Research Topics
Genomes and Genes | R GebhardtSummaryAffiliation: University of Leipzig Country: Germany Publications
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Publications
Inhibition of matrix metalloproteinase-2 and -9 activities by selected flavonoidsChristina Ende
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Planta Med 70:1006-8. 2004..Our results add a novel function to the long list of biological effects of these ubiquitous plant constituents that may contribute to and enhance their modulating influence on extracellular matrix degradation and remodelling...
Organ patterning in the adult stage: the role of Wnt/beta-catenin signaling in liver zonation and beyondRolf Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Dev Dyn 239:45-55. 2010....
Carnosine retards tumor growth in vivo in an NIH3T3-HER2/neu mouse modelChristof Renner
Klinik und Poliklinik fur Neurochirurgie, Universitätsklinikum Leipzig und Medizinische Fakultät der Universität Leipzig, Leipzig, Germany
Mol Cancer 9:2. 2010..In the present work we investigated whether carnosine also affects tumor growth in vivo and may therefore be considered for human cancer therapy...
Correlation between beta-catenin mutations and expression of Wnt-signaling target genes in hepatocellular carcinomaMadeleine Austinat
Institute for Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
Mol Cancer 7:21. 2008....
New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation proceduresRolf Gebhardt
Institute of Biochemistry, University of Leipzig, Leipzig, Germany
Drug Metab Rev 35:145-213. 2003..Representative results with test substances and recommendations for application of these in vitro systems, as well as standard operation procedures are given...
Antioxidative, antiproliferative and biochemical effects in HepG2 cells of a homeopathic remedy and its constituent plant tinctures tested separately or in combinationRolf Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Arzneimittelforschung 53:823-30. 2003....
Anticholestatic activity of flavonoids from artichoke (Cynara scolymus L.) and of their metabolitesR Gebhardt
Institut fur Biochemie, Universitatsklinikum Leipzig, Germany
Med Sci Monit 7:316-20. 2001..These results demonstrate that artichoke leaf extracts exert a potent anticholestatic action at least in the case of taurolithocholate-induced cholestasis. Flavonoids and their metabolites may contribute significantly to this effect...
4-Aminoethylamino-emodin--a novel potent inhibitor of GSK-3beta--acts as an insulin-sensitizer avoiding downstream effects of activated beta-cateninRolf Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
J Cell Mol Med 14:1276-93. 2010....
Hepatocellular expression of glutamine synthetase: an indicator of morphogen actions as master regulators of zonation in adult liverRolf Gebhardt
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Johannisallee 30, 04103 Leipzig, Germany
Prog Histochem Cytochem 41:201-66. 2007....
Variable influence of kaempferol and myricetin on in vitro hepatocellular cholesterol biosynthesisR Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Planta Med 69:1071-4. 2003..These opposing effects on cholesterol biosynthesis are exerted in an indirect manner and seem possible though differential modulation of the complex regulation of HMGCoA reductase...
Enhanced matrix degradation after withdrawal of TGF-beta1 triggers hepatocytes from apoptosis to proliferation and regenerationE Arendt
Institute of Biochemistry, University of Leipzig, Leipzig, Germany
Cell Prolif 38:287-99. 2005....
Reduction and expansion of the glutamine synthetase expressing zone in livers from tetracycline controlled TGF-beta1 transgenic mice and multiple starved miceElke Ueberham
Institute of Biochemistry, Medical Faculty, University of Leipzig, Liebigstrasse 16, D 04103 Leipzig, Germany
J Hepatol 41:75-81. 2004....
Prevention of taurolithocholate-induced hepatic bile canalicular distortions by HPLC-characterized extracts of artichoke (Cynara scolymus) leavesR Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Planta Med 68:776-9. 2002..These results demonstrate that artichoke leaf extracts exert a potent anticholestatic action at least in the case of taurolithocholate. This effect may contribute to the overall hepatoprotective influence of this herbal formulation...
Oval cell proliferation in p16INK4a expressing mouse liver is triggered by chronic growth stimuliElke Ueberham
Institute of Biochemistry, University of Leipzig, Medical Faculty, Leipzig, Germany
J Cell Mol Med 12:622-38. 2008..We could show that the liver specific expression of p16(INK4a) leads to a faster differentiation of hepatocytes and an activation of oval cells already in postnatal mice without negative consequences on liver function...
