Research Topics
Genomes and Genes | Joachim FandreySummaryAffiliation: University of Duisburg-Essen Country: Germany Publications
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Publications
Regulating cellular oxygen sensing by hydroxylationJoachim Fandrey
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Cardiovasc Res 71:642-51. 2006..This review will focus on the recent advances in understanding the hypoxia-induced cellular response with particular respect to cellular O2 sensing for adequate control of HIF-1 activation...- Oxygen sensing and the activation of the hypoxia inducible factor 1 (HIF-1)--invited articleJoachim Fandrey
Institut fuer Physiologie, Universitaet Duisburg Essen, Essen, Germany
Adv Exp Med Biol 648:197-206. 2009..To understand how abundance and activation of HIF-1 is regulated is of fundamental importance as it may open new therapeutic avenues to treat ischemic diseases or cancer where HIF-1 appears to be a key component of the pathophysiology... - Nanoscopy of the cellular response to hypoxia by means of fluorescence resonance energy transfer (FRET) and new FRET softwareChristoph Wotzlaw
Institut fur Physiologie, Universitat Duisburg Essen, D 45122 Essen, Germany
PMC Biophys 3:5. 2010..The abundance and activity of HIF-1 are controlled through posttranslational modification by hydroxylases, the cellular oxygen sensors, of which the activity is oxygen dependent... - Erythropoietin receptors on tumor cells: what do they mean?Joachim Fandrey
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, D 45147 Essen, Germany
Oncologist 13:16-20. 2008..Ongoing studies will help clarify whether the existence of the EPOR has any relevance at all in the cancer setting... - Examining the involvement of erythropoiesis-stimulating agents in tumor proliferation (erythropoietin receptors, receptor binding, signal transduction), angiogenesis, and venous thromboembolic eventsJoachim Fandrey
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
Oncologist 14:34-42. 2009..ESA treatment should be used within labeling confines... - The impact of N-nitrosomelatonin as nitric oxide donor in cell culture experimentsUtta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
J Pineal Res 45:489-96. 2008..In conclusion, NOMela had to be an excellent NO precursor for cells in culture and potentially tissues... - Nuclear oxygen sensing: induction of endogenous prolyl-hydroxylase 2 activity by hypoxia and nitric oxideUtta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, D 45122 Essen, Germany
J Biol Chem 283:31745-53. 2008..Our data suggest a role for PHD2 as a decisive oxygen sensor of the hypoxia-inducible factor degradation pathway within the cell nucleus... - Nitric oxide modulates oxygen sensing by hypoxia-inducible factor 1-dependent induction of prolyl hydroxylase 2Utta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
J Biol Chem 282:1788-96. 2007..In conclusion, we identified the induction of PHD2 as an underlying mechanism of NO-induced degradation of HIF-1alpha... - Hypoxia-inducible factor (HIF) 1alpha accumulation and HIF target gene expression are impaired after induction of endotoxin toleranceStilla Frede
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
J Immunol 182:6470-6. 2009..We conclude that endotoxin tolerance impairs HIF-1alpha induction which reduces the ability of monocytic cells to survive and function under hypoxic conditions... - Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivoChristoph Wotzlaw
Department of Physiology, University of Duisburg Essen, Essen, Germany
Am J Physiol Cell Physiol 301:C266-71. 2011..5 s. type I cells, identified during the experiments by cluster formation in combination with large cell nuclei, seem to respond to hypoxia with heterogeneous kinetics... - Oxygen-sensing under the influence of nitric oxideUtta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, 45122 Essen
Cell Signal 22:349-56. 2010.... - Acute hypoxia induces HIF-independent monocyte adhesion to endothelial cells through increased intercellular adhesion molecule-1 expression: the role of hypoxic inhibition of prolyl hydroxylase activity for the induction of NF-kappa BSandra Winning
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
J Immunol 185:1786-93. 2010.... - Oxygen-regulated expression of the erythropoietin gene in the human renal cell line REPCStilla Frede
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
Blood 117:4905-14. 2011..Using the human kidney cell line REPC, we provide for the first time a powerful tool with which to study the cellular and molecular regulation of renal EPO production... - No evidence for protective erythropoietin alpha signalling in rat hepatocytesThorsten Bramey
Institut fur Physiologische Chemie, Universitatsklinikum Essen, Hufelandstrasse 55, D 45122 Essen, Germany
