Takahiro Nakamura

..After the clinical application of this new surgical treatment, some patients experienced epithelial and subepithelial opacities. We applied our procedure again in these patients to achieve successful ocular surface reconstruction...

..Our in vivo and in vitro findings indicate that p75 is a potential marker of oral keratinocyte stem/progenitor cells and that some neurotrophin/p75 signaling affects cell growth and survival...

..Our data provide the first evidence that Hes1 regulates corneal development and the homeostatic function of corneal epithelial stem/progenitor cells...

..To evaluate the use of sterilized, freeze-dried amniotic membrane (FD-AM) transplantation for pterygium surgery...

..In the current study, the expression of clusterin was examined in conjunctivalized corneas with severe ocular surface disease...

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..To determine the feasibility of using human amniotic membrane (AM) as a substrate for culturing oral epithelial cells and to investigate the possibility of using autologous cultivated oral epithelial cells in ocular surface reconstruction...

..We succeeded in culturing primary corneal limbal epithelial cells taken from minimal biopsy and, once grown, transplanting them on denuded amniotic membrane (AM)...

..Bone marrow (BM)-derived stem cells are thought to possess extensive differentiation capacity. The present study was conducted to investigate the characteristics and distribution of these cells in the normal mouse cornea...

..To determine the epithelial lineage of origin of surgically removed grafts after autologous cultivated oral mucosal epithelial transplantation (COMET)...

..To determine outcomes of transplants of cultivated autologous oral epithelial cells in patients with severe ocular surface disorders...

..To investigate the possibility of using cultivated oral epithelial cells in ocular surface reconstruction...

..To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction...

..Thus, TT-FDAM retained most of the physical, biological, and morphological characteristics of native AM, consequently it is a useful biomaterial for ocular surface reconstruction...

..The purpose of this study was to evaluate sutureless keratoplasty using a chemically-defined bioadhesive (CDB) made from food or medical additives...

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..The use of AS is of clinical importance in the development of autologous xenobiotic-free bioengineered ocular surface equivalents for clinical transplantation...

..We evaluated the suitability of transplanted cultivated human conjunctival epithelium (HCjE) as a corneal epithelium replacement in rabbits with total corneal and limbal deficiency...

..To clarify the relationship between clinical symptoms and histological status in patients with ocular cicatricial pemphigoid (OCP) and Stevens-Johnson syndrome (SJS)...

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..To evaluate the efficacy and safety of a novel sutureless transplantation of bioadhesive-coated, sterilized, freeze-dried amniotic membrane (FD-AM) for ocular surface reconstruction...

..The TJ complex includes the integral transmembrane proteins occludin and the claudins, and some membrane-associated proteins such as ZO-1. In this paper, we investigated the barrier function and the expression of TJ related proteins...

..We suggest that this surgical modality may be both safe and useful, especially in younger patients with the most severe ocular surface disorders...

..To determine the long-term epithelial lineage of origin of surgically removed grafts after allogeneic cultivated corneal limbal epithelial transplantation (CLET)...

..The new cell-suspension technique was compared with the existing explant method...

..Ssp1 and Ppe1 thus seem to antagonize in utilizing limited glucose. We also show that Ppk9 and Ssp2 are the catalytic subunits of AMPK and AMPK-related kinases, respectively, which bind to common beta-(Amk2) and gamma-(Cbs2) subunits...

..Although long-term prognosis and cell biologic behavior of tissue-engineered epithelium after transplantation should be carefully monitored, these recent surgical treatments have opened a new field for ocular surface reconstruction...

..To assess the surgical combination of autologous cultivated oral mucosal epithelial transplantation and eyelid surgery used to treat patients with severe ocular surface disease and entropion...

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..We evaluated constitutive cellular trafficking into the cornea in the naive state and investigated how corneal inflammation may be initiated by various stimuli...

..The focus was factors with significantly higher gene expression in corneal epithelium (CE) than in conjunctival epithelium (CJE)...

..Flow cytometric study has shown that they comprise integrin beta1 high-expressing (beta1 2+) and low-expressing (beta1+) populations. This study was undertaken to determine their proliferation characteristics...

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..To achieve this, a matrix that can serve as a carrier for cHCECs is needed. The present study was conducted to examine the feasibility of using amniotic membrane (AM) as a carrier for this application...

