Toxicity testing of a novel riboflavin-based technology for pathogen reduction and white blood cell inactivationHeather L Reddy
Navigant Biotechnologies, Inc, Lakewood, CO, USA
Transfus Med Rev 22:133-53. 2008
..The possible presence of leachable or extractable compounds (from the disposable set) was evaluated with novel assays for measuring these compounds in blood. No treatment-related toxicity was observed in any of the studies...
- Evaluation of potential immune response and in vivo survival of riboflavin-ultraviolet light-treated red blood cells in baboons
Raymond P Goodrich
Navigant Biotechnologies, Lakewood, Colorado 80227, USA
Transfusion 49:64-74. 2009
..This study evaluated the hypothesis that there is no immune response to riboflavin-and-ultraviolet [UV]-light-treated red blood cells (RBCs), as observed by serology and by survival of RBCs in circulation...
- The Mirasol PRT system for pathogen reduction of platelets and plasma: an overview of current status and future trends
Raymond P Goodrich
Navigant Biotechnologies Inc, Lakewood, CO 80215, USA
Transfus Apher Sci 35:5-17. 2006
..The Mirasol PRT process is based on riboflavin photochemistry. This manuscript reviews current progress and future trends...
- Correlation of in vitro platelet quality measurements with in vivo platelet viability in human subjects
Raymond P Goodrich
Navigant Biotechnologies, Inc, Lakewood, CO 80215, USA
Vox Sang 90:279-85. 2006
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- Design and development of a method for the reduction of infectious pathogen load and inactivation of white blood cells in whole blood products
Raymond P Goodrich
CaridianBCT Biotechnologies LLC, 1215 Quail Street, Lakewood, CO 80215, USA
Biologicals 38:20-30. 2010
..This manuscript describes development efforts using a photochemical PRT method employing riboflavin and UV-Light (Mirasol PRT)...
- Platelet glycolytic flux increases stimulated by ultraviolet-induced stress is not the direct cause of platelet morphology and activation changes: possible implications for the role of glucose in platelet storage
Junzhi Li
Navigant Biotechnologies, Inc, Lakewood, CO 80215, USA
Transfusion 45:1750-8. 2005
..CONCLUSIONS: Increased glycolytic flux is not a direct cause for PLT morphology change and spontaneous activation during storage lesion development. Reduction of glucose utilization may increase PLT loss during storage...
- Evaluation of platelet mitochondria integrity after treatment with Mirasol pathogen reduction technology
Junzhi Li
Navigant Biotechnologies, Inc, Lakewood, Colorado 80215, USA
Transfusion 45:920-6. 2005
..An increased demand for ATP may be the driving force for observed increases in both the glycolytic flux and the oxidative metabolism observed in treated PLTs...
- Protein stability of previously frozen plasma, riboflavin and UV light-treated, refrozen and stored for up to 2 years at -30 °C
Anna Ettinger
CaridianBCT Biotechnologies, LLC, Lakewood, CO 80215, United States
Transfus Apher Sci 44:25-31. 2011
..The objective of this study was to evaluate the in vitro protein quality of previously frozen FFP, thawed, Mirasol-treated and refrozen for a final storage period of up to 2 years at -30 °C...
- A laboratory comparison of pathogen reduction technology treatment and culture of platelet products for addressing bacterial contamination concerns
Raymond P Goodrich
CaridianBCT Biotechnologies LLC, Lakewood, CO 80215, USA
Transfusion 49:1205-16. 2009
..Concerns over the risk of bacterial contamination of platelet products have led to implementation of bacteria culture and other screening methods. New approaches for dealing with this issue have also been proposed...
- Photochemical inactivation of selected viruses and bacteria in platelet concentrates using riboflavin and light
Patrick H Ruane
Navigant Biotechnologies, Lakewood, Colorado 80215, USA
Transfusion 44:877-85. 2004
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- Quality of proteins in riboflavin and UV light-treated FFP during 1 year of storage at -18°C
Anna Ettinger
CaridianBCT Biotechnologies, LLC, Lakewood, CO 80215, USA
Transfus Apher Sci 46:15-8. 2012
..Comparisons were made to paired, untreated controls after 6 months and 1 year of storage...
- Riboflavin and UV-light based pathogen reduction: extent and consequence of DNA damage at the molecular level
Vijay Kumar
Navigant Biotechnologies, 1215 Quail Street, Lakewood, CO 80215, USA
Photochem Photobiol 80:15-21. 2004
..These data also suggest that the type and extent of damage to DNA for virus in the presence of RB and light make it less likely to be repaired by normal repair pathways available in host cells...
- Preparation of cryoprecipitate from riboflavin and UV light-treated plasma
Anna Ettinger
CaridianBCT Biotechnologies, LLC, Lakewood, CO, USA
Transfus Apher Sci 46:153-8. 2012
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- White blood cell inactivation after treatment with riboflavin and ultraviolet light
Susanne Marschner
CaridianBCT Biotechnologies, LLC, Lakewood, Colorado 80215, USA
Transfusion 50:2489-98. 2010
..Data from in vitro studies and this clinical trial suggest that PRT treatment may be as effective as gamma irradiation in preventing TA-GVHD and more effective than leukoreduction in preventing alloimmunization...
- Separation, identification and quantification of riboflavin and its photoproducts in blood products using high-performance liquid chromatography with fluorescence detection: a method to support pathogen reduction technology
Christopher C Hardwick
Navigant Biotechnologies Inc, Lakewood, CO 80215, USA
Photochem Photobiol 80:609-15. 2004
..Additional photoproducts could also be identified and quantified using this method. The presence of these agents in normal, untreated blood suggests that their presence in blood is ubiquitous...
- Defining "adequate" pathogen reduction performance for transfused blood components
Raymond P Goodrich
CaridianBCT Biotechnologies, LLC, Lakewood, Colorado 80215, USA
Transfusion 50:1827-37. 2010
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- Efficacy of apheresis platelets treated with riboflavin and ultraviolet light for pathogen reduction
James P AuBuchon
Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA
Transfusion 45:1335-41. 2005
..The recovery, survival, and metabolic properties of Mirasol PLTs should provide sufficient hemostatic support in thrombocytopenia to justify patient clinical trials...
- Treatment with riboflavin and ultraviolet light prevents alloimmunization to platelet transfusions and cardiac transplants
Hiroshi Asano
Department of Pathology, John Hopkins Medical Institutions, Baltimore, MD 21205 2196, USA
Transplantation 84:1174-82. 2007
..The current study evaluated the ability of Mirasol treatment to prevent alloimmunization by platelet transfusions in rats...
- Comparison of computerized formulae for determination of platelet recovery and survival
James P AuBuchon
Transfusion 45:1237-9. 2005
