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Genomes and Genes | Hans Joachim GabiusSummaryAffiliation: University of Munich Country: Germany Publications
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Publications
Chemical biology of the sugar codeHans Joachim Gabius
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, Veterinarstrasse 13, 80539 Munich, Germany
Chembiochem 5:740-64. 2004....- Identification of Siglec-9 as the receptor for MUC16 on human NK cells, B cells, and monocytesJennifer A Belisle
Department of Obstetrics and Gynecology, University of Wisconsin Madison, Madison, WI, USA
Mol Cancer 9:118. 2010..In a previous study we demonstrated that sMUC16 binds to specific subset of NK cells. Here, we identify the csMUC16/sMUC16 binding partner expressed on immune cells... - A regulatory network of two galectins mediates the earliest steps of avian limb skeletal morphogenesisRamray Bhat
Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY 10595, USA
BMC Dev Biol 11:6. 2011..The formation of these condensations depends on cell-matrix and cell-cell interactions, but how they are initiated and patterned is as yet unresolved... - Biological information transfer beyond the genetic code: the sugar codeH J Gabius
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Germany
Naturwissenschaften 87:108-21. 2000..They embody clinical applications reaching from receptor localization in diagnostic pathology to cell type-selective targeting of drugs and inhibition of undesired cell adhesion in bacterial/viral infections, inflammation, or metastasis... - Glycobiomarkers by glycoproteomics and glycan profiling (glycomics): emergence of functionalityHans Joachim Gabius
Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, 80539 Munich, Germany
Biochem Soc Trans 39:399-405. 2011..Thus studies on glycobiomarkers converge with research on how distinct carbohydrate determinants are turned into bioactive signals... - Glycans: bioactive signals decoded by lectinsHans Joachim Gabius
Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Munich, Germany
Biochem Soc Trans 36:1491-6. 2008..In this sense, orchestration of glycan and lectin expression is an efficient means, with far-reaching relevance, to exploit the coding potential of oligosaccharides physiologically and medically... - Cell surface glycans: the why and how of their functionality as biochemical signals in lectin-mediated information transferHans Joachim Gabius
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, D 80539 Munchen, Germany
Crit Rev Immunol 26:43-79. 2006..This primer to the fundamentals of the sugar code, with telling examples from the realm of immunology, also illustrates the strategic and hitherto unsuspected fine-tuning between glycan structure and lectin design/expression... - From lectin structure to functional glycomics: principles of the sugar codeHans Joachim Gabius
Institute of Physiological Chemistry, Ludwig Maximilians University Munich, Munchen, Germany
Trends Biochem Sci 36:298-313. 2011..Experimentally, the strategic combination of methods to monitor distinct aspects of the lectin-glycan interplay offers a promising perspective to answer this question... - Glycohistochemistry: the why and how of detection and localization of endogenous lectinsH J Gabius
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstr 13, D 80539 Munchen, Germany
Anat Histol Embryol 30:3-31. 2001.... - Probing the cons and pros of lectin-induced immunomodulation: case studies for the mistletoe lectin and galectin-1H J Gabius
Lehrstuhl für Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, 80539 Munich, Germany
Biochimie 83:659-66. 2001.... - Unique conformer selection of human growth-regulatory lectin galectin-1 for ganglioside GM1 versus bacterial toxinsHans Christian Siebert
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstrasse 13, 80539 Munchen, Germany
Biochemistry 42:14762-73. 2003..In principle, our methodology can be applied in studies aimed at blocking galectin functionality in malignancy and beyond glycosciences... - The sugar code: functional lectinomicsHans Joachim Gabius
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstr 13, D 80539, Munich, Germany
Biochim Biophys Acta 1572:165-77. 2002.... - Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silico and in vitro on human neuroblastoma cellsSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, Munich, Germany
Int J Cancer 114:46-57. 2005..Moreover, the given strategy for comparative computational analysis of extended binding sites has implications for the rational design of galectin-type-specific ligands... - Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycanSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Germany
FEBS J 272:1986-98. 2005..The results argue in favor of the concept of viewing substitutions as molecular switches for lectin-binding affinity. Moreover, they have potential relevance for glycoengineering of reagents in tumor imaging... - Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivoSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munich, Germany
Eur J Biochem 271:118-34. 2004..Synthetic glycan tailoring provides a versatile approach to the preparation of newly substituted glycans with favorable ligand properties for medical applications... - Branching mode in complex-type triantennary N-glycans as regulatory element of their ligand propertiesSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Veterinaerstr 13, 80539 Munich, Germany
Biochim Biophys Acta 1760:768-82. 2006..This part of the study is relevant for rational glycoengineering of pharmaproteins. In general, our study supports the concept to view details of N-glycan structure, here branching, as a means to modulate ligand properties... - Prototype chicken galectins revisited: characterization of a third protein with distinctive hydrodynamic behaviour and expression pattern in organs of adult animalsHerbert Kaltner
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstr 13, D 80539 Munchen, Germany
Biochem J 409:591-9. 2008..Overall, the results reveal a network of three prototype galectins in chicken... - Negative regulation of RPE cell attachment by carbohydrate-dependent cell surface binding of galectin-3 and inhibition of the ERK-MAPK pathwayClaudia S Alge-Priglinger
Department of Ophthalmology, Ludwig Maximilians University, Mathildenstrasse 8, Munich, Germany
Biochimie 93:477-88. 2011..Further investigation at this pathway may disclose a promising nouveau perspective for treatment and prophylaxis of early PVR... - Suitability of binary mixtures of water with aprotic solvents to turn hydroxyl protons of carbohydrate ligands into conformational sensors in NOE and transferred NOE experimentsHans Christian Siebert
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstr 13, 80539 Munchen, Germany
J Biomol NMR 25:197-215. 2003..Our experiments reveal that binary mixtures have favorable properties for the conformational analysis of the free- and bound-state topologies of bioactive ligands... - Toward comprehensive analysis of the galectin network in chicken: unique diversity of galectin-3 and comparison of its localization profile in organs of adult animals to the other four members of this lectin familyHerbert Kaltner
Faculty of Veterinary Medicine, Institute of Physiological Chemistry, Ludwig Maximilians University, Veterinarstrasse 13, Munich, Germany
Anat Rec (Hoboken) 294:427-44. 2011..This information on CG-3 completes the description of the panel of CGs, hereby setting the stage for detailed comparative analysis of the entire CG family, e.g., in embryogenesis... - Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targetsSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, D 80539 Munich, Germany
Bioconjug Chem 15:87-98. 2004..This type of chemical scaffold with favorable pharmacologic properties might thus be exploited for the design of galectin- and ligand-type-selective glycoclusters... - Substitutions in the N-glycan core as regulators of biorecognition: the case of core-fucose and bisecting GlcNAc moietiesSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstrasse 13, D 80539 Munich, Germany
Biochemistry 46:6984-95. 2007..Moreover, our data inspire us to devise new, non-natural modifications to realize the full potential of glycoengineering for diagnostic and therapeutic purposes... - Combining carbohydrate substitutions at bioinspired positions with multivalent presentation towards optimising lectin inhibitors: case study with calixarenesSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, Veterinarstr 13, 80539 Munchen, Germany
Chem Commun (Camb) 47:6126-8. 2011..In detail, aromatic substituents were introduced at the 2-N or 3'-positions of N-acetyllactosamine and the products conjugated to two types of calix[n]arenes (n = 4, 6) via thiourea-linker chemistry... - Evidence for lectin activity of a plant receptor-like protein kinase by application of neoglycoproteins and bioinformatic algorithmsSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Veterinarstr 13, 80539 Munchen, Germany
Biochim Biophys Acta 1725:222-32. 2005..These results give further research direction to define physiological ligands, plant/bacterial rhamnose-containing saccharides and rhamnose-mimetic glycans or peptides being potential candidates... - Carbamate-linked lactose: design of clusters and evidence for selectivity to block binding of human lectins to (neo)glycoproteins with increasing degree of branching and to tumor cellsSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, Veterinarstrasse 13, 80539 Munchen, Germany
