Research Topics
Genomes and Genes | T KriegSummaryAffiliation: University of Cologne Country: Germany Publications
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Publications
High frequency of corticosteroid and immunosuppressive therapy in patients with systemic sclerosis despite limited evidence for efficacyNicolas Hunzelmann
Department of Dermatology and Venerology, University of Cologne, Cologne, Germany
Arthritis Res Ther 11:R30. 2009..The objective of this study was to determine the extent to which SSc patients are treated with corticosteroids and immunosuppressive agents...
Skin disease: a cardinal feature of systemic sclerosisT Krieg
Department of Dermatology, University of Cologne, Cologne, Germany
Rheumatology (Oxford) 48:iii14-8. 2009..These include the intravenous prostanoid iloprost and the dual endothelin receptor antagonist bosentan, which is approved in Europe for the prevention of new digital ulcers...
The extracellular matrix of the dermis: flexible structures with dynamic functionsThomas Krieg
Department of Dermatology, Medical Faculty, University of Cologne, Cologne, Germany
Exp Dermatol 20:689-95. 2011..They now permit to design new concepts for the treatment of more common diseases associated with the accumulation of connective tissue and alterations of the biomechanical properties of the extracellular matrix...
Fibrosis in connective tissue disease: the role of the myofibroblast and fibroblast-epithelial cell interactionsThomas Krieg
Department of Dermatology, University of Koln, Kerpener Strasse, D 50924 Koln, Germany
Arthritis Res Ther 9:S4. 2007..The tight skin mouse (Tsk-1) model of cutaneous fibrosis suggests that numerous other genes may also be important...
Cell-matrix interactions in dermal repair and scarringBeate Eckes
Department of Dermatology, University of Cologne, Cologne, Germany
Fibrogenesis Tissue Repair 3:4. 2010..Understanding this complex interplay is important to provide a basis for designing effective wound therapy and for strategic interference with mechanisms that have gone out of control in fibrotic conditions...
Effect of interleukin-10 on the gene expression of type I collagen, fibronectin, and decorin in human skin fibroblasts: differential regulation by transforming growth factor-beta and monocyte chemoattractant protein-1T Yamamoto
Department of Dermatology, University of Cologne, Joseph Stelzmann Strasse 9, Cologne, 50924, Germany
Biochem Biophys Res Commun 281:200-5. 2001..Our data indicate that IL-10 differentially regulates TGF-beta and MCP-1 in the modulation of ECM proteins and therefore suggest that IL-10 plays a role in the regulation of tissue remodeling...
Fibroblasts in mechanically stressed collagen lattices assume a "synthetic" phenotypeD Kessler
Department of Dermatology, Joseph-Stelzmann Strasse 9, University of Cologne, , Germany
J Biol Chem 276:36575-85. 2001....
Contraction-dependent apoptosis of normal dermal fibroblastsS Niland
Department of Dermatology, University of Koln, and Institute of Physiological Chemistry, University of Munster, Germany
J Invest Dermatol 116:686-92. 2001....
Fibroblast expression of collagen integrin receptors alpha1beta1 and alpha2beta1 is not changed in systemic sclerodermaK Herzhoff
Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, D 50924 Cologne, Germany
Br J Dermatol 141:218-23. 1999..These observations indicate that any abnormal response of SSc fibroblasts to their matrix environment is not controlled at the level of receptor expression...
Monocyte chemoattractant protein-1 enhances gene expression and synthesis of matrix metalloproteinase-1 in human fibroblasts by an autocrine IL-1 alpha loopT Yamamoto
Department of Dermatology, University of Cologne, Germany
J Immunol 164:6174-9. 2000..These data demonstrate that MCP-1 up-regulates MMP-1 mRNA expression and synthesis in human skin fibroblasts at a transcriptional level and provide evidence that this is mediated by an IL-1 alpha autocrine loop...
Expression of monocyte chemoattractant protein-1 in the lesional skin of systemic sclerosisT Yamamoto
Department of Dermatology, University of Cologne, Joseph Stelzmann Street 9, 50924, Cologne, Germany
J Dermatol Sci 26:133-9. 2001..These findings suggest that MCP-1 plays a role in recruiting monocyte/macrophages in the lesional skin of scleroderma and that activated fibroblasts in scleroderma are involved in this process...
Regulation of connective tissue homeostasis in the skin by mechanical forcesB Eckes
Department of Dermatology, University of Cologne, Germany
Clin Exp Rheumatol 22:S73-6. 2004..Mechanical tension can therefore be regarded as an additional important regulatory parameter, which we would like to better understand with respect to mechanosensing cellular structures and specific or shared signaling pathways...
