Research Topics
Genomes and Genes
| Setsuo HirohashiSummaryAffiliation: National Cancer Center Country: Japan Publications
| Collaborators
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Detail Information
Publications
Cell adhesion system and human cancer morphogenesisSetsuo Hirohashi
Pathology Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
Cancer Sci 94:575-81. 2003..In conclusion, inactivation of the E-cadherin cell adhesion system by both genetic and epigenetic mechanisms plays a significant role during multistage human carcinogenesis...
Chemokine (C-C motif) ligand 2 mediates the prometastatic effect of dysadherin in human breast cancer cellsJeong-Seok Nam
Laboratory of Cell Regulation and Carcinogenesis, National Cancer Institute, Bethesda, MD 20892-5055, USA
Cancer Res 66:7176-84. 2006..This study shows that dysadherin has prometastatic effects that are independent of E-cadherin expression and that CCL2 could play an important role in mediating the prometastatic effect of dysadherin in ER-negative breast cancer...
Morphological and transcriptional responses of untransformed intestinal epithelial cells to an oncogenic beta-catenin proteinYasuyoshi Naishiro
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Oncogene 24:3141-53. 2005..The IGFBP2 protein interacts with integrins. Disruption of the multigene network system regulating cell adhesion and cytoskeleton may be crucial in the initiation of colorectal carcinogenesis...
Combined functional genome survey of therapeutic targets for hepatocellular carcinomaReiko Satow
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan
Clin Cancer Res 16:2518-28. 2010..The outcome of patients with advanced hepatocellular carcinoma (HCC) has remained unsatisfactory. Patients with HCC suffer from chronic hepatitis or liver cirrhosis, and their reserve liver function is often limited...
Orthotopic transplantation models of pancreatic adenocarcinoma derived from cell lines and primary tumors and displaying varying metastatic activityPanayiotis Loukopoulos
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Pancreas 29:193-203. 2004..To establish a series of clinically relevant orthotopic transplantation models of human pancreatic adenocarcinoma from both cell lines and primary tumors under uniform experimental conditions...
Traf2- and Nck-interacting kinase is essential for Wnt signaling and colorectal cancer growthMiki Shitashige
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan
Cancer Res 70:5024-33. 2010..Our findings suggest TNIK as a feasible target for pharmacologic intervention to ablate aberrant Wnt signaling in colorectal cancer...
Krüppel-like factor 12 plays a significant role in poorly differentiated gastric cancer progressionYu Nakamura
Cancer Genomics Project, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Int J Cancer 125:1859-67. 2009..038). These data suggest that the KLF12 gene plays an important role in poorly differentiated gastric cancer progression and is a potential target of therapeutic measures...
Dysadherin expression facilitates cell motility and metastatic potential of human pancreatic cancer cellsTakeshi Shimamura
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Cancer Res 64:6989-95. 2004..Our study suggests that dysadherin is able to modulate actin structures, stimulate cell motility, and contribute directly to the metastatic potential of human pancreatic cancer cells...
Involvement of splicing factor-1 in beta-catenin/T-cell factor-4-mediated gene transactivation and pre-mRNA splicingMiki Shitashige
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 132:1039-54. 2007....
E-cadherin regulates the association between beta-catenin and actinin-4Yasuharu Hayashida
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Cancer Res 65:8836-45. 2005..Shutting down the signals mediating this association may be worth considering as a therapeutic approach to cancer invasion and metastasis...
Genetic clustering of clear cell renal cell carcinoma based on array-comparative genomic hybridization: its association with DNA methylation alteration and patient outcomeEri Arai
Pathology Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
Clin Cancer Res 14:5531-9. 2008..The aim of this study was to clarify genetic and epigenetic alterations occurring during renal carcinogenesis...
Quantitative proteomics using formalin-fixed paraffin-embedded tissues of oral squamous cell carcinomaAyako Negishi
Chemotherapy Division, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Cancer Sci 100:1605-11. 2009....
Transglutaminase 3 as a prognostic biomarker in esophageal cancer revealed by proteomicsNorihisa Uemura
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo, Japan
Int J Cancer 124:2106-15. 2009..These results establish TGM3 as a novel prognostic biomarker for ESCC for the first time. Examination of TGM3 expression may provide novel therapeutic strategies to prevent recurrence of ESCC...
Expression and gene amplification of actinin-4 in invasive ductal carcinoma of the pancreasSatoru Kikuchi
Chemotherapy Division, Cancer Proteomics Project, National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Clin Cancer Res 14:5348-56. 2008..Actinin-4 is an actin-binding protein associated with enhanced cell motility, invasive growth, and lymph node metastasis. Actinin-4 might play an important role in the development and progression of pancreatic cancer...