BMC Gastroenterol 9:26. 2009..The aim of the present work was to study the protective effect of rHu-EPO on warm hypoxia-reoxygenation and cold-induced injury to hepatocytes and the rHu-EPO-dependent signalling involved... - Chelation of cellular calcium modulates hypoxia-inducible gene expression through activation of hypoxia-inducible factor-1alphaUtta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
J Biol Chem 279:44976-86. 2004..Instead, effective chelation of cellular calcium caused the accumulation of HIF-1alpha protein through inhibition of HIF-prolyl hydroxylases and activated HIF-1-dependent gene expression under normoxic conditions... - Monitoring of cellular responses to hypoxiaChristoph Wotzlaw
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
Methods Mol Biol 591:243-55. 2010..Fluorescence resonance energy transfer (FRET) was used to determine the interaction of both subunits in living cells by confocal microscopy... - Molecular imaging: into in vivo interaction of HIF-1alpha and HIF-2alpha with ARNTRebecca Konietzny
Institute for Physiology, University of Duisburg Essen, Essen, Germany
Ann N Y Acad Sci 1177:74-81. 2009..Fusion proteins of HIF subunits and variants of green fluorescent proteins (cyan and yellow fluorescent proteins) were expressed in living cells and protein-protein interactions were imaged in vivo by means of FRET... - Thyroid hormone induces hypoxia-inducible factor 1alpha gene expression through thyroid hormone receptor beta/retinoid x receptor alpha-dependent activation of hepatic leukemia factorTeresa Otto
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Endocrinology 149:2241-50. 2008.... - Non-hypoxic activation of the negative regulatory feedback loop of prolyl-hydroxylase oxygen sensorsSuzan Tug
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Biochem Biophys Res Commun 384:519-23. 2009..Recovery of PHD activity required intact cells and was not observed in cell extracts or recombinant PHD2. In conclusion induction of endogenous PHD2 activity by NO is dependent on a feedback loop initiated despite normoxic conditions... - Optical analysis of the HIF-1 complex in living cells by FRET and FRAPChristoph Wotzlaw
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
FASEB J 21:700-7. 2007..2 nm, but even the N and C terminus of the HIF-1 complex were not further apart than 7.4 nm. Our data suggest a more compact 3-dimensional organization of the HIF complex than described so far by 2-dimensional models... - More insights into the CCN3/Connexin43 interaction complex and its role for signalingAlexandra Gellhaus
Institute of Molecular Biology, University of Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
J Cell Biochem 110:129-40. 2010..Furthermore, the data obtained by FRET analysis allowed us to model the 3D structure of the C-terminus of Cx43 interacting with CCN3... - The proinflammatory cytokine interleukin 1beta and hypoxia cooperatively induce the expression of adrenomedullin in ovarian carcinoma cells through hypoxia inducible factor 1 activationStilla Frede
, , Essen, Germany
Cancer Res 65:4690-7. 2005..Finally, treatment of HIF-1alpha with short interfering RNA revealed a significant role for HIF-1 in the IL-1beta-dependent stimulation of ADM expression... - Bacterial lipopolysaccharide induces HIF-1 activation in human monocytes via p44/42 MAPK and NF-kappaBStilla Frede
Institut fur Physiologie, Universität Duisburg Essen Hufelandstrasse 55, D 45122 Essen, Federal Republic of Germany
Biochem J 396:517-27. 2006..From these results we conclude that LPS critically involves the p44/42 MAPK and NF-kappaB pathway in the activation of HIF-1, which is an important transcription factor for LPS-induced ADM expression... - Hypoxia-induced erythropoietin production: a paradigm for oxygen-regulated gene expressionChristian Stockmann
, , Essen, Germany
Clin Exp Pharmacol Physiol 33:968-79. 2006..In addition to the tight regulation by oxygen tension, temporal and tissue-specific signals limit expression of the EPO gene primarily to the fetal liver and the adult kidney... - Thyroid hormone induces erythropoietin gene expression through augmented accumulation of hypoxia-inducible factor-1Yaluan Ma
, , , Hufelandstrasse 55, D-45122 Essen, Germany
Am J Physiol Regul Integr Comp Physiol 287:R600-7. 2004..Increased synthesis of HIF-1alpha may contribute to the adaptive response of increased oxygen demand under hyperthyroid conditions... - Role of N-acetyl-N-nitroso-tryptophan as nitric oxide donor in the modulation of HIF-1-dependent signalingUtta Berchner-Pfannschmidt
Institut fur Physiologie, Universitat Duisburg Essen, Germany
Biol Chem 391:533-40. 2010..In conclusion, NANT appears to be an excellent NO donor for cells in culture and l-NANT should be useful for in vivo animal studies... - Two-photon imaging of cellular activities in oxygen sensing tissuesChristoph Wotzlaw
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstrasse 55, D 45122 Essen, Germany
