Research Topics
Species | Mikiko C SiomiSummaryAffiliation: Keio University Country: Japan Publications
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Detail Information
Publications
How selfish retrotransposons are silenced in Drosophila germline and somatic cellsMikiko C Siomi
Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo, Japan
FEBS Lett 582:2473-8. 2008..In this review, we address RNA silencing mechanisms that silence retrotransposons, a subset of TEs, and discuss how germline and somatic cells are equipped with different retrotransposon silencing mechanisms...
PIWI-interacting small RNAs: the vanguard of genome defenceMikiko C Siomi
Keio University School of Medicine, Tokyo 160 8582, Japan
Nat Rev Mol Cell Biol 12:246-58. 2011..Now, much progress is being made into our understanding of their biogenesis and molecular functions, including the specific subcellular compartmentalization of the piRNA pathway in granular cytoplasmic bodies...
Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in DrosophilaKuniaki Saito
Keio University School of Medicine, Tokyo, Japan
Genes Dev 24:2493-8. 2010..Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing...
How does the royal family of Tudor rule the PIWI-interacting RNA pathway?Mikiko C Siomi
Keio University School of Medicine, Tokyo 160 8582, Japan
Genes Dev 24:636-46. 2010..Although Tud domains in Tud family members are known to be sDMA-binding modules, involvement of the Tudor family at the molecular level in the piRNA pathway has only recently come into focus...
piRNA-mediated silencing in Drosophila germlinesMikiko C Siomi
Keio University School of Medicine, Tokyo 160 8582, Japan
Semin Cell Dev Biol 21:754-9. 2010....
RNA silencing in germlines--exquisite collaboration of Argonaute proteins with small RNAs for germline survivalMikiko C Siomi
Keio University School of Medicine, Tokyo 160 8582, Japan
Curr Opin Cell Biol 21:426-34. 2009..This chapter summarizes the exquisite collaboration of Argonaute proteins with small RNAs in the RNA silencing mechanisms necessary for germline survival in Drosophila and mice...
A regulatory circuit for piwi by the large Maf gene traffic jam in DrosophilaKuniaki Saito
Keio University School of Medicine, Tokyo 160 8582, Japan
Nature 461:1296-9. 2009..Thus, in gonadal somatic cells, tj gives rise simultaneously to two different molecules: the TJ protein, which activates Piwi expression, and piRNAs, which define the Piwi targets for silencing...
Chapter 16. How to define targets for small guide RNAs in RNA silencing: a biochemical approachMikiko C Siomi
Keio University School of Medicine, Tokyo, Japan JST, CREST, Saitama, Japan
Methods Enzymol 449:345-55. 2008..The method discussed is useful for defining targets for the small RNAs that function in RNA silencing...
Molecular mechanisms that funnel RNA precursors into endogenous small-interfering RNA and microRNA biogenesis pathways in DrosophilaKeita Miyoshi
Keio University School of Medicine, Tokyo 160 8582, Japan
RNA 16:506-15. 2010..These results suggest that the precursor RNA structure determines the biogenesis mechanism of esiRNAs and miRNAs, thereby implicating hairpin structures with long stems as intermediates in the evolution of Drosophila miRNA...
Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testisAkihiro Nagao
Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
RNA 16:2503-15. 2010..These findings suggest that the impacts of armi mutants on the operation of the piRNA pathway are variable in germ cells of fly testes...
Functional involvement of Tudor and dPRMT5 in the piRNA processing pathway in Drosophila germlinesKazumichi M Nishida
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
EMBO J 28:3820-31. 2009..Thus, in germline cells, piRNAs are quality-controlled by dPRMT5 that modifies PIWI proteins, in tight association with Tud...
A direct role for Hsp90 in pre-RISC formation in DrosophilaTomohiro Miyoshi
Keio University School of Medicine, Tokyo, Japan
Nat Struct Mol Biol 17:1024-6. 2010....
Small RNA-mediated quiescence of transposable elements in animalsKuniaki Saito
Department of Molecular Biology, Keio University School of Medicine, Tokyo 160 8582, Japan
Dev Cell 19:687-97. 2010....
Biochemical analyzes of endogenous argonaute complexes immunopurified with anti-Argonaute monoclonal antibodiesKeita Miyoshi
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
Methods Mol Biol 725:29-43. 2011..Furthermore, we will also explain how various parameters, such as antibody properties and buffer conditions, can alter the production and interpretation of experimental data...
Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOCKaoru Sato
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan
Genes Dev 25:2361-73. 2011..These findings suggest a model in which Mael serves as a platform that nucleates other MTOC components to form a functional MTOC in early oocyte development, which is independent of Chk2 activation and DNA damage signaling...
Posttranscriptional regulation of microRNA biogenesis in animalsHaruhiko Siomi
Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
Mol Cell 38:323-32. 2010..Here, we discuss recent studies that shed light on how multiple steps in the miRNA biogenesis pathway are regulated to modulate miRNA function in animals...
On the road to reading the RNA-interference codeHaruhiko Siomi
Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
Nature 457:396-404. 2009....
Characterization of the miRNA-RISC loading complex and miRNA-RISC formed in the Drosophila miRNA pathwayKeita Miyoshi
School of Medicine, Keio University, Tokyo 160 8582, Japan
RNA 15:1282-91. 2009..The AGO1-PIWI mutant showed no Slicer activity but associates with mature miRNA. These results indicate that these domains are required differently for miRLC and miRISC formation in the miRNA pathway...
Stress signaling etches heritable marks on chromatinHaruhiko Siomi
Department of Molecular Biology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku ku, Tokyo 160 8582, Japan
Cell 145:1005-7. 2011..Seong et al. (2011) now reveal how stress-induced signaling through dATF-2 disrupts heterochromatin and leaves heritable marks that influence patterns of gene expression in subsequent generations...