Conditional tetracycline-regulated expression of TGF-beta1 in liver of transgenic mice leads to reversible intermediary fibrosisElke Ueberham
University of Leipzig, Institute for Biochemistry, Germany
Hepatology 37:1067-78. 2003..In conclusion, this model will enable the analysis of fibrogenesis at progressive stages and help in elucidating the cellular changes during development and regression of liver fibrosis caused by elevated TGF-beta1 expression...
Long-term expression of foreign genes in normal human epidermal keratinocytes after transfection with lipid/DNA complexesS Zellmer
Institute of Biochemistry, Medical Faculty, University Leipzig, Germany
Histochem Cell Biol 115:41-7. 2001..Transfected keratinocytes, cultivated at the air-liquid interface, expressed the marker gene beta-galactosidase for at least 7 weeks...
An intronic silencer element is responsible for specific zonal expression of glutamine synthetase in the rat liverFrank Gaunitz
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Leipzig, Germany
Hepatology 41:1225-32. 2005....
Comparison of uptake and neuroprotective potential of seven zinc-saltsSanja Pavlica
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Leipzig, Germany
Neurochem Int 56:84-93. 2010..The differences in accumulation of zinc-salts were not specific and unique to PC12 cells, since similar results were obtained in rat primary hepatocytes and endothelial HUVEC cells...
E-cadherin as a reliable cell surface marker for the identification of liver specific stem cellsElke Ueberham
Institute of Biochemistry, Medical Faculty, University of Leipzig, Johannisallee 30, 04103 Leipzig, Germany
J Mol Histol 38:359-68. 2007..In human cirrhotic liver samples E-cadherin expression was found as a common feature of both, typical and atypical reactions, and, thus, can also serve as an indication of the progenitor cell compartment activation...
Inducible neuronal expression of transgenic TGF-beta1 in vivo: dissection of short-term and long-term effectsUwe Ueberham
Paul Flechsig Institute for Brain Research, Department of Neuroanatomy, University of Leipzig, Jahnallee 59, D 04109 Leipzig, Germany
Eur J Neurosci 22:50-64. 2005..Neuroprotective effects were observed after short-term expression of TGF-beta1. Death of striatal neurons induced by 3-nitropropionic acid was markedly reduced after induced TGF-beta1 expression...
Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented dietElke Ueberham
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Comp Hepatol 9:8. 2010....
Transcription factors ETF, E2F, and SP-1 are involved in cytokine-independent proliferation of murine hepatocytesSebastian Zellmer
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Hepatology 52:2127-36. 2010..The transcription factors ETF, E2F1, and SP-1 seem to play a pronounced role in mediating proliferation-dependent differential gene expression. Similar events, but on a shorter time-scale, occur very early after liver damage in vivo...
Heterogeneous expression of suppressor of cytokine signalling 2 (SOCS-2) in liver tissueSebastian Zellmer
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
J Anat 215:176-83. 2009..Presumably, the heterogeneous distribution of SOCS-2 may contribute to the pericentral preference of growth hormone action via differential feedback...
Hepatic expression of glutamine synthetase in rats is controlled by STAT5 and TCF transcription factorsMax Werth
, , , Leipzig, Germany
Hepatology 44:967-75. 2006..In conclusion, the GS 5' enhancer contains elements important for GS expression in cells carrying an activated form of beta-catenin as previously shown in experimentally induced hepatocellular carcinomas...
Glucocorticoid induced expression of glutamine synthetase in hepatoma cellsFrank Gaunitz
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany
Biochem Biophys Res Commun 296:1026-32. 2002..However, evidence is presented that aside from a primary effect on transcription glucocorticoids mediate their effect on the expression of GS also at the posttranscriptional level...
A silencer element in the first intron of the glutamine synthetase gene represses induction by glucocorticoidsFrank Gaunitz
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Liebigstrasse 16, 04103 Leipzig, Germany
Mol Endocrinol 18:63-9. 2004..This yet unknown factor was designated GS silencer-binding protein. It is absent in FAO cells that respond to glucocorticoids with enhanced expression of GS and present in HepG2 cells that do not respond...