Microsc Microanal 14:519-25. 2008.... - Oxygen-dependent and tissue-specific regulation of erythropoietin gene expressionJoachim Fandrey
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, D 45147 Essen, Germany
Am J Physiol Regul Integr Comp Physiol 286:R977-88. 2004..Understanding oxygen and tissue-specific regulation of EPO production is of high relevance for physiology. Moreover, this knowledge might be useful for new therapies to treat human diseases... - The function of hypoxia-inducible factor (HIF) is independent of the endoplasmic reticulum protein OS-9Ulf Brockmeier
Institute of Physiology, University of Duisburg Essen, Essen, Germany
PLoS ONE 6:e19151. 2011..We conclude that OS-9 plays no direct functional role in HIF degradation since physical interaction of OS-9 with oxygen sensing HIF prolyl hydroxylases cannot occur in vivo due to their different subcellular localization... - Combined analysis of hypoxia-inducible factor 1 alpha and metallothionein indicates an aggressive subtype of colorectal carcinomaKlaus Jürgen Schmitz
Institute of Pathology and Neuropathology, University Hospital Essen, University of Duisburg Essen, Hufelandstr 55, 45122 Essen, Germany
Int J Colorectal Dis 24:1287-96. 2009..The present study aimed to analyse the prognostic impact of HIF-1alpha and MT expression in colorectal cancer and to evaluate a possible link of combined HIF-1alpha and MT expression with colorectal cancer progression... - Regulation of hypoxia-inducible factors during inflammationStilla Frede
Institut fur Physiologie, Universitat Duisburg Essen, Essen, Germany
Methods Enzymol 435:405-19. 2007..Prolonged activation of HIF-1 under conditions of inflammation, however, may contribute to the survival of damaged tissue and cells, thus promoting the development of tumors... - The HIF-1 response to simulated ischemia in mouse skeletal muscle cells neither enhances glycolysis nor prevents myotube cell deathNathalie Dehne
Institut fur Physiologie, Universitat Duisburg Essen, Hufelandstr 55, D 45122 Essen, Germany
Am J Physiol Regul Integr Comp Physiol 293:R1693-701. 2007..In contrast, myoblasts survive ischemia and thus may play an important role during regeneration and HIF-1-induced revascularization... - Synthetic transactivation screening reveals ETV4 as broad coactivator of hypoxia-inducible factor signalingKristin Wollenick
Institute of Physiology and Zurich Center for Integrative Human Physiology ZIHP, University of Zurich, 8057 Zurich, Switzerland
Nucleic Acids Res 40:1928-43. 2012..Endogenous ETV4 highly correlated with PHD2, HIF-1/2? and several established markers of tissue hypoxia in 282 human breast cancer tissue samples, corroborating a functional interplay between the ETV4 and HIF pathways... - Visualization of the three-dimensional organization of hypoxia-inducible factor-1 alpha and interacting cofactors in subnuclear structuresUtta Berchner-Pfannschmidt
, , Hufelandstrasse 55, D-45122 Essen, Germany
Biol Chem 385:231-7. 2004..Our data provide information on the nuclear distribution of HIF-1alpha with respect to subnuclear domains that could serve as specific locations for hypoxia-induced gene expression... - High-altitude polycythemiaJoachim Fandrey
, , Hufelandstr. 55, 45122 Essen, Germany
Haematologica 90:1. 2005 - The good, the bad and the ugly in oxygen-sensing: ROS, cytochromes and prolyl-hydroxylasesTill Acker
Neurologisches Institut, Edinger Institut, , Deutschordenstr. 46, D-60528 Frankfurt, Germany
Cardiovasc Res 71:195-207. 2006..Herein, we propose an integrated model for these various oxygen-sensing mechanisms that very efficiently regulate HIF-alpha activity and plasma membrane potassium-channel (PMPC) conductivity... - Hypoxia-induced erythropoietin expression in human neuroblastoma requires a methylation free HIF-1 binding siteJochen Rossler
Department of Pediatric Hematology, Oncology and Endocrinology, Children s Hospital, 45122 Essen, Germany
J Cell Biochem 93:153-61. 2004.... - Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensingEric Metzen
Institute of Physiology, University of Lubeck, Ratzeburger Allee 160, D 23538 Lubeck, Germany
J Cell Sci 116:1319-26. 2003..Endogenous PHD2mRNA and PHD3mRNA were hypoxia-inducible, whereas expression of PHD1mRNA and FIH-1mRNA was oxygen independent. We propose that PHDs and FIH-1 form an oxygen sensor cascade of distinct subcellular localisation... - Oxygen and the cellJoachim Fandrey
Biol Chem 385:203-4. 2004 - A cordial affair - erythropoietin and cardioprotectionJoachim Fandrey
Cardiovasc Res 72:1-2. 2006 - Identification of protein disulfide isomerase as a cardiomyocyte survival factor in ischemic cardiomyopathyAnna Severino
Institute of Cardiology, Catholic University, Rome, Italy
J Am Coll Cardiol 50:1029-37. 2007..The aim of the study was to analyze the molecular mechanisms activated during postinfarction remodