Research Topics
| Evangelia Vretou (aka Jockers-Wretou)SummaryAffiliation: Hellenic Pasteur Institute Location: Athens, Greece Publications
| Collaborators
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Detail Information
Publications
Identification and characterization of Inc766, an inclusion membrane protein in Chlamydophila abortus-infected cellsEvangelia Vretou
Laboratory of Biotechnology, Department of Microbiology, Hellenic Pasteur Institute, 127 Vassilissis Sofias Avenue, 11521 Athens, Greece
Microb Pathog 45:265-72. 2008..Future studies of the particular characteristics especially the interactive properties of Inc766 should contribute to our understanding of the relationship of the different chlamydial species with their respective hosts...- Genome sequence of the Chlamydophila abortus variant strain LLGMichelle Sait
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, United Kingdom
J Bacteriol 193:4276-7. 2011..Genome sequencing revealed differences between LLG and S26/3 to occur in pseudogene content, in transmembrane head/inc family proteins, and in biotin biosynthesis genes... - [Ovine enzootic abortion: seroprevalence in Switzerland using a competitive enzyme-linked immunosorbent assay (cELISA)]N Borel
Institut für Veterinärpathologie der Universität Zürich
Schweiz Arch Tierheilkd 144:474-82. 2002..Higher sensitivity (92.9%) and specificity (97.6%) than the complement fixation test (CFT) in combination with testing of pooled sera makes the cELISA to be an usuable tool for serological screening on flock level... - Serological diagnosis of ovine enzootic abortion by enzyme-linked immunosorbent assay with a recombinant protein fragment of the polymorphic outer membrane protein POMP90 of Chlamydophila abortusDavid Longbottom
Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik EH26 0PZ, United Kingdom
J Clin Microbiol 40:4235-43. 2002..Overall, the new rOMP90-4 ELISA was found to be a more sensitive and specific test than CFT for differentiating animals infected with C. abortus from those infected with C. pecorum... - Polymorphic outer-membrane proteins of Chlamydophila abortus are glycosylatedE Vretou
Department of Microbiology, Hellenic Pasteur Institute, Vassilissis Sofias 127, Athens 11521, Greece
Microbiology 147:3303-10. 2001..A possible role for the N-linked oligosaccharides in the POMPs might be the promotion of the proper folding and processing of these proteins... - Experimental infection of pregnant ewes with enteric and abortion-source Chlamydophila abortusP Tsakos
Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University, 54 006, Thessaloniki, Greece
Vet Microbiol 82:285-91. 2001..Our results suggest that enteric C. abortus can be associated with placental and foetal lesions in sheep... - Diagnosis of ovine enzootic abortion using an indirect ELISA (rOMP91B iELISA) based on a recombinant protein fragment of the polymorphic outer membrane protein POMP91B of Chlamydophila abortusD Longbottom
Moredun Research Institute, International Research Centre, Pentlands Science Park, Penicuik, Midlothian, UK
FEMS Microbiol Lett 195:157-61. 2001..5%) than the CFT. Furthermore, the iELISA was better at differentiating C. abortus- from C. pecorum-infected animals. The new rOMP91B iELISA test will prove a valuable tool for the routine serodiagnosis of C. abortus infection... - Identification of protective epitopes by sequencing of the major outer membrane protein gene of a variant strain of Chlamydia psittaci serotype 1 (Chlamydophila abortus)E Vretou
Department of Microbiology, Hellenic Pasteur Institute, Athens 115 21, Greece
Infect Immun 69:607-12. 2001..The results suggest that multiple sites located in the three VSs contribute to the epitope of protective MAbs... - Molecular characterization of a bacteriophage (Chp2) from Chlamydia psittaciB L Liu
Department of Molecular Microbiology, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom
J Virol 74:3464-9. 2000..We suggest that VP3 performs a scaffolding-like function but has evolved into a structural protein... - [Chlamydia abortion in sheep: possibilities for serological diagnosis using a competitive ELISA and insight into the epidemiologic situation in Switzerland]P Gut-Zangger