Bioconjug Chem 20:1716-28. 2009..The reported bioactivity and the delineation of its modulation by structural parameters of lectins and glycans set instructive examples for the further design of selective inhibitors and assay procedures... - Analysis of protein-carbohydrate interaction at the lower size limit of the protein part (15-mer peptide) by NMR spectroscopy, electrospray ionization mass spectrometry, and molecular modelingHans Christian Siebert
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstrasse 13, 80539 Munchen, Germany
Biochemistry 41:9707-17. 2002..We have thereby proven effectiveness of our approach to analyze even low-affinity interactions (not restricted to carbohydrates as ligands). It will be useful to evaluate the impact of rational manipulation of lead peptide sequences... - Carbohydrate chain of ganglioside GM1 as a ligand: identification of the binding strategies of three 15 mer peptides and their divergence from the binding modes of growth-regulatory galectin-1 and cholera toxinHans Christian Siebert
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstrasse 13, 80539 Munchen, Germany
Chemistry 12:388-402. 2005.... - Unique sequence and expression profiles of rat galectins-5 and -9 as a result of species-specific gene divergenceMartin Lensch
Institute for Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, D 80539 Munich, Germany
Int J Biochem Cell Biol 38:1741-58. 2006..The presented results on galectin-5 relative to galectin-9 intimate distinct functions especially in erythropoiesis and imply currently unknown mechanisms to compensate its absence from the galectin network in other mammals... - Tumor suppressor p16INK4a--modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cellsSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Germany
FEBS J 274:3233-56. 2007..This mechanism establishes a new aspect of p16(INK4a) functionality... - Calix[n]arene-based glycoclusters: bioactivity of thiourea-linked galactose/lactose moieties as inhibitors of binding of medically relevant lectins to a glycoprotein and cell-surface glycoconjugates and selectivity among human adhesion/growth-regulatory gSabine Andre
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat, Veterinarstrasse 13, 80539 Munchen, Germany
Chembiochem 9:1649-61. 2008..The results thus revealed bioactivity of galactose-/lactose-presenting calix[n]arenes for medically relevant lectins and selectivity within the family of adhesion/growth-regulatory human galectins... - Galectins as tools for glycan mapping in histology: comparison of their binding profiles to the bovine zona pellucida by confocal laser scanning microscopyFelix A Habermann
Institute of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, Ludwig Maximilians University, Veterinarstr 13, 80539, Munich, Germany
Histochem Cell Biol 135:539-52. 2011..Our results encourage further comparative analysis and research to trace the underlying structural and/or topological properties... - Cyclic neoglycodecapeptides: how to increase their inhibitory activity and selectivity on lectin/toxin binding to a glycoprotein and cellsSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Munich, Germany
J Pept Sci 17:427-37. 2011..Cyclic neoglycodecapeptides thus warrant further study as lectin-directed pharmaceuticals... - The emerging functionality of endogenous lectins: A primer to the concept and a case study on galectins including medical implicationsHans Joachim Gabius
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Germany
Chang Gung Med J 29:37-62. 2006..Further insights into the functional consequences of the sugar code's translation are thus expected to have notable repercussions for diagnostic and therapeutic procedures... - Phenylenediamine-based bivalent glycocyclophanes: synthesis and analysis of the influence of scaffold rigidity and ligand spacing on lectin binding in cell systems with different glycomic profilesSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, 80539 Munich, Germany
Org Biomol Chem 7:4715-25. 2009..The applied strategy for the cell assays is proposed to be of general importance in the quest to identify medically relevant lectin inhibitors... - Inhibition of human retinal pigment epithelial cell attachment, spreading, and migration by the human lectin galectin-1Claudia S Alge-Priglinger
Department of Ophthalmology, Ludwig Maximilians University, Munich, Germany
Mol Vis 15:2162-73. 2009..The role of the endogenous lectin, galectin-1, in attachment, spreading, and migration of human RPE cells was investigated from a therapeutic perspective... - Cell-type-specific expression of murine multifunctional galectin-3 and its association with follicular atresia/luteolysis in contrast to pro-apoptotic galectins-1 and -7Michaela Lohr
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University, Munich, Germany