Dioxin mediates downregulation of the reduced folate carrier transport activity via the arylhydrocarbon receptor signalling pathwaySandra Halwachs
Institute of Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary Medicine, University of Leipzig, Leipzig, Germany
Toxicol Appl Pharmacol 246:100-6. 2010....
Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytesA Garten
University Hospital for Children and Adolescents, University of Leipzig, Germany
Biochem Biophys Res Commun 391:376-81. 2010..In primary human hepatocytes, secreted NAMPT was less active. Our results demonstrate that human hepatocytes are a potential source of circulating NAMPT...
Synthesis and biological evaluation of new derivatives of emodinLars Teich
, , Pharmazie und Psychologie, , , 04103 Leipzig, Germany
Bioorg Med Chem 12:5961-71. 2004....
Mathematical modelling of liver regeneration after intoxication with CCl(4)Stefan Höhme
Interdisciplinary Centre for Bioinformatics, University of Leipzig, Hartelstr 16 18, D 04107 Leipzig, Germany
Chem Biol Interact 168:74-93. 2007....
Curcumin inhibits glyoxalase 1: a possible link to its anti-inflammatory and anti-tumor activityThore Santel
Institute of Biochemistry, University of Leipzig, Leipzig, Germany
PLoS ONE 3:e3508. 2008..Inhibitors of glyoxalases are considered as anti-inflammatory and anti-carcinogenic agents. The recent finding that various polyphenols modulate Glo1 activity has prompted us to assess curcumin's potency as an Glo1 inhibitor...
Inhibition of cholesterol biosynthesis in HepG2 cells by artichoke extracts is reinforced by glucosidase pretreatmentRolf Gebhardt
, , 04103 Leipzig, Germany
Phytother Res 16:368-72. 2002..These findings emphasize the importance of beta-glucosidase-dependent liberation of luteolin for the ability of artichoke extracts to inhibit hepatic cholesterol biosynthesis...
Protective effects of flavonoids and two metabolites against oxidative stress in neuronal PC12 cellsSanja Pavlica
Institute of Biochemistry, Medical Faculty, University of Leipzig, 04103 Leipzig, Germany
Life Sci 86:79-86. 2010....
Identification of a cis-acting element and a novel trans-acting factor of the glutamine synthetase gene in liver cellsF Gaunitz
Medizinische Fakultat, Institut fur Biochemie, Universitat Leipzig, Liebigstrasse 16, Leipzig, 04103, Germany
Biochem Biophys Res Commun 284:377-83. 2001..This nuclear protein, designated as upstream binding factor of the GS gene (UFGS) seems to play an important role in high-level expression of GS in liver...
Oxidative stress, plant-derived antioxidants and liver fibrosisR Gebhardt
Institut of Biochemistry, University of Leipzig, Germany
Planta Med 68:289-96. 2002..This review briefly summarizes current views of the mechanisms of fibrogenesis and recent findings on the antifibrotic potential of plant-derived antioxidants...
Acquisition of blood--tissue barrier--supporting features by hepatic stellate cells and astrocytes of myofibroblastic phenotype. Inverse dynamics of metallothionein and glial fibrillary acidic protein expressionG H Buniatian
Institut fur Biochemie, Universitat Leipzig, Medizinische Facultät, Liebig Str 16, D 04103 Leipzig, Germany
Neurochem Int 38:373-83. 2001..In addition, this study shows that, at least in cell culture, an enrichment of perivascular cells in GFAP results in the disappearance of protective functions...
Protective effects of ellagic and chlorogenic acids against oxidative stress in PC12 cellsSanja Pavlica
, , , Liebigstr. 16, Leipzig, 04103, Germany
Free Radic Res 39:1377-90. 2005..These findings suggest that two dietary antioxidants, EA and CGA, may have protective properties against oxidative stress induced in CNS...
Alpha2-macroglobulin inhibits the malignant properties of astrocytoma cells by impeding beta-catenin signalingInge Lindner
Institute of Biochemistry, Department of Ophthalmology, University of Leipzig, Leipzig, Germany
Cancer Res 70:277-87. 2010..Notably, LRP1 antibodies could phenocopy the effects of A2M*. Our findings suggest a pathway of tumor suppression in astrocytoma that might be tractable to therapeutic exploitation...
