Research Topics
Genomes and Genes | P H SeeburgSummaryAffiliation: University of Heidelberg Country: Germany Publications
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Publications
RED2, a brain-specific member of the RNA-specific adenosine deaminase familyT Melcher
Laboratory for Neurobiology, Max Planck Institute for Medical Research, Jahnstrasse 12, 69120 Heidelberg, Germany
J Biol Chem 271:31795-8. 1996..However, a chimera of RED1 and RED2 edited the GluR-B Q/R and R/G sites with moderate efficiency. Our data suggest that RED2 may edit brain-specific transcripts with distinct structural features...- Genetic manipulation of key determinants of ion flow in glutamate receptor channels in the mouseP H Seeburg
Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Jahnstr 29, 69120, Heidelberg, Germany
Brain Res 907:233-43. 2001..Therefore, the impact on adult synaptic function and plasticity by glutamate receptor channels with changed ion selectivity and conductance needs to be addressed by conditional expression of the mutant receptors... - Conditional restoration of hippocampal synaptic potentiation in Glur-A-deficient miceV Mack
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, D 69120 Heidelberg, Germany
Science 292:2501-4. 2001..This shows that GluR-A-independent forms of synaptic plasticity can mediate the establishment of mature hippocampal circuits that are prebuilt to express GluR-A-dependent LTP... - Point mutation in an AMPA receptor gene rescues lethality in mice deficient in the RNA-editing enzyme ADAR2M Higuchi
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Heidelberg, Germany
Nature 406:78-81. 2000..We conclude that this transcript is the physiologically most important substrate of ADAR2... - Importance of AMPA receptors for hippocampal synaptic plasticity but not for spatial learningD Zamanillo
Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Science 284:1805-11. 1999..The results suggest that CA1 hippocampal LTP is controlled by the number or subunit composition of AMPA receptors and show a dichotomy between LTP in CA1 and acquisition of spatial memory... - Channel-lining residues of the AMPA receptor M2 segment: structural environment of the Q/R site and identification of the selectivity filterT Kuner
Abteilung Molekulare Neurobiologie, Abteilung Zellphysiologie, Max Planck Institut fur medizinische Forschung, 69120 Heidelberg, Germany
J Neurosci 21:4162-72. 2001.... - Dysfunctions in mice by NMDA receptor point mutations NR1(N598Q) and NR1(N598R)F N Single
Max Planck Institute for Medical Research, Departments of Molecular Neuroscience and Cell Physiology, Jahnstrabetae 29, D 69120 Heidelberg, Germany
J Neurosci 20:2558-66. 2000..We conclude that disturbed NMDA receptor signaling mediates a variety of neurological phenotypes... - Neurological dysfunctions in mice expressing different levels of the Q/R site-unedited AMPAR subunit GluR-BD Feldmeyer
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Heidelberg, Germany
Nat Neurosci 2:57-64. 1999..Thus, changes in GluR-B gene expression and Q/R site editing can affect critical architectural and functional aspects of excitatory principal neurons... - Candidate editases for GluR channels in single neurons of rat hippocampus and cerebellumG Kohr
Max Planck Institute for Medical Research, Molecular Neurobiology, Heidelberg, Germany
Neuropharmacology 37:1411-7. 1998..The differential ADAR expression revealed by our study might underlie the distinct editing efficiencies and selectivities in different GluR subunit transcripts... - Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studiesD J Spergel
Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120, Heidelberg, Germany
Prog Neurobiol 63:673-86. 2001.... - Rapid construction in yeast of complex targeting vectors for gene manipulation in the mouseT Storck
Center for Molecular Biology ZMBH, University of Heidelberg, Germany
Nucleic Acids Res 24:4594-6. 1996..In this way, complex targeting vectors can be engineered with considerable ease and speed, obviating extensive gene mapping in search for suitable restriction sites... - Codon-improved Cre recombinase (iCre) expression in the mouseD R Shimshek
Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Heidelberg, Germany
Genesis 32:19-26. 2002..Thus, whereas efficient activation of Cre fusion proteins in the brain needs further improvements, our studies indicate that iCre should facilitate genetic experiments in the mouse... - The AMPA receptor subunit GluR-B in its Q/R site-unedited form is not essential for brain development and functionK Kask
Department of Molecular Neuroscience, Max Planck Institute for Medical Research, Jahnstrasse 29, 69120 Heidelberg, Germany
