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Genomes and Genes | Hiroya YurimotoSummaryAffiliation: Kyoto University Country: Japan Publications
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Publications
HxlR, a member of the DUF24 protein family, is a DNA-binding protein that acts as a positive regulator of the formaldehyde-inducible hxlAB operon in Bacillus subtilisHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
Mol Microbiol 57:511-9. 2005..subtilis. Thus, we have shown that HxlR is a DNA-binding protein that is necessary for formaldehyde-induced expression of hxlAB in B. subtilis...- Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolismHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
Chem Rec 5:367-75. 2005..Regulation of the genes encoding these enzymes and their catalytic mechanisms depend on the physiological traits of these organisms during evolution... - Molecular basis of methanol-inducible gene expression and its application in the methylotrophic yeast Candida boidiniiHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
Biosci Biotechnol Biochem 73:793-800. 2009..boidinii. Application of this gained knowledge led to successful production of useful enzymes in this yeast, which is also reviewed... - Methanol-inducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidiniiHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
Biotechnol Appl Biochem 53:85-92. 2009..boidinii. In addition, the utility of C. boidinii gene expression systems for the production of various enzymes is also reviewed... - Genomic organization and biochemistry of the ribulose monophosphate pathway and its application in biotechnologyHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, 606 8502, Japan
Appl Microbiol Biotechnol 84:407-16. 2009..Heterologous expression of HPS and PHI in various organisms allows them to metabolize and detoxify formaldehyde, and we also review recent progress in such applications in biotechnology... - Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidiniiYu Sasano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, Japan
FEMS Yeast Res 10:535-44. 2010..These results suggest that Trm2p-dependent derepression is essential for the Trm1p-dependent methanol-specific gene activation in C. boidinii... - Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomeraseIzumi Orita
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, 606 8502 Kyoto, Japan
Appl Microbiol Biotechnol 76:439-45. 2007..Our results demonstrate that the engineered fusion gene has the possibility to be used to establish a formaldehyde-resistance detoxification system in various organisms... - Novel acetone metabolism in a propane-utilizing bacterium, Gordonia sp. strain TY-5Tetsuya Kotani
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Kyoto 606 8502, Japan
J Bacteriol 189:886-93. 2007..This paper provides the first evidence for BVMO-dependent acetone metabolism... - Methylovulum miyakonense gen. nov., sp. nov., a type I methanotroph isolated from forest soilHiroyuki Iguchi
Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto, Japan
Int J Syst Evol Microbiol 61:810-5. 2011..nov., sp. nov. is proposed. The type strain of the type species is HT12(T) (?=?NBRC 106162(T) ?=?DSM 23269(T) ?=?ATCC BAA-2070(T))... - Physiological role of S-formylglutathione hydrolase in C(1) metabolism of the methylotrophic yeast Candida boidiniiHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
Microbiology 149:1971-9. 2003..These results suggested that FGH is not essential but necessary for optimal growth on methanol. This is believed to be the first report of detailed analyses of the FGH1 gene in a methylotrophic yeast strain... - Gene structure and regulation of alkane monooxygenases in propane-utilizing Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7Tetsuya Kotani
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto, Japan
J Biosci Bioeng 102:184-92. 2006..TY-6 and that propane is oxidized to 1-propanol and 2-propanol through both terminal and subterminal oxidations in Pseudonocardia sp. TY-7. This study reveals the diversity of propane metabolism present in microorganisms... - Yap1-regulated glutathione redox system curtails accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastorisTaisuke Yano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto 606 8502, Japan
Eukaryot Cell 8:540-9. 2009..Thus, the PpYap1-regulated glutathione redox system has two important roles, i.e., HCHO metabolism and detoxification of reactive oxygen species... - The ribulose monophosphate pathway substitutes for the missing pentose phosphate pathway in the archaeon Thermococcus kodakaraensisIzumi Orita
