An evaluation of Comparative Genome Sequencing (CGS) by comparing two previously-sequenced bacterial genomesChristopher D Herring
Department of Bioengineering, University of California San Diego, La Jolla, USA
BMC Genomics 8:274. 2007
..It is critical though to know the accuracy of the technique used, and to establish confidence that all of the mutations were detected...
Systems approach to refining genome annotationJennifer L Reed
Department of Bioengineering, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0412, USA
Proc Natl Acad Sci U S A 103:17480-4. 2006
..This study thus demonstrates the use of systems analysis to discover metabolic and transport functions and their genetic basis by a combination of experimental and computational approaches...
The global transcriptional regulatory network for metabolism in Escherichia coli exhibits few dominant functional statesChristian L Barrett
Bioengineering Department, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0412, USA
Proc Natl Acad Sci U S A 102:19103-8. 2005
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Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescaleChristopher D Herring
Department of Bioengineering, University of California, San Diego, California 92093, USA
Nat Genet 38:1406-12. 2006
..The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts...
Marker removal system for Thermoanaerobacterium saccharolyticum and development of a markerless ethanologenA Joe Shaw
Mascoma Corporation, 67 Etna Rd, Lebanon, NH 03766, USA
Appl Environ Microbiol 77:2534-6. 2011
..The pta- and ack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless ?ldh ?pta ?ack strain that produces ethanol at a high yield...
Immobilization of Escherichia coli RNA polymerase and location of binding sites by use of chromatin immunoprecipitation and microarraysChristopher D Herring
Department of Engineering, UC San Diego Bioengineering, 9500 Gilman Drive, Dept 0412, La Jolla, CA 92093-0412, USA
J Bacteriol 187:6166-74. 2005
..This map of RNA polymerase binding sites represents a foundation for studies of transcription factors in E. coli and an important evaluation of the chIP-chip technique...
In silico design and adaptive evolution of Escherichia coli for production of lactic acidStephen S Fong
Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0412, USA
Biotechnol Bioeng 91:643-8. 2005
..Strains grown on 2 g/L glucose at 37 degrees C showed lactate titers ranging from 0.87 to 1.75 g/L and secretion rates that were directly coupled to growth rates...
Introduction of conditional lethal amber mutations in Escherichia coliChristopher D Herring
Mascoma Corporation, Lebanon, NH, USA
Methods Mol Biol 416:323-34. 2008
..The mutants then depend upon expression of a suppressor transfer RNA (tRNA) for survival, which is expressed under control of the arabinose promoter on a high-copy-number plasmid...
Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmRChristopher D Herring
Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
J Bacteriol 186:6714-20. 2004
..The operon was shown to be induced by formaldehyde, and the genes have been renamed frmR, frmA, and frmB, respectively...
Conditional lethal amber mutations in essential Escherichia coli genesChristopher D Herring
Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
J Bacteriol 186:2673-81. 2004
..Such experiments may inexpensively provide valuable information for the identification and prioritization of targets for antibiotic development...
Gene replacement without selection: regulated suppression of amber mutations in Escherichia coliChristopher D Herring
Laboratory of Genetics, University of Wisconsin Madison, 445 Henry Mall, Madison, WI 53706, USA
Gene 311:153-63. 2003
..To compliment this approach we developed an arabinose inducible amber suppressor tRNA. Controlled expression mediated by the suppressor was demonstrated for the lacZ and xylA amber mutants...
Engineering a reduced Escherichia coli genomeVitaliy Kolisnychenko
Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
Genome Res 12:640-7. 2002
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