In vitro screening of plant extracts and phytopharmaceuticals: novel approaches for the elucidation of active compounds and their mechanismsR Gebhardt
Institut fur Biochemie, Medizinische Fakultat, Universität Leipziq, Germany
Planta Med 66:99-105. 2000..Furthermore, they may give full insight into not only the therapeutic potential but also the possible dangers of phytopharmaceuticals and may eventually lead to hints on new therapeutic fields that can be further explored...
A novel model of fibroblast-mediated cartilage destructionU Sack
Institute of Clinical Immunology and Transfusion Medicine, University of Leipzig, Leipzig, Germany
Scand J Immunol 61:18-28. 2005....
Influence of organosulphur compounds from garlic on the secretion of matrix metalloproteinases and their inhibitor TIMP-1 by cultured HUVEC cellsK Meyer
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Cell Biol Toxicol 20:253-60. 2004..These results suggest that DADS may mediate some of the biological effects ascribed to garlic preparations through affecting MMP-TIMP balance...
From teratogens to potential therapeutics: natural inhibitors of the Hedgehog signaling network come of ageAmalya Hovhannisyan
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Planta Med 75:1371-80. 2009....
Comparative in vitro toxicity of seven zinc-salts towards neuronal PC12 cellsSanja Pavlica
Institut fur Biochemie, Medizinische Fakultat, Universitat Leipzig, Johannisallee 30, 04103 Leipzig, Germany
Toxicol In Vitro 23:653-9. 2009..In conclusion, Zn(2+)-salts could be divided into three categories with high (zinc-citrate, zinc-sulphate), moderate (zinc-orotate, zinc-acetate, zinc-chloride(,) zinc-gluconate) and low cytotoxicity (zinc-histidinate)...
Functional interactions of Cu-ATPase ATP7B with cisplatin and the role of ATP7B in the resistance of cells to the drugKaroline Leonhardt
Department of Medicine II and Department of Biochemistry, University of Leipzig, 04103 Leipzig, Germany
J Biol Chem 284:7793-802. 2009..The link between changes in copper homeostasis and cisplatin resistance was confirmed by treating the Huh7 cells with copper chelator and increasing their resistance to cisplatin.cisplatin...
Co-cultivation of liver epithelial cells with hepatocytesRolf Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Methods Mol Biol 188:337-46. 2002
Prediction and validation of cell alignment along microvessels as order principle to restore tissue architecture in liver regenerationStefan Hoehme
Interdisciplinary Centre for Bioinformatics IZBI, University of Leipzig, Härtelstrasse 16 18, D 04107 Leipzig, Germany
Proc Natl Acad Sci U S A 107:10371-6. 2010..The results of this analysis clearly confirmed the model prediction. We believe our procedure is widely applicable in the systems biology of tissues...
Influence of biotransformation of luteolin, luteolin 7-O-glucoside, 3',4'-dihydroxyflavone and apigenin by cultured rat hepatocytes on antioxidative capacity and inhibition of EGF receptor tyrosine kinase activityDoreen Schlupper
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Planta Med 72:596-603. 2006..These results show that the biotransformation of flavonoids is very complex and may result not only in a loss but also in a gain of biological activity depending on the individual structural features...
Liver epithelial cell cultureRolf Gebhardt
Institute of Biochemistry, Medical Faculty, University of Leipzig, Leipzig, Germany
Methods Mol Biol 188:53-63. 2002
Carnosine inhibits ATP production in cells from malignant gliomaChristof Renner
Klinik und Poliklinik fur Neurochirurgie, Medizinische Fakultät der Universität Leipzig, Leipzig, Germany
Neurol Res 32:101-5. 2010..In order to understand how this effect is mediated, experiments were performed that addressed a possible influence of carnosine on energy metabolism...
Antioxidant properties of silybin glycosidesPavel Kosina
Centre for Bioanalytical Research, , , 775 15 Olomouc, Czech Republic
Phytother Res 16:S33-9. 2002..These results suggest that silybin glycosides are suitable soluble derivatives of silybin for experimental studies and may have therapeutic potential...