Apoptosis in v-myc-transfected MSU-1.1 fibroblasts is induced by cell-matrix contact and differs from that of normal dermal fibroblastsS Niland
Department of Dermatology, University of Cologne, Germany
In Vitro Cell Dev Biol Anim 37:606-12. 2001..1. Further, apoptosis by MSU-1.l fibroblasts was also observed in anchored, i.e., restrained collagen lattices, an environment that supports proliferation of NDF...
Role of stem cell factor and monocyte chemoattractant protein-1 in the interaction between fibroblasts and mast cells in fibrosisT Yamamoto
Department of Dermatology, University of Cologne, Cologne, Germany
J Dermatol Sci 26:106-11. 2001..Our data may indicate an important interaction of fibroblasts and mast cells, via SCF and MCP-1, in the induction of fibrosis...
The registry of the German Network for Systemic Scleroderma: frequency of disease subsets and patterns of organ involvementN Hunzelmann
Department of Dermatology and Venerology, University of Cologne, Kerpener Str 62, 50924 Cologne, Germany
Rheumatology (Oxford) 47:1185-92. 2008..To improve the detection and follow-up of patients presenting with different disease manifestations, an interdisciplinary registry was founded with contributions from different subspecialties involved in the care of patients with SSc...
Impaired wound healing in embryonic and adult mice lacking vimentinB Eckes
Department of Dermatology, University of Cologne, Koln, Germany
J Cell Sci 113:2455-62. 2000..These observations reveal an in vivo phenotype for the vimentin-deficient mouse, and challenge the dogma that key morphogenetic events occurring during development require generation of significant tractional forces by mesenchymal cells...
BM-40 and MMP-2 expression are not coregulated in human melanoma cell linesR Nischt
Department of Dermatology, University of Cologne, D 50924, Cologne, Germany
Cancer Lett 162:223-30. 2001..This was further corroborated by the finding that exogenous BM-40 failed to alter synthesis and activation of proMMP-2...
Integrin alpha2beta1 is required for regulation of murine wound angiogenesis but is dispensable for reepithelializationManon C Zweers
Department of Dermatology, University of Cologne, Cologne, Germany
J Invest Dermatol 127:467-78. 2007..Both ultrastructurally and functionally, these new blood vessels appeared intact. In conclusion, our data show unique and overlapping functions of alpha2beta1 integrin during murine cutaneous wound healing...
High expression and autoinduction of monocyte chemoattractant protein-1 in scleroderma fibroblastsT Yamamoto
Department of Dermatology, University of Cologne, Cologne, Germany
Eur J Immunol 31:2936-41. 2001..Moreover, increased responsiveness of scleroderma fibroblasts to MCP-1 could result in a continuation of the fibrotic response...
Interleukin 4 and prolonged hypoxia induce a higher gene expression of lysyl hydroxylase 2 and an altered cross-link pattern: important pathogenetic steps in early and late stage of systemic scleroderma?J Brinckmann
Department of Dermatology, University of Lubeck, Ratzeburger Allee 160, 23538 Lubeck, Germany
Matrix Biol 24:459-68. 2005....
Ras-induced spreading and wound closure in human epidermal keratinocytesMichael Tscharntke
Department Dermatology, University of Cologne and Centre for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
FASEB J 19:1836-8. 2005..Our results suggest that growth factor-induced, Ras-mediated changes of keratinocyte shape may be an important mechanism that determines the speed of wound epithelialization...
Pathogenic role for skin macrophages in a mouse model of keratinocyte-induced psoriasis-like skin inflammationAthanasios Stratis
Department of Dermatology and Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
J Clin Invest 116:2094-104. 2006....
Interleukin-10 derived from macrophages and/or neutrophils regulates the inflammatory response to LPS but not the response to CpG DNALisa Siewe
Department of Dermatology, University of Cologne, Cologne, Germany
Eur J Immunol 36:3248-55. 2006..These results show that different innate immune responses can be subject to control by IL-10 from different cellular sources...
Plasmin modulates vascular endothelial growth factor-A-mediated angiogenesis during wound repairDetlev Roth
Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, 50931 Koln, Germany
Am J Pathol 168:670-84. 2006..Inactivation of the plasmin cleavage site Arg110/Ala111 may preserve the biological function of VEGF165 in therapeutic angiogenesis under conditions in which proteases are highly active, such as wound repair and inflammation...
Expanding the keratin mutation database: novel and recurrent mutations and genotype-phenotype correlations in 28 patients with epidermolytic ichthyosisM J Arin
Department of Dermatology, University of Cologne, Kerpener Strasse 62, 50931 Cologne, Germany
Br J Dermatol 164:442-7. 2011..The presence of palmoplantar keratoderma suggests KRT1 mutations, whereas KRT10 mutations in most instances give rise to the nonpalmoplantar variants...
Increased deposition of fibulin-2 in solar elastosis and its colocalization with elastic fibresN Hunzelmann
Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, 50924 Cologne, Germany
Br J Dermatol 145:217-22. 2001..Fibulin-2 is a 195-kDa protein belonging to a novel family of extracellular matrix proteins that might be involved in microfibril and elastic fibre organization...
Circulating type I collagen degradation products: a new serum marker for clinical severity in patients with scleroderma?N Hunzelmann
Department of Dermatology, University of Cologne, Germany
Br J Dermatol 139:1020-5. 1998....
[Current pathophysiological aspects of systemic sclerosis]T Krieg
Klinik und Poliklinik fur Dermatologie und Venerologie, Universitat zu Koln, Joseph Stelzmann Strasse 9, 50924, Koln, Germany
Z Rheumatol 65:275-8. 2006..This review describes the major advancemades during recent years for the understanding of the pathophysiology of systemic scleroderma...
[Chronic wounds. Novel approaches in research and therapy]S A Eming
Klinik und Poliklinik fur Dermatologie und Venerologie, Universitat zu Koln, Joseph Stelzmann Strasse 9, 50931 Koln
Hautarzt 58:939-44. 2007..This review focuses on new developments in wound healing research and the resulting non-operative therapeutic implications...
[Organ-specific diagnosis in patients with systemic sclerosis: Recommendations of the German Network for Systemic Sclerosis (DNSS)]N Hunzelmann
Klinik und Poliklinik fur Dermatologie und Venerologie, Klinikum der Universität zu Köln, 50924 Köln, Deutschland
Z Rheumatol 67:334-6, 337-40. 2008....
Molecular mechanisms of VEGF-A action during tissue repairSabine A Eming
Department of Dermatology, University of Cologne, Cologne, Germany
J Investig Dermatol Symp Proc 11:79-86. 2006..These observations support the concept that VEGF-A and VEGF-A receptors are important mediators in tissue repair. Further, our data provide mechanisms how VEGF-A-mediated interactions are disturbed during impaired healing...
Dissecting the roles of endothelin, TGF-beta and GM-CSF on myofibroblast differentiation by keratinocytesPierre Shephard
Department of Dermatology, University of Cologne, Germany
Thromb Haemost 92:262-74. 2004..Our results dissect the roles of TGF-beta and ET-1 on mechanical force generation in keratinocyte-fibroblast co-cultures, and identify GM-CSF as an inducer of myofibroblasts acting indirectly...
A human keratin 10 knockout causes recessive epidermolytic hyperkeratosisFelix B Müller
Department of Dermatology, University of Cologne, 50924 Köln, Germany
Hum Mol Genet 15:1133-41. 2006..Identification of the heterogeneity of this disorder has a major impact for the accurate genetic counseling of patients and their families and also has implications for gene-therapy approaches...
Interactions of primary fibroblasts and keratinocytes with extracellular matrix proteins: contribution of alpha2beta1 integrinZhi Gang Zhang
Department of Dermatology, Medical Faculty, University of Cologne, 50931 Cologne, Germany
J Cell Sci 119:1886-95. 2006..The deficits observed for alpha2-deficient fibroblasts appeared to be linked to alterations in the distribution of force-bearing focal adhesions and deregulation of Rho-GTPase activation...
Novel and recurrent mutations in keratin KRT5 and KRT14 genes in epidermolysis bullosa simplex: implications for disease phenotype and keratin filament assemblyFelix B Müller
Department of Dermatology, University of Cologne, Cologne, Germany
Hum Mutat 27:719-20. 2006..Detailed knowledge of the spectrum of EBS mutations and their genotype-phenotype correlation is essential for accurate genetic counselling and prenatal diagnosis...
T cell-specific inactivation of the interleukin 10 gene in mice results in enhanced T cell responses but normal innate responses to lipopolysaccharide or skin irritationAxel Roers
Dept of Dermatology, University of Cologne, Josef Stelzmann Str 9, 50931 Cologne, Germany
J Exp Med 200:1289-97. 2004..Our data highlight the importance of T cell-derived IL-10 in the regulation of T cell responses and demonstrate that endotoxic shock and the irritant response of the skin are controlled by IL-10 from other cell types...
Silicone granuloma of the face treated with minocyclineMeral J Arin
Department of Dermatology, University of Cologne, Cologne, Germany
J Am Acad Dermatol 52:53-6. 2005..Treatment of siliconomas is difficult and granulomas involving the face are a therapeutic challenge. We present a 43-year-old woman with a severely disfiguring facial silicone granuloma who was successfully treated with minocycline...
Inflammation in wound repair: molecular and cellular mechanismsSabine A Eming
Department of Dermatology, University of Cologne, Cologne, Germany
J Invest Dermatol 127:514-25. 2007..In this paper, we review cellular and molecular mechanisms controlling inflammation in cutaneous tissue repair and provide a rationale for targeting the inflammatory phase in order to modulate the outcome of the healing response...
Impaired epidermal wound healing in vivo upon inhibition or deletion of Rac1Michael Tscharntke
Department of Dermatology, University of Cologne, Center for Molecular Medicine, CMMC, Joseph Stelzmann Strasse 9, 50924 Cologne, Germany
J Cell Sci 120:1480-90. 2007..We conclude that Rac1 is functionally required for normal epidermal wound healing and, in this context, exerts a dual function - namely the regulation of keratinocyte proliferation and migration...
Scleroderma: from pathophysiology to novel therapeutic approachesNicolas Hunzelmann
Department of Dermatology, University of Cologne, Koln, Germany
Exp Dermatol 19:393-400. 2010..g. tyrosine kinase inhibitors, are currently being investigated for the treatment of this life-threatening disease...
Differential proteomic analysis distinguishes tissue repair biomarker signatures in wound exudates obtained from normal healing and chronic woundsSabine A Eming
Department of Dermatology, Center for Molecular Medicine Cologne CMMC, Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases CECAD, Institute for Oral and Musculoskeletal Biology, University of Cologne, Cologne, Germany
J Proteome Res 9:4758-66. 2010..This analysis therefore represents an important basis for the search for potential biomarkers, which give rise to a better understanding and monitoring of disease progression in chronic wounds...
Increased expression of VEGF in glomeruloid reactive angioendotheliomatosisSabine A Eming
Department of Dermatology, University of Cologne, Cologne, Germany
Dermatology 207:398-401. 2003..This case suggests a possible role of epidermally derived VEGF in endothelial cell proliferation in RAE...
Merkel cell polyomavirus DNA in persons without merkel cell carcinomaUlrike Wieland
Institute of Virology, University of Cologne, Koeln, Germany
Emerg Infect Dis 15:1496-8. 2009..MCPyV was also found in anogenital and oral samples (31%) and eyebrow hairs (50%) of HIV-positive men and in forehead swabs (62%) of healthy controls. MCPyV thus appears to be widespread...
Long-term efficacy, curative potential, and prognostic factors of radiotherapy in primary cutaneous B-cell lymphomaHans Theodor Eich
Department of Radiation Oncology, University of Cologne, Cologne, Germany
Int J Radiat Oncol Biol Phys 55:899-906. 2003..To evaluate the efficacy of RT, patient data from two centers were analyzed and compared with recent reports in the literature...
Accelerated wound closure in mice deficient for interleukin-10Sabine A Eming
Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, 50931 Koln, Germany
Am J Pathol 170:188-202. 2007..These results show for the first time that IL-10 can impede wound repair...
Gene transfer in tissue repair: status, challenges and future directionsSabine A Eming
University of Cologne, Department of Dermatology, Cologne, Joseph Stelzmann Str 9, 50931 Koln, Germany
Expert Opin Biol Ther 4:1373-86. 2004..This article summarises repair mechanisms and their failure, and gives an overview of techniques and studies applied to gene transfer in tissue repair. It also provides perspectives on potential targets for gene transfer technology...
Interrelation of immunity and tissue repair or regenerationSabine A Eming
Department of Dermatology, University of Cologne, Germany
Semin Cell Dev Biol 20:517-27. 2009..Finally, we report on new mechanistic insights on how these inflammatory pathways impair healing under pathological conditions and discuss therapeutic implications...
Biomarkers for skin involvement and fibrotic activity in sclerodermaP Moinzadeh
Department of Dermatology, Medical Faculty, University of Cologne, Cologne, Germany
J Eur Acad Dermatol Venereol 26:267-76. 2012....
Bullous pemphigoid in a patient treated with UVA-1 phototherapy for disseminated morpheaC Sacher
Department of Dermatology, University of Cologne, Germany
Dermatology 202:54-7. 2001..Immunosuppressive therapy resulted in regression of all blisters. After ruling out other possible causes, such as neoplasias or drugs, we conclude that UVA-1 has to be regarded as the most likely trigger of the disease...
CD40L induces matrix-metalloproteinase-9 but not tissue inhibitor of metalloproteinases-1 in cervical carcinoma cells: imbalance between NF-kappaB and STAT3 activationS Smola-Hess
Institute of Virology, University of Cologne, Fürst Pückler Str 56, Cologne, 50935, Germany
Exp Cell Res 267:205-15. 2001..Selective CD40-mediated signaling through NF-kappaB but not STAT3 correlates to a shift of the balance between MMP-9 and TIMP-1 toward the protease...