Genome-wide DNA methylation profiles in liver tissue at the precancerous stage and in hepatocellular carcinomaEri Arai
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Int J Cancer 125:2854-62. 2009..Genome-wide alterations of DNA methylation may participate in hepatocarcinogenesis from the precancerous stage, and DNA methylation profiling may provide optimal indicators for carcinogenetic risk estimation and prognostication...
Prognostic significance of tissue factor in pancreatic ductal adenocarcinomaNobuhiro Nitori
Pathology, National Cancer Center Research Institute, Tokyo, Japan
Clin Cancer Res 11:2531-9. 2005....
Cancer related mutations in NRF2 impair its recognition by Keap1-Cul3 E3 ligase and promote malignancyTatsuhiro Shibata
Cancer Genomics Project, Pathology Division, Center for Medical Genomics, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo, 104 0045, Japan
Proc Natl Acad Sci U S A 105:13568-73. 2008..Congruously, the present study also provides in vivo validation of the two-site substrate recognition model for Nrf2 activation by the Keap1-Cul3-based E3 ligase...
Podoplanin expression identified in stromal fibroblasts as a favorable prognostic marker in patients with colorectal carcinomaTakahiro Yamanashi
Pathology Division, National Cancer Center Research Institute and Hospital, Tokyo, Japan
Oncology 77:53-62. 2009..We investigated the clinicopathological significance of podoplanin expression in stromal fibroblasts in patients with colorectal cancer (CRC)...
Significance of PGP9.5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinomaYuri Akishima-Fukasawa
National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Am J Clin Pathol 134:71-9. 2010..These results indicate that PGP9.5 expression in CAFs is a helpful finding to represent the overall biologic behavior of advanced colorectal cancer...
Genetic classification of lung adenocarcinoma based on array-based comparative genomic hybridization analysis: its association with clinicopathologic featuresTatsuhiro Shibata
Pathology Division, National Cancer Center Research Institute, Tsukjii, Tokyo, Japan
Clin Cancer Res 11:6177-85. 2005....
Genetic profile of hepatocellular carcinoma revealed by array-based comparative genomic hybridization: identification of genetic indicators to predict patient outcomeHiroto Katoh
Pathology Division, National Cancer Center Research Institute, 5-1-1, Tsukiji, Chuo-ku, and Department of Pathology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
J Hepatol 43:863-74. 2005....
Genome-wide array-based comparative genomic hybridization analysis of pancreatic adenocarcinoma: identification of genetic indicators that predict patient outcomePanayiotis Loukopoulos
Pathology Division, National Cancer Center Research Institute, 51 1, Tsukiji, Chuo Ku, Tokyo, USA
Cancer Sci 98:392-400. 2007..Our results contribute to a complete description of genomic structural aberrations and the identification of potential therapeutic targets and genetic indicators that predict patient outcome in pancreatic adenocarcinoma...
Functional interaction of DNA topoisomerase IIalpha with the beta-catenin and T-cell factor-4 complexLin Huang
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 133:1569-78. 2007..DNA topoisomerase IIalpha (Topo IIalpha) is a component of the beta-catenin/T-cell factor-4 (TCF-4) nuclear complex. We examined the functional significance of Topo IIalpha in Wnt signaling...
Molecular markers and changes of computed tomography appearance in lung adenocarcinoma with ground-glass opacityYukihiro Yoshida
Cancer Genomics Project, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Jpn J Clin Oncol 37:907-12. 2007..There is no consensus on the strategy for follow-up. Identification of the molecular mechanisms that are associated with the natural history of lung adenocarcinoma should provide useful information...
Poly(ADP-ribose) polymerase-1 is a component of the oncogenic T-cell factor-4/beta-catenin complexMasashi Idogawa
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 128:1919-36. 2005..We explored the molecular composition of the TCF-4/beta-catenin transcriptional complex by means of proteomics...
Gene expression profiling of epidermal growth factor receptor/KRAS pathway activation in lung adenocarcinomaTatsuhiro Shibata
Cancer Genomics Project, Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 98:985-91. 2007....
Proteomic signatures for histological types of lung cancerMasahiro Seike
Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Proteomics 5:2939-48. 2005..In conclusion, our results demonstrate the utility of quantitative proteomic analysis for molecular diagnosis and classification of lung cancer cells...