..Securin bound to separase was hyperphosphorylated. The stresses could not rescue the indestructible Cut2 and Rad21 mutants. We postulate that the stresses produce the hyperchaperonic form of Cut2 that can rescue separase mutations...

..It has the ability to fix AM to the ocular surface for a long time-period without additional inflammation, scarring, or damage to the surrounding tissues...

..Participants This study involved 19 eyes of 17 patients who received autologous COMET for total limbal stem-cell deficiency...

..To reexamine whether the alpha5 chain of type IV (alpha5[IV]) collagen, thought to be absent, is in fact present in human amniotic membrane...

..To investigate the expression and cellular distribution of the tight junction-related proteins occludin, claudin and ZO-1 in human corneal epithelium...

..This article aims to describe the recent advances and current development of ocular surface reconstruction from both basic science and clinical aspects...

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..CONCLUSION: Various keratinization-related proteins and clusterin are most likely to be involved in the pathogenesis of cicatrizing ocular surface diseases...

..In comparison with CCE, COE showed decreased expression of anti-angiogenic factors particularly TSP-1. This different expression may relate to the superficial peripheral neovascularization encountered after COE transplantation...

..To characterize the long-term incorporation of transplanted amniotic membrane into corneal stroma...

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..Therefore, AM-cultured oral epithelial sheets might be a useful biomaterial for oral mucosal reconstruction...

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..On corneal sections, anti-keratin 5 produced staining similar to that of rabbit IgG. These findings suggest that rabbit IgG binds to keratin 5 in the human corneal epithelium via the Fc region of the molecule...

..It seems that 201Tl is not useful for the diagnosis. Nevertheless, the discordance between 201Tl uptake in primary tumors and skeletal lesions allows speculation on the mechanism of 201Tl accumulation in skeletons...

..Iodine-131 uptake was seen in the right upper abdominal quadrant of a patient with postoperative differentiated thyroid cancer that corresponded with a large liver cyst...

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..In this study, we examined the production of cytokines in hPDL cells stimulated with Porphyromonas gingivalis as compared with P. intermedia...

..Our graft thus shows promise for repair of biliary defects and should lead to development of a new treatment for biliary reconstruction...

..Periodic examinations with 123I-MIBG scintigraphy appears to be a useful technique for the detection of the recurrences...

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..The KHS will provide new evidence for the management of blood pressure in hypertensive patients with high risk...

..Of particular interest, one of these mutations (Tyr429Asp), detected in both affected individuals in one of our pedigrees, is the first mutation to be identified within the alpha-helix-termination motif in type I keratin...

..Fixation of the catheter tip with combined use of coils and NBCA--Lipiodol mixture to the gastroduodenal artery is important to prevent dislocation of the port-catheter system...

..Adiponectin is a collagen-like plasma protein specifically synthesized in adipose tissue. Plasma adiponectin concentrations are decreased in obesity whereas it is adipose-specific...

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..To report five unrelated Japanese individuals with "gelatino-lattice corneal dystrophy that clinically resembled, to some extent, gelatinous drop-like corneal dystrophy and lattice corneal dystrophy type 1...

..CONCLUSION: One cause of nontumorous vascular abnormalities adjacent to the falciform ligament as shown on angiographic helical CT is aberrant gastric venous inflow to this region...

..These results showed that bFGF-containing collagen gel is suitable not only for an acellular scaffold for tissue engineering but also for an effective tropic factor vehicle in vivo...

..The responses diminished after the excision of the regenerated portion of HGN. These results proved the regeneration of HGN and this nerve conduit will be great help for patients who suffer from urinary and seminal disturbances...

..08, p = 0.018, Bonferroni correction, p = 0.126). Our data suggested that mutations at the -45 locus in the promoter region of the CCK gene may influence vulnerability to hallucinations in PD patients treated with L-dopa...

..This newly developed nerve conduit has the potential to enhance peripheral nerve regeneration across longer gaps commonly encountered in clinical settings...

..A new tracheal prosthesis was therefore designed for reconstruction of the tracheal bifurcation and applied for experimental carinal replacement in dogs...

..Histone H2B thus plays an important role in centromere/kinetochore formation...

..The purpose of this study was to elucidate the possibility of the combination therapy of NK4, a competitive antagonist for HGF...

..The risk of brain infarction should be taken into consideration when a trans-left subclavian arterial access route is used for the percutaneous implantation of a port-catheter system for hepatic arterial infusion chemotherapy...

