Research Topics
Genomes and Genes
| Giulio CossuSummaryAffiliation: San Raffaele Scientific Institute Country: Italy Publications
| Collaborators
|
Detail Information
Publications
Mesoangioblast stem cells ameliorate muscle function in dystrophic dogsMaurilio Sampaolesi
San Raffaele Scientific Institute, , Stem Cell Research Institute, Via Olgettina 58, 20132 Milan, Italy
Nature 444:574-9. 2006..The outcome is a remarkable clinical amelioration and preservation of active motility. These data qualify mesoangioblasts as candidates for future stem cell therapy for Duchenne patients...- Induced early expression of mrf4 but not myog rescues myogenesis in the myod/myf5 double-morphant zebrafish embryoEsther Schnapp
Stem Cell Research Institute, DIBIT, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy
J Cell Sci 122:481-8. 2009..Therefore our data also show that the early myotome is essential for subsequent skeletal muscle differentiation and patterning in the zebrafish... - Fusion of bone marrow-derived stem cells with striated muscle may not be sufficient to activate muscle genesGiulio Cossu
Stem Cell Research Institute, DIBIT, Milan, Italy
J Clin Invest 114:1540-3. 2004..However, these cells fail to express sarcoglycan and thus to repair the tissue, which suggests that this stem cell population has limited potential for cardiac and skeletal muscle regeneration... - New therapies for muscular dystrophy: cautious optimismGiulio Cossu
Stem Cell Research Institute, DIBIT, H San Raffaele, 58 Via Olgettina, 20132 Milan, Italy
Trends Mol Med 10:516-20. 2004..Importantly, a combination of these strategies might greatly enhance the possibility of successful therapy... - Oriented cell divisions and muscle satellite cell heterogeneityGiulio Cossu
Stem Cell Research Institute, DIBIT, San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Cell 129:859-61. 2007..They show that asymmetric division of stem cells in the satellite cell niche is a mechanism for generating these two populations... - New therapies for Duchenne muscular dystrophy: challenges, prospects and clinical trialsGiulio Cossu
Stem Cell Research Institute, DIBIT, H San Raffaele, 58 Via Olgettina, 20132 Milan, Italy
Trends Mol Med 13:520-6. 2007..Here we review the main experimental strategies, namely drug, gene and cell therapies, outlining their goals and limitations. We also provide an update of ongoing or planned clinical trials based on these strategies... - Satellite cells, myoblasts and other occasional myogenic progenitors: possible origin, phenotypic features and role in muscle regenerationGiulio Cossu
Stem Cell Research Institute, DIBIT, H San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Semin Cell Dev Biol 16:623-31. 2005..Finally, possible use of different myogenic cells in experimental protocols of cell therapy will be briefly outlined... - Skeletal muscle differentiation of embryonic mesoangioblasts requires pax3 activityGraziella Messina
Stem Cell Research Institute, H San Raffaele Scientific Institute, Milan, Italy
Stem Cells 27:157-64. 2009..These data demonstrate that Pax3 is required for the differentiation of mesoangioblast stem cells into skeletal muscle, in keeping with its role in orchestrating entry into the myogenic program... - Ex vivo treatment with nitric oxide increases mesoangioblast therapeutic efficacy in muscular dystrophyClara Sciorati
Stem Cell Research Institute, H San Raffaele Scientific Institute, 20132, Milan, Italy
J Cell Sci 119:5114-23. 2006..We conclude that NO donors exert multiple beneficial effects on mesoangioblasts that may be used to increase their efficacy in cell therapy of muscular dystrophies... - Intrinsic phenotypic diversity of embryonic and fetal myoblasts is revealed by genome-wide gene expression analysis on purified cellsStefano Biressi
Stem Cell Research Institute, DIBIT, H San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Dev Biol 304:633-51. 2007.... - Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cellsArianna Dellavalle
Stem Cell Research Institute, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy
Nat Cell Biol 9:255-67. 2007.... - Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferationRoberta Palumbo
Department of Molecular Biology and Functional Genomics, San Raffaele Research Institute, Milan, Italy
J Cell Biol 164:441-9. 2004..Although the role of endogenous HMGB1 in the reconstruction of dystrophic muscle remains to be clarified, injected HMGB1 may be used to promote tissue regeneration... - Human cardiac mesoangioblasts isolated from hypertrophic cardiomyopathies are greatly reduced in proliferation and differentiation potencyBeatriz G Galvez