Reduced argininosuccinate synthetase is a predictive biomarker for the development of pulmonary metastasis in patients with osteosarcomaEisuke Kobayashi
Chemotherapy Division, National Cancer Centre Research Institute, Chuo Ku, Tokyo, Japan
Mol Cancer Ther 9:535-44. 2010..Our data indicate that in patients with osteosarcoma, reduced expression of ASS is not only a novel predictive biomarker for the development of metastasis, but also a potential target for pharmacologic intervention...
Resequencing and copy number analysis of the human tyrosine kinase gene family in poorly differentiated gastric cancerTakashi Kubo
Cancer Genomics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo, 104 0045, Japan
Carcinogenesis 30:1857-64. 2009..Our focused and integrated analyses of systemic resequencing and gene copy number have revealed the novel onco-kinome profile of GC and pave the way to a comprehensive understanding of the GC genome...
Prolyl 4-hydroxylation of alpha-fibrinogen: a novel protein modification revealed by plasma proteomicsMasaya Ono
Chemotherapy Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
J Biol Chem 284:29041-9. 2009..Prolyl-hydroxylated alpha-fibrinogen is presumably controlled by multiple biological mechanisms, which remain to be clarified in future studies...
Antibody-based proteomics for esophageal cancer: Identification of proteins in the nuclear factor-kappaB pathway and mitotic checkpointNorihisa Uemura
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Cancer Sci 100:1612-22. 2009..The association of these proteins with esophageal cancer may indicate that mitotic checkpoint gene products and NF-kappaB play an important part in the carcinogenesis of esophageal cancer...
Pfetin as a prognostic biomarker in gastrointestinal stromal tumor: novel monoclonal antibody and external validation study in multiple clinical facilitiesKazutaka Kikuta
Proteome Bioinformatics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Jpn J Clin Oncol 40:60-72. 2010..To develop prognostic modalities for GIST patients, we developed a mouse monoclonal antibody against pfetin, the prognostic value of which has been previously reported...
Genome-wide DNA methylation profiles in both precancerous conditions and clear cell renal cell carcinomas are correlated with malignant potential and patient outcomeEri Arai
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Carcinogenesis 30:214-21. 2009..DNA methylation alterations in the precancerous stage may generate more malignant clear cell RCCs and determine patient outcome...
Genetically distinct and clinically relevant classification of hepatocellular carcinoma: putative therapeutic targetsHiroto Katoh
Cancer Genomics Project, National Cancer Center Research Institute, Tokyo, Japan Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 133:1475-86. 2007..Therefore, classification of HCC into genetically homogeneous subclasses would be of great worth to develop novel therapeutic strategies...
Large-scale quantitative clinical proteomics by label-free liquid chromatography and mass spectrometryAyako Negishi
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 100:514-9. 2009..2DICAL was is highly advantageous for large-scale clinical proteomics because of its simple procedure, high throughput, and quantification accuracy...
Pfetin as a prognostic biomarker of gastrointestinal stromal tumors revealed by proteomicsYoshiyuki Suehara
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo, Japan
Clin Cancer Res 14:1707-17. 2008..We aimed to develop prognostic biomarkers for gastrointestinal stromal tumors (GIST) using a proteomic approach...
EBP50, a beta-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinomaTatsuhiro Shibata
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Hepatology 38:178-86. 2003..In conclusion, the EBP50/beta-catenin complex promotes Wnt signaling, and over-expression of EBP50 may work cooperatively with beta-catenin in the development of liver cancer...
Resequencing analysis of the human tyrosine kinase gene family in pancreatic cancerTakashi Kubo
National Cancer Center Research Institute, Chuo Ku, Tokyo, Japan
Pancreas 38:e200-6. 2009..The tyrosine kinase (TK) gene family, which encodes important regulators of various signal transduction pathways, is one of the most frequently altered gene families in human cancer...
Actinin-4 increases cell motility and promotes lymph node metastasis of colorectal cancerKazufumi Honda
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuoh ku, Tokyo 104 0045, Japan
Gastroenterology 128:51-62. 2005..However, its involvement in the mechanisms of cancer invasion and metastasis remains undetermined. The current study tested the role of actinin-4 in the motility and metastatic potential of colorectal cancer cells...
Overexpression of cortactin is involved in motility and metastasis of hepatocellular carcinomaMakoto Chuma
Pathology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
J Hepatol 41:629-36. 2004..The molecular basis of the metastasis of hepatocellular carcinoma (HCC) is not fully understood. The aim of this study was to elucidate the crucial genes involved in metastasis of HCC...