Institut für Veterinärpathologie, Universitat Zurich
Schweiz Arch Tierheilkd 141:361-6. 1999..The complement fixation test (CFT), still considered as standard method for serological examination for Chlamydiae, has additionally been applied... - T2 relaxation time study of iron overload in b-thalassemiaS I Mavrogeni
Onassis Cardiac Surgery Center, Athens, Greece
MAGMA 6:7-12. 1998.... - Immunoelectron microscopic localisation of the OMP90 family on the outer membrane surface of Chlamydia psittaciD Longbottom
Moredun Research Institute, International Research Centre, Penicuik, Midlothian, UK
FEMS Microbiol Lett 164:111-7. 1998..Surface localisation on elementary bodies indicates the importance of these proteins as protective antigen candidates... - Antigenic organization of the N-terminal part of the polymorphic outer membrane proteins 90, 91A, and 91B of Chlamydophila abortusEvangelia Vretou
Laboratory of Biotechnology, Department of Microbiology, Hellenic Pasteur Institute, Athens 115 21, Greece
Infect Immun 71:3240-50. 2003..The results presented in this study provide some evidence for a passenger domain that is folded as a beta-helix pyramid with compact antigenic organization... - Seroprevalences for ovine enzootic abortion in SwitzerlandN Borel
Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 268, CH 8057 Zurich, Switzerland
Prev Vet Med 65:205-16. 2004..The cELISA in combination with testing pooled sera and statistical methods for true prevalence estimation provided a good survey tool at lower costs and time when compared to other approaches... - Chlamydia trachomatis ompA genotypes in male patients with urethritis in Greece: conservation of the serovar distribution and evidence for mixed infections with Chlamydophila abortusPanagiotis Psarrakos
Laboratory of Biotechnology, Hellenic Pasteur Institute, Athens, Greece
Mol Cell Probes 25:168-73. 2011..The implications resulting from this observation for the health of the general population are discussed... - Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genesMichelle Sait
Moredun Research Institute, Pentlands Science Park, Bush Loan, Edinburgh, Midlothian EH26 0PZ, UK
Vet Microbiol 151:284-90. 2011..abortus is low, the sequence heterogeneity should be an important consideration when using pmps as the basis for novel diagnostics or vaccine development... - Multi locus sequence typing of Chlamydia reveals an association between Chlamydia psittaci genotypes and host speciesYvonne Pannekoek
Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands
PLoS ONE 5:e14179. 2010..The phylogenetic tree of C. psittaci did not follow that of its host bird species, suggesting host species jumps. In conclusion, we report for the first time an association between C. psittaci genotypes with host species... - Differential identification of Chlamydophila abortus live vaccine strain 1B and C. abortus field isolates by PCR-RFLPKarine Laroucau
Unité Zoonoses Bactériennes, Agence Française de Sécurité Sanitaire des Aliments Lerpaz, 23 Avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France
Vaccine 28:5653-6. 2010..Our results indicate that the three mutations are uniquely present in the vaccine strain, and as such provide easy-to-use markers for the differential identification of the vaccine strain and wild-type isolates... - Genotyping of Chlamydophila abortus strains by multilocus VNTR analysisKarine Laroucau
Unité Zoonoses Bactériennes, Agence Française de Sécurité Sanitaire des Aliments Lerpaz, 94706 Maisons Alfort cedex, France
Vet Microbiol 137:335-44. 2009..abortus isolates of different origin, which is suitable for molecular epidemiological studies... - Recent developments in the laboratory diagnosis of chlamydial infectionsKonrad Sachse
Friedrich Loeffler Institut Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Naumburger Str 96a, 07743 Jena, Germany
Vet Microbiol 135:2-21. 2009..Further, a combination of a specific real-time PCR assay and a microarray test for chlamydiae is proposed as an alternative reference standard to isolation by cell culture... - Ovine Enzootic Abortion (OEA): a comparison of antibody responses in vaccinated and naturally-infected swiss sheep over a two year periodAndrea Gerber
Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Switzerland
BMC Vet Res 3:24. 2007..abortus over a two-year time period... - Evaluation of two commercial assays for the detection of Chlamydophila abortus antibodiesE Vretou
Department of Microbiology, Hellenic Pasteur Institute, 127 Avenue Vassilissis Sofias, 115 21 Athens, Greece
Vet Microbiol 123:153-61. 2007..The C. abortus ELISA by the Institut Pourquier identified less OEA-positive sera than the other assays though it identified correctly 9 of 10 OEA-positive flocks. The basis of the discordant results is discussed... - Ovine enzootic abortion (OEA): antibody response in vaccinated sheep compared to naturally infected sheepN Borel
I Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 268, CH-8057 Zurich, Switzerland
Vet Res Commun 29:151-6. 2005 - Two-dimensional electrophoretic analysis of the protein family at 90 kDa of abortifacient Chlamydia psittaciP Giannikopoulou
Department of Biotechnology, Hellenic Pasteur Institute, Athens, Greece
Electrophoresis 18:2104-8. 1997..The 4-member antigen family at 90/104 kDa is the first example of proteins coded by multiple genes within the genus Chlamydia... - Diagnosis of ovine enzootic abortion, using a competitive ELISA based on monoclonal antibodies against variable segments 1 and 2 of the major outer membrane protein of Chlamydia psittaci serotype 1V Salti-Montesanto
Department of Biotechnology, Hellenic Pasteur Institute, Athens, Greece
Am J Vet Res 58:228-35. 1997..The cELISA was evaluated with field sera, and the results were compared with those obtained by complement-fixation testing and by an ELISA containing solubilized outer membrane complexes (A-ELISA)... - Extensive heterogeneity of the protein composition of Chlamydia trachomatis following serial passage in two different cell linesP C Goswami
Department of Microbiology, St Louis University School of Medicine, MO 63104 1028
J Gen Microbiol 136:1623-9. 1990..Immunoblotting experiments with a rabbit and a human polyclonal sera indicated that distinct host-specified chlamydial proteins were reactive with the two sera... - Host passage-dependent wheat germ agglutinin-binding proteins of Chlamydia trachomatisP C Goswami
Department of Microbiology, St Louis University School of Medicine, MO 63104 1028
FEMS Microbiol Lett 65:53-6. 1991..Some of these WGA-binding proteins in the EB of serovars L1 and E exhibited altered molecular mass after adaptation in the two alternate hosts, HeLa and McCoy cells. These results suggest that WGA-binding proteins exist in chlamydiae... - Eukaryotic cell components that bind to chlamydial elementary bodies: the histonesE Vretou
Department of Biotechnology, Hellenic Pasteur Institute, Athens, Greece
FEMS Microbiol Lett 70:225-30. 1992..These data suggest that the HeLa-cell-binding chlamydial proteins were previously identified by their reaction with chromatin and not with membrane components... - The role of lipopolysaccharide in the exposure of protective antigenic sites on the major outer membrane protein of Chlamydia trachomatisE Vretou
Department of Biotechnology, Institut Pasteur Hellenique, Athens, Greece
J Gen Microbiol 138:1221-7. 1992..They further imply that serovars protected by Mg and those that are not differ in the surface topology of one particular MOMP epitope, but are antigenically very similar. This difference might be of considerable importance in vivo... - Unusual prevalence of the rare serovar Da of Chlamydia trachomatis in Greece detected by monoclonal antibodiesE Vretou
Department of Biotechnology, Institut Pasteur Hellenique, Athens, Greece
Sex Transm Dis 19:78-83. 1992..The results were consistent with the specificity of these mAbs observed with whole microorganisms and indicated their usefulness as epidemiologic markers... - Serum creatine kinase isoenzyme pattern in nervous system atrophies and neuromuscular disordersE Jockers-Wretou
Clin Chim Acta 73:183-6. 1976 - Quantitation of creatine kinase isoenzymes in human tissues and sera by an immunological methodE Jockers-Wretou
Clin Chim Acta 58:223-32. 1975..This indicates that the method is of diagnostic value... - Accuracy of immunological methods for measurement of creatine kinase isoenzyme activitiesE Jockers-Wretou
Clin Chem 24:179-80. 1978 - Atypical serum creatine kinase isoenzyme pattern caused by complexing of creatine kinase-BB with immunoglobulins G and A1),2)E Jockers-Wretou
J Clin Chem Clin Biochem 17:731-7. 1979..The complex occurring in vivo could also be produced in vitro by using purified patients' IgG and human creatine kinase-BB... - Macro-creatine kinase-BB: observations on 6 patientsG Chemnitz
J Clin Chem Clin Biochem 17:725-9. 1979..Both properties are attributable to the binding of creatine kinase-BB to immunoglobulins... - Accessibility of an epitope common to all histone H3 variants in folded and unfolded chromatin as studied by a monoclonal antibodyE Jockers-Wretou
Institut Pasteur Hellenique, Athens, Greece
Mol Biol Rep 13:123-31. 1988.... - Adherence of multiple serovars of Chlamydia trachomatis to a common receptor on HeLa and McCoy cells is mediated by thermolabile protein(s)E Vretou
Department of Microbiology, St Louis University School of Medicine, MO 63104
J Gen Microbiol 135:3229-37. 1989..trachomatis, including that of lymphogranuloma venereum, attach to the same receptor on the host-cell membrane... - Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immunoblottingL Bini
Department of Molecular Biology, Siena University, Italy
Electrophoresis 17:185-90. 1996..This work provides a preliminary basis for a future and progressive compilation of a genome-linked database of chlamydial proteins... - Diversity among abortion strains of Chlamydia psittaci demonstrated by inclusion morphology, polypeptide profiles and monoclonal antibodiesE Vretou
Department of Biotechnology, Hellenic Pasteur Institute, Athens, Greece
Vet Microbiol 51:275-89. 1996..psittaci serotype 1-subtype. The antigenic diversity among abortion strains should be taken into consideration when designing a subunit vaccine... - Heterogeneity within the first constant segment of the major outer membrane protein gene in Chlamydia trachomatis serovar D/Da distinguishes 2 lineagesC Sayada
Laboratoire de biochemie génétique, INSERM U, 120, Hopital Robert Debre, Paris, France
C R Acad Sci III 318:943-9. 1995..The observed ompl genetic diversity of serovar D/Da strains in regions involved in T- and B-cell responses might add another level of complexity to the design of a subunit vaccine... - The cell nucleus as a possible source of cathodally migrating serum creatine kinaseE Jockers-Wretou
Clin Chem 30:1268-9. 1984 - Serum creatine kinase B subunit levels in neurogenic atrophiesE Jockers-Wretou
J Neurol 232:236-9. 1985..02). It is suggested that the increase of CK-B in neurogenic atrophies is a strong indication of an active regeneration process in the denervated muscle... - Serum creatine kinase MB (heart) isoenzyme activity in schizophreniaE Jockers-Wretou
Biol Psychiatry 20:337-9. 1985 - Characterization of a monoclonal antibody reacting with histone H3S Muller
FEBS Lett 182:459-64. 1985..The monoclonal antibody appears to recognize an epitope situated in the N-terminal residues 20-50 of histone H3; it recognizes this epitope in the octamer aggregate of core histones but not in the core particle... - Structural relationship of the heterogeneous nuclear ribonucleoprotein core polypeptides from rat liver nucleiA Guialis
Arch Biochem Biophys 247:355-64. 1986..Therefore, the core polypeptides cannot be glycoproteins of the general class with affinity for concanavalin A... - Serum creatine kinase B levels in diseases of the central nervous systemD Vassilopoulos
Eur Neurol 27:78-81. 1987..The present findings have to be considered as an indication for further CK isoenzyme studies in systematic disorders of the nervous system... - Autoantibodies to the core proteins of hnRNPsA Dangli
Biological Research Center, National Hellenic Research Foundation, Athens, Greece
FEBS Lett 231:118-24. 1988.... - [Immunohistochemical localization of creatinkinase isoenzymes in human tissue (author's transl)]E Jockers-Wretou
Histochemistry 54:83-95. 1977..The results indicate the usefulness of the combined application of histochemistry and immunotitration in serial tissue sections...