Division of Regenerative Medicine, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy
Cardiovasc Res 83:707-16. 2009..Our objective was to test whether progenitor cell proliferation and differentiation potential may vary depending upon the disease of the donor... - Embryonic stem-derived versus somatic neural stem cells: a comparative analysis of their developmental potential and molecular phenotypeElena Colombo
Stem Cell Research Department, Dipartmento di Biotecnologie, San Raffaele Scientific Institute, Via Olgettina 58, 20132 Milan, Italy
Stem Cells 24:825-34. 2006..This may contribute to the high proliferation rate exhibited by these cells in culture... - Nfix regulates fetal-specific transcription in developing skeletal muscleGraziella Messina
San Raffaele Scientific Institute, Milan, Italy
Cell 140:554-66. 2010..Therefore, Nfix acts as a transcriptional switch from embryonic to fetal myogenesis... - Isolation and characterization of mesoangioblasts from mouse, dog, and human tissuesRossana Tonlorenzi
Stem Cell Research Institute, San Raffaele Scientific Institute, Milan, Italy
Curr Protoc Stem Cell Biol . 2007..This unit provides detailed methods for isolation, culture, and characterization of mesoangioblasts... - Complete repair of dystrophic skeletal muscle by mesoangioblasts with enhanced migration abilityBeatriz G Galvez
Stem Cell Research Institute, San Raffaele Hospital, 20132 Milan, and Department of Experimental Medicine, Human Anatomy Institute, University of Pavia, Italy
J Cell Biol 174:231-43. 2006..This study defines the requirements for efficient engraftment of mesoangioblasts and offers a new potent tool to optimize future cell therapy protocols for muscular dystrophies... - Mouse orthologue of ARX, a gene mutated in several X-linked forms of mental retardation and epilepsy, is a marker of adult neural stem cells and forebrain GABAergic neuronsElena Colombo
Stem Cell Research Institute, DIBIT, San Raffaele Science Park, Via Olgettina 58, 20132 Milan, Italy
Dev Dyn 231:631-9. 2004..In conclusion, our mouse Arx expression data provide important further insights into the puzzling complexity of the human ARX mutation pleiotropy... - Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscleLuigi Anastasia
Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, L.I.T.A. via F.lli Cervi 93, 20090 Segrate, Milan, Italy
Cell Death Differ 13:2042-51. 2006..Moreover, this is the first study to demonstrate that plasticity changes arise in primary mouse and human cells following reversine exposure... - An evolutionarily acquired genotoxic response discriminates MyoD from Myf5, and differentially regulates hypaxial and epaxial myogenesisAnna Innocenzi
Division of Regenerative Medicine, San Raffaele Scientific Institute, Via Olgettina 58, Milan 20132, Italy
EMBO Rep 12:164-71. 2011.... - Cells migrating to sites of tissue damage in response to the danger signal HMGB1 require NF-kappaB activationRoberta Palumbo
Chromatin Dynamics Unit, Stem Cell Research Institute, Istituto Scientifico San Raffaele, 20132 Milan, Italy
J Cell Biol 179:33-40. 2007..These findings suggest that NF-kappaB signaling controls tissue regeneration in addition to early events in inflammation... - Mesoangioblasts, vessel-associated multipotent stem cells, repair the infarcted heart by multiple cellular mechanisms: a comparison with bone marrow progenitors, fibroblasts, and endothelial cellsDaniela Galli
Stem Cell Research Institute, DIBIT, H. San Raffaele, Milan, Italy
Arterioscler Thromb Vasc Biol 25:692-7. 2005..CONCLUSIONS: Mabs appear as effective as BMPCs in reducing postinfarction LV dysfunction, likely through production of antiapoptotic and angiogenic factors... - A role for MSX2 and necdin in smooth muscle differentiation of mesoangioblasts and other mesoderm progenitor cellsSilvia Brunelli
Stem Cell Research Institute, Dibit-H. San Raffaele, Milan, Italy
Trends Cardiovasc Med 15:96-100. 2005.... - Stem cell-mediated transfer of a human artificial chromosome ameliorates muscular dystrophyFrancesco Saverio Tedesco
Division of Regenerative Medicine, Stem Cells and Gene Therapy, San Raffaele Scientific Institute, 20132 Milan, Italy
Sci Transl Med 3:96ra78. 2011..Thus, HAC-mediated gene transfer shows efficacy in a preclinical model of DMD and offers potential for future clinical translation... - Beta catenin-independent activation of MyoD in presomitic mesoderm requires PKC and depends on Pax3 transcriptional activitySilvia Brunelli
