Research Topics
| Lorenzo DragoSummaryAffiliation: University of Milan Country: Italy Publications
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Publications
Comparative bactericidal activity of fluoroquinolones against clinical isolates resistant to fluoroquinolonesL Drago
Laboratory of Clinical Microbiology, Dept of Clinical Sciences, L Sacco Teaching Hospital, University of Milan, Italy
J Chemother 15:118-23. 2003..pneumoniae by levofloxacin after 12 hours. In conclusion, the maximum levofloxacin concentrations achievable at certain body sites allowed killing even of strains defined as resistant by conventional breakpoints...
Microbiological evaluation of commercial probiotic products available in ItalyL Drago
Laboratory of Clinical Microbiology, Dept of Clinical Sciences L Sacco, L Sacco Teaching Hospital, University of Milan, Italy
J Chemother 16:463-7. 2004..Our results suggest the need for clear legislation and adequate control of the manufacturing of probiotic products...
A comparative in-vitro evaluation of resistance selection after exposure to teicoplanin, vancomycin, linezolid and quinupristin-dalfopristin in Staphylococcus aureus and Enterococcus sppL Drago
Laboratory of Clinical Microbiology, Department of Preclinical Science, LITA Vialba, University of Milan, Milan, Italy
Clin Microbiol Infect 14:608-11. 2008....
Modulation of biofilm of strains isolated from patients with chronic obstructive pulmonary disease by levofloxacin, moxifloxacin, ciprofloxacin, amoxicillin/clavulanic acid and ceftriaxoneL Drago
Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Institute, Milan, Italy
Int J Immunopathol Pharmacol 24:1027-35. 2011..Levofloxacin may contribute to clear the reservoir of pathogens involved in chronic obstructive pulmonary disease, thus leading to decreased occurrence of acute exacerbations...
Effects of Lactobacillus salivarius LS01 (DSM 22775) treatment on adult atopic dermatitis: a randomized placebo-controlled studyL Drago
Department of Clinical Sciences Luigi Sacco, University of Milan, Milan, Italy
Int J Immunopathol Pharmacol 24:1037-48. 2011..This probiotic strain could have an important role in modulating Th1/Th2 cytokine profiles and could be considered as an important adjunctive therapy in the treatment of adult AD...
Two-stage revision surgery with preformed spacers and cementless implants for septic hip arthritis: a prospective, non-randomized cohort studyCarlo L Romano
Dipartimento di Chirurgia Ricostruttiva e delle Infezioni Osteo articolari Istituto Ortopedico I, R, C, C, S, Galeazzi, Milano, Italy
BMC Infect Dis 11:129. 2011....
Detection of respiratory viruses and atypical bacteria in children's tonsils and adenoidsLorenzo Drago
Laboratory of Microbiology, Department of Preclinical Sciences, LITA Vialba, Via GB Grassi 74, University of Milan, Milan 20157, Italy
J Clin Microbiol 46:369-70. 2008..Viruses were detected in 47.7% of the children and 37.3% of the specimens, with adenovirus and parainfluenza viruses being the most frequently detected microorganisms...
In vitro evaluation of antibiotics' combinations for empirical therapy of suspected methicillin resistant Staphylococcus aureus severe respiratory infectionsLorenzo Drago
Laboratory of Clinical Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Via GB Grassi 74, 20157 Milan, Italy
BMC Infect Dis 7:111. 2007..Combinations of antibiotics used in the empiric treatment of infections with suspected methicillin resistant Staphylococcus aureus etiology were investigated...
Effect of moxifloxacin on bacterial pathogenicity factors in comparison with amoxicillin, clarithromycin and ceftriaxoneL Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences, L Sacco Teaching Hospital, University of Milan, Italy
J Chemother 16:30-7. 2004..In conclusion, subinhibitory concentrations of moxifloxacin remarkably interfere with some bacterial pathogenic factors, thereby contributing to its antimicrobial activity...
Evaluation of antibacterial in vitro activity of moxifloxacin and its effects on pulmonary clearance of Klebsiella pneumoniae in an animal experimental modelLorenzo Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences L Sacco, University of Milan, Milan, Italy
Arzneimittelforschung 55:473-7. 2005..In conclusion, this study provides in vivo evidence of the antimicrobial properties of moxifloxacin against K. pneumoniae...