Proc Natl Acad Sci U S A 95:13777-82. 1998..This constitutes proof in vivo that elevated calcium influx through AMPARs need not generate pathophysiological consequences... - Hypertrophy and altered activity of the adrenal cortex in Homer 1 knockout miceV Grinevich
Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Jahnstraße 29, 69120 Heidelberg, Germany
Horm Metab Res 43:551-6. 2011..These results, together with the detection of Homer 1 mRNA and protein in the adrenal cortex of WT mice, indicate that Homer 1 directly affects the steroidogenic function of the adrenal glands... - RNA editing of brain glutamate receptor channels: mechanism and physiologyP H Seeburg
Max Planck Institute for Medical Research, Department of Molecular Neuroscience, Jahnstr 29, 69120 Heidelberg, Germany
Brain Res Brain Res Rev 26:217-29. 1998..If so, RNA editing by adenosine deamination may significantly enlarge the functional repertoire of the mammalian genome... - Cloning of a human RNA editing deaminase (ADARB1) of glutamate receptors that maps to chromosome 21q22.3L Mittaz
Department of Genetics and Microbiology, University of Geneva Medical School, Switzerland
Genomics 41:210-7. 1997.... - EphrinB ligands recruit GRIP family PDZ adaptor proteins into raft membrane microdomainsK Brückner
Developmental Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany
Neuron 22:511-24. 1999..Our findings suggest that GRIP proteins provide a scaffold for the assembly of a multiprotein signaling complex downstream of ephrinB ligands... - The TiPS/TINS lecture: the molecular biology of mammalian glutamate receptor channelsP H Seeburg
University of Heidelberg, Centre of Molecular Biology ZMBH, Germany
Trends Pharmacol Sci 14:297-303. 1993..This review describes the different channel types obtained by recombinant means and the genetic mechanisms controlling the expression of functionally important channel structures... - Early-onset epilepsy and postnatal lethality associated with an editing-deficient GluR-B allele in miceR Brusa
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany
Science 270:1677-80. 1995..These mice developed seizures and died by 3 weeks of age, showing that GluR-B pre-mRNA editing is essential for brain function... - The human growth hormone gene family: nucleotide sequences show recent divergence and predict a new polypeptide hormoneP H Seeburg
DNA 1:239-49. 1982..The regions downstream of the polyadenylation sites in the genes for hGH and its variant, but not for hCS, contain members of an Alu family repeat sequence... - Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedureA Ullrich
DNA 3:387-92. 1984..Regions of homology and variability within the kallikrein gene family are maintained in this protein and probably reflect the similar functions and different substrates for this family of proteins... - Expression of active human factor VIII from recombinant DNA clonesW I Wood
Nature 312:330-7. 1984..The recombinant protein corrects the clotting time of plasma from haemophiliacs and has many of the biochemical and immunological characteristics of serum-derived factor VIII... - Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxinD Pennica
Nature 312:724-9. 1984..Recombinant tumour necrosis factor can be obtained by expression of its complementary DNA in Escherichia coli and induces the haemorrhagic necrosis of transplanted methylcholanthrene-induced sarcomas in syngeneic mice... - In vitro deletional mutagenesis for bacterial production of the 20,000-dalton form of human pituitary growth hormoneJ P Adelman
DNA 2:183-93. 1983..A gene with the correct deletion was used to express the short hGH variant in E. coli... - Cloning and expression of human tissue-type plasminogen activator cDNA in E. coliD Pennica
Nature 301:214-21. 1983 - The organization of the gene for the functionally dominant alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid receptor subunit GluR-BM Kohler
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Federal Republic of Germany
J Biol Chem 269:17367-70. 1994..The major GluR-B mRNAs in murine brain, 4 and 6 kilobase differ in the length of their 3'-untranslated region... - Benzodiazepine-induced motor impairment linked to point mutation in cerebellar GABAA receptorE R Korpi
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany
Nature 361:356-9. 1993..Our results suggest that cerebellar motor control may be a distinct behavioural correlate of the alpha 6-subunit-containing GABAA receptor subtype... - Gene structure of the murine N-methyl D-aspartate receptor subunit NR2CB Suchanek
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Federal Republic of Germany
J Biol Chem 270:41-4. 1995..Comparison of intron positions in genes encoding different members of the glutamate receptor family confirms a close evolutionary relationship of the NR2C and NMDAR1 subunit genes... - Prominent dendritic localization in forebrain neurons of a novel mRNA and its product, dendrinA Herb
Department of Molecular Neurobiology, Max Planck Institute for Medical Research and Laboratory for Molecular Neuroendocrinology, Heidelberg, Germany
Mol Cell Neurosci 8:367-74. 1997..The restricted expression and dendritic location are compatible with a role for DEN in synaptic plasticity of central neocortical forebrain neurons... - The GABAA receptor family: molecular and functional diversityP H Seeburg
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Federal Republic of Germany
Cold Spring Harb Symp Quant Biol 55:29-40. 1990 - Impaired spatial working memory but spared spatial reference memory following functional loss of NMDA receptors in the dentate gyrusB Niewoehner
Department of Experimental Psychology, University of Oxford, South Parks Road, Oxford OX1 3UD, UK
Eur J Neurosci 25:837-46. 2007.... - A mammalian RNA editing enzymeT Melcher
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Germany
Nature 379:460-4. 1996..Both deaminases edit the R/G site in GluR-B pre-mRNA, indicating that members of an emerging gene family catalyse adenosine deamination in nuclear transcripts with distinct but overlapping substrate specificities... - The rat delta-1 and delta-2 subunits extend the excitatory amino acid receptor familyH Lomeli
Laboratory of Molecular Neuroscience, Center for Molecular Biology ZMBH, Heidelberg University, Germany
FEBS Lett 315:318-22. 1993..However, delta-1 gene expression undergoes a pronounced developmental peak, with particularly high mRNA levels in the caudate putamen of late embryonic/early postnatal stages... - Differential signal transduction by five splice variants of the PACAP receptorD Spengler
CNRS UPR 9023, Mecanismes Moleculaires des Communications Cellulaires, CCIPE, Montpellier, France
Nature 365:170-5. 1993..Expression of these cDNAs revealed altered patterns of adenylyl cyclase and phospholipase C stimulation, suggesting a novel mechanism for fine tuning of signal transduction... - RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiencyM Higuchi
Center for Molecular Biology, University of Heidelberg, Federal Republic of Germany
Cell 75:1361-70. 1993..Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA... - Cloning and characterization of the rat 5-HT5B receptor. Evidence that the 5-HT5B receptor couples to a G protein in mammalian cell membranesW Wisden
Center for Molecular Biology, University of Heidelberg ZMBH, Germany
FEBS Lett 333:25-31. 1993..In the rat brain, 5-HT5B gene expression occurs predominantly in the medial habenulae and hippocampal CA1 cells of the adult. Little expression is seen during embryonic development... - The c-sis gene encodes a precursor of the B chain of platelet-derived growth factorA Johnsson
EMBO J 3:921-8. 1984..This chain is 60% homologous to the B chain and cannot be encoded by that part of c-sis which has been sequenced but could be encoded by sequences which lie 5' to the five regions of v-sis homology in c-sis, or at a separate locus... - Structure and organization of the human Ki-ras proto-oncogene and a related processed pseudogeneJ P McGrath
Nature 304:501-6. 1983..This suggests that the functional gene may specify either of two related polypeptides depending on the pattern of RNA splicing... - Saccharomyces cerevisiae contains two discrete genes coding for the alpha-factor pheromoneA Singh
Nucleic Acids Res 11:4049-63. 1983..Thus, the alpha-factor can be derived from 2 different precursor proteins of 165 and 120 amino acids containing, respectively, 4 and 2 copies of the pheromone... - Molecular cloning and expression of cDNA encoding a peripheral-type benzodiazepine receptorR Sprengel
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, Federal Republic of Germany
J Biol Chem 264:20415-21. 1989..These studies suggest that PKBS comprises binding domains for benzodiazepines and isoquinoline carboxamides and hence is apparently responsible for the manifestation of peripheral-type benzodiazepine recognition sites... - GABAA receptor beta subunit heterogeneity: functional expression of cloned cDNAsS Ymer
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG
EMBO J 8:1665-70. 1989..These results, together with the known alpha subunit heterogeneity, suggest that a variety of related but functionally distinct GABAA receptor subtypes are generated by different subunit combinations... - The dopamine D2 receptor: two molecular forms generated by alternative splicingR Dal Toso
Laboratory of Molecular Neuroendocrinology, ZMBH, Heidelberg, FRG
EMBO J 8:4025-34. 1989..Both receptors expressed in cultured mammalian cells bind [3H]spiperone with high affinity and inhibit adenylyl cyclase, as expected of the D2 receptor subtype... - Lutropin-choriogonadotropin receptor: an unusual member of the G protein-coupled receptor familyK C McFarland
Department of Developmental Biology, Genetech, Inc, South San Francisco, CA 94080
Science 245:494-9. 1989..As revealed by RNA blot analysis and in situ hybridization, the 4.4-kilobase cognate messenger RNA is prominently localized in the rat ovary... - Sequence and expression of human GABAA receptor alpha 1 and beta 1 subunitsP R Schofield
Zentrum fur Molekulare Biologie, University of Heidelberg, FRG
FEBS Lett 244:361-4. 1989..The cloned human GABAA receptor subunits induce the formation of GABA-gated chloride channels when expressed in mammalian cells... - The murine GABAA receptor delta-subunit gene: structure and assignment to human chromosome 1B Sommer
Laboratory of Molecular Neuroendocrinology, ZMBH, University of Heidelberg, FRG
DNA Cell Biol 9:561-8. 1990..The human delta subunit gene was localized on the short arm of chromosome 1... - Structural and functional characterization of the gamma 1 subunit of GABAA/benzodiazepine receptorsS Ymer
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG
EMBO J 9:3261-7. 1990..g. flumazenil) and negative modulators (e.g. DMCM). These findings reveal a pronounced effect of gamma subunit variants on GABAA/benzodiazepine receptor pharmacology... - Alpha subunit variants of the human glycine receptor: primary structures, functional expression and chromosomal localization of the corresponding genesG Grenningloh
ZMBH, University of Heidelberg, FRG
EMBO J 9:771-6. 1990..2-p22.1) of the human X chromosome. In contrast, the alpha 1 subunit gene is autosomally located. These data indicate molecular heterogeneity of the human GlyR at the level of alpha subunit genes... - Molecular cloning and characterization of a rat brain cDNA encoding a 5-hydroxytryptamine1B receptorM M Voigt
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG
EMBO J 10:4017-23. 1991..mRNA expression was also detected in cells within the CA1 region of hippocampus, striatum, layer 4 of cortex and in the cerebellum, suggesting a previously unrecognized post-synaptic role for the 5-HT1B receptor... - RNA editing in brain controls a determinant of ion flow in glutamate-gated channelsB Sommer
Laboratory for Molecular Neuroendocrinology, University of Heidelberg, Federal Republic of Germany
Cell 67:11-9. 1991..This process apparently edits subunit transcripts of the two glutamate receptor classes with different efficiency and selectivity... - Functional and molecular distinction between recombinant rat GABAA receptor subtypes by Zn2+A Draguhn
Max Planck Institut für medizinische Forschung Abteilung Zellphysiologie, Heidelberg, Federal Republic of Germany
Neuron 5:781-8. 1990.... - Cloning, pharmacological characteristics and expression pattern of the rat GABAA receptor alpha 4 subunitW Wisden
Laboratory Molecular Neuroendocrinology, Center for Molecular Biology, Heidelberg, Germany
FEBS Lett 289:227-30. 1991..The alpha 4 mRNA is found at lower levels in cortex and caudate putamen, and is rare in cerebellum... - Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cellsP Werner
Laboratory of Molecular Neuroendocrinology, Centre for Molecular Biology, University of Heidelberg, Germany
Nature 351:742-4. 1991.... - The KA-2 subunit of excitatory amino acid receptors shows widespread expression in brain and forms ion channels with distantly related subunitsA Herb
Center for Molecular Biology, Heidelberg University, Germany
Neuron 8:775-85. 1992..GluR6/KA-2 channels are gated by AMPA, which fails to gate homomeric GluR6 receptor channels. These results suggest possible in vivo partnership relations for high affinity kainate receptors... - A glutamate receptor channel with high affinity for domoate and kainateB Sommer
Laboratory of Molecular Neuroendocrinology, Heidelberg University, FRG
EMBO J 11:1651-6. 1992..These findings suggest the existence of at least two distinct types of non-NMDA receptor channels, both gated by AMPA and kainate, but differing in pharmacology and current properties... - Heteromeric NMDA receptors: molecular and functional distinction of subtypesH Monyer
Center for Molecular Biology, University of Heidelberg, Germany
Science 256:1217-21. 1992..Such heteromeric NMDA receptor subtypes may exist in neurons, since NR1 messenger RNA is synthesized throughout the mature rat brain, while NR2 messenger RNA show a differential distribution... - Sequence and expression of a novel GABAA receptor alpha subunitS Ymer
