Improved detection of Mycobacterium avium subsp. paratuberculosis In milk by immunomagnetic PCRI R Grant
Department of Food Science Food Microbiology, The Queen s University of Belfast, Newforge Lane, Belfast, UK
Vet Microbiol 77:369-78. 2000
..5% of the same 40 milk samples (sensitivity 23%, specificity 100%). IMS-PCR was also shown to be capable of detecting natural M. ptb infection in raw sheep's milk, and raw and commercially pasteurised cows' milk...
- Incidence of Mycobacterium paratuberculosis in raw sheep and goats' milk in England, Wales and Northern Ireland
I R Grant
Department of Food Science Microbiology, The Queen s University of Belfast, Newforge Lane, Belfast BT9 5PX, UK
Vet Microbiol 79:123-31. 2001
..paratuberculosis were isolated by culture. The results of this study suggest that bulk raw sheep and goats' milk from these regions of the UK may not represent significant vehicles of transmission of M. paratuberculosis to humans...
- Effect of higher pasteurization temperatures, and longer holding times at 72 degrees C, on the inactivation of Mycobacterium paratuberculosis in milk
I R Grant
Department of Food Science Food Microbiology, Queen s University of Belfast, UK
Lett Appl Microbiol 28:461-5. 1999
..It was concluded that a longer holding time is more likely to achieve the complete inactivation of Myco. paratuberculosis in milk than a higher pasteurization temperature...
- Development of an IMS-PCR assay for the detection of Mycobacterium avium ssp. paratuberculosis in water
L Whan
Food Microbiology, The Queen's University of Belfast, Belfast, UK
Lett Appl Microbiol 40:269-73. 2005
..SIGNIFICANCE AND IMPACT OF THE STUDY: A sensitive method for the detection of Map in water involving addition of 0.4% Tween 80, centrifugation and IMS-PCR was developed...
- Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation
I R Grant
Department of Food Science Food Microbiology, The Queen s University of Belfast, Belfast BT9 5PX, United Kingdom
Appl Environ Microbiol 64:3153-8. 1998
..The potential value of our IMS method is as an aid for rapid detection of M. paratuberculosis in milk when it is used in conjunction with end point detection methods, such as IS900 PCR or an enzyme-linked immunosorbent assay...
- Comparative evaluation of four decontamination protocols for the isolation of Mycobacterium avium subsp. paratuberculosis from milk
L Dundee
Department of Food Science (Food Microbiology, Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
Lett Appl Microbiol 33:173-7. 2001
..The "best" decontamination protocol only recovered a fraction of the M.a. paratuberculosis cells present in a milk sample...
- Effect of chemical decontamination and refrigerated storage on the isolation of Mycobacterium avium subsp. paratuberculosis from heat-treated milk
I R Grant
Department of Food Science Food Microbiology, Queen s University of Belfast, Belfast, UK
Lett Appl Microbiol 38:283-8. 2004
..To assess the impact of chemical decontamination and refrigerated storage before culture on the recovery of Mycobacterium avium subsp. paratuberculosis from heat-treated milk...
- Effect of high-temperature, short-time (HTST) pasteurization on milk containing low numbers of Mycobacterium paratuberculosis
I R Grant
Department of Food Science Food Microbiology, Queen s University of Belfast, Northern Ireland, UK
Lett Appl Microbiol 26:166-70. 1998
..IS900-based PCR confirmed that these acid-fast survivors were Myco. paratuberculosis. No viable Myco. paratuberculosis were isolated from HTST-pasteurized milk initially containing either 10 cfu ml-1 or 10 cfu 50 ml-1...
- Zoonotic potential of Mycobacterium avium ssp. paratuberculosis: the current position
I R Grant
Department of Food Microbiology, Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
J Appl Microbiol 98:1282-93. 2005
- Mycobacterium paratuberculosis and milk
I R Grant
Department of Food Science (Microbiology, Queen's University, Belfast, N. Ireland, UK
Acta Vet Scand 44:261-6. 2003
..Hence, both raw and pasteurised cows' milk are potential vehicles of transmission of M. paratuberculosis to humans...
- Bactericidal effect of chlorine on Mycobacterium paratuberculosis in drinking water
L B Whan
Department of Food Science (Food Microbiology, Queen's University Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK
Lett Appl Microbiol 33:227-31. 2001
..SIGNIFICANCE AND IMPACT OF THE STUDY: This work highlights the need for further research into the survival of M. paratuberculosis during water treatment...
- Comparative evaluation of the MGIT and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk
I R Grant
Department of Food Microbiology, Queen s University of Belfast, Belfast, UK
J Appl Microbiol 95:196-201. 2003
..Chemical decontamination before culture caused a significant reduction in numbers of viable M. paratuberculosis in all spiked milk samples resulting in decreased detection capability for both culture systems...
- Mycobacterium avium ssp. paratuberculosis and its potential survival tactics
M T Rowe
Food Microbiology Branch, Agriculture, Food and Environmental Science Division, Department of Agriculture and Rural Development for Northern Ireland, Belfast, UK
Lett Appl Microbiol 42:305-11. 2006
..It then focuses on the potential mechanisms Map might employ to survive and disseminate in the environment, including interaction with protozoa and insects, dormancy, biofilm formation and aerosolization...