Clinicopathologic significance of dysadherin expression in cutaneous malignant melanoma: immunohistochemical analysis of 115 patientsAya Nishizawa
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Cancer 103:1693-700. 2005..Recently, the authors reported a novel cell membrane glycoprotein, dysadherin, which has an anti-cell-cell adhesion function and down-regulates E-cadherin...
Regulation of Wnt signaling by the nuclear pore complexMiki Shitashige
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 134:1961-71, 1971.e1-4. 2008..However, beta-catenin has no nuclear localization signal, and the mechanisms by which beta-catenin is imported into the nucleus and forms a complex with the TCF-4 nuclear protein are poorly understood...
Plasma proteomics of pancreatic cancer patients by multi-dimensional liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE): up-regulation of leucine-rich alpha-2-glycoprotein in pancreatic cancerTatsuhiko Kakisaka
Proteome Bioinformatics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
J Chromatogr B Analyt Technol Biomed Life Sci 852:257-67. 2007..Our results demonstrate the application of a combination of multi-dimensional liquid chromatography with 2D-DIGE for plasma proteomics and suggest the clinical utility of LRG plasma level measurement...
Genetic alteration of Keap1 confers constitutive Nrf2 activation and resistance to chemotherapy in gallbladder cancerTatsuhiro Shibata
Cancer Genomics Project, National Cancer Center Research Institute, Tokyo, Japan Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Gastroenterology 135:1358-1368, 1368.e1-4. 2008..We attempted to examine whether deregulation of Nrf2, a master transcriptional factor of various cytoprotective genes against oxidative stress, plays a role in the carcinogenesis of BTC...
Database of two-dimensional polyacrylamide gel electrophoresis of proteins labeled with CyDye DIGE Fluor saturation dyeKazuyasu Fujii
Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Proteomics 6:1640-53. 2006....
Genome-wide DNA methylation profiles in renal tumors of various histological subtypes and non-tumorous renal tissuesEri Arai
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Pathobiology 78:1-9. 2011..The aim of this study is to clarify genome-wide DNA methylation profiles in renal tumors of various histological subtypes...
Genetic inactivation of the APC gene contributes to the malignant progression of sporadic hepatocellular carcinoma: a case reportHiroto Katoh
Cancer Genomics Project, National Cancer Center Research Institute, Tsukiji, Tokyo, Japan
Genes Chromosomes Cancer 45:1050-7. 2006....
Label-free quantitative proteomics using large peptide data sets generated by nanoflow liquid chromatography and mass spectrometryMasaya Ono
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Mol Cell Proteomics 5:1338-47. 2006..39 and correlation coefficient of over 0.92 between duplicates), and accurate quantification with a wide dynamic range (over 10(3)) of our platform warrant its application to various types of experimental and translational proteomics...
Laminin 5 beta3 and gamma2 chains are frequently coexpressed in cancer cellsShingo Akimoto
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Pathol Int 54:688-92. 2004..0001). These results suggest that in squamous cell carcinoma of the tongue and colorectal carcinoma, laminin 5 gamma2 chain and beta3 chain are both important in the invasiveness of cancer cells...
Beta-catenin mutations in pulmonary blastomas: association with morule formationShigeki Sekine
Pathology Division, National Cancer Center Research Institute and Hospital, Tokyo, Japan
J Pathol 200:214-21. 2003..The presence of this common genetic alteration found in both WDFA and BPB implies a histogenetic linkage between these tumours...
Expression profiling in hepatocellular carcinoma with intrahepatic metastasis: identification of high-mobility group I(Y) protein as a molecular marker of hepatocellular carcinoma metastasisMakoto Chuma
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Keio J Med 53:90-7. 2004..HMG-I(Y) expression was associated with intrahepatic metastasis and may be a predictive marker of HCC intrahepatic metastasis...
Development and biological analysis of peritoneal metastasis mouse models for human scirrhous stomach cancerKazuyoshi Yanagihara
Central Animal Laboratory, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 96:323-32. 2005....
Expression profiling in ovarian clear cell carcinoma: identification of hepatocyte nuclear factor-1 beta as a molecular marker and a possible molecular target for therapy of ovarian clear cell carcinomaAkira Tsuchiya
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Am J Pathol 163:2503-12. 2003..Our results suggest that HNF-1 beta is not only an excellent CCC-specific molecular marker but also a molecular target for therapy of ovarian CCC...
Cytoplasmic p120ctn regulates the invasive phenotypes of E-cadherin-deficient breast cancerTatsuhiro Shibata
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Am J Pathol 164:2269-78. 2004..Collectively, our results suggest that cytoplasmic p120ctn may contribute to the invasive phenotype of E-cadherin-deficient breast cancer cells...
