A Murasugi

Summary

Affiliation: Meiji Dairies Corporation
Country: Japan

Publications

  1. ncbi Production of recombinant human bile salt-stimulated lipase in Pichia pastoris
    A Murasugi
    Meiji Institute of Health Science, Meiji Milk Products Company Ltd, 540 Naruda, Odawara, Kanagawa 250 0862, Japan
    Protein Expr Purif 23:282-8. 2001
  2. ncbi Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris
    A Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 65:2291-3. 2001
  3. ncbi Characterization of partially truncated human midkine expressed in Pichia pastoris
    Akira Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 66:1295-300. 2002
  4. ncbi Production of native recombinant human midkine in the yeast, Pichia pastoris
    Akira Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, 540 Naruda, Odawara, Kanagawa 250 0862, Japan
    Protein Expr Purif 27:244-52. 2003
  5. ncbi Efficient production of recombinant human pleiotrophin in yeast, Pichia pastoris
    Akira Murasugi
    Meiji Institute of Health Science, Meiji Milk Products Co, Ltd, Naruda, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 67:2288-90. 2003
  6. ncbi Secretory expression of human protein in the Yeast Pichia pastoris by controlled fermentor culture
    Akira Murasugi
    Product Development Department, Meiji Dairies Corporation, 1 2 10, Shin suna, Koto ku, Tokyo 136 8908, Japan
    Recent Pat Biotechnol 4:153-66. 2010

Detail Information

Publications6

  1. ncbi Production of recombinant human bile salt-stimulated lipase in Pichia pastoris
    A Murasugi
    Meiji Institute of Health Science, Meiji Milk Products Company Ltd, 540 Naruda, Odawara, Kanagawa 250 0862, Japan
    Protein Expr Purif 23:282-8. 2001
    ..The secreted rhBSSL was efficiently captured and purified by expanded bed adsorption chromatography...
  2. ncbi Comparison of three signals for secretory expression of recombinant human midkine in Pichia pastoris
    A Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 65:2291-3. 2001
    ..pastoris. Approximately 28 mg/l, 1.5 mg/l, and 0.2 mg/l of midkine were secreted by using the a mating factor pre-pro-sequence, the midkine signal sequence, and the phosphatase signal sequence in flask cultures, respectively...
  3. ncbi Characterization of partially truncated human midkine expressed in Pichia pastoris
    Akira Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 66:1295-300. 2002
    ..The other ones lost 5-12 amino acid residues from the amino-termini, provably by proteolysis. Even the mixture of these truncated midkines could promote CHO cell proliferation as well as the authentic rh-midkine...
  4. ncbi Production of native recombinant human midkine in the yeast, Pichia pastoris
    Akira Murasugi
    Meiji Cell Technology Center, Meiji Milk Products Co, Ltd, 540 Naruda, Odawara, Kanagawa 250 0862, Japan
    Protein Expr Purif 27:244-52. 2003
    ..The circular dichroic spectrum indicated only a negative Cotton effect at 215 nm. Only beta-structures were indicated for the rh-midkine molecule in solution. Purified rh-midkine was active in a cell-proliferation assay...
  5. ncbi Efficient production of recombinant human pleiotrophin in yeast, Pichia pastoris
    Akira Murasugi
    Meiji Institute of Health Science, Meiji Milk Products Co, Ltd, Naruda, Odawara, Kanagawa, Japan
    Biosci Biotechnol Biochem 67:2288-90. 2003
    ..In the purified rhPTN preparation, modified rhPTN were scarcely detected. Circular dichroism measurement of the purified PTN showed the presence of the characteristic beta-structures in the protein...
  6. ncbi Secretory expression of human protein in the Yeast Pichia pastoris by controlled fermentor culture
    Akira Murasugi
    Product Development Department, Meiji Dairies Corporation, 1 2 10, Shin suna, Koto ku, Tokyo 136 8908, Japan
    Recent Pat Biotechnol 4:153-66. 2010
    ..When humanization of Pichia glycans is established by genetic engineering, the Pichia pastoris expression system will become indispensable for the production of therapeutic proteins...