Differential roles of macrophages in diverse phases of skin repairTina Lucas
Department of Dermatology, University of Cologne, Cologne, Germany
J Immunol 184:3964-77. 2010..These results demonstrate that macrophages exert distinct functions during the diverse phases of skin repair, which are crucial to control the natural sequence of repair events...
Mechanical tension and integrin alpha 2 beta 1 regulate fibroblast functionsBeate Eckes
Department of Dermatology, University of Cologne, Cologne, Germany
J Investig Dermatol Symp Proc 11:66-72. 2006..Unraveling the integrated functions of fibroblasts, collagen integrin receptors, collagen fibrils, and mechanical tension will be important to understand the molecular mechanisms underlying tissue repair and fibrosis...
Localized inflammatory skin disease following inducible ablation of I kappa B kinase 2 in murine epidermisAthanasios Stratis
Department of Dermatology, University of Cologne and Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
J Invest Dermatol 126:614-20. 2006..Our results identify a primary role for IKK2 in the development of skin inflammation and confirm its requirement for the maintenance of skin homeostasis...
TNF-mediated inflammatory skin disease in mice with epidermis-specific deletion of IKK2Manolis Pasparakis
Institute for Genetics, University of Cologne, Weyertal 121, D 50931 Cologne, Germany
Nature 417:861-6. 2002..Our results suggest that the critical function of IKK2-mediated NF-kappa B activity in epidermal keratinocytes is to regulate mechanisms that maintain the immune homeostasis of the skin...
Myofibroblast differentiation is induced in keratinocyte-fibroblast co-cultures and is antagonistically regulated by endogenous transforming growth factor-beta and interleukin-1Pierre Shephard
Departments of Dermatology and Virology, University of Cologne, Cologne, Germany
Am J Pathol 164:2055-66. 2004..This biphasic differentiation event is regulated by the balance of endogenous TGF-beta and IL-1 activity and is reminiscent of myofibroblast differentiation at early and later stages of wound healing...
Identification of novel and known KRT5 and KRT14 mutations in 53 patients with epidermolysis bullosa simplex: correlation between genotype and phenotypeM J Arin
Department of Dermatology, University of Cologne, Kerpener Strasse 62, 50924 Cologne, Germany
Br J Dermatol 162:1365-9. 2010..Basal epidermolysis bullosa simplex (EBS) is a hereditary skin blistering disorder resulting in most cases from missense mutations in the keratin 5 (KRT5) or keratin 14 (KRT14) genes...
Comparison of patients with and without digital ulcers in systemic sclerosis: detection of possible risk factorsC Sunderkotter
Department of Dermatology, University of Munster, Von Esmarch Str 58, 48149 Munster, Germany
Br J Dermatol 160:835-43. 2009..For optimal management it would be useful to identify the risk factors for DU. Such statistical analyses have been rare because they require a high number of patients...
Early increase in serum levels of the angiogenesis-inhibitor endostatin and of basic fibroblast growth factor in melanoma patients during disease progressionP Kurschat
Department of Dermatology and Center for Molecular Medicine CMMC, University of Cologne, Josef Stelzmann Str 9, 50924 Cologne, Germany
Br J Dermatol 156:653-8. 2007..Therefore, these factors have been suggested as diagnostic and in some studies as prognostic tumour markers...
Enhanced contact allergen- and UVB-induced keratinocyte apoptosis in the absence of CD95/Fas/Apo-1A Hedrych-Ozimina
Department of Dermatology, Center for Molecular Medicine, University of Cologne, Joseph Stelzmann Strasse 9, Cologne 50924, Germany
Cell Death Differ 18:155-63. 2011....
Intermediate molecular weight proteinuria and albuminuria identify scleroderma patients with increased morbidityB Seiberlich
Department of Medicine I, Merheim Medical Center, Cologne General Hospital, Cologne, Germany
Clin Nephrol 70:110-7. 2008..Renal involvement and systemic vascular damage have been shown to significantly affect prognosis in systemic sclerosis (SSc). However, it is often difficult to assess damage to the renal and systemic vasculature in SSc patients...
[Molecular mechanisms of healing]S Jurk
Department of Dermatology, University of Cologne, Joseph Stelzmann Str. 9, 50931 Cologne, Allemagne
Ann Dermatol Venereol 130:574-80. 2003
Extensive human papillomavirus type 7-associated orofacial warts in an immunocompetent patientA Ritzkowsky
Department of Dermatology, University of Cologne, Germany
Acta Derm Venereol 81:130-3. 2001..Our data show that HPV7 can lead to perioral, spiky warts and brownish plaques in immunocompetent patients who had never been working as a meat or fish handler...