Laboratory of Molecular Neuroendocrinology, Center for Molecular Biology ZMBH, Heidelberg, FRG
FEBS Lett 258:119-22. 1989..Northern analysis indicates that a 4 kb alpha 4 mRNA is expressed in the calf cerebellum, cortex and hippocampus but is barely detectable in the rat brain... - Two novel GABAA receptor subunits exist in distinct neuronal subpopulationsB D Shivers
Center for Molecular Biology, Federal Republic of Germany
Neuron 3:327-37. 1989..These findings have implications for the composition of two different GABAA receptor subtypes and for information processing in networks using GABA for signaling... - The human growth hormone gene family: structure and evolution of the chromosomal locusG S Barsh
Nucleic Acids Res 11:3939-58. 1983.... - Efficient bacterial expression of bovine and porcine growth hormonesP H Seeburg
DNA 2:37-45. 1983..The cloned cDNAs were modified using synthetic DNA to construct expression vectors for efficient bacterial production of the mature animal growth hormones... - The human pro-opiomelanocortin gene: organization, sequence, and interspersion with repetitive DNAP L Whitfeld
DNA 1:133-43. 1982..The very high degree of homology in the amino terminal portion of POMC is consistent with an important physiological role for this peptide... - Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologueD J Capon
Nature 302:33-7. 1983.... - The sequence of human serum albumin cDNA and its expression in E. coliR M Lawn
Nucleic Acids Res 9:6103-114. 1981..The DNA sequence predicts a mature protein of 585 amino acids preceded by a 24 amino acid "prepro" peptide... - The structure of eight distinct cloned human leukocyte interferon cDNAsD V Goeddel
Nature 290:20-6. 1981..The nucleotide sequences demonstrate that the multiple human LeIFN genes code for a family of homologous, yet distinct proteins. One of the cDNA clones may have been derived from the transcription of a LeIFN pseudogene... - Human leukocyte interferon produced by E. coli is biologically activeD V Goeddel
Nature 287:411-6. 1980..An expression plasmid was constructed which permits the synthesis in E. coli of 2.5 x 10(8) units of interferon per litre of culture. This LeIF protected squirrel monkeys from lethal encephalomyocarditis virus infection... - Characterization of cDNA for precursor of human luteinizing hormone releasing hormoneP H Seeburg
Nature 311:666-8. 1984.... - Structure of two human ovarian inhibinsA J Mason
Biochem Biophys Res Commun 135:957-64. 1986.... - Molecular cloning and amino acid sequence of rat enkephalinaseB Malfroy
Biochem Biophys Res Commun 144:59-66. 1987..Enkephalinase, a zinc-containing metallo enzyme, displays homology with other zinc metallo enzymes such as carboxypeptidase A, B and E, suggesting enzymatic similarities in these enzymes... - Molecular cloning and amino acid sequence of human enkephalinase (neutral endopeptidase)B Malfroy
Department of Pharmacological Sciences, Genentech, Inc, South San Francisco, CA 94080
FEBS Lett 229:206-10. 1988.... - The human growth hormone gene locus: structure, evolution, and allelic variationsH Hirt
DNA 6:59-70. 1987..The structure of the hCS genes is discussed in light of recent studies of CS genes from other mammalian species... - A deletion truncating the gonadotropin-releasing hormone gene is responsible for hypogonadism in the hpg mouseA J Mason
Science 234:1366-71. 1986.... - Cloning and expression of cDNA for human diazepam binding inhibitor, a natural ligand of an allosteric regulatory site of the gamma-aminobutyric acid type A receptorP W Gray
Proc Natl Acad Sci U S A 83:7547-51. 1986..The role of these sites and DBI in adrenal gland, testis, and kidney remains to be determined... - A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormoneK Nikolics
Nature 316:511-7. 1985..Active immunization with peptides corresponding to GAP sequences led to greatly increased prolactin secretion in rabbits... - Structure and functional expression of cloned rat serotonin 5HT-2 receptorD B Pritchett
Laboratory of Molecular Neuroendocrinology, University of Heidelberg, FRG
EMBO J 7:4135-40. 1988..The agonist also elicited a current flow, blocked by spiperone, in Xenopus oocytes injected with in vitro synthesized RNA containing the cloned nucleotide sequences... - The third gamma subunit of the gamma-aminobutyric acid type A receptor familyA Herb
Laboratory for Molecular Neuroendocrinology, Zentrum fur Molekulare Biologie, ZMBH, Heidelberg, Germany
Proc Natl Acad Sci U S A 89:1433-7. 1992..Furthermore, the presence of gamma 3 in place of gamma 2 in alpha 1 beta 2 gamma x receptors generates a marked decrease in the affinity of agonists while leaving the affinity of antagonists or negative modulators largely unaffected...