Histochem Cell Biol 130:567-81. 2008..This study demonstrates cell-type specificity and cycle-associated regulation for galectin-3 with increased presence in atretic preantral follicles and in late stages of luteolysis... - Unique chicken tandem-repeat-type galectin: implications of alternative splicing and a distinct expression profile compared to those of the three proto-type proteinsHerbert Kaltner
Institut fur Physiologische Chemie, Tierarztliche Fakultat, Ludwig Maximilians Universitat Munchen, Veterinarstrasse 13, D 80539 Munchen, Germany
Biochemistry 48:4403-16. 2009..Overall, we extend the network of CGs to a tandem-repeat-type protein and provide a detailed characterization from gene and protein structures to expression... - Discovery of galectin ligands in fully randomized combinatorial one-bead-one-compound (glyco)peptide librariesSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, 80539 Munich, Germany
Bioorg Med Chem Lett 17:793-8. 2007..The various data obtained define the library approach as a facile route for the discovery of selective (glyco)peptide-based galectin inhibitors... - From structural to functional glycomics: core substitutions as molecular switches for shape and lectin affinity of N-glycansSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, D 80539 Munich, Germany
Biol Chem 390:557-65. 2009..These modifications are thus molecular switches... - Synthesis of bivalent lactosides and their activity as sensors for differences between lectins in inter- and intrafamily comparisonsSabine Andre
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig Maximilians University Munich, Veterinarstr 13, 80539 Munich, Germany
Bioorg Med Chem Lett 22:313-8. 2012..Two of the bivalent probes can be considered as sensors for interlectin differences. Most pronounced were the selectivities of N-glycosyl 1,2,3-triazole derivatives for the chimera-type galectin and its proteolytically truncated version... - alpha2,3/alpha2,6-Sialylation of N-glycans: non-synonymous signals with marked developmental regulation in bovine reproductive tractsHans-Christian Siebert
Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, , , Germany
Biochimie 88:399-410. 2006..They give each disaccharide its own characteristic meaning as signal in the sugar code... - Structure-activity profiles of complex biantennary glycans with core fucosylation and with/without additional alpha 2,3/alpha 2,6 sialylation: synthesis of neoglycoproteins and their properties in lectin assays, cell binding, and organ uptakeCarlo Unverzagt
Institut fur Organische Chemie und Biochemie, Technische Universitat Munchen, Lichtenbergstrasse 4, 85748 Garching, Germany
J Med Chem 45:478-91. 2002..Overall, our study underscores the importance of chemoenzymatic synthesis to define the effect of chain orientation on the ligand properties of N-glycans... - Effects of polyvalency of glycotopes and natural modifications of human blood group ABH/Lewis sugars at the Galbeta1-terminated core saccharides on the binding of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Kwei San, Tao Yuan 333, Taiwan, ROC
Biochimie 86:317-26. 2004.... - Analysis of selected blood and immune cell responses to carbohydrate-dependent surface binding of proto- and chimera-type galectinsAlexander V Timoshenko
Institute of Photobiology, National Academy of Sciences of Belarus, Minsk
Mol Cell Biochem 250:139-49. 2003..Within the family of galectins, the activity profiles depend on the target cell type and the individual galectin. Notably, proto-type galectins do not necessarily share a uniform capacity as elicitor... - Chromosomal aberrations, profiles of expression of growth-related markers including galectins and environmental hazards in relation to the incidence of chondroid pulmonary hamartomasKlaus Kayser
UICC Telepathology Consultation Center, Institute of Pathology, Charite, Humboldt University, Berlin, Germany
Pathol Res Pract 199:589-98. 2003..These results contribute to the definition of biochemical characteristics in hamartomas and can be useful for distinguishing them from chronic degenerative disorders... - Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cellsJurgen Kopitz
Institut für Molekulare Pathologie, Klinikum der Ruprecht Karls Universtiät, Im Neuenheimer Feld 220, 69120 Heidelberg, Germany
Oncogene 22:6277-88. 2003..In addition to p53-linked proapoptotic activity intracellularly, galectin-7, acting as a lectin on the cell surface, appears to be capable of reducing cancer cell proliferation in susceptible systems... - Molecular biological fingerprinting of human lectin expression by RT-PCRHarald Lahm
Immunology-Molecular Biology Laboratory, Thoraxklinik Heidelberg gGmbHm, Amalienstrasse 5, Heidelberg D-69126, Germany