Expression of liver-intestine cadherin and its possible interaction with galectin-3 in ductal adenocarcinoma of the pancreasMasaaki Takamura
Pathology Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
Cancer Sci 94:425-30. 2003....
Dysadherin overexpression in pancreatic ductal adenocarcinoma reflects tumor aggressiveness: relationship to e-cadherin expressionTakeshi Shimamura
Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
J Clin Oncol 21:659-67. 2003..CONCLUSION: In pancreatic ductal adenocarcinoma, dysadherin expression seems to reflect tumor aggressiveness and to be a positive marker of poor prognosis when considered both alone and in combination with downregulation of E-cadherin...
Alternative splice variant of actinin-4 in small cell lung cancerKazufumi Honda
Cancer Proteomics Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Oncogene 23:5257-62. 2004..The variant protein showed high affinity to filamentous actin polymers and was not localized with cortical actin. Alternatively spliced actinin-4 may be a new diagnostic marker of SCLC and a candidate target for selective therapy...
Protein expression associated with early intrahepatic recurrence of hepatocellular carcinoma after curative surgeryHideki Yokoo
Proteome Bioinformatics Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo ku Tokyo 104 0045, Japan
Cancer Sci 98:665-73. 2007..The identified proteins may be candidates for prognostic markers and contribute to the improvement of existing therapeutic strategies...
5-aza-2'-deoxycytidine restores the E-cadherin system in E-cadherin-silenced cancer cells and reduces cancer metastasisJeong-Seok Nam
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Clin Exp Metastasis 21:49-56. 2004..Therefore, inhibition of DNA methylation may be useful for preventing cancer metastasis...
Array-based comparative genomic hybridization analysis of high-grade neuroendocrine tumors of the lungWei-Xia Peng
Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Cancer Sci 96:661-7. 2005..These results will be valuable for evaluating clinical status, including patient prognosis, and for identifying novel molecular targets for effective therapies...
Akt phosphorylation is a risk factor for early disease recurrence and poor prognosis in hepatocellular carcinomaKazuaki Nakanishi
Division of Pathology, National Cancer Center Research Institute, Tokyo, Japan
Cancer 103:307-12. 2005..05). CONCLUSIONS: The current clinical study showed the critical involvement of Akt phosphorylation in the aggressiveness of HCC. The potential benefits of surgery should be assessed carefully in patients with any of these risk factors...
Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomicsKazutaka Kikuta
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tokyo, Japan
Proteomics Clin Appl 4:560-7. 2010..We aimed to identify novel chemotherapy responsiveness biomarkers for osteosarcoma (OS) by investigating the global protein expression profile of 12 biopsy samples from OS patients...
Molecular background of alpha-fetoprotein in liver cancer cells as revealed by global RNA expression analysisShigeru Saito
Proteome Bioinformatics Project, National Cancer Center Research Institute, Tsukiji, Chuo Ku, Tokyo, Japan
Cancer Sci 99:2402-9. 2008..From this genome-wide association study, novel aspects of the molecular background of AFP were revealed, and thus may lead to the identification of novel biomarker candidates...
Dysadherin, a cancer-associated cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasisYoshinori Ino
Pathology Division, National Cancer Center Research Institute, 5 1 1, Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Proc Natl Acad Sci U S A 99:365-70. 2002..We have named this cell membrane glycoprotein, which down-regulates E-cadherin and promotes metastasis, dysadherin...
Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutationsYoshiya Yamamoto
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan Third Department of Internal Medicine, Hokkaido University Faculty of Medicine, Sapporo, Japan
Hepatology 37:528-33. 2003..In conclusion, although much is still unknown, Gpr49 may be critically involved in the development of HCCs with beta-catenin mutations and has the potential to be a new therapeutic target in the treatment of HCC...
Increased DNA methyltransferase 1 (DNMT1) protein expression in precancerous conditions and ductal carcinomas of the pancreasDun Fa Peng
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Cancer Sci 96:403-8. 2005....
Src family kinase inhibitor PP2 restores the E-cadherin/catenin cell adhesion system in human cancer cells and reduces cancer metastasisJeong-Seok Nam
Pathology Division, National Cancer Center Research Institute, Tokyo 104-0045, Japan
Clin Cancer Res 8:2430-6. 2002..Thus, selective inhibition of Src activation may be potentially useful in the prevention of cancer metastasis...
Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesisYoshimasa Saito
Pathology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Proc Natl Acad Sci U S A 99:10060-5. 2002....
Critical involvement of the phosphatidylinositol 3-kinase/Akt pathway in anchorage-independent growth and hematogeneous intrahepatic metastasis of liver cancerKazuaki Nakanishi
Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Cancer Res 62:2971-5. 2002..These findings indicated that anchorage-independent growth regulated by phosphatidylinositol 3-kinase/Akt pathway plays a critical role in metastasis, and that this could be a potential therapeutic target to combat metastasis...
Target disruption of the mutant beta-catenin gene in colon cancer cell line HCT116: preservation of its malignant phenotypeShigeki Sekine
Pathology Division, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Oncogene 21:5906-11. 2002..Although beta-catenin stabilization affects some biological characteristics including adhesive properties, it may not have growth-promoting effects at least in some colorectal carcinomas...
Frequent co-localization of Cox-2 and laminin-5 gamma2 chain at the invasive front of early-stage lung adenocarcinomasToshiro Niki
Pathology and Biology Division, National Cancer Center Research Institute, Tokyo, Japan
Am J Pathol 160:1129-41. 2002..Further investigations are warranted concerning the role of cox-2 and laminin-5 in cancer cell invasion and the significance of p53 and EGFR signaling in the regulation of cox-2 and laminin-5 expression...
Proteomic analysis of intestinal epithelial cells expressing stabilized beta-cateninMasahiro Seike
Cancer Proteomics Project, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
Cancer Res 63:4641-7. 2003..These results suggest that aberrant accumulation of beta-catenin might contribute to colorectal carcinogenesis by affecting redox status in the mitochondria of intestinal epithelial cells...
Distinct gene expression-defined classes of gastrointestinal stromal tumorUmio Yamaguchi
Chemotherapy Division and Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo 104 0045, Japan
J Clin Oncol 26:4100-8. 2008..GISTs are considered to invariably arise through gain of function KIT or PDGFA mutation of the interstitial cells of Cajal (ICC). However, the genetic basis of the malignant progression of GISTs are poorly understood...
Increased DNA methyltransferase 1 (DNMT1) protein expression correlates significantly with poorer tumor differentiation and frequent DNA hypermethylation of multiple CpG islands in gastric cancersTsuyoshi Etoh
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Am J Pathol 164:689-99. 2004..Furthermore, DNMT1 may play a significant role in the development of poorly differentiated gastric cancers by inducing frequent DNA hypermethylation of multiple CpG islands...
Tyrosine-phosphorylation of the 12th armadillo-repeat of beta-catenin is associated with cadherin dysfunction in human cancerKohei Shomori
Pathology Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo, Japan
Int J Oncol 35:517-24. 2009..These findings indicated that the tyrosine phosphorylation of arm12 is a key marker of cadherin dysfunction and the 4G7 antibody may be useful in screening for a beta-catenin phosphorylation ligand or tyrosine kinases...
Identification of a predictive biomarker for hematologic toxicities of gemcitabineJunichi Matsubara
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan
J Clin Oncol 27:2261-8. 2009..This study aimed to discover a new diagnostic method for predicting the hematologic toxicities of gemcitabine...
Heterogeneity in the modification and involvement of chromatin components of the CpG island of the silenced human CDH1 gene in cancer cellsShiro Koizume
DNA Methylation and Genome Function Project and Pathology Division, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo 104 0045, Japan
Nucleic Acids Res 30:4770-80. 2002..These results demonstrate that chromatin components associated with inactive CDH1 chromatin is heterogeneously modified and suggests the presence of multiple pathways for the formation of inactive chromatin...
Regional DNA hypermethylation and DNA methyltransferase (DNMT) 1 protein overexpression in both renal tumors and corresponding nontumorous renal tissuesEri Arai
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
Int J Cancer 119:288-96. 2006..Regional DNA hypermethylation may be associated with renal tumorigenesis from a precancerous condition to malignant progression and become a predictor of patient prognosis...
Proteomic signature of human cancer cellsMasahiro Seike
Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo, Japan
Proteomics 4:2776-88. 2004....
Application of 2D-DIGE in cancer proteomics toward personalized medicineTadashi Kondo
National Cancer Center Research Institute, Proteome Bioinformatics Project, Tokyo, Japan
Methods Mol Biol 577:135-54. 2009..Here we demonstrate the advantages of 2D-DIGE and discuss the possibilities 2D-DIGE offers for further, more comprehensive proteome studies...