The RL-ET-14 cell line mediates expression of glutamine synthetase through the upstream enhancer/promoter regionMarianna Kruithof-de Julio
AMC Liver Center, Academic Medical Center, Amsterdam, The Netherlands
J Hepatol 43:126-31. 2005..5 kb and that the signal mediator required a functional beta-catenin pathway. CONCLUSIONS: The 'RL-ET-14' factor specifically induces GS expression, working via its upstream enhancer in a beta-catenin-dependent fashion...
Hepatocyte-specific Smad7 expression attenuates TGF-beta-mediated fibrogenesis and protects against liver damageSteven Dooley
Department of Medicine II, Gastroenterology and Hepatology, University Hospital, Mannheim, Germany
Gastroenterology 135:642-59. 2008..The profibrogenic role of transforming growth factor (TGF)-beta in liver has mostly been attributed to hepatic stellate cell activation and excess matrix synthesis. Hepatocytes are believed to contribute to increased rates of apoptosis...
Profibrogenic transforming growth factor-beta/activin receptor-like kinase 5 signaling via connective tissue growth factor expression in hepatocytesHong Lei Weng
Molecular Alcohol Research in Gastroenterology, Department of Medicine II, Faculty of Medicine at Mannheim, University of Heidelberg, Mannheim, Germany
Hepatology 46:1257-70. 2007..We identify ALK5-dependent Smad3 signaling as the responsible pathway inducing CTGF expression, which can be hindered by an activated activin receptor-like kinase 1 pathway and completely inhibited by TGF-beta antagonist Smad7...
Effect of garlic powder on C-reactive protein and plasma lipids in overweight and smoking subjectsMartijn B A van Doorn
TNO Prevention and Health, Gaubius Laboratory, Leiden, Netherlands
Am J Clin Nutr 84:1324-9. 2006..Epidemiologic studies suggest that garlic may have beneficial effects on risk factors associated with cardiovascular disease (CVD). However, these findings are not unambiguously supported by randomized placebo-controlled clinical trials...
Overexpression of glutamine synthetase is associated with beta-catenin-mutations in mouse liver tumors during promotion of hepatocarcinogenesis by phenobarbitalSandra Loeppen
, , Germany
Cancer Res 62:5685-8. 2002..These data suggest that promotion of hepatocarcinogenesis by PB confers beta-catenin-mutated tumor cells with a selective advantage by up-regulation of GS expression...
Investigation of the membrane localization and distribution of flavonoids by high-resolution magic angle spinning NMR spectroscopyHolger A Scheidt
Junior Research Group Solid-state NMR Studies of the Structure of Membrane-associated Proteins, Biotechnological-Biomedical Center of the University of Leipzig, Liebigstr. 27, D-04103 Leipzig, Germany
Biochim Biophys Acta 1663:97-107. 2004..From the localization of different flavonoid protons in the membrane, a model for the orientation of the molecules in a lipid bilayer can be deduced. This orientation depends on the position of the polar center of the flavonoid molecule...
Zonal gene expression in murine liver: Are tumors helping us to solve the mystery?Rolf Gebhardt
Hepatology 44:512; author reply 512-3. 2006
High circulating leptin receptors with normal leptin sensitivity in liver-specific insulin receptor knock-out (LIRKO) miceShmuel E Cohen
Joslin Diabetes Center, Boston, Massachusetts 02215, USA
J Biol Chem 282:23672-8. 2007..In this manner, insulin signaling in liver plays an important role in leptin homeostasis and fine modulation of leptin action...
The oxidative biotransformation of losoxantrone (CI-941)Ulf D Renner
Medizinische Klinik I, Universitatsklinikum Carl Gustav Carus, Dresden, Germany
Drug Metab Dispos 30:464-78. 2002..Furthermore, the CI-941 metabolism was shown to be extremely suppressed in rat hepatocytes incubated with metyrapone. In contrast to these results, human tumor HepG2 cells did not show any CI-941 biotransformation after incubation...
Cadmium, cobalt and lead cause stress response, cell cycle deregulation and increased steroid as well as xenobiotic metabolism in primary normal human bronchial epithelial cells which is coordinated by at least nine transcription factorsFelix Glahn
Institute of Environmental Toxicology, Martin Luther University, Halle Wittenberg, Halle Saale, Germany
Arch Toxicol 82:513-24. 2008..This study gives an overview of the most responsive genes...
