Yuki Naito

Summary

Country: Japan

Publications

  1. ncbi siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    BMC Bioinformatics 10:392. 2009
  2. ncbi Optimal design and validation of antiviral siRNA for targeting HIV-1
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Retrovirology 4:80. 2007
  3. ncbi dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Nucleic Acids Res 33:W589-91. 2005
  4. ncbi [Designing functional siRNA sequences]
    Tomoyuki Yamada
    Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
    Seikagaku 77:1519-25. 2005
  5. ncbi siVirus: web-based antiviral siRNA design software for highly divergent viral sequences
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Nucleic Acids Res 34:W448-50. 2006
  6. ncbi Guidelines for the selection of effective short-interfering RNA sequences for functional genomics
    Kumiko Ui Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Methods Mol Biol 361:201-16. 2007
  7. ncbi Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    Nucleic Acids Res 36:2136-51. 2008
  8. ncbi Thermodynamic stability and Watson-Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Nucleic Acids Res 36:7100-9. 2008
  9. ncbi siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 32:W124-9. 2004
  10. ncbi Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, School of Science, University of Tokyo, Japan
    Nucleic Acids Res 32:936-48. 2004

Collaborators

Detail Information

Publications11

  1. ncbi siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    BMC Bioinformatics 10:392. 2009
    ..Thus, an efficient algorithm for designing functional siRNAs with minimal off-target effect based on the mechanistic features is considered of value...
  2. ncbi Optimal design and validation of antiviral siRNA for targeting HIV-1
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan
    Retrovirology 4:80. 2007
    ..We present several promising antiviral siRNA candidates that effectively inhibited multiple subtypes of HIV-1 by targeting the best conserved regions in pandemic HIV-1 group M strains...
  3. ncbi dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
    Nucleic Acids Res 33:W589-91. 2005
    ....
  4. ncbi [Designing functional siRNA sequences]
    Tomoyuki Yamada
    Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
    Seikagaku 77:1519-25. 2005
  5. ncbi siVirus: web-based antiviral siRNA design software for highly divergent viral sequences
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Nucleic Acids Res 34:W448-50. 2006
    ....
  6. ncbi Guidelines for the selection of effective short-interfering RNA sequences for functional genomics
    Kumiko Ui Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Methods Mol Biol 361:201-16. 2007
    ....
  7. ncbi Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    Nucleic Acids Res 36:2136-51. 2008
    ..Thus, it may be possible to design DNA-RNA chimeras which effectively silence mammalian target genes without silencing unintended genes...
  8. ncbi Thermodynamic stability and Watson-Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Nucleic Acids Res 36:7100-9. 2008
    ..The off-target effect was more sensitive to siRNA concentration compared to intended gene silencing, which requires a near perfect sequence match between the siRNA guide strand and target mRNA...
  9. ncbi siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference
    Yuki Naito
    Department of Biophysics and Biochemistry, Graduate School of Science, School of Science, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 32:W124-9. 2004
    ..The website accepts an arbitrary sequence as input and quickly returns siRNA candidates, providing a wide scope of applications in mammalian RNAi, including systematic functional genomics and therapeutic gene silencing...
  10. ncbi Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference
    Kumiko Ui-Tei
    Department of Biophysics and Biochemistry, Graduate School of Science, School of Science, University of Tokyo, Japan
    Nucleic Acids Res 32:936-48. 2004
    ..In contrast to mammalian and chick cells, little siRNA sequence preference could be detected in Drosophila in vivo RNAi...
  11. ncbi E-cadherin is transcriptionally activated via suppression of ZEB1 transcriptional repressor by small RNA-mediated gene silencing
    Minami Mazda
    Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan
    PLoS ONE 6:e28688. 2011
    ..Thus, E-cadherin could be upregulated by the suppression of ZEB1 transcriptional repressor by miRNAs in vivo...