Research Topics
Genomes and Genes | Takahiro OchiyaSummaryAffiliation: National Cancer Center Country: Japan Publications
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Detail Information
Publications
Combined vascular endothelial growth factor-A and fibroblast growth factor 4 gene transfer improves wound healing in diabetic miceAgnieszka Jazwa
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
Genet Vaccines Ther 8:6. 2010....
microRNA as a new immune-regulatory agent in breast milkNobuyoshi Kosaka
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1, Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
Silence 1:7. 2010..Because miRNAs are known to regulate various immune systems, we hypothesized that human breast milk contains miRNAs that may be important for the development of the infant's immune system...
Biomaterials for gene delivery: atelocollagen-mediated controlled release of molecular medicinesT Ochiya
Section for Studies on Metastasis, National Cancer Research Institute, 1 1, Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045, Japan
Curr Gene Ther 1:31-52. 2001....
Commitment of stem cells into functional hepatocytesTakahiro Ochiya
National Cancer Center Research Institute, Tsukiji, Chuoku, Tokyo, Japan
Differentiation 79:65-73. 2010..This paper will review the differentiation ability and mechanisms of these various stem cell types to hepatocytes and their application to liver regeneration and the future outlook...
Rapid hepatic fate specification of adipose-derived stem cells and their therapeutic potential for liver failureAgnieszka Banas
Section for Studies on Metastasis, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
J Gastroenterol Hepatol 24:70-7. 2009..The goal of the present study was to induce human ASC into functional hepatocytes in vitro within a very short period of time and to check their therapeutic potential in vivo...
Combined functional genome survey of therapeutic targets for hepatocellular carcinomaReiko Satow
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan
Clin Cancer Res 16:2518-28. 2010..The outcome of patients with advanced hepatocellular carcinoma (HCC) has remained unsatisfactory. Patients with HCC suffer from chronic hepatitis or liver cirrhosis, and their reserve liver function is often limited...
IFATS collection: in vivo therapeutic potential of human adipose tissue mesenchymal stem cells after transplantation into mice with liver injuryAgnieszka Banas
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Stem Cells 26:2705-12. 2008..Disclosure of potential conflicts of interest is found at the end of this article...
FGF-4 regulates neural progenitor cell proliferation and neuronal differentiationNobuyoshi Kosaka
Department of Biology, School of Education, Waseda University, Tokyo, Japan
FASEB J 20:1484-5. 2006..These findings suggest that FGF-4 has an ability to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain...
Recapitulation of in vivo gene expression during hepatic differentiation from murine embryonic stem cellsYusuke Yamamoto
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Hepatology 42:558-67. 2005..In conclusion, our system closely mimics in vivo hepatic differentiation at the transcriptional level and could, therefore, be useful for studying the molecular basis of hepatocyte differentiation per se...
Direct hepatic fate specification from mouse embryonic stem cellsTakumi Teratani
Section for Studies on Metastasis, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
Hepatology 41:836-46. 2005..Supplementary material for this article can be found on the HEPATOLOGY website ( http://www.interscience.wiley.com/jpages/0270-9139/suppmat/index.html)...
A photon counting technique for quantitatively evaluating progression of peritoneal tumor disseminationKazuyoshi Yanagihara
Central Animal Laboratory, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Cancer Res 66:7532-9. 2006..This animal metastatic model is useful for monitoring the responses of tumors to anticancer agents...
HST-1/FGF-4 protects male germ cells from apoptosis under heat-stress conditionKotaro Hirai
Section for Studies on Metastasis, National Cancer Center Research Institute, Chuo, Tokyo 104 0045, Japan
Exp Cell Res 294:77-85. 2004..These results suggest that HST-1/FGF-4 can act as an important physiological anti-apoptotic factor for male germ cells in stimulating lactate production of Sertoli cells upon heat stress, thereby promoting germ cell survival...
MicroRNA-500 as a potential diagnostic marker for hepatocellular carcinomaYusuke Yamamoto
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045, Japan
Biomarkers 14:529-38. 2009..Our findings reveal that diverse changes of miRNAs occur during liver development and, one of these, miR-500 is an oncofetal miRNA relevant to the diagnosis of human HCC...
RPN2 gene confers docetaxel resistance in breast cancerKimi Honma
Section for Studies on Metastasis, Japanese National Cancer Center Research Institute, 1 1, Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045, Japan
Nat Med 14:939-48. 2008..Thus, RPN2 silencing makes cancer cells hypersensitive response to docetaxel, and RPN2 might be a new target for RNA interference-based therapeutics against drug resistance...
A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cellsYusuke Yamamoto
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo, Japan
FEBS J 275:1260-73. 2008....
Stem cell plasticity: learning from hepatogenic differentiation strategiesAgnieszka Banas
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Dev Dyn 236:3228-41. 2007..We herein present the current knowledge regarding available protocols and factors used in order to obtain functional hepatocytes from stem cells...
Local and systemic delivery of siRNAs for oligonucleotide therapyFumitaka Takeshita
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Methods Mol Biol 487:83-92. 2009..Also, the evaluation of siRNA potency in vivo is central for the selection of therapeutic siRNAs. In this chapter, the effects of atelocollagen-delivered siRNAs in live animals were monitored using bioluminescence imaging...
Stem cells for hepatic regeneration: the role of adipose tissue derived mesenchymal stem cellsTetsuya Ishikawa
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1, Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
Curr Stem Cell Res Ther 5:182-9. 2010..AT-MSC-based therapies may provide a novel approach for hepatic regeneration and hepatocyte differentiation and thereby support hepatic function in diseased individuals...
Generation and hepatic differentiation of human iPS cellsTetsuya Ishikawa
Core Facilities for Research and Innovative Medicine, National Cancer Center Research Institute, Tokyo, Japan
Methods Mol Biol 826:103-14. 2012..Cell Res 19:1233-42, 2009). These cells have potential as patient-specific in vitro models for studying disease etiology and could be used in drug discovery programs tailored to deal with genetic variations in drug efficacy and toxicity...
Efficient delivery of small interfering RNA to bone-metastatic tumors by using atelocollagen in vivoFumitaka Takeshita
Section for Studies on Metastasis, National Cancer Center Research Institute, 1-1 Tsukiji 5-chome, Chuo-ku, Tokyo 104-0045, Japan
Proc Natl Acad Sci U S A 102:12177-82. 2005..Thus, for treatment of bone metastasis of prostate cancer, an atelocollagen-mediated systemic delivery method could be a reliable and safe approach to the achievement of maximal function of siRNA...
Systemic delivery of synthetic microRNA-16 inhibits the growth of metastatic prostate tumors via downregulation of multiple cell-cycle genesFumitaka Takeshita
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Mol Ther 18:181-7. 2010....
Enhanced skin carcinogenesis in cyclin D1-conditional transgenic mice: cyclin D1 alters keratinocyte response to calcium-induced terminal differentiationHanako Yamamoto
National Cancer Center Research Institute, Section for Studies on Metastasis, Tokyo 104 0045, Japan
Cancer Res 62:1641-7. 2002..The conditional transgenic mice described here provide excellent in vivo and in vitro model systems to understand the role of cyclin D1 and deregulation of the cell cycle in carcinogenesis...
"Stem cells into liver"--basic research and potential clinical applicationsAgnieszka Banas
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Adv Exp Med Biol 585:3-17. 2006
Adipose tissue-derived mesenchymal stem cells as a source of human hepatocytesAgnieszka Banas
Section for Studies on Metastasis, National Cancer Center Research Institute 1 1, Tokyo, Japan
Hepatology 46:219-28. 2007..The fact that they are easy to procure ex vivo in large numbers makes them an attractive tool for clinical studies in the context of establishing an alternative therapy for liver dysfunction...
HST-1/FGF-4 plays a critical role in crypt cell survival and facilitates epithelial cell restitution and proliferationHideo Sasaki
Section for Studies on Metastasis, National Cancer Center Research Institute, 1-1 Tsukiji, 5-chome, Chuoku, Tokyo 104-0045, Japan
Oncogene 23:3681-8. 2004....
Hedgehog signal activation in gastric pit cell and in diffuse-type gastric cancerMasahide Fukaya
Genetics Division, National Cancer Center Research Institute, Tsukiji, Tokyo, Japan
Gastroenterology 131:14-29. 2006..The effects of a Hedgehog (Hh) inhibitor, cyclopamine, were assessed in primary gastric epithelium cultures and gastric cancer cell lines...
Secretory mechanisms and intercellular transfer of microRNAs in living cellsNobuyoshi Kosaka
Section for Studies on Metastasis, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
J Biol Chem 285:17442-52. 2010..Our findings shed a ray of light on the physiological relevance of secretory miRNAs...
Long-term maintenance of liver-specific functions in cultured ES cell-derived hepatocytes with hyaluronan spongeTakumi Teratani
National Cancer Center Research Institute, Chuo-ku, Tokyo, Japan
Cell Transplant 14:629-35. 2005..These results demonstrate that the porous HA sponge is an effective support for the in vitro culture of ES-derived hepatocytes used for both basic and applied studies for cell transplantation...
Competitive interactions of cancer cells and normal cells via secretory microRNAsNobuyoshi Kosaka
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
J Biol Chem 287:1397-405. 2012..These results suggest that secretory tumor-suppressive miRNAs can act as a death signal in a cell competitive process. This study provides a novel insight into a tumor initiation mechanism...
[Application of atelocollagen-mediated siRNA delivery for RNAi therapies]Kimi Honma
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Yakugaku Zasshi 127:807-12. 2007..This article reviews the non-viral delivery system of atelocollagen for siRNA, which could be useful for functional screening of the genes in vitro and in vivo, and will provide a foundation for further development of RNAi therapeutics...
Antisense oligodeoxynucleotide against HST-1/FGF-4 suppresses tumorigenicity of an orthotopic model for human germ cell tumor in nude miceKotaro Hirai
Section for Studies on Metastasis, National Cancer Center Research Institute, 1-1, Tsukiji, 5-chome, Chuo-ku, Tokyo 104-0045, Japan
J Gene Med 5:951-7. 2003..The use of Atelocollagen-mediated administration of the antisense HST-1/FGF-4 ODNs may be useful in enhancing the effects of antisense therapy...
Establishment of rat embryonic stem cells and making of chimera ratsShinobu Ueda
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
PLoS ONE 3:e2800. 2008..Most importantly, the rES cells are capable of producing chimera rats. Therefore, we established pluripotent rES cell lines that are widely used to produce genetically modified experimental rats for study of human diseases...
Therapeutic potential of RNA interference against cancerFumitaka Takeshita
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Cancer Sci 97:689-96. 2006..In this review, we present siRNA delivery method strategies and discuss the potential of RNAi-based gene therapy in cancer treatment...
Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiationNobuyoshi Kosaka
Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University, Tokyo, Japan
Br J Haematol 142:293-300. 2008..The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation...
MicroRNA-143 regulates human osteosarcoma metastasis by regulating matrix metalloprotease-13 expressionMitsuhiko Osaki
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan
Mol Ther 19:1123-30. 2011....
Pleiotropic function of FGF-4: its role in development and stem cellsNobuyoshi Kosaka
Section for Studies on Metastasis, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Dev Dyn 238:265-76. 2009..In this review, we focus on the diverse biological functions of FGF-4 in the developmental process and also discuss its putative roles in stem cell biology...
Deficiency of Myo18B in mice results in embryonic lethality with cardiac myofibrillar aberrationsRieko Ajima
Biology Division, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Genes Cells 13:987-99. 2008..5. Thus, Myo18B is a unique unconventional myosin that is predominantly expressed in myocytes and whose expression is essential for the development and/or maintenance of myofibrillar structure...
Versatile applications of microRNA in anti-cancer drug discovery: from therapeutics to biomarkersHaruhisa Iguchi
Section for Studies on Metastasis, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Curr Drug Discov Technol 7:95-105. 2010..Finally, we discuss the potential of miRNAs for therapeutic applications in clinical oncology...
RNAi-based drug discovery and its application to therapeuticsNaomi Hokaiwado
Section for Studies on Metastasis, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
IDrugs 11:274-8. 2008..This feature review focuses on recent progress in the nonviral systemic delivery of siRNA in animal models and in clinical trials, as well as on the application of microRNAs in RNAi therapy...
Differentiation of embryonic stem cells into hepatocytes: biological functions and therapeutic applicationHanako Yamamoto
National Cancer Center Research Institute, Tokyo, Japan
Hepatology 37:983-93. 2003..No teratoma formation was observed in the transplant recipients. In conclusion, ES cells can provide a valuable tool for studying the molecular basis for differentiation of hepatocytes and form the basis for cell therapies...
An integrative genomic analysis revealed the relevance of microRNA and gene expression for drug-resistance in human breast cancer cellsYusuke Yamamoto
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Mol Cancer 10:135. 2011..Here, we performed an integrated genomic analysis combined with array-based comparative hybridization, miRNA, and gene expression microarray to elucidate the mechanism of drug-resistance...
Generation of genetically modified rats from embryonic stem cellsMasaki Kawamata
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045, Japan
Proc Natl Acad Sci U S A 107:14223-8. 2010..Our rat ES cells should be sufficiently able to receive gene targeting as well as Tg manipulation, thus providing valuable animal models for the study of human diseases...
Circulating microRNA in body fluid: a new potential biomarker for cancer diagnosis and prognosisNobuyoshi Kosaka
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 101:2087-92. 2010..In this review, we summarize the usefulness of circulating miRNA for cancer diagnosis, prognosis, and therapeutics. Furthermore, we propose a mechanism for the secretion and incorporation of miRNA into the cells...
Pancreatic endocrine and exocrine cell ontogeny from renal capsule transplanted embryonic stem cells in streptozocin-injured miceMaho Kodama
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045, Japan
J Histochem Cytochem 56:33-44. 2008..The results indicate that beta-cell damage, without overt hyperglycemia, induces a process of fetal-like pancreatogenesis in renal capsule-transplanted ES, leading to beta-cell neogenesis...
Establishment of embryonic stem cells from rat blastocystsMasaki Kawamata
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Methods Mol Biol 597:169-77. 2010..In this chapter, we discuss techniques for the successful establishment and maintenance of rat ES cells...
The role of atelocollagen-based cell transfection array in high-throughput screening of gene functions and in drug discoveryKimi Honma
National Cancer Center Research Institute, 1-1, Tsukiji, 5-chome, Chuo-ku, Tokyo 104-0045, Japan
Curr Drug Discov Technol 1:287-94. 2004..These results suggest that an Atelocollagen-based cell transfection array may be appropriate for general use in the high-throughput screening of large sets of gene medicines with functions in mammalian cells...
Enhanced effects of secreted soluble factor preserve better pluripotent state of embryonic stem cell culture in a membrane-based compartmentalized micro-bioreactorMohammad Mahfuz Chowdhury
Institute of Industrial Science, The University of Tokyo, Tokyo, Japan
Biomed Microdevices 12:1097-105. 2010..The micro-scale culture platform developed in this study enables the investigation of the effects of soluble factors on ESCs in a diffusion dominant microenvironment, and is expected to be used to modulate the ESC fate choices...
[Application of microRNAs to Cancer Therapy]Haruhisa Iguchi
Section for Studies on Metastasis, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Gan To Kagaku Ryoho 37:389-95. 2010..There is growing evidence that miRNA therapy could be a potent means to curtail tumor growth...
MOZ is essential for maintenance of hematopoietic stem cellsTakuo Katsumoto
Molecular Oncology Division, National Cancer Institute, Chuo Ku, Tokyo, Japan
Genes Dev 20:1321-30. 2006..Some aspects of the MOZ-/- phenotype are similar to that observed in PU.1-deficient mice. MOZ was able to interact with PU.1 and activate PU.1-dependent transcription, thus suggesting a physical and functional link between PU.1 and MOZ...
Transgenic rats carrying human c-Ha-ras proto-oncogene are highly susceptible to N-nitrosomethylbenzylamine induction of esophageal tumorigenesisMakoto Asamoto
Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, Chuo-ku, Tokyo 104-0045, Japan
Jpn J Cancer Res 93:744-51. 2002....
Screening of potential molecular targets for colorectal cancer therapyKimi Honma
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan
Int J Gen Med 2:243-57. 2009..Thus, silencing of PSMA7 and RAN induces cancer cells to undergo apoptosis, and PSMA7 and RAN might be promising new molecular targets for drug and RNA interference-based therapeutics against colorectal cancer...
Development and biological analysis of peritoneal metastasis mouse models for human scirrhous stomach cancerKazuyoshi Yanagihara
Central Animal Laboratory, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 96:323-32. 2005....
Gene-manipulated embryonic stem cells for rat transgenesisMasaki Kawamata
Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Cell Mol Life Sci 68:1911-5. 2011..In this review, we will focus on some well-documented examples of gene manipulation in rat ESCs...
Real-time in vivo cellular imaging of graft-versus-host disease and its reaction to immunomodulatory reagentsTakahiro Yamazaki
Division of Gene and Immune Medicine, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Immunol Lett 144:33-40. 2012....
Syngeneic hematopoietic stem cell transplantation enhances the antitumor immunity of intratumoral type I interferon gene transfer for sarcomaTakeshi Udagawa
Division of Gene and Immune Medicine, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Hum Gene Ther 23:173-86. 2012..The treated mice showed no significant adverse events. A combination of intratumoral IFN gene transfer with autologous HSCT could be a promising therapeutic strategy for patients with sarcoma...
HST-1/FGF-4 gene activation induces spermatogenesis and prevents adriamycin-induced testicular toxicityHanako Yamamoto
Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji, 5 Chome, Chuo Ku, Tokyo 104 0045 Japan
Oncogene 21:899-908. 2002..These results with new adenovirus-mediated Cre/lox conditional mice indicated that HST-1/FGF-4 could be an important factor for spermatogenesis, presenting a new paradigm to treat impaired fertility...
Gene expression profiling of cerebellar development with high-throughput functional analysisSakae Saito
Taisho Laboratory of Functional Genomics, Nara Institute of Science and Technology, and Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Tokyo, Japan
Physiol Genomics 22:8-13. 2005..These results demonstrate that functional data can facilitate interpretation of expression profiles and identification of new molecules that participate in biological processes...