Stem Cell Research Institute, H San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milan, Italy
Dev Biol 304:604-14. 2007.... - Repairing skeletal muscle: regenerative potential of skeletal muscle stem cellsFrancesco Saverio Tedesco
Division of Regenerative Medicine, San Raffaele Scientific Institute, 58 Via Olgettina, Milan, Italy
J Clin Invest 120:11-9. 2010..Clinical trials utilizing these cells to treat muscular dystrophies, heart failure, and stress urinary incontinence are also briefly outlined... - Msx2 and necdin combined activities are required for smooth muscle differentiation in mesoangioblast stem cellsSilvia Brunelli
Stem Cell Research Institute, DIBIT H, Milan, Italy
Circ Res 94:1571-8. 2004..These data support the hypothesis that Msx2 and necdin act as master genes regulating SM differentiation in at least a subset of SMCs... - Cellular heterogeneity during vertebrate skeletal muscle developmentStefano Biressi
Stem Cell Research Institute, DIBIT, San Raffaele Scientific Institute, 58 Via Olgettina, 20132 Milan, Italy
Dev Biol 308:281-93. 2007..Recent cell lineage and gene expression studies have expanded our knowledge on how skeletal muscle is formed and how its heterogeneity is generated. This review will present a comprehensive view of relevant findings in this field... - Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblastsMaurilio Sampaolesi
Stem Cell Research Institute, H. S. Raffaele, Via Olgettina 58, 20132 Milan, Italy
Science 301:487-92. 2003..The success of this protocol was mainly due to widespread distribution of donor stem cells through the capillary network, a distinct advantage of this strategy over previous approaches... - The meso-angioblast: a multipotent, self-renewing cell that originates from the dorsal aorta and differentiates into most mesodermal tissuesMaria G Minasi
Stem Cell Research Institute, DIBIT, H S Raffaele, Via Olgettina 58, 20132 Milano, Italy
Development 129:2773-83. 2002.... - Correlation of circulating CD133+ progenitor subclasses with a mild phenotype in Duchenne muscular dystrophy patientsChiara Marchesi
Stem Cell Laboratory, Department of Neurological Science, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Milan, Italy
PLoS ONE 3:e2218. 2008..Here we assessed whether the levels of certain stem cells may predict the progression of Duchenne muscular dystrophy (DMD)... - The origin of embryonic and fetal myoblasts: a role of Pax3 and Pax7Graziella Messina
Division of Regenerative Medicine, San Raffaele Scientific Institute, Milan, Italy
Genes Dev 23:902-5. 2009..embryonic and fetal myoblasts and satellite cells whose origin and lineage relationship have been elusive so far. In this issue of Genes & Development, Hutcheson and colleagues (pp. 997-1013) provide a first answer to this question... - Follistatin induction by nitric oxide through cyclic GMP: a tightly regulated signaling pathway that controls myoblast fusionAddolorata Pisconti
Stem Cell Research Institute, Hospital San Raffaele, 20132 Milan, Italy
J Cell Biol 172:233-44. 2006..These results establish a hitherto unappreciated role of NO and cGMP in regulating myoblast fusion and elucidate their mechanism of action, providing a direct link with follistatin, which is a key player in myogenesis... - Skeletal myogenic progenitors in the endothelium of lung and yolk sacMaria Gabriella Cusella De Angelis
Department of Experimental Medicine, University of Pavia, Pavia, Italy
Exp Cell Res 290:207-16. 2003..The number (or potency to generate skeletal muscle) of these vessels associated cells decreases rapidly with age and is very low in mature animals, possibly correlating with reduced regenerative capacity of adult mammalian tissues... - The Wnt/beta-catenin pathway regulates Gli-mediated Myf5 expression during somitogenesisUgo Borello
Stem Cell Research Institute, DIBIT, H San Raffaele, Via Olgettina 58, 20132 Milan, Italy
Development 133:3723-32. 2006..Taken together, these results demonstrate that Myf5 is a direct target of Wnt/beta-catenin, and that its full activation requires a cooperative interaction between the canonical Wnt and the Shh/Gli pathways in muscle progenitor cells... - Bhlhb5 is expressed in the CNS and sensory organs during mouse embryonic developmentSilvia Brunelli
Stem Cell Research Institute, DiBiT HSR, Via Olgettina 58, 20132, Milan, Italy