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Kyoto 606-8502, Japan
J Bacteriol 188:4698-704. 2006.... - Anaerobic degradation of aromatic compounds by magnetospirillum strains: isolation and degradation genesYoshifumi Shinoda
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan
Biosci Biotechnol Biochem 69:1483-91. 2005..It was confirmed that the genus Magnetospirillum represents the third genus of denitrifying bacteria capable of degrading aromatic compounds under anaerobic conditions, besides the genera Thauera and Azoarcus... - The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathwayIzumi Orita
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo ku, Kyoto 606 8502, Japan
J Bacteriol 187:3636-42. 2005..These and other biochemical characterizations of the PH1938 gene product suggest that the ribulose monophosphate pathway plays a significant role in the archaeon under extreme environmental conditions... - The ribulose monophosphate pathway operon encoding formaldehyde fixation in a thermotolerant methylotroph, Bacillus brevis S1Hiroya Yurimoto
Division of Applied Life Sciences, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
FEMS Microbiol Lett 214:189-93. 2002..Sequence analysis also suggested that the hps and phi genes constitute an RuMP operon. The gene organization of the RuMP operon and its surrounding region are unique among bacteria possessing the RuMP pathway genes... - Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastorisTaisuke Yano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Biosci Biotechnol Biochem 73:1404-11. 2009..PpGpx1, but not the ScTsa1 or SpTpx1 homolog PpTsa1, functions as a H(2)O(2) sensor and activates PpYap1. This study is the first demonstration of a yeast Yap1 family protein activated during conventional metabolism... - Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in miceChika Nishizaki
Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Free Radic Biol Med 51:773-9. 2011..These results indicate that hCAT-R9 and hCAT-ABP, both of which have a long retention time in the peritoneal cavity, can be effective at inhibiting surgery-induced peritoneal metastasis... - Alcohol dehydrogenases that catalyse methyl formate synthesis participate in formaldehyde detoxification in the methylotrophic yeast Candida boidiniiHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan
Yeast 21:341-50. 2004.... - Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastorisKyoko Egawa
Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo ku, Kyoto 606 8501, Japan
Protein Expr Purif 64:47-54. 2009..Furthermore, fed-batch fermentation yielded 90 mg of rPMP22 protein from 4L of culture. This is the first report to demonstrate the overproduction of a recombinant rPMP22 in the membrane compartments of P. pastoris... - Assimilation of formaldehyde in transgenic plants due to the introduction of the bacterial ribulose monophosphate pathway genesLi Mei Chen
Graduate School of Biostudies, Kyoto University, Sakyo ku, Kyoto, Japan
Biosci Biotechnol Biochem 74:627-35. 2010..Our results provide a novel strategy for phytoremediation of HCHO pollution, and also represent the first step toward the production of plants that can assimilate natural gas-derived C(1) compounds... - Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thaliana leavesKosuke Kawaguchi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
PLoS ONE 6:e25257. 2011..Thus, the present study sheds light on the life cycle and physiology of yeast in the natural environment at both the molecular and cellular levels... - Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1Yoshifumi Shinoda
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto 606 8502, Japan
Appl Environ Microbiol 70:1385-92. 2004..On the basis of these results, it is concluded that strain DNT-1 modulates the expression of two different initial pathways of toluene degradation according to the availability of oxygen in the environment... - A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomesTaisuke Yano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo ku, Kyoto, Japan
Mol Cell Biol 30:3758-66. 2010.... - Propane monooxygenase and NAD+-dependent secondary alcohol dehydrogenase in propane metabolism by Gordonia sp. strain TY-5Tetsuya Kotani
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Sakyo-ku, Kyoto 606-8502, Japan
J Bacteriol 185:7120-8. 2003..On the basis of these results, we conclude that Gordonia sp. strain TY-5 oxidizes propane by monooxygenase-mediated subterminal oxidation via 2-propanol... - Purification and characterization of benzoate-CoA ligase from Magnetospirillum sp. strain TS-6 capable of aerobic and anaerobic degradation of aromatic compoundsKosuke Kawaguchi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