BM-40 (osteonectin, SPARC) is expressed both in the epidermal and in the dermal compartment of adult human skinN Hunzelmann
Department of Dermatology, University of Cologne, Germany
J Invest Dermatol 110:122-6. 1998..This study demonstrates that BM-40 that has previously been thought to be exclusively expressed in extracellular matrix producing cells may in fact play a role in differentiation and maintenance of the epidermis...
Bath-PUVA therapy in three patients with scleredema adultorumC M Hager
Department of Dermatology, University of Cologne, Germany
J Am Acad Dermatol 38:240-2. 1998..Scleredema adultorum (SA) is a rare connective tissue disorder for which no treatment has proven to be effective...
Collagen and collagenase gene expression in three-dimensional collagen lattices are differentially regulated by alpha 1 beta 1 and alpha 2 beta 1 integrinsO Langholz
Department of Dermatology, University of Cologne, Germany
J Cell Biol 131:1903-15. 1995..The data presented here indicate that different signaling pathways exist leading to the three events discussed here, and that these pathways do not per se depend upon each other...
The absence of nidogen 1 does not affect murine basement membrane formationM Murshed
Department of Dermatology, Medical Faculty, University of Cologne, Germany
Mol Cell Biol 20:7007-12. 2000..The results show that nidogen 1 is not required for basement membrane formation or maintenance...
Anti-CD20 monoclonal antibody (rituximab) in the treatment of pemphigusM J Arin
Department of Dermatology, University of Cologne, 50924 Cologne, Germany
Br J Dermatol 153:620-5. 2005..CONCLUSIONS: This study highlights the prolonged effect and disease control after one single course of rituximab and further extends the spectrum of treatments of bullous autoimmune disorders...
Impaired mechanical stability, migration and contractile capacity in vimentin-deficient fibroblastsB Eckes
Department of Dermatology, University of Cologne, Federal Republic of Germany
J Cell Sci 111:1897-907. 1998....
Generation of a novel proteolysis resistant vascular endothelial growth factor165 variant by a site-directed mutation at the plasmin sensitive cleavage siteGereon Lauer
Department of Dermatology, University of Cologne, Cologne, Germany
FEBS Lett 531:309-13. 2002..These results suggest that the plasmin cleavage site Arg110/Ala111 and the carboxyl-terminal domain play an important role in the mitogenic activity of VEGF165...
Treatment of chronic wounds: state of the art and future conceptsSabine A Eming
Department of Dermatology, University of Cologne, Germany
Cells Tissues Organs 172:105-17. 2002....
Morphometric analysis of murine skin wound healing: standardization of experimental procedures and impact of an advanced multitissue array techniqueMichael Gerharz
Institute of Pathology, University of Cologne, Cologne, Germany
Wound Repair Regen 15:105-12. 2007..For the analysis of cellular composition in GT, multitissue arrays are useful tools in wound-healing studies...
Expression of matrilin-2 in human skinDorothea Piecha
Department of Dermatology, Medical Faculty, University of Cologne, Joseph Stelzmann Strasse 9, 50931 Cologne, Germany
J Invest Dermatol 119:38-43. 2002..These findings demonstrate that matrilin-2 is expressed in normal skin by keratinocytes and fibroblasts and may thus contribute to cutaneous homeostasis...
E-cadherin is essential for in vivo epidermal barrier function by regulating tight junctionsJudith A Tunggal
Center for Molecular Medicine, University of Cologne CMMC, Cologne, Germany
EMBO J 24:1146-56. 2005..Surprisingly, our results indicate that E-cadherin is specifically required for tight junction, but not desmosome, formation and this appears to involve signalling rather than cell contact formation...
Terminal B cell differentiation is skewed by deregulated interleukin-6 secretion in beta2 integrin-deficient miceThorsten Peters
Department of Dermatology, and Allergic Diseases, University of Ulm, Maienweg 12, 89081 Ulm, Germany
J Leukoc Biol 80:599-607. 2006..It may be of interest to examine human leukocyte-adhesion deficiency type-1 patients closer and search for pathological changes possibly induced via overproduction of IL-6...
Alternative proteolytic processing of hepatocyte growth factor during wound repairNils Buchstein
Professor of Dermatology, Department of Dermatology, University of Cologne, Joseph Stelzmann Str 9, 50931 Koln, Germany
Am J Pathol 174:2116-28. 2009....
Signalling and regulation of collagen I synthesis by ET-1 and TGF-beta1Angelika Horstmeyer
Department of Dermatology, University of Cologne, Cologne, Germany
FEBS J 272:6297-309. 2005..Thereby, long-time stimulation by ET-1 resulted in a changed ET-receptor subtype ratio and in a biphasic CTGF induction...