Gene Expr Patterns 3:755-9. 2003..In addition Bhlhb5 is expressed in the developing eye and hair follicles, in the epithelial layer of the cochlea in the developing inner, and in the nasal epithelium... - Bsx, an evolutionary conserved Brain Specific homeoboX gene expressed in the septum, epiphysis, mammillary bodies and arcuate nucleusMattia Cremona
Stem Cell Research Institute, DIBIT, San Raffaele Science Park Via Olgettina 58, Milan 20132, Italy
Gene Expr Patterns 4:47-51. 2004..In particular, Bsx might be considered an important molecular marker for early embryonic stages of epiphysis development, being specifically expressed in this neural structure from E9.5 onwards... - Neural stem cells: plasticity and their transdifferentiation potentialAngelo Vescovi
Stem Cell Research Institute, DIBIT H San Raffaele, Milan, Italy
Cells Tissues Organs 171:64-76. 2002..In this article, we review some of the basic notions regarding the functional properties of the adult neural stem cells and discuss findings in the expanding area of trans-germ layer conversion, with emphasis on the neural stem cell... - Myogenic potential of mouse primordial germ cellsFrancesca-Gioia Klinger
, , Roma, Italy
Int J Dev Biol 47:303-5. 2003..This occurs at an estimated frequency of 0.01%, which is no higher than that reported for stem cells of adult tissues... - PlGF-MMP-9-expressing cells restore microcirculation and efficacy of cell therapy in aged dystrophic muscleCesare Gargioli
San Raffaele Biomedical Park Foundation of Rome, 100 Via Castel Romano, 10028 Rome, Italy
Nat Med 14:973-8. 2008..These data open the possibility of extending new therapies to currently untreatable individuals... - A highly conserved molecular switch binds MSY-3 to regulate myogenin repression in postnatal muscleLibera Berghella
Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical Science Park, Rome 00128, Italy
Genes Dev 22:2125-38. 2008.... - REN: a novel, developmentally regulated gene that promotes neural cell differentiationRita Gallo
Department of Experimental Medicine, University of L Aquila, 67100 L Aquila, Italy
J Cell Biol 158:731-40. 2002..We propose that REN represents a novel component of the neurogenic signaling cascade induced by retinoic acid, EGF, and NGF, and is both a marker and a regulator of neuronal differentiation... - Human circulating AC133(+) stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscleYvan Torrente
Stem Cell Laboratory, Department of Neurological Science, Instituto di Ricovero e Cura a Carattere Scientifico Ospedale Maggiore Policlinico, Centro Dino Ferrari, University of Milan, Italy
J Clin Invest 114:182-95. 2004..As these cells can be isolated from the blood, manipulated in vitro, and delivered through the circulation, they represent a possible tool for future cell therapy applications in DMD disease or other muscular dystrophies... - Isolation and characterization of mesoangioblasts from facioscapulohumeral muscular dystrophy muscle biopsiesRoberta Morosetti
Department of Neurosciences, Catholic University School of Medicine, Largo A Gemelli 8, 00168 Rome, Italy
Stem Cells 25:3173-82. 2007..Disclosure of potential conflicts of interest is found at the end of this article... - Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiationDaniela Deponti
Department of Histology and Medical Embryology, University of Roma La Sapienza, 00161 Rome, Italy
J Cell Biol 179:305-19. 2007..Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration... - Isolation and expansion of adult cardiac stem cells from human and murine heartElisa Messina
Department of Experimental Medicine and Pathology, University La Sapienza, Rome, Italy
Circ Res 95:911-21. 2004.... - Smooth muscle of the dorsal aorta shares a common clonal origin with skeletal muscle of the myotomeMilan Esner
CNRS URA 2578, Department of Developmental Biology, Pasteur Institute, 28 rue du Dr Roux, 75 724 Paris Cedex 15, France
Development 133:737-49. 2006..These results are discussed in terms of the paraxial mesoderm contribution to the aorta and of the mesoangioblast stem cells that derive from it... - TGFbeta/BMP activate the smooth muscle/bone differentiation programs in mesoangioblastsEnrico Tagliafico
Dipartimento di Scienze Biomediche, , Via G. Campi 287, 41100 Modena, Italy
J Cell Sci 117:4377-88. 2004..Our data define a unique phenotype for mesoangioblasts, explain several of their biological features and set the basis for future functional studies on the role of these cells in tissue histogenesis and repair... - Fusion-independent expression of functional ACh receptors in mouse mesoangioblast stem cells contacting muscle cellsFrancesca Grassi
Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia and Centro di Eccellenza BEMM, Università La Sapienza Piazzale Aldo Moro 5, I 00185 Roma, Italy
J Physiol 560:479-89. 2004.... - Allogeneic mesoangioblasts give rise to alpha-sarcoglycan expressing fibers when transplanted into dystrophic miceMaria Guttinger
Institute for Cell Biology and Tissue Engineering, Fondazione Parco Biomedico San Raffaele, Via Castel Romano 100, 10028 Rome, Italy
Exp Cell Res 312:3872-9. 2006.... - MyoD expression restores defective myogenic differentiation of human mesoangioblasts from inclusion-body myositis muscleRoberta Morosetti
Department of Neurosciences and Interdisciplinary Laboratory for Stem Cell Research and Cellular Therapy, Catholic University, Largo A. Gemelli 8, 00168 Rome, Italy
Proc Natl Acad Sci U S A 103:16995-7000. 2006..Indeed, silencing this gene or overexpressing MyoD rescues the myogenic defect of IBM mesoangioblasts, opening novel cell-based therapeutic strategies for this crippling disorder... - Nitric oxide release combined with nonsteroidal antiinflammatory activity prevents muscular dystrophy pathology and enhances stem cell therapySilvia Brunelli
Department of Experimental Medicine, University of Milano Bicocca, 20052 Monza, Italy
Proc Natl Acad Sci U S A 104:264-9. 2007.... - Regulation of vertebrate myotome development by the p38 MAP kinase-MEF2 signaling pathwayLuciana De Angelis
Istituto di Istologia ed Embriologia Generale, Universita di Roma La Sapienza, Via A Scarpa 14, 00161 Rome, Italy
Dev Biol 283:171-9. 2005..Thus, we present novel evidence indicating a crucial role for p38 MAPK signaling to the MEF2 transcriptional regulators during early mammalian somite development and myotome formation... - Stem and progenitor cells in skeletal muscle development, maintenance, and therapyBruno Peault
Stem Cell Research Center, Children s Hospital of Pittsburgh, Department of Orthopaedic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA
Mol Ther 15:867-77. 2007..As an alternative to myoblast transplantation, stem cells such as mesoangioblasts and CD133+ progenitors administered through blood circulation have recently shown great potential to regenerate dystrophic muscle... - Mesoangioblasts--vascular progenitors for extravascular mesodermal tissuesGiulio Cossu
Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical Science Park of Rome, Via Castel Romano 100, 00128 Rome, Italy
Curr Opin Genet Dev 13:537-42. 2003.... - Sphingosine 1-phosphate mediates proliferation and survival of mesoangioblastsChiara Donati
Dipartimento di Scienze Biochimiche and Instituto Interuniversitario di Miotogia, Universita di Firenze, Viale G B Morgagni 50, 50134 Firenze, Italy
Stem Cells 25:1713-9. 2007..Disclosure of potential conflicts of interest is found at the end of this article... - Stage-specific modulation of skeletal myogenesis by inhibitors of nuclear deacetylasesSimona Iezzi
Laboratory of Muscle Biology, Muscle Gene Expression Group, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Proc Natl Acad Sci U S A 99:7757-62. 2002..These results represent the first evidence that DIs can enhance muscle differentiation and suggest the rationale for their use in manipulating adult and embryonic skeletal myogenesis... - Chimeric snRNA molecules carrying antisense sequences against the splice junctions of exon 51 of the dystrophin pre-mRNA induce exon skipping and restoration of a dystrophin synthesis in Delta 48-50 DMD cellsFernanda Gabriella De Angelis
Institute Pasteur Fondazione Cenci-Bolognetti, Department of Genetics and Molecular Biology, University La Sapienza, P.le Aldo Moro 5, 00185 Rome, Italy
Proc Natl Acad Sci U S A 99:9456-61. 2002..We also show that the highest skipping activity was found when antisense constructs against the 5' and 3' splice sites are coexpressed in the same cell... - Binding of sFRP-3 to EGF in the extra-cellular space affects proliferation, differentiation and morphogenetic events regulated by the two moleculesRaffaella Scardigli
Department of Developmental Biology, Institute of Cell Biology and Tissue Engineering, San Raffaele Biomedical Science Park of Rome, Rome, Italy
PLoS ONE 3:e2471. 2008..Injection of sFRP-3 mRNA blocks expression of XMyoD mRNA and leads to embryos with enlarged heads and shortened trunks... - Electrospun degradable polyesterurethane membranes: potential scaffolds for skeletal muscle tissue engineeringStefania A Riboldi
Department of Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci, 32, 20133 Milano, Italy
Biomaterials 26:4606-15. 2005....