FEMS Microbiol Lett 257:208-13. 2006..These results indicate that a single benzoate-CoA ligase is expressed and benzoate is converted into benzoyl-CoA under both aerobic and anaerobic conditions in Magnetospirillum sp... - Soluble and particulate methane monooxygenase gene clusters of the type I methanotroph Methylovulum miyakonense HT12Hiroyuki Iguchi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
FEMS Microbiol Lett 312:71-6. 2010..However, unlike other methanotrophs, M. miyakonense HT12 harbored only a single copy of the pmoCAB gene. These data provide new insights into the structure of MMO genes... - Trm1p, a Zn(II)2Cys6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidiniiYu Sasano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Sakyo ku, Kyoto 606 8502, Japan
Eukaryot Cell 7:527-36. 2008..Thus, the TRM1 gene encodes a master transcriptional regulator responsible for methanol-specific gene activation in the methylotrophic yeasts... - Stimulation of methanotrophic growth in cocultures by cobalamin excreted by rhizobiaHiroyuki Iguchi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Appl Environ Microbiol 77:8509-15. 2011..Growth stimulation by cobalamin was also observed for three other gammaproteobacterial methanotrophs. These results suggest that microbial interactions through cobalamin play an important role in methane oxidation in various ecosystems... - Control of hypoxia-induced tumor cell adhesion by cytophilic human catalaseTomoya Yata
Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Science, Kyoto University, Sakyo ku, Kyoto 606 8501, Sakyo ku, Japan
Free Radic Biol Med 47:1772-8. 2009..hCAT-(RGD)3 or hCAT-R9 efficiently inhibited these changes compared with hCAT. These results indicate that cytophilic hCAT-(RGD)3 and hCAT-R9 are effective in inhibiting hypoxia-induced tumor cell adhesion to endothelial cells... - Lag-phase autophagy in the methylotrophic yeast Pichia pastorisShun Ichi Yamashita
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Genes Cells 14:861-70. 2009..Together, these data demonstrate the importance of amino acid recycling by autophagy during a cell-remodeling process... - Physiological role of the glutathione-dependent formaldehyde dehydrogenase in the methylotrophic yeast Candida boidiniiBumjun Lee
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan
Microbiology 148:2697-704. 2002..These results indicated that FLD, but not FDH, is essential for growth of C. boidinii on methanol... - Gene-tagging mutagenesis in the methylotrophic yeast Candida boidiniiYu Sasano
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake, Kyoto, Japan
J Biosci Bioeng 104:86-9. 2007..A large number of mutants were obtained, indicating that this method is a powerful tool for random mutagenesis in C. boidinii... - The pro-peptide of Streptomyces mobaraensis transglutaminase functions in cis and in trans to mediate efficient secretion of active enzyme from methylotrophic yeastsHiroya Yurimoto
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan
Biosci Biotechnol Biochem 68:2058-69. 2004..A recombinant Candida boidinii strain was found to secrete active TGase up to 1.83 U/ml (about 90 mg/l) after 119 h of cultivation... - Role of alpha-methylacyl coenzyme A racemase in the degradation of methyl-branched alkanes by Mycobacterium sp. strain P101Yasuyoshi Sakai
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan
J Bacteriol 186:7214-20. 2004..The reduced growth of mutant H1 on pristane suggests that propionyl-CoA is available for cell propagation through the 2-methyl citric acid cycle, since propionyl-CoA is produced through beta-oxidation of pristane... - Distribution of pink-pigmented facultative methylotrophs on leaves of vegetablesMasayuki Mizuno
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University
Biosci Biotechnol Biochem 76:578-80. 2012..fujisawaense DSM5686(T) and M. radiotolerans JCM2831(T) respectively, suggesting that there is specific interaction between perilla and the PPFMs... - Msn5p is involved in formaldehyde resistance but not in oxidative stress response in the methylotrophic yeast Candida boidiniiZhenyu Zhai
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan
Biosci Biotechnol Biochem 76:299-304. 2012..boidinii when the cells were treated with high concentrations of formaldehyde or ethanol, but was predominantly associated with the nuclei following treatment with hydrogen peroxide...