Research Topics
| Thomas MothesSummaryAffiliation: University of Leipzig Country: Germany Publications
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Detail Information
Publications
How gluten-free is gluten-free, and what does this mean to coeliac patients?Thomas Mothes
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital of Leipzig, Germany
Eur J Gastroenterol Hepatol 15:461-3. 2003..Further clinical studies using small dose challenges and dietary records including gluten analysis are necessary until a more meaningful discussion on standards for gluten-free foods can be started...- [Recent aspects of antibody determination for the diagnosis of coeliac disease]T Mothes
Institut für Laboratoriumsmedizin, Klinische Chemie und Molekulare Diagnostik des Universitätsklinikums Leipzig
Dtsch Med Wochenschr 134:1525-8. 2009..Prospective studies are needed to show whether antibody assays could replace biopsy in the diagnosis of coeliac disease in a substantial number of patients... - New developments in serodiagnosis of childhood celiac disease: assay of antibodies against deamidated gliadinChristian Prause
Institute of Laboratory Medicine, University Hospital Leipzig, Leipzig, Germany
Ann N Y Acad Sci 1173:28-35. 2009..The new IgG-anti-GAF3X ELISA was also able to detect CD in three cases of IgA deficiency and in two cases of latent CD and was also useful in the diagnosis of children younger than 2 years of age... - Serologic assay based on gliadin-related nonapeptides as a highly sensitive and specific diagnostic aid in celiac diseaseElke Schwertz
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, Institute of Clinical Immunology, University Hospital, Leipzig, Germany
Clin Chem 50:2370-5. 2004..007), and the AtTGAs correctly classified 98%. CONCLUSION: The peptide antibody assay has higher diagnostic accuracy than AGAs for distinguishing patients with CD from controls, and has diagnostic accuracy similar to that of AtTGAs... - Quantification of human tissue transglutaminase by a luminescence sandwich enzyme-linked immunosorbent assayJohannes Wolf
Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics, University Hospital Leipzig, D 04103 Leipzig, Germany
Anal Biochem 419:153-60. 2011..The new sandwich ELISA may be successfully applied for investigation of physiological functions of tTG and of disorders associated with inadequate tTG expression... - Antibodies against deamidated gliadin as new and accurate biomarkers of childhood coeliac diseaseChristian Prause
Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, Germany
J Pediatr Gastroenterol Nutr 49:52-8. 2009..Assays of antibodies against native gliadin (anti-nGli) lost importance due to low validity. We investigated the performance of new assays for antibodies against deamidated gliadin (anti-dGli) in childhood CD... - Production of recombinant human tissue transglutaminase using the baculovirus expression system, and its application for serological diagnosis of coeliac diseaseAwad A Osman
Institute of Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital, Leipzig, Germany
Eur J Gastroenterol Hepatol 14:1217-23. 2002..However, there were eight patients positive for anti-hu-tTG but negative for anti-gp-tTG. CONCLUSIONS: The new test reaches and even exceeds diagnostic efficiency of EmA for coeliac diagnosis... - Immunoassay of in vitro activated human tissue transglutaminaseJohannes Wolf
Institute of Laboratory Medicine, University Hospital Leipzig, D 04103 Leipzig, Germany
Anal Biochem 411:10-5. 2011..The new assay represents a promising tool for the study of tTG in normal cell physiology and under pathological conditions... - Assay of gliadin by real-time immunopolymerase chain reactionNadine Henterich
, Klinische Chemie und Molekulare Diagnostik, , Leipzig, Germany
Nahrung 47:345-8. 2003..Furthermore, this is the first approach to perform real-time iPCR in one step without changing the reaction vessels after enzyme immunoassay for subsequent PCR analysis thus minimizing risks of contamination and loss of sensitivity... - Specificity analysis of anti-gliadin mouse monoclonal antibodies used for detection of gliadin in food for gluten-free dietDaniel Sanchez
Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Prague 4, 142 20 Czech Republic
J Agric Food Chem 55:2627-32. 2007..The comparison of data obtained using the newly developed ELISA kit and commercially available ones indicated that Abs selection on the basis of their fine specificity to gliadin is useful for sensitive detection of gliadin in foods... - Deamidated gliadin peptides as targets for celiac disease-specific antibodiesThomas Mothes
Institute for Laboratory Medicine, Clinical Chemistry, and Molecular Diagnostics, University Hospital and Medical Faculty of the University, Leipzig, Germany
Adv Clin Chem 44:35-63. 2007.... - Epitopes of calreticulin recognised by IgA autoantibodies from patients with hepatic and coeliac diseaseDaniel Sanchez
Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, 142204 Prague, Czech Republic
J Autoimmun 21:383-92. 2003..Identification of disease specific CRT epitopes contributes to clarification of autoreactivity against this molecule...