Methods Enzymol 362:287-97. 2003 - The asialoglycoprotein receptor clears glycoconjugates terminating with sialic acid alpha 2,6GalNAcEric I Park
Department of Pathology, Washington University School of Medicine, St Louis, MO 63110, USA
Proc Natl Acad Sci U S A 102:17125-9. 2005.... - Describing topology of bound ligand by transferred nuclear Overhauser effect spectroscopy and molecular modelingHans-Christian Siebert
Institute of Physiological Chemistry, Ludwig-Maximilians University, Veterinarstrasse 13, Munich D-80539, Germany
Methods Enzymol 362:417-34. 2003 - Detection of ligand- and solvent-induced shape alterations of cell-growth-regulatory human lectin galectin-1 in solution by small angle neutron and x-ray scatteringLizhong He
Institute for Coastal Research, Physical and Chemical Analysis, Geesthacht, Germany
Biophys J 85:511-24. 2003..We have hereby proven that ligand presence and an aprotic solvent significantly affect the shape of galectin-1 in solution... - Binding sites for Lewis antigens are expressed by human colon cancer cells and negatively affect their migrationAxel Hittelet
Department of Gastroenterology, Erasmus University Hospital, Faculty of Medicine, , Jules Bordet Institute, Brussels, Belgium
Lab Invest 83:777-87. 2003..Binding sites for Lewis epitopes are present on colon cancer cells. The functional relevance of these sites is indicated by the negative influence on cell migration of a matrix containing the oligosaccharides as ligand parts... - Galectin-3, an endogenous lectin, as a tool for monitoring cell differentiation in head and neck carcinomas with implications for lectin-glycan functionalityJan Betka
Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Faculty Hospital Motol, Prague, Czech Republic
Acta Otolaryngol 123:261-3. 2003..The aim of this study was to monitor the carbohydrate-dependent binding of labeled galectin-3 to primary head and neck squamous cell carcinomas (from the tonsil, base of the tongue and larynx) and lymph node metastases... - Upregulation of galectins-1 and -3 in human colon cancer and their role in regulating cell migrationAxel Hittelet
Laboratory of Gastroenterology, Erasmus Hospital, Brussels, Belgium
Int J Cancer 103:370-9. 2003..15 microg/cm(2) gal-1, and the presence of a blocking antibody at least reduced this effect. gal-3 significantly reduced cell migration in all 4 of the in vitro cell lines... - Fine specificity of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N)Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Biochem J 367:653-64. 2002..The definition of this binding profile provides the basis to detect differential binding properties relative to the other galectins with ensuing implications for functional analysis... - Galectin-1 is overexpressed in nasal polyps under budesonide and inhibits eosinophil migrationCarine Delbrouck
E N T Department, Erasmus University Hospital, Brussels, Belgium
Lab Invest 82:147-58. 2002..The present study therefore reveals a new galectin-1-mediated mechanism of action for glucocorticoid-mediated anti-inflammatory effects... - Interference of the galactose-dependent binding of lectins by novel pentapeptide ligandsChristopher J Arnusch
Utrecht Institute for Pharmaceutical Sciences, Department of Medicinal Chemistry, Utrecht University, PO Box 80082, 3508 TB Utrecht, The Netherlands
Bioorg Med Chem Lett 14:1437-40. 2004..Sequences were identified that modulate galectin binding to its natural carbohydrate ligand. SPR showed inhibition values 2-3 times stronger than galactose and NMR studies suggested real carbohydrate mimicry... - Domain versatility in plant AB-toxins: evidence for a local, pH-dependent rearrangement in the 2gamma lectin site of the mistletoe lectin by applying ligand derivatives and modellingMarta Jimenez
Instituto de Quimica Fisica Rocasolano, Consejo Superior de Investigaciones Cientificas, Serrano 119, Madrid, Spain
FEBS Lett 582:2309-12. 2008..Using these results and molecular dynamics simulations, a local conformational change was rendered likely. The obtained structural information is valuable for the design of potent inhibitors... - Towards functional glycomics by localization of binding sites for tissue lectins: lectin histochemical reactivity for galectins during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamsterSven Saussez
Laboratory of Histology, Faculty of Medicine and Pharmacy, University of Mons Hainaut, Avenue du Champ de Mars, 6 Pentagone 1B, 7000, Mons, Belgium
Histochem Cell Biol 126:57-69. 2006.... - alpha2,6-Sialylation promotes binding of placental protein 14 via its Ca2+-dependent lectin activity: insights into differential effects on CD45RO and CD45RA T cellsEliran Ish-Shalom
Goldyne Savad Institute of Gene Therayp, Hadassah Hebrew University Medical Center, Jerusalem, Israel
Glycobiology 16:173-83. 2006..Finally, we demonstrate that PP14 promotes CD45 dimerization and clustering, a phenomenon that may regulate CD45 activity... - Prognostic significance of endogenous adhesion/growth-regulatory lectins in lung cancerTamas Szoke
Department of Surgery, University of Szeged, Szeged, Hungary
Oncology 69:167-74. 2005..To determine the expression of endogenous adhesion/growth-regulatory lectins and their binding sites using labeled tissue lectins as well as the binding profile of hyaluronic acid as an approach to define new prognostic markers... - Activity-structure correlations in divergent lectin evolution: fine specificity of chicken galectin CG-14 and computational analysis of flexible ligand docking for CG-14 and the closely related CG-16Albert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, College of Medicine, Chang Gung University, Kwei San, Tao Yuan, Taiwan
Glycobiology 17:165-84. 2007..In summary, this study identifies activity differences and provides information on their relation to structural divergence, epitomizing the value of this combined approach beyond galectins... - Detection of S100B, S100A6 and galectin-3 ligands in meningiomas as markers of aggressivenessSabine Hancq
Department of Neurosurgery, Erasmus University Hospital, Brussels, Belgium
Int J Oncol 25:1233-40. 2004..Our results thus suggest that the galectin-3-binding sites and S100B (and S100A6 to a lesser extent) could play a role in the aggressiveness characterizing atypical meningiomas... - Carbohydrate recognition systems in autoimmunityEdit I Buzas
Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
Autoimmunity 39:691-704. 2006..This review briefly summarizes data on sensor systems for carbohydrate epitopes and implications for autoimmunity... - The conformation of the C-glycosyl analogue of N-acetyl-lactosamine in the free state and bound to a toxic plant agglutinin and human adhesion/growth-regulatory galectin-1Víctor García Aparicio
Ecole Normale Superieure, Departement de Chimie, UMR CNRS 8642, 24, rue Lhomond, 75231 Paris Cedex 05, France
Carbohydr Res 342:1918-28. 2007..This endogenous lectin recognizes a local minimum of 1, the syn-PhiPsi conformer, and thus a conformational selection process is correlated with the molecular recognition event... - Temperature dependence of ligand-protein complex formation as reflected by saturation transfer difference NMR experimentsPatrick Groves
Department of Protein Science, Centro de Investigaciones Biologicas, CSIC, Ramiro de Maeztu 9, 28040 Madrid, Spain
Magn Reson Chem 45:745-8. 2007..STD-based screening protocols to detect new target-specific compounds may yield a larger number of potential ligands if screened at various temperatures... - Photoderivatized polymer thin films at quartz crystal microbalance surfaces: sensors for carbohydrate-protein interactionsYuxin Pei
Department of Chemistry, KTH Royal Institute of Technology, Teknikringen 30, S 10044 Stockholm, Sweden
Anal Chem 79:6897-902. 2007..The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach... - Galectin-1(L11A) predicted from a computed galectin-1 farnesyl-binding pocket selectively inhibits Ras-GTPBarak Rotblat
Department of Neurobiochemistry, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel
Cancer Res 64:3112-8. 2004..Thus, independently of carbohydrate binding, galectin-1 cooperates with Ras, whereas galectin-1(L11A) inhibits it... - Assessing the inhibitory potency of galectin ligands identified from combinatorial (glyco)peptide libraries using surface plasmon resonance spectroscopyC Elizabeth P Maljaars
Bijvoet Center, Department of Bioorganic Chemistry, Utrecht University, 3584 CH Utrecht, The Netherlands
Anal Biochem 378:190-6. 2008..The presence of lactose in the most effective glycopeptides indicated the presence of affinity-enhancing peptide-lectin contacts. In addition to drug design, they may be helpful for fine-structural analysis of the binding sites... - Up-regulation of galectin-3 and its ligands by Trypanosoma cruzi infection with modulation of adhesion and migration of murine dendritic cellsBernard Vray
Laboratoire d Immunologie Expérimentale, Faculte de Medecine, 808 Route de Lennik, 1070 Brussels, Belgium
Glycobiology 14:647-57. 2004..These results suggest a new immunomodulatory property of T. cruzi... - Interaction profile of galectin-5 with free saccharides and mammalian glycoproteins: probing its fine specificity and the effect of naturally clustered ligand presentationAlbert M Wu
Glyco Immunochemistry Research Laboratory, Institute of Molecular and Cellular Biology, Chang Gung University, Kwei San, Tao Yuan, 333, Taiwan
Glycobiology 16:524-37. 2006..These results are important for the systematic comparison of ligand selection in this family of adhesion/growth-regulatory effectors with potential for medical applications...