Yasushi Kamisaka

Summary

Affiliation: National Institute of Advanced Industrial Science and Technology
Country: Japan

Publications

  1. ncbi Identification of genes affecting lipid content using transposon mutagenesis in Saccharomyces cerevisiae
    Yasushi Kamisaka
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
    Biosci Biotechnol Biochem 70:646-53. 2006
  2. ncbi Surface plasmon resonance analysis of interactions between diacylglycerol acyltransferase and its interacting molecules
    Yasushi Kamisaka
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 75:1135-9. 2011
  3. ncbi Activation of diacylglycerol acyltransferase expressed in Saccharomyces cerevisiae: overexpression of Dga1p lacking the N-terminal region in the Deltasnf2 disruptant produces a significant increase in its enzyme activity
    Yasushi Kamisaka
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, 305 8566, Japan
    Appl Microbiol Biotechnol 88:105-15. 2010
  4. ncbi DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae
    Yasushi Kamisaka
    Lipid Engineering Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8566, Japan
    Biochem J 408:61-8. 2007
  5. ncbi Production of polyunsaturated fatty acids in yeast Saccharomyces cerevisiae and its relation to alkaline pH tolerance
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Tsukuba, Ibaraki, Japan
    Yeast 26:167-84. 2009
  6. ncbi Improvement of polyunsaturated fatty acids synthesis by the coexpression of CYB5 with desaturase genes in Saccharomyces cerevisiae
    Hisashi Yazawa
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central, Ibaraki, Japan
    Appl Microbiol Biotechnol 87:2185-93. 2010
  7. ncbi Improvement of Stearidonic acid production in Oleaginous Saccharomyces cerevisiae
    Kazuyoshi Kimura
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 73:1447-9. 2009
  8. ncbi Isolation and characterization of a Delta5-desaturase from Oblongichytrium sp
    Yasuyuki Kumon
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 72:2224-7. 2008
  9. ncbi Efficient accumulation of oleic acid in Saccharomyces cerevisiae caused by expression of rat elongase 2 gene (rELO2) and its contribution to tolerance to alcohols
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1 1 1, Tsukuba, Ibaraki, 305 8566, Japan
    Appl Microbiol Biotechnol 91:1593-600. 2011
  10. ncbi Heterologous production of dihomo-gamma-linolenic acid in Saccharomyces cerevisiae
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 73:6965-71. 2007

Collaborators

Detail Information

Publications15

  1. ncbi Identification of genes affecting lipid content using transposon mutagenesis in Saccharomyces cerevisiae
    Yasushi Kamisaka
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Ibaraki, Japan
    Biosci Biotechnol Biochem 70:646-53. 2006
    ..They provide new insight into the biosynthesis of storage lipids in yeast...
  2. ncbi Surface plasmon resonance analysis of interactions between diacylglycerol acyltransferase and its interacting molecules
    Yasushi Kamisaka
    National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 75:1135-9. 2011
    ..Taking all this together, SPR measurement using the Dga1p-immobilized L1 chip surface provided a useful system to elucidate the structure-function relationships of DGAT and screen DGAT inhibitors...
  3. ncbi Activation of diacylglycerol acyltransferase expressed in Saccharomyces cerevisiae: overexpression of Dga1p lacking the N-terminal region in the Deltasnf2 disruptant produces a significant increase in its enzyme activity
    Yasushi Kamisaka
    Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, 305 8566, Japan
    Appl Microbiol Biotechnol 88:105-15. 2010
    ..Dga1DeltaN2p-FLAG(C-terminus) was purified to near homogeneity by anti-FLAG M2 agarose chromatography and maintained significant DGAT activity. These results provide a new strategy to engineer expression of DGAT...
  4. ncbi DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae
    Yasushi Kamisaka
    Lipid Engineering Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology AIST, Tsukuba, Ibaraki 305 8566, Japan
    Biochem J 408:61-8. 2007
    ..The strains obtained have a lipid content that is high enough to act as a model of oleaginous yeast and they may be useful for the metabolic engineering of lipid production in yeast...
  5. ncbi Production of polyunsaturated fatty acids in yeast Saccharomyces cerevisiae and its relation to alkaline pH tolerance
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Tsukuba, Ibaraki, Japan
    Yeast 26:167-84. 2009
    ..The Accession No. for microarray data in the Center for Information Biology Gene Expression database is CBX68...
  6. ncbi Improvement of polyunsaturated fatty acids synthesis by the coexpression of CYB5 with desaturase genes in Saccharomyces cerevisiae
    Hisashi Yazawa
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central, Ibaraki, Japan
    Appl Microbiol Biotechnol 87:2185-93. 2010
    ..Overexpression of cytochrome b5 at 20 degrees C did not affect the fatty acid synthesis so much, but it significantly enhanced the synthesis of PUFA at 30 degrees C...
  7. ncbi Improvement of Stearidonic acid production in Oleaginous Saccharomyces cerevisiae
    Kazuyoshi Kimura
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 73:1447-9. 2009
    ..When this transformed yeast was cultured in the presence of 0.7 g/l alpha-linolenic acid for 7 d, 0.4 g of alpha-linolenic acid was accumulated and 44 mg of stearidonic acid was produced, mainly as a triacylglycerol in the 1 liter broth...
  8. ncbi Isolation and characterization of a Delta5-desaturase from Oblongichytrium sp
    Yasuyuki Kumon
    Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    Biosci Biotechnol Biochem 72:2224-7. 2008
    ..The enzyme had higher activity toward 20:4n-3 than 20:3n-6, indicating that this Delta5-desaturase can be used in the production of n-3 polyunsaturated fatty acids in transgenic organisms...
  9. ncbi Efficient accumulation of oleic acid in Saccharomyces cerevisiae caused by expression of rat elongase 2 gene (rELO2) and its contribution to tolerance to alcohols
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1 1 1, Tsukuba, Ibaraki, 305 8566, Japan
    Appl Microbiol Biotechnol 91:1593-600. 2011
    ..Furthermore, the transformant of rELO2 also conferred tolerance to n-butanol, n-propanol, and 2-propanol...
  10. ncbi Heterologous production of dihomo-gamma-linolenic acid in Saccharomyces cerevisiae
    Hisashi Yazawa
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1 1 1, Tsukuba, Ibaraki 305 8566, Japan
    Appl Environ Microbiol 73:6965-71. 2007
    ..74%. To our knowledge, this is the first report describing the production of polyunsaturated fatty acids in S. cerevisiae without supplying the exogenous fatty acids...
  11. ncbi Appearance of smaller lipid bodies and protein kinase activation in the lipid body fraction are induced by an increase in the nitrogen source in the Mortierella fungus
    Yasushi Kamisaka
    Lipid Engineering Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305 8566
    J Biochem 135:269-76. 2004
    ..Further studies to characterize these protein kinases at the molecular level should provide new insights into the link between nutrient sensing and lipid storage...
  12. ncbi Inhibition of lipid accumulation and lipid body formation in oleaginous yeast by effective components in spices, carvacrol, eugenol, thymol, and piperine
    Kazuyoshi Kimura
    Lipid Engineering Research Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan
    J Agric Food Chem 54:3528-34. 2006
    ....
  13. ncbi Isolation of Delta12 and omega3-fatty acid desaturase genes from the yeast Kluyveromyces lactis and their heterologous expression to produce linoleic and alpha-linolenic acids in Saccharomyces cerevisiae
    Kumiko Kainou
    National Institute of Advanced Industrial Science and Technology, AIST Tsukuba Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan
    Yeast 23:605-12. 2006
    ..The yield of alpha-linolenic acid reached 0.8% of total fatty acids and its production was not increased by adding exogenous oleic acid; alpha-linolenic acid reached 8.7% when exogenous linoleic acid was available...
  14. ncbi Biosynthesis of triacylglycerol molecular species in an oleaginous fungus, Mortierella ramanniana var. angulispora
    Manoj G Pillai
    Lipid Engineering Research Group, Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8566, Japan
    J Biochem (Tokyo) 132:121-6. 2002
    ..This implies that the composition of TG molecular species is regulated through different biosynthetic pathways responsible for specific TG molecular species, providing a new insight into the biosynthesis of TG molecular species...
  15. ncbi Peroxisome proliferator-activated receptor gamma ligands stimulate myeloid differentiation and lipogenensis in human leukemia NB4 cells
    Etsuko Yasugi
    Department of Hematology, Research Institute, International Medical Center of Japan, 1 21 1, Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    Dev Growth Differ 48:177-88. 2006
    ....