Ku70 and poly(ADP-ribose) polymerase-1 competitively regulate beta-catenin and T-cell factor-4-mediated gene transactivation: possible linkage of DNA damage recognition and Wnt signalingMasashi Idogawa
Chemotherapy Division and ADP Ribosylation in Oncology Project, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Cancer Res 67:911-8. 2007..Identification of the functional interaction of Ku70 as well as PARP-1 with the TCF-4 and beta-catenin transcriptional complex may provide insights into a novel linkage between DNA damage recognition/repair and Wnt signaling...
Prevalence of FOXP3+ regulatory T cells increases during the progression of pancreatic ductal adenocarcinoma and its premalignant lesionsNobuyoshi Hiraoka
Pathology Division, National Cancer Center Research Institute and Division of Hepatobiliary and Pancreatic Surgery, National Cancer Center Central Hospital, Tokyo, Japan
Clin Cancer Res 12:5423-34. 2006..The aim of this study was to investigate the clinical significance and roles of TR in the progression and multistep carcinogenesis of pancreatic ductal adenocarcinoma...
Alterations in gene expression associated with the overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, during human hepatocarcinogenesisYae Kanai
Pathology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, 104 0045, Tokyo, Japan
J Cancer Res Clin Oncol 130:636-44. 2004..We aimed to elucidate further the significance of DNMT3b4 during human hepatocarcinogenesis...
Establishment of two cell lines from human gastric scirrhous carcinoma that possess the potential to metastasize spontaneously in nude miceKazuyoshi Yanagihara
Central Animal Laboratory, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
Cancer Sci 95:575-82. 2004..This is the first report of cultured scirrhous gastric carcinoma cells showing the potential for spontaneous metastasis...
Possible involvement of tetraspanin CO-029 in hematogenous intrahepatic metastasis of liver cancer cellsKengo Kanetaka
Pathology Division, National Cancer Center Research Institute, Tokyo, Japan
J Gastroenterol Hepatol 18:1309-14. 2003..CONCLUSION: These results suggest that CO-029 might be involved in hematogenous intrahepatic metastasis, although the precise cellular mechanisms involved remain unknown...
Reduced plasma level of CXC chemokine ligand 7 in patients with pancreatic cancerJunichi Matsubara
Chemotherapy Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Cancer Epidemiol Biomarkers Prev 20:160-71. 2011..Early detection is essential to improve the outcome of patients with pancreatic cancer. A noninvasive and cost-effective diagnostic test using plasma/serum biomarkers would facilitate the detection of pancreatic cancer at the early stage...
Ras farnesylation inhibitor FTI-277 restores the E-cadherin/catenin cell adhesion system in human cancer cells and reduces cancer metastasisJeong Seok Nam
Pathology Division, National Cancer Center Research Institute, Chuo Ku, Tokyo 104 0045, Japan
Jpn J Cancer Res 93:1020-8. 2002..Therefore, selective inhibition of Ras activation may be useful for preventing cancer metastasis...
Establishment of perineural invasion models and analysis of gene expression revealed an invariant chain (CD74) as a possible molecule involved in perineural invasion in pancreatic cancerNorimasa Koide
Departments of Pathology and Surgery, School of Medicine, Keio University, USA
Clin Cancer Res 12:2419-26. 2006..In combination with gene expression profiling, it was indicated that CD74 could be a candidate molecule involved in perineural invasion. These models provide new approaches for study of perineural invasion in pancreatic cancer...
Prognostic significance of dysadherin expression in esophageal squamous cell carcinomaYutaka Shimada
Department of Surgery and Surgical Basic Science, Graduate School of Medicine, Kyoto University, Kawaracho, Shogoin Sakyo ku, Japan
Oncology 67:73-80. 2004..To evaluate the role of dysadherin in metastasis of esophageal squamous cell carcinoma (ESCC), we examined dysadherin and E-cadherin expression in patients with this cancer...
Dysadherin expression as a significant prognostic factor and as a determinant of histologic features in synovial sarcoma: special reference to its inverse relationship with E-cadherin expressionTeiyu Izumi
Department of Anatomic Pathology, Pathological Sciences, Graduate School of Medical Sciences, Kyushu University, Maidashi 3 1 1, Higashi ku, Fukuoka 812 8582, Japan
Am J Surg Pathol 31:85-94. 2007..Dysadherin expression is considered to be one of the determinants of histologic subtype in synovial sarcoma. Moreover, dysadherin expression is an excellent and independent prognostic indicator...
Frequent silencing of the candidate tumor suppressor PCDH20 by epigenetic mechanism in non-small-cell lung cancersIssei Imoto
Department of Molecular Cytogenetics, Medical Research Institute and Graduate School of Biomedical Science, Tokyo Medical and Dental University, Tokyo, Japan
Cancer Res 66:4617-26. 2006..These results suggest that epigenetic silencing by hypermethylation of the CpG-rich promoter region of PCDH20 leads to loss of PCDH20 function, which may be a factor in the carcinogenesis of NSCLC...
Motility enhancement by tumor-derived mutant E-cadherin is sensitive to treatment with epidermal growth factor receptor and phosphatidylinositol 3-kinase inhibitorsMargit Fuchs
Klinikum rechts der Isar, Institut für allgemeine Pathologie und pathologische Anatomie, Technische Universitat Munchen, Munich, Germany
Exp Cell Res 276:129-41. 2002..Instead, the basal level of PI 3-kinase is necessary for mutant E-cadherin-enhanced cell motility. Our data suggest a critical role of E-cadherin mutations for the fine tuning of tumor cell motility...
ADAM23, a possible tumor suppressor gene, is frequently silenced in gastric cancers by homozygous deletion or aberrant promoter hypermethylationHisashi Takada
Department of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Bunkyo ku, Tokyo, Japan
Oncogene 24:8051-60. 2005..These results suggest that genetic or epigenetic silencing by hypermethylation of the ADAM23 CpG-rich promoter region leads to loss of ADAM23 function, which may be a factor in gastric carcinogenesis...
Genetic variation in PSCA is associated with susceptibility to diffuse-type gastric cancerHiromi Sakamoto
Nat Genet 40:730-40. 2008..90, 95% CI = 1.56-2.33, P = 8.01 x 10(-11)). The polymorphism of the PSCA gene, which is possibly involved in regulating gastric epithelial-cell proliferation, influences susceptibility to diffuse-type gastric cancer...
Loss of Keap1 function activates Nrf2 and provides advantages for lung cancer cell growthTsutomu Ohta
Center for Medical Genomics, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Cancer Res 68:1303-9. 2008..Thus, inhibition of NRF2 may provide new therapeutic approaches in lung cancers with activation of Nrf2...
Frequent silencing of low density lipoprotein receptor-related protein 1B (LRP1B) expression by genetic and epigenetic mechanisms in esophageal squamous cell carcinomaItaru Sonoda
Department of Molecular Cytogenetics, Medical Research Institute, and Maxillofacial Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan
Cancer Res 64:3741-7. 2004..These results suggest that loss of LRP1B function in esophageal carcinogenesis most often occurs either by homozygous deletion or by transcriptional silencing through hypermethylation of its CpG island...
Frequent inactivation of a putative tumor suppressor, angiopoietin-like protein 2, in ovarian cancerRyoko Kikuchi
Department of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Tokyo, Japan
Cancer Res 68:5067-75. 2008..These results suggest that, at least partly, epigenetic silencing by hypermethylation of the ANGPTL2 promoter leads to a loss of ANGPTL2 function, which may be a factor in the carcinogenesis of OC in a stage-dependent manner...
Alterations of DNA methylation associated with abnormalities of DNA methyltransferases in human cancers during transition from a precancerous to a malignant stateYae Kanai
Pathology Division, National Cancer Center Research Institute, 5 1 1 Tsukiji, Chuo Ku, Tokyo 104 0045, Japan
Carcinogenesis 28:2434-42. 2007..Correction of DNA methylation status may offer a new strategy for prevention and therapy of cancers...
Increased expression of the LGALS3 (galectin 3) gene in human non-small-cell lung cancerAkinobu Yoshimura
Fourth Department of Internal Medicine, Nippon Medical School, Tokyo, Japan
Genes Chromosomes Cancer 37:159-64. 2003..Accordingly, LGALS3 may be a phenotypic marker that excludes small-cell lung cancer and may represent a novel target molecule in non-small-cell lung cancer therapy...
Promoter hypermethylation contributes to frequent inactivation of a putative conditional tumor suppressor gene connective tissue growth factor in ovarian cancerRyoko Kikuchi
Department of Molecular Cytogenetics, Medical Research Institute and School of Biomedical Science, Tokyo Medical and Dental University, Japan
Cancer Res 67:7095-105. 2007....
Suppression of intestinal polyposis in Mdr1-deficient ApcMin/+ miceTesshi Yamada
Cancer Proteomics Project, National Cancer Center Research Institute, Tokyo 104 0045, Japan
Cancer Res 63:895-901. 2003..Inhibitors of P-glycoprotein may be included in a novel class of chemopreventive agents against colorectal carcinogenesis...