Gene therapy and wound healingSabine A Eming
Department of Dermatology, University of Cologne, D 50937 Cologne, Germany
Clin Dermatol 25:79-92. 2007..We have summarized the molecular and cellular basis of repair mechanisms and their failure, and we give an overview of techniques and studies applied to gene transfer in tissue repair...
Adjuvant treatment with vindesine in comparison to observation alone in patients with metastasized melanoma after complete metastasectomy: a randomized multicenter trial of the German Dermatologic Cooperative Oncology GroupThomas K Eigentler
Department of Dermatology, Skin Cancer Program, Eberhard Karls University, Tuebingen, Germany
Melanoma Res 18:353-8. 2008..Thus, this randomized trial did not confirm earlier reports of beneficial effects of adjuvant vindesine and can therefore not be recommended...
Toll-like receptor-4 deficiency attenuates doxorubicin-induced cardiomyopathy in miceAlexander Riad
Charite Universitatsmedizin Berlin, Germany, Campus Benjamin Franklin, Department of Cardiology and Pneumology, Hindenburgdamm 30, 12200 Berlin, Germany
Eur J Heart Fail 10:233-43. 2008..Since TLR4 might play a relevant role in cardiac inflammatory signalling, we investigated whether or not TLR4 is involved in Dox-induced cardiotoxicity...
Mast cell-specific Cre/loxP-mediated recombination in vivoJulia Scholten
Department of Dermatology, University of Cologne, Kerpener Str 62, 50937, Cologne, Germany
Transgenic Res 17:307-15. 2008..Our results show that the Mcpt5 promoter can drive Cre expression in a mast cell-specific fashion. We expect that our Mcpt5-Cre mice will be a useful tool for the investigation of mast cell biology...
CD4+ T cell-associated pathophysiology critically depends on CD18 gene dose effects in a murine model of psoriasisDaniel Kess
Department of Dermatology, University of Cologne, Cologne, Germany
J Immunol 171:5697-706. 2003..This murine inflammatory skin model may also have relevance for human polygenic inflammatory diseases...
Transcriptional profiling identifies an interferon-associated host immune response in invasive squamous cell carcinoma of the skinJoerg Wenzel
Department of Dermatology, University of Bonn, Bonn, Germany
Int J Cancer 123:2605-15. 2008..Collectively, our findings support the concept that IFN-associated host responses play an important role in tumor immunosurveillance in the skin...
Role of postoperative radiotherapy in the management of Merkel cell carcinomaHans Theodor Eich
Department of Radiotherapy, University of Cologne, Joseph-Stelzmann Strasse 9, D-50924 Cologne, Germany
Am J Clin Oncol 25:50-6. 2002..Postoperative radiotherapy to the primary tumor region and the regional lymphatics is effective in the prevention of locoregional recurrence. Prospective clinical trials should be performed to confirm these observations...
Expression of matrix metalloproteinase (MMP)-2, MMP-9, MMP-13, and MT1-MMP in skin tumors of human papillomavirus type 8 transgenic miceBaki Akgul
Institute of Virology, University of Cologne, Germany
Exp Dermatol 15:35-42. 2006..Taken together, these results implicate a role for MMPs in the development of HPV8-induced cutaneous tumors...
Scleroderma -- news to tellBeate Eckes
Department of Dermatology, University of Cologne, Kerpener Str 62, Cologne, Germany
Arch Dermatol Res 299:139-44. 2007..Systematic analysis of the pathways involved in the development of the disease together with information derived from novel animal models have helped to better understand the mechanisms that trigger and sustain fibrotic reactions...
Gene expression profiling of lichen planus reflects CXCL9+-mediated inflammation and distinguishes this disease from atopic dermatitis and psoriasisJoerg Wenzel
Department of Dermatology, University of Bonn, Bonn, Germany
J Invest Dermatol 128:67-78. 2008..Gene expression profiling might complement the diagnostic spectrum in dermatology and may provide new pathogenetic insights...
High invasive melanoma cells induce matrix metalloproteinase-1 synthesis in fibroblasts by interleukin-1alpha and basic fibroblast growth factor-mediated mechanismsStefanie Löffek
Department of Dermatology, Center of Molecular Medicine CMMC, University of Cologne, Cologne, Germany
J Invest Dermatol 124:638-43. 2005..Taken together, our results indicate an essential role for soluble factors, mainly IL-1alpha and bFGF, in the stimulation of dermal fibroblasts by human melanoma cells to secrete MMP-1...
Expression of pro-inflammatory markers by human dermal fibroblasts in a three-dimensional culture model is mediated by an autocrine interleukin-1 loopDaniela Kessler-Becker
Department of Dermatology, University of Cologne, Joseph-Stelzmann-Strasse 9, D-50931 Cologne, Germany
Biochem J 379:351-8. 2004..We propose that fibroblasts that are embedded in a matrix environment can actively participate in the regulation of inflammatory processes...
Trichilemmal cyst nevus: a new complex organoid epidermal nevusIliana Tantcheva Poor
Department of Dermatology, University of Cologne, Cologne, Germany
J Am Acad Dermatol 57:S72-7. 2007..Consequently, we propose for this new organoid nevus the names "trichilemmal cyst nevus" or "nevus trichilemmocysticus."..
Integrin alpha2beta1 deficiency does not affect contact hypersensitivityManon C Zweers
Department of Dermatology, University of Cologne, Kerpener Str. 62, 50937 Cologne, Germany
Arch Dermatol Res 298:201-5. 2006..Our results suggest that the adhesive functions of integrin alpha2beta1 are dispensable for the CHS response; they may be compensated for by the collagen receptor alpha1beta1 or other collagen receptors...
High plasminogen activator inhibitor type 2 expression is a hallmark of scleroderma fibroblasts in vitroDaniela Kessler-Becker
Department of Dermatology, University of Cologne, Cologne, Germany
Exp Dermatol 13:708-14. 2004..The data indicate that scleroderma fibroblasts are more sensitive to the induction of PAI-2 expression than control fibroblasts by a presently unknown factor in serum...
Overexpression of phospholipid-hydroperoxide glutathione peroxidase in human dermal fibroblasts abrogates UVA irradiation-induced expression of interstitial collagenase/matrix metalloproteinase-1 by suppression of phosphatidylcholine hydroperoxide-mediateJutta Wenk
Department of Dermatology, University of Cologne, 50924 Cologne, Germany
J Biol Chem 279:45634-42. 2004..Our finding is particularly relevant in light of the already available small molecule mimetics of PHGPx...
Complete remission of Merkel cell carcinoma of the scalp with local and regional metastases after topical treatment with dinitrochlorbenzolGernot Herrmann
Department of Dermatology, University of Cologne, Cologne, Germany
J Am Acad Dermatol 50:965-9. 2004..Together with subsequent irradiation, remission has now lasted for more than 1 year...
Identification of activated matrix metalloproteinase-2 (MMP-2) as the main gelatinolytic enzyme in malignant melanoma by in situ zymographyPeter Kurschat
Department of Dermatology, University of Cologne, Cologne, Germany
J Pathol 197:179-87. 2002..This technology makes it possible to draw conclusions regarding the balance between activated proteases and inhibitors, which are frequently found to be present together in close proximity in vivo...
The inhibition of matrix metalloproteinase activity in chronic wounds by a polyacrylate superabsorberSabine Eming
Department of Dermatology, University of Cologne, Kerpener Strasse 62, 50924 Cologne, Germany
Biomaterials 29:2932-40. 2008..We conclude that polyacrylate superabsorber particles can rescue the highly proteolytic microenvironment of non-healing wounds from MMP activity so that more conductive conditions allow healing to proceed...
Defective laminin 5 processing in cylindroma cellsLucy Tunggal
Department of Dermatology, Medical Faculty, Institute for Biochemistry II, University of Cologne, Joseph-Stelzmann-Strasse 52, , Germany
Am J Pathol 160:459-68. 2002..Together, the results show that multiple molecular defects, including alteration of laminin 5 and its integrin receptors, contribute to structural aberrations of the basement membrane and associated structures in cylindromas...
Absence of autoantibodies against correctly folded recombinant fibrillin-1 protein in systemic sclerosis patientsJurgen Brinckmann
Department of Dermatology, University of Lubeck, Lubeck, Germany
Arthritis Res Ther 7:R1221-6. 2005....
Integrin alpha 2-deficient mice develop normally, are fertile, but display partially defective platelet interaction with collagenOlaf Holtkötter
Department of Dermatology, University of Cologne, Cologne, Germany
J Biol Chem 277:10789-94. 2002..Thus, alpha(2)beta(1) plays a supportive rather than an essential role in platelet-collagen interactions. These results are in agreement with the observation that alpha(2)beta(1)-deficient animals suffer no bleeding anomalies...
Contact of high-invasive, but not low-invasive, melanoma cells to native collagen I induces the release of mature cathepsin BAnke Klose
Department of Dermatology, Center for Molecular Medicine CMMC, University of Cologne, Cologne, Germany
Int J Cancer 118:2735-43. 2006..These results strongly implicate a pivotal role of cell-matrix interactions for the regulation of cathepsin B localization and activity in melanoma cells...