..Our nerve conduit can guide peripheral nerve elongation and lead to favorable functional recovery across a wider nerve gap than previously reported artificial nerve conduits...

..Here we report a case of endophthalmitis due to Pseudomonas aeruginosa that occurred after a perforating injury caused by a cat claw. The eye was treated by pars plana vitrectomy...

..In the present investigation, a collagen sponge that contained TGF-beta1 was placed at a mandibular defect and the osteogenic effects of collagen-TGF-beta1, complex were evaluated...

..To evaluate the outcome and adverse effects in patients with osteosarcoma treated with very high-dose definitive intraoperative radiotherapy (IORT), with the intention of saving the affected limb...

..These data suggest that adiponectin is a plasma protein produced by adipose tissue and accumulates in vascular walls when the endothelial barrier is injured...

..CONCLUSIONS: The acellular heparinized xenograft has sufficient mechanical properties and was successfully replaced with host tissue, and is thus a promising new type of vascular prosthesis...

..No mutation, nor polymorphism was found in the coding region of BRS-3, suggesting that mutation of this gene is not a major cause of obesity in humans...

..In the present study, we investigated whether blood typing is necessary in immunosuppressant-free tracheal allotransplantation in dogs...

..Consequently, pseudolesions around the gallbladder usually can be distinguished from tumorous lesions by adding prostaglandin E(1) injection via the superior mesenteric artery during CT hepatic arteriography...

..The formation of a pseudoaneurysm of the deep circumflex iliac artery after the harvesting of an anterior iliac bone graft for spinal fusion is reported...

..To develop a method for eliminating the epithelium and mixed glands from tracheal grafts by detergent treatment and evaluate these grafts for immunosuppressant-free allotransplantation in dogs...

..To examine the outcome of a triamcinolone acetonide (TA) assisted pars plana vitrectomy (PPV) for refractory uveitis...

..Enhanced cardiac sympathetic function in these patients may contribute to the leptin levels before nCPAP treatment and vice versa...

..This population-based epidemiological study was conducted in Miyama, a village located in a mountain area in Wakayama Prefecture, Japan...

..To investigate intraoperative visibility and long-term clinical outcome following triamcinolone acetonide (TA)-assisted pars plana vitrectomy (PPV) for proliferative vitreoretinopathy (PVR)...

..CONCLUSIONS. Various keratinization-related proteins, transglutaminase 1 included, are most likely involved in the pathogenesis of cicatrizing ocular surface diseases...

..30-33.78). These findings suggest that a long duration of multiple risks is involved in the onset of IHD in Japanese employees, and that annual ECG monitoring as part of the medical examination was important in the prognosis...

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..Moreover, expression of human A-C1 mRNA was detected at a high level in human osteosarcoma-derived U2OS cells in vitro. By FISH analysis the human A-C1 gene (HRASLS) was mapped to human chromosome 3q28--> q29...

..The BA cement bonded to the bone through a Ca-P-rich reactive layer, which was twice as thick after 7 years than it was at 26 weeks. No adverse effects of BA cement were observed during the 7-year observation period...

..FKBP23 mRNA is expressed most strongly in heart, lung, and testis, beginning at day 8.5 of embryonic development. The FKBP23 gene was mapped to mouse chromosome 2...

..To characterize the clinicopathologic features of ataxic and painful forms of paraneoplastic neuropathy...

..Our data support the hypothesis that Ihh stimulated the late-phase chondrogenic differentiation in differentiated ATDC5 cells and upregulated the gene expression of OPGL in these cells...

..Acoustic properties differed from mechanical stiffness properties, which were determined using indentation. Ultrasound may detect properties of the surface collagen of the articular cartilage...

..These results suggest that galectin-12, an adipose-expressed galectin-like molecule, may participate in the apoptosis of adipocytes...

..Fission yeast Bir1/Pbh1 is essential for normal mitosis...

..We report a case of small pulmonary arteriovenous fistulae precisely diagnosed by a combination of selective pulmonary arteriography and scintigraphy during (99)Tc(m)-macroaggregated albumin injection via the peripheral pulmonary artery...

..The present study investigated whether adiponectin could modulate endothelial function and relate to coronary disease...

..Also, this program will be useful for detecting LV diastolic dysfunction in various cardiac diseases before LV systolic dysfunction becomes evident...

..The prostate-type hippostasin, which has 32 extra amino acids at the N-terminal end, is expressed only in the prostate...