Research Topics
Genomes and Genes | Yusuke KamachiSummaryAffiliation: Osaka University Country: Japan Publications
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Publications
Functional analysis of chicken Sox2 enhancers highlights an array of diverse regulatory elements that are conserved in mammalsMasanori Uchikawa
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Dev Cell 4:509-19. 2003..These functionally identified Sox2 enhancers exactly correspond to the extragenic sequence blocks conspicuously conserved between chicken and mammals, which are not discernible by sequence comparison among mammals...- Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assayYusuke Kamachi
Graduate School of Frontier Biosciences, Developmental Biology Group, Osaka University, Suita, Japan
Genesis 46:1-7. 2008..Our quantitative assessment method also reveals, however, that an effective and specific knockdown can be achieved when employing a strategy that takes advantage of the synergistic effect of double MOs used at low levels... - PAX6 and SOX2-dependent regulation of the Sox2 enhancer N-3 involved in embryonic visual system developmentMasashi Inoue
Graduate School of Frontier Biosciences, Osaka University, Yamadaoka 1 3, Suita, Osaka 565 0871, Japan
Genes Cells 12:1049-61. 2007..The observations suggest that gene activation mechanisms that depend on the cooperative interaction of SOX2 and PAX6 but with different thresholds of the factor levels are crucial for the regulation of visual system development... - Convergence of Wnt and FGF signals in the genesis of posterior neural plate through activation of the Sox2 enhancer N-1Tatsuya Takemoto
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Development 133:297-306. 2006..The enhancer N-1c is not affected by BMP signals. Thus, Wnt and FGF signals converge to activate Sox2 expression through the enhancer N-1c, revealing the direct involvement of the Wnt signal in the initiation of neural plate development... - Multiple N-cadherin enhancers identified by systematic functional screening indicate its Group B1 SOX-dependent regulation in neural and placodal developmentMiho Matsumata
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Dev Biol 286:601-17. 2005..Enhancers 1, 2 and 4 correspond to sequence blocks conserved between the chicken and mammalian genomes, but enhancers 3 and 5 are unique to the chicken... - Adaptation of GAL4 activators for GAL4 enhancer trapping in zebrafishEri Ogura
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
Dev Dyn 238:641-55. 2009..By the combination of these genetic elements, we have successfully developed enhancer trap lines that activate UAS-dependent reporter genes in a tissue-specific fashion that reflects trapped enhancer activities... - Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiationHisato Kondoh
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Int J Dev Biol 48:819-27. 2004..Thus, the regulation of SOX2 (and SOX1/3) and its partner factors, exemplified by Pax6, determines the spatio-temporal order of the occurrence of cell differentiation... - Evolution of non-coding regulatory sequences involved in the developmental process: reflection of differential employment of paralogous genes as highlighted by Sox2 and group B1 Sox genesYusuke Kamachi
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
Proc Jpn Acad Ser B Phys Biol Sci 85:55-68. 2009.... - Interplay of SOX and POU factors in regulation of the Nestin gene in neural primordial cellsShinya Tanaka
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Mol Cell Biol 24:8834-46. 2004..Evidence also suggests that such interactions are involved in the regulation of neural primordial cells... - B1 SOX coordinate cell specification with patterning and morphogenesis in the early zebrafish embryoYuichi Okuda
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
PLoS Genet 6:e1000936. 2010.... - The Pou5f1/Pou3f-dependent but SoxB-independent regulation of conserved enhancer N2 initiates Sox2 expression during epiblast to neural plate stages in vertebratesMakiko Iwafuchi-Doi
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Dev Biol 352:354-66. 2011..In contrast, the enhancer N2-mediated, POU factor-dependent activation of Sox2, without involvement of Sox2, is a phylogenetically conserved core mechanism that functions in gene regulatory networks at early embryonic stages... - Distinct roles of SOX2, Pax6 and Maf transcription factors in the regulation of lens-specific delta1-crystallin enhancerMayumi Muta
Laboratory of Developmental Biology, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan
Genes Cells 7:791-805. 2002..To clarify the individual roles of these transcription factors, we analysed their participation in regulation of the delta1-crystallin enhancer... - Triggering neural differentiation of ES cells by subtype switching of importin-alphaNoriko Yasuhara
Department of Cell Biology and Neuroscience, Graduate School of Medicine, Osaka University, 2 2 Yamada oka, Suita, Osaka 565 0871, Japan
Nat Cell Biol 9:72-9. 2007..This is the first study to propose that transport factors should be considered as major players in cell-fate determination... - Comparative genomic and expression analysis of group B1 sox genes in zebrafish indicates their diversification during vertebrate evolutionYuich Okuda
Graduate School of Frontier Biosciences, Osaka University, Suita, Japan
Dev Dyn 235:811-25. 2006..It seems likely, therefore, that each B1/G Sox gene has gained a distinct expression profile and function during vertebrate evolution... - Efficient identification of regulatory sequences in the chicken genome by a powerful combination of embryo electroporation and genome comparisonMasanori Uchikawa
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan
Mech Dev 121:1145-58. 2004.... - SOX-partner code for cell specification: Regulatory target selection and underlying molecular mechanismsHisato Kondoh
Graduate School of Frontier Biosciences, Osaka University, 1 3 Yamadaoka, Suita, Osaka 565 0871, Japan
Int J Biochem Cell Biol 42:391-9. 2010..In vivo evidence for and molecular mechanisms of the cell specification code attributed to the SOX-partner factor complexes are reviewed... - Hypogonadotropic hypogonadism in an adult female with a heterozygous hypomorphic mutation of SOX2Naoko Sato
Department of Endocrinology and Metabolism, National Research Institute for Child Health and Development, 2 10 1 Ohkura, Setagaya, Tokyo 157 8535, Japan
Eur J Endocrinol 156:167-71. 2007..Here, we report a further case with a heterozygous hypomorphic SOX2 mutation and isolated HH... - [Understanding lens differentiation from the analysis of crystallin gene regulation]Masanori Uchikawa
Tanpakushitsu Kakusan Koso 50:655-63. 2005 - Functional analysis of the chicken delta1-crystallin enhancer activity in Drosophila reveals remarkable evolutionary conservation between chicken and flyJorge Blanco
Department of Cell Biology, Biozentrum, University of Basel, Klingelbergstrasse 70, CH 4056 Basel, Switzerland
Development 132:1895-905. 2005..As PAX6 and PAX2 proteins derive from the same ancestor, we propose that during evolution Pax6 function in vertebrate lens development was retained by Pax2 in Drosophila... - Comparative genomics approach to the expression of figalpha, one of the earliest marker genes of oocyte differentiation in medaka (Oryzias latipes)Akira Kanamori
Division of Biological Sciences, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464 8602, Japan
Gene 423:180-7. 2008....