Research Topics
| G CoticchioSummaryAffiliation: Tecnobios Procreazione Country: Italy Publications
| Collaborators
|
Detail Information
Publications
Cryopreservation of human oocytesG Coticchio
Tecnobios Procreazione, Centre for Reproductive Health, Via Dante 15, 40125 Bologna, Italy
Hum Fertil (Camb) 4:152-7. 2001..Therefore, it is reasonable to envisage that appropriate modification of current freezing techniques will increase survival rates and make oocyte storage an important option in IVF treatment...- Vitrification may increase the rate of chromosome misalignment in the metaphase II spindle of human mature oocytesG Coticchio
Tecnobios Procreazione, Centre for Reproductive Health, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 19:29-34. 2009..4 +/- 2.6 microm) (P = 0.001). Therefore, under the conditions tested, vitrified-warmed oocytes maintain a MII spindle with a bipolar organization. However, chromosome alignment appears to be partly compromised... - Truths and myths of oocyte sensitivity to controlled rate freezingG Coticchio
Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 15:24-30. 2007.... - Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopyGiovanni Coticchio
Center for Reproductive Health, Tecnobios Procreazione, Bologna, Italy
Fertil Steril 93:2056-64. 2010..To determine whether Polscope analysis can predict different spindle and chromosome configurations of the oocyte metaphase II (MII) spindle... - Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservationG Coticchio
Tecnobios Procreazione, Bologna, Italy
Hum Reprod 21:1771-6. 2006.... - Criteria to assess human oocyte quality after cryopreservationG Coticchio
Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 11:421-7. 2005..In summary, encouraging advances have been made in the field of oocyte cryopreservation, but presently no protocol can ensure standards of success and safety comparable to those guaranteed by embryo storage... - Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentrationV Bianchi
Tecnobios Procreazione, Via Dante 15, 40125 Bologna and University of Bologna, 40125 Bologna, Italy
Hum Reprod 20:1078-83. 2005.... - Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentrationA Borini
Tecnobios Procreazione, Via Dante 15 University of Bologna, 40125 Bologna, Italy
Hum Reprod 21:512-7. 2006..CONCLUSIONS: Our data indicate that although the combination of slow cooling and high sucrose concentration ensures high rates of oocyte survival, it is not sufficient to guarantee a high standard of clinical efficiency... - Evidence-based clinical outcome of oocyte slow coolingA Borini
Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 15:175-81. 2007..4%. Oocyte cryopreservation can contribute considerably to the overall clinical success, ensuring a cumulative rate approaching that achievable with embryo storage... - A PolScope evaluation of meiotic spindle dynamics in frozen-thawed oocytesE Sereni
Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 19:191-7. 2009..These parameters increased after 1 h, although were not restored to the value observed before freezing. No significant changes were observed by extending the culture to 2 h... - Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ARTA Borini
Tecnobios Procreazione, Centre for Reproductive Health, Via Dante 15, Bologna, Italy
Hum Reprod 21:2876-81. 2006.... - Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterolG Coticchio
Tecnobios Procreazione, Bologna, 40125, Italy
Hum Reprod 19:2913-8. 2004..It also appears that concomitant regulation of intra-oocyte cAMP degradation is a prerequisite for meiosis resumption... - Differential sucrose concentration during dehydration (0.2 mol/l) and rehydration (0.3 mol/l) increases the implantation rate of frozen human oocytesV Bianchi
Tecnobios Procreazione, Via Dante 15, 40125 Bologna, Italy
Reprod Biomed Online 14:64-71. 2007..Increased oocyte survival rates can be achieved by moderately high sucrose concentrations in the freezing and thawing solutions. This also ensures elevated success rates in terms of fertilization, embryo development and clinical outcome... - Oocyte cryopreservation: a biological perspectiveG Coticchio
Giovanni Coticchio, Tecnobios Procreazione, Via Dante 15, University of Bologna, 40125 Bologna, Italy
Eur J Obstet Gynecol Reprod Biol 115:S2-7. 2004..Oocyte cryopreservation remains a realistic objective, provided that more systematic approaches are applied, such as thorough analysis of the plasmalemma permeability to water and the diverse cryoprotectants... - Half-dose depot triptorelin in pituitary suppression for multiple ovarian stimulation in assisted reproduction technology: a randomized studyL Dal Prato
Tecnobios Procreazione, Center for Reproductive Health, Via Dante 15, I-40125 Bologna, Italy
Hum Reprod 19:2200-5. 2004..4%) rate was significantly higher in group 1. CONCLUSION: A half-dose of depot triptorelin can be successfully used in ovarian stimulation for IVF and produce a higher number of good quality embryos with a good chance of implantation... - Volume changes of mature human oocytes on exposure to cryoprotectant solutions used in slow cooling proceduresS J Paynter
Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, UK
Hum Reprod 20:1194-9. 2005..Modifications to cryopreservation protocols have not been based on measurement of the osmotic response of oocytes, and methodologies are often poorly described or protocols not strictly adhered to, inevitably resulting in variability... - Ultrastructure of human mature oocytes after slow cooling cryopreservation with ethylene glycolS A Nottola
Department of Anatomy, Sapienza University, Rome 00161, Italy
Reprod Biomed Online 17:368-77. 2008..Thus, the ethylene glycol-based protocol of slow cooling herein described does not seem to offer significant advantages in terms of oocyte structural preservation... - Ultrastructure of human mature oocytes after slow cooling cryopreservation using different sucrose concentrationsS A Nottola
Department of Anatomy, University La Sapienza, Rome, Italy
Hum Reprod 22:1123-33. 2007..We studied the ultrastructural characteristics of human mature oocytes frozen/thawed (F/T) using different concentrations of sucrose. Fresh human mature oocytes were used as controls... - Permeability of human oocytes to ethylene glycol and their survival and spindle configurations after slow cooling cryopreservationL De Santis
IVF Unit, Vita Salute University, H S Raffaele, Milan, Italy
Hum Reprod 22:2776-83. 2007.... - Objective evaluation of the viability of cryopreserved oocytesL De Santis
Vita Salute University, IVF Unit, H S Raffaele, Via Olgettina 60, 20132 Milan, Italy
Reprod Biomed Online 15:338-45. 2007..Some progress has been made, however, as suggested by recent experiences in which the implantation efficiency of embryos produced from thawed oocytes approaches that reported using cryopreserved embryos directly...