Comparison of nested PCR and real time PCR of Herpesvirus infections of central nervous system in HIV patientsLorenzo Drago
Laboratory of Clinical Microbiology, L Sacco Teaching Hospital and Department of Clinical Sciences L, Sacco, University of Milan, Via GB Grassi74, 20157 Milan, Italy
BMC Infect Dis 4:55. 2004..In this study nested PCR and real time PCR techniques for detection of Herpes simplex virus type 1 (HSV-1), Cytomegalovirus (CMV) and Epstein-Barr virus (EBV) in cerebrospinal fluid of HIV patients were compared...
In vitro synergy and selection of resistance by fluoroquinolones plus amikacin or beta-lactams against extended-spectrum beta-lactamase-producing Escherichia coliL Drago
Lab Clinical Microbiology, Dept Clinical Science L Sacco, Via GB Grassi 74, 20157 Milano, Italy
J Chemother 17:46-53. 2005..In conclusion, the combination of a fluoroquinolone with a beta-lactam or amikacin may provide improved antimicrobial activity and help limit the occurrence of resistance in ESBL-producing E. coli strains...
In vitro selection of resistance to clarithromycin in Streptococcus pneumoniae clinical isolatesL Drago
Laboratory of Clinical Microbiology, Dept of Clinical Sciences, L Sacco Teaching Hospital, University of Milan
J Chemother 17:161-8. 2005..Frequencies of mutation were lower for clarithromycin and moxifloxacin than for the comparators. At lung concentrations clarithromycin had limited potential to select for resistance...
In vitro selection of resistance in Pseudomonas aeruginosa and Acinetobacter spp. by levofloxacin and ciprofloxacin alone and in combination with beta-lactams and amikacinLorenzo Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences, L Sacco Teaching Hospital, University of Milan, Via GB Grassi 74, 20157 Milan, Italy
J Antimicrob Chemother 56:353-9. 2005....
Activity of a Lactobacillus acidophilus-based douche for the treatment of bacterial vaginosisLorenzo Drago
Laboratory of Clinical Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Milan, Italy
J Altern Complement Med 13:435-8. 2007....
Kinetic bactericidal activity of telithromycin, azithromycin and clarithromycin against respiratory pathogensL Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences L Sacco, University of Milan, Italy
APMIS 113:655-63. 2005..In conclusion, telithromycin proved to possess bactericidal activity against a wide range of respiratory pathogens, including strains resistant to common macrolides...
In vitro selection of antibiotic resistance in the probiotic strain Lactobacillus rhamnosus GG ATCC 53103L Drago
Laboratory of Microbiology, Department of Clinical Sciences Luigi Sacco, University of Milan, Milan, Italy
J Chemother 23:211-5. 2011....
Rapid, progressive neuropathic arthropathy of the hip in a patient co-infected with human immunodeficiency virus, hepatitis C virus and tertiary syphilis: case reportLorenzo Drago
Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy
BMC Infect Dis 11:159. 2011..This article reports the case of a man with tertiary syphilis presenting with rapidly evolving neuropathic arthropathy of the hip and extensive bone destruction...
Comparative evaluation of synergy of combinations of beta-lactams with fluoroquinolones or a macrolide in Streptococcus pneumoniaeL Drago
Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi Orthopaedic Institute, Milan, Italy
J Antimicrob Chemother 66:845-9. 2011..e. a macrolide with a protected penicillin or cephalosporin) against 100 isolates with different susceptibilities to macrolides and/or penicillin...
Microbiological evaluation of commercial probiotic products available in the USA in 2009L Drago
Laboratory of Microbiology, Department of Clinical Sciences Luigi Sacco, University of Milan, Milan, Italy
J Chemother 22:373-7. 2010..Our results suggest the need for adequate control of probiotic production as well as periodical screenings by competent organizations to monitor the effect of storage on product quality...
Macrolide resistance and in vitro selection of resistance to antibiotics in Lactobacillus isolatesLorenzo Drago
Lab of Clinical Chemistry and Microbiology, IRCCS Galeazzi Institute, Via R Galeazzi 4, 20161 Milan, Italy
J Microbiol 49:651-6. 2011..Since lactobacilli are often used as probiotics, their ability to acquire resistance should be evaluated for isolates candidate to be included in probiotics based products...
In vitro selection of resistance in Escherichia coli and Klebsiella spp. at in vivo fluoroquinolone concentrationsLorenzo Drago
Laboratory of Microbiology, Dept Preclinical Sciences LITA Vialba, University of Milan, Via GB Grassi 74, Milan 20157, Italy
BMC Microbiol 10:119. 2010..E. coli resistant strains selected after multi-step selection were characterized for DNA mutations by sequencing gyrA, gyrB, parC and parE genes...
Strain-dependent release of cytokines modulated by Lactobacillus salivarius human isolates in an in vitro modelLorenzo Drago
Laboratory of Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Milan, Italy
BMC Res Notes 3:44. 2010..Four strains of Lactobacillus salivarius, LDR0723, BNL1059, RGS1746 and CRL1528, were evaluated for their ability to modulate release of pro- and anti-inflammatory cytokines...
Should Lactobacillus sporogenes and Bacillus coagulans have a future?L Drago
Laboratory of Clinical Che, istry, IRCCS Galeazzi Hospital, University of Milan, Milan, Italy
J Chemother 21:371-7. 2009..Although the use of L. sporogenes spores as a probiotic has increased in recent years, clinical evidence of its benefits are limited to only a few studies involving small patient populations...
The safety of cefepime in the treatment of infectionLorenzo Drago
University of Milan, Laboratory of Clinical Microbiology, Department of Preclinical Science, LITA Vialba, Via GB Grassi 74, 20157 Milan, Italy
Expert Opin Drug Saf 7:377-87. 2008....
Selection of resistance of telithromycin against Haemophilus influenzae, Moraxella catarrhalis and streptococci in comparison with macrolidesLorenzo Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences L Sacco Teaching Hospital, University of Milan, Via GB Grassi 74, 20157 Milan, Italy
J Antimicrob Chemother 54:542-5. 2004..The in vitro abilities of telithromycin, azithromycin and clarithromycin to select for resistance were compared by testing isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and beta-haemolytic streptococci...
Activity of levofloxacin and ciprofloxacin in combination with cefepime, ceftazidime, imipenem, piperacillin-tazobactam and amikacin against different Pseudomonas aeruginosa phenotypes and Acinetobacter sppLorenzo Drago
Laboratory of Clinical Microbiology, Department of Clinical Sciences, L Sacco Teaching Hospital, University of Milan, Via G B Grassi 74, IT 20157 Milan, Italy
Chemotherapy 50:202-10. 2004..Combination therapy is used to widen the antimicrobial spectrum, minimize toxicity and prevent the emergence of resistant mutants...
In vitro activity of telithromycin against Haemophilus influenzae at epithelial lining fluid concentrationsElena De Vecchi
Laboratory of Clinical Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Via GB Grassi 74, 20159 Milan, Italy
BMC Microbiol 8:23. 2008..The secondary aim was to determine the influence of CO2 enriched atmosphere on bacterial susceptibility...
Differences in nasopharyngeal bacterial flora in children with nonsevere recurrent acute otitis media and chronic otitis media with effusion: implications for managementPaola Marchisio
Department of Pediatrics, University of Milan, Italy
Pediatr Infect Dis J 22:262-8. 2003..The results of nasopharyngeal cultures should be taken into account to avoid treatment with drugs that are ineffective and likely to select resistant organisms...
Diagnostic accuracy of nasopharyngeal swabs in detecting biofilm-producing bacteria in chronic adenoiditis: a preliminary studySara Torretta
Department of Specialist Surgical Sciences, Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, Universita degli Studi di Milano, Milan, Italy
Otolaryngol Head Neck Surg 144:784-8. 2011..To our knowledge, the accuracy of nasopharyngeal swabs in diagnosing BPB has never been assessed...
Bactericidal activity of levofloxacin, gatifloxacin, penicillin, meropenem and rokitamycin against Bacillus anthracis clinical isolatesLorenzo Drago
Laboratory of Clinical Microbiology, L Sacco Teaching Hospital, University of Milan, Via GB Grassi 74, 20157 Milan, Italy
J Antimicrob Chemother 50:1059-63. 2002....
In vitro antimicrobial activity of a novel propolis formulation (Actichelated propolis)L Drago
Dept of Preclinical Science LITA Vialba, University of Milan, Milano, Italy
J Appl Microbiol 103:1914-21. 2007..This study compared in vitro activities of Actichelated propolis (a multicomposite material obtained with mechano-chemichal activation) and of a hydroalcoholic extract of propolis...
Effects of three different fish oil formulations on Helicobacter pylori growth and viability: in vitro studyL Drago
L Sacco Teaching Hospital, University of Milan, Milano, Italy
J Chemother 11:207-10. 1999..Further in vitro and in vivo studies are needed before proposing fish oils as treatment of H. pylori infection...
Antimicrobial activity of thiamphenicol-glycinate-acetylcysteinate and other drugs against Chlamydia pneumoniaeA Lombardi
Laboratory of Clinical Microbiology, L. Sacco Teaching Hospital, University of Milan, Milan, Italy
Arzneimittelforschung 51:264-7. 2001..pneumoniae. These findings need further evaluation by clinical studies...
Antimycotic activity and phagocytosis effects of econazole in combination with ibuprofen isobuthanolammonium against vaginal strainsL Drago
Laboratory of Clinical Microbiology, Department of Preclinical Science, LITA Vialba, Milan, Italy
J Chemother 12:509-15. 2000..We conclude that the addition of ibuprofen isobuthanolammonium to econazole provides better in vitro antifungal activity. However, further studies are needed to elucidate the in vivo action of this formulation...
In vitro antimicrobial activity of propolis dry extractL Drago
Clinical Microbiology, L Sacco Teaching Hospital, University of Milan, Italy
J Chemother 12:390-5. 2000..pneumoniae, H. influenzae and M. catarrhalis, but not against Enterobacteriaceae. Time-kill curves demonstrated bacteriostatic rather than bactericidal activity of propolis, the latter being evident only at high concentrations...
Comparative effect of thiamphenicol glycinate, thiamphenicol glycinate N-acetylcysteinate, amoxicillin plus clavulanic acid, ceftriaxone and clarithromycin on pulmonary clearance of Haemophilus influenzae in an animal modelL Drago
Laboratory of Clinical Microbiology, Department of Preclinical Science, L I T A Vialba, Milan, Italy
Chemotherapy 46:275-81. 2000..Thiamphenicol glycinate (TG) and its derivative thiamphenicol glycinate N-acetylcysteinate (TGA) could be a valid therapeutic option in the treatment of respiratory tract infections...
Candida albicans cellular internalization: a new pathogenic factor?L Drago
Laboratory of Clinical Microbiology, Department of Preclinical Science and L Sacco Teaching Hospital, University of Milan, L I T A Vialba, Via G B Grassi 74, 20157 Milan, Italy
Int J Antimicrob Agents 16:545-7. 2000..This phenomenon could represent an interesting experimental model to testing drug interference in the development of therapeutic strategies against C. albicans infections...
Escherichia coli: effect of fosfomycin trometamol on some urovirulence factorsM R Gismondo
Institute of Medical Microbiology, Milan, Italy
J Chemother 6:167-72. 1994..coli were grown in the presence of sub-MIC concentrations of F.T. These results suggest that F.T. may be of clinical use as treatment for acute urinary tract infection and in pyelonephritis prophylaxis...
Serum and lung levels of thiamphenicol after administration of its glycinate N-acetylcysteinate ester in experimentally infected guinea pigsL Drago
Department of Preclinical Science, L I T A Vialba, L Sacco Teaching Hospital, University of Milan, Italy
Int J Antimicrob Agents 13:301-3. 2000..TAP serum levels were elevated at 1 h and remained detectable for 24 h after drug administration. Tissue lung levels were comparable to peak serum concentrations but remained higher and decreased more slowly than serum concentrations...
Review of probiotics available to modify gastrointestinal floraM R Gismondo
Clinical Microbiology Laboratory, L Sacco Teaching Hospital, University of Milan, Italy
Int J Antimicrob Agents 12:287-92. 1999....
In vitro selection of resistance in Streptococcus pneumoniae at in vivo fluoroquinolone concentrationsE De Vecchi
Laboratory of Microbiology, Department of Preclinical Sciences LITA Vialba, University of Milan, Milan, Italy
J Antimicrob Chemother 63:721-7. 2009..To compare the ability to select for resistance in Streptococcus pneumoniae of levofloxacin, moxifloxacin, ciprofloxacin and prulifloxacin...
Comparative in vitro activity of thiamphenicol-glycinate and thiamphenicol-glycinate-acetylcysteinate and other antimicrobials against respiratory pathogensL Drago
Laboratory of Clinical Microbiology, L Sacco Teaching Hospital, University of Milan, Milan, Italy
Arzneimittelforschung 51:315-24. 2001..aureus, respectively. TG and TGA are characterized by a wide spectrum of activity, comparable to that of recent commercialized antibiotics for treatment of respiratory tract infections...
Activity of levofloxacin and ciprofloxacin against urinary pathogensL Drago
Laboratory of Clinical Microbiology, Department of Preclinical Science, LITA Vialba, University of Milan, Via G B Grassi 74, 20157 Milano, Italy
J Antimicrob Chemother 48:37-45. 2001..125 x MIC. These findings suggest that levofloxacin is an effective alternative to ciprofloxacin in the treatment of urinary tract infections and that sub-inhibitory concentrations may contribute to efficacy...
