from crystals of the SeMet-substituted protein was also obtained... - A plant Y chromosome-STS marker encoding a degenerate retrotransposonMari Obara
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan
Genes Genet Syst 77:393-8. 2002..The MS4b sequence was not detected in the most closely related dioecious species, S. dioica. This suggests that a retrotransposon with the MS4b sequence has degenerated recently on the Y chromosome... - Morphological development of anthers induced by the dimorphic smut fungus Microbotryum violaceum in female flowers of the dioecious plant Silene latifoliaWakana Uchida
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Bldg FSB 601, 5 1 5, Kashiwanoha, Kashiwa, 277 8562 Chiba, Japan
Planta 218:240-8. 2003..The development of the endothecial layers and epidermis in the infected anther were morphologically normal... - LTR retrotransposons in the dioecious plant Silene latifoliaSachihiro Matsunaga
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan
Genome 45:745-51. 2002..latifolia, S. dioica, and S. diclinis, fell into two clusters. The evolutionary dynamics of retrotransposons implies that, in the genus Silene, dioecious species evolved recently from gynodioecious species... - Interstitial telomere-like repeats in the Arabidopsis thaliana genomeWakana Uchida
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan
Genes Genet Syst 77:63-7. 2002..These results show that the A. thaliana genome has undergone chromosomal rearrangements, such as end-fusions and segmental duplications... - Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MSMasanori Noda
Graduate School of Engineering, Osaka University, Suita 565 0871, Japan
Biochem J 436:101-12. 2011..The present study illustrates a rigorous procedure for the analysis of protein assembly and protein-protein interactions in solution... - Ultrastructural analysis of the behavior of the dimorphic fungus Microbotryum violaceum in fungus-induced anthers of female Silene latifolia flowersWakana Uchida
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba
Protoplasma 226:207-16. 2005..The sporogenous cell is probably sensitive to infection by the fungus, resulting in disruption. In addition, the fungus accelerates cell death in the anther and utilizes constituents of the dead host cell to form the mature teliospore... - ASURA (PHB2) Is Required for Kinetochore Assembly and Subsequent Chromosome CongressionMei Hann Lee
Department of Biotechnology, Graduate School of Engineering, Osaka University, GSE Common East 7 F, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan
Acta Histochem Cytochem 44:247-58. 2011..We concluded that ASURA is an essential protein for complete kinetochore development, although ASURA is not being integrated to the kinetochore. These results highlight the uniqueness of ASURA as a kinetochore protein... - RAPD isolation of a Y chromosome specific ORF in a dioecious plant, Silene latifoliaShunsuke Nakao
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Japan
Genome 45:413-20. 2002..ORF285 may be a clue to the origin of dioecy... - Proteome analysis of human metaphase chromosomesSusumu Uchiyama
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan
J Biol Chem 280:16994-7004. 2005..This analysis represents the first compositional view of human metaphase chromosomes and provides a protein framework for future research on this topic... - Obtaining transgenic plants using the bio-active beads methodHaibo Liu
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan
J Plant Res 117:95-9. 2004..Using this method, we have successfully transformed tobacco plants, screening by kanamycin resistance. The transformed genes in the transformants were confirmed by PCR and Southern hybridization... - Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifoliaEduard Kejnovsky
Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, CZ 612 65, Brno, Czech Republic
Genetica 128:167-75. 2006..Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues... - DNA methylation analysis of a male reproductive organ specific gene (MROS1) during pollen developmentBohuslav Janousek
Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno
Genome 45:930-8. 2002..The results obtained in the MROS1 gene are discussed in consequence with the immunohistochemical data showing a hypermethylation of DNA in the vegetative nucleus... - In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitationWataru Watanabe
National Institute of Advanced Industrial Science and Technology, Photonics Research Institute, 1 8 31, Midorigaoka, Ikeda, Osaka 563 8577, Japan
J Biomed Opt 13:031213. 2008..We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein... - Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2Yutaka Miyazawa
Plant Functions Laboratory, RIKEN, Hirosawa, Wako Shi, Saitama, 351 0198, Japan
J Exp Bot 53:2451-2. 2002.... - Males evolved from the dominant isogametic mating typeHisayoshi Nozaki
Curr Biol 16:R1018-20. 2006