Research Topics
Genomes and Genes | Toshihisa OhtsukaSummaryAffiliation: KAN Research Institute Country: Japan Publications
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Detail Information
Publications
Gas6, a new regulator of chondrogenic differentiation from mesenchymal cellsHiraku Motomura
Department of Orthopaedic Surgery, Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
Biochem Biophys Res Commun 357:997-1003. 2007..Gas6 signaling activated the phosphorylation of ERK1/2, SAPK/JNK, and Akt, but not p38 MAPK. These results suggest that Gas6 negatively regulates chondrogenic differentiation, at least through the MAPK pathway...- Cast: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and munc13-1Toshihisa Ohtsuka
KAN Research Institute Inc, Kyoto 600 8815, Japan
J Cell Biol 158:577-90. 2002..These results suggest that these CAZ proteins are at least partly transported on the same vesicles during synapse formation... - Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter releaseEtsuko Takao-Rikitsu
KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
J Cell Biol 164:301-11. 2004..These results indicate that CAST serves as a key component of the CAZ structure and is involved in neurotransmitter release by binding these CAZ proteins... - SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter releaseEiji Inoue
KAN Research Institute, Kyoto 600 8815, Japan
Neuron 50:261-75. 2006..Furthermore, SAD-B phosphorylates the active zone protein RIM1 but not Munc13-1. These results suggest that mammalian SAD kinase presynaptically regulates neurotransmitter release... - ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cellsEiji Inoue
KAN Research Institute, Kyoto 600 8815, Japan
Genes Cells 11:659-72. 2006..These results suggest that ELKS is involved in Ca(2+)-dependent exocytosis from PC12 cells at least partly via the RIM2-Munc13-1 pathway... - CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CASTMaki Deguchi-Tawarada
KAN Research Institute, Kyoto Research Park, Shimogyo ku, Kyoto 600 8815, Japan
Genes Cells 9:15-23. 2004..Accordingly, the original CAST is tentatively re-named CAST1. These results indicate that CAST2 is a new component of the CAZ and, together with CAST1, may be involved in the formation of the CAZ structure... - Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processesShigeki Kakunaga
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Osaka, Japan
J Cell Sci 118:1267-77. 2005.... - Three-dimensional architecture of presynaptic terminal cytomatrixLea Siksou
INSERM U789, Ecole Normale Superieure, 75005 Paris, France
J Neurosci 27:6868-77. 2007..This 3D analysis reveals the morphological constraints exerted by the presynaptic molecular scaffold. SVs are tightly interconnected in the axonal bouton, and this network is preferentially connected to the AZ... - The active zone protein CAST directly associates with Ligand-of-Numb protein XSusumu Higa
Department of Clinical and Molecular Pathology, Graduate School of Medicine Faculty of Medicine, University of Toyama, Toyama 930 0194, Japan
Biochem Biophys Res Commun 354:686-92. 2007..Moreover, exogenously expressed LNX was partially colocalized with endogenous CAST in the axonal varicosities of cultured rat hippocampal neurons. These results suggest that CAST and LNX might form a protein complex in neurons... - [Structure and function of the presynaptic active zones]Toshihisa Ohtsuka
Department of Clinical and Molecular Pathology, Faculty of Medicine/Graduate School of Medicine, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan
Seikagaku 78:979-86. 2006 - SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle releaseIkuko Yao
Mitsubishi Kagaku Institute of Life Sciences MITILS, 11 Minamiooya, Machida, Tokyo 194 8511, Japan
Cell 130:943-57. 2007..Thus, SCRAPPER has an essential function in regulating proteasome-mediated degradation of RIM1 required for synaptic tuning... - ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence miMica Ohara-Imaizumi
Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
Mol Biol Cell 16:3289-300. 2005..Moreover, attenuation of ELKS expression by small interfering RNA reduced the glucose-evoked insulin release. These data suggest that the CAZ-related protein ELKS functions in insulin exocytosis from pancreatic beta cells... - Rab6 regulates transport and targeting of exocytotic carriersIlya Grigoriev
Department of Cell Biology, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands
Dev Cell 13:305-14. 2007..Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space... - Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue of exocytotic defects in Munc13-1 deficient pancreatic beta-cellsEdwin P Kwan
Department of Physiology, University of Toronto, Toronto, Ontario, Canada
Diabetes 56:2579-88. 2007..We had reported that haplodeficient Munc13-1(+/-) mouse islet beta-cells exhibited reduced insulin secretion, causing glucose intolerance. Munc13-1 binds Epac2 and Rim2, but their functional interactions remain unclear... - Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctionsTakashi Tokoro
Department of Clinical and Molecular Pathology, Graduate School of Medicine Faculty of Medicine, University of Toyama, Sugitani, Toyama, Japan
Neuroreport 18:313-6. 2007..These results suggest that at least ELKS and Piccolo, but not CAST, are components of neuromuscular junction synapses in the peripheral nervous system... - Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labelingAkari Hagiwara
Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki 444 8787, Japan
J Comp Neurol 489:195-216. 2005..These results support the involvement of the t-SNARE proteins in exocytotic fusion in the AZ and the role of CAST in specialization of the membrane domain for the AZ... - Active zone protein CAST is a component of conventional and ribbon synapses in mouse retinaMaki Deguchi-Tawarada
KAN Research Institute, Kyoto 600-8815, Japan
J Comp Neurol 495:480-96. 2006..Both proteins also localized in the vicinity of the presynaptic plasma membrane of conventional synapses in the retina. These results indicated that CAST and ELKS were novel components of the presynaptic apparatus of mouse retina... - CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5betaGideon Lansbergen
MGC Department of Cell Biology and Genetics, Erasmus Medical Center, 3000 DR Rotterdam, The Netherlands
Dev Cell 11:21-32. 2006..We propose that LL5beta and ELKS can form a PIP3-regulated cortical platform to which CLASPs attach distal microtubule ends... - Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampusMaiko Inagaki
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine Faculty of Medicine, Suita 565 0871, Japan
J Comp Neurol 460:514-24. 2003..These results indicate that ZO-1 colocalizes with nectin and afadin at the PAs and that the nectin-afadin system is involved in the localization of ZO-1...