In vitro screening of probiotic characteristics of some italian productsE De Vecchi
Laboratory of Microbiology and Clinical Microbiology, Dept of Preclinical Science LITA Vialba, University of Milan, Italy
J Chemother 20:341-7. 2008..longum, were able to adhere to Caco-2 cells, while S. boulardii and B. clausii showed reduced adherence to human intestinal cells. In conclusion, the products did not completely fulfill all probiotic attributes...
In vitro induction of resistance by tissue concentrations of azithromycin, clarithromycin, cefixime and amoxicillin/clavulanate in clinical isolates of Streptococcus pyogenesE De Vecchi
Laboratory of Microbiology and Clinical Microbiology, Dept of Preclinical Science LITA Vialba, University of Milan, Italy
J Chemother 18:379-88. 2006....
Antimicrobial activity and interference of tobramycin and chloramphenicol on bacterial adhesion to intraocular lensesL Drago
Microbiology Laboratory, Department of Clinical Sciences, L Sacco Teaching Hospital, University of Milan, Milan, Italy
Drugs Exp Clin Res 29:25-35. 2003..The data obtained in this study suggest a specific use of chloramphenicol in topical prophylaxis aimed at avoiding bacterial contaminations. However, further specific in vivo studies are needed to confirm these data...
Lack of in vitro and in vivo selection of bacterial resistance by roxithromycinL Drago
Laboratory of Clinical Microbiology, LITA, L Sacco Teaching Hospital, Milan, Italy
Chemotherapy 46:160-5. 2000....
Outcome of tonsillectomy in selected patients with PFAPA syndromeLorenzo Pignataro
Department of Otorhinolaryngological and Ophthalmological Sciences, University of Milan, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli and Regina Elena, Via F Sforza 35, Milan, Italy
Arch Otolaryngol Head Neck Surg 135:548-53. 2009..To assess the practicability of integrated medical and surgical management and the effectiveness of tonsillectomy in children with PFAPA syndrome (periodic fever, aphthous stomatitis, pharyngitis, and cervical lymphadenopathy)...
Healing of surgical site after total hip and knee replacements show similar telethermographic patternsCarlo Luca Romano
Dipartimento di Chirurgia Ricostruttiva e delle Infezioni Osteo articolari, Istituto Ortopedico I R C C S Galeazzi, Milan, Italy
J Orthop Traumatol 12:81-6. 2011....
Impact of cefetamet-pivoxil on intestinal microflora: in vivo studyM R Gismondo
Institute of Medical Microbiology, University of Milan, Italy
J Chemother 6:246-50. 1994..3 mg/Kg/die (standard dose) and a 28.6 mg/kg/die (daily dose) of cefetamet-pivoxil by oral route. The results obtained in vivo proved that cefetamet-pivoxil at therapeutic doses does not influence the normal intestinal microflora...
[Epidemiology and mechanisms of resistance: clinical and environmental impact]Lorenzo Drago
Microbiologia Clinica, DISP LITA Universita di Milano, Italy
Infez Med 15:6-12. 2007..In vitro studies have shown that, compared to monotherapy with a single antibiotic, synergistic combinations of antibiotics lead to significantly less selection of resistant strains of both P. aeruginosa and MRSA...
[Resistance of Pseudomonas aeruginosa to ciprofloxacin and levofloxacin: 1998-2002]Elena De Vecchi
Laboratorio di Microbiologia, Dipartimento di Scienze Cliniche L. Sacco, Universita degli Studi, Milano, Italy
Infez Med 11:196-200. 2003..Moreover, in contrast with international worldwide studies, resistance rates to ciprofloxacin remained higher than those related to levofloxacin throughout the period studied...
Activity of ceftibuten, cefaclor, azithromycin, clarithromycin, erythromycin and telithromycin against Streptococcus pyogenes clinical isolates with different genotypes and phenotypesLorenzo Drago
Department of Molecular Cellular Animal Biology, University of Camerino, Italy
Chemotherapy 51:268-71. 2005..CONCLUSION: Oral cephalosporins retain the best activity against S. pyogenes; showing good activity except for cMLS-resistant strains, telithromycin is a valid alternative to these antimicrobials...
[Propolis' antimicrobial activity: what's new?]Elena De Vecchi
Laboratorio Microbiologia, Dipartimento Scienze Precliniche LITA Vialba, Universita di Milano, Italy
Infez Med 15:7-15. 2007..This paper reviews the antimicrobial properties of propolis, focusing on respiratory pathogens. These characteristics make propolis a valid option for therapy of upper respiratory tract infections...
Real-time PCR assay for rapid detection of Bacillus anthracis spores in clinical samplesLorenzo Drago
J Clin Microbiol 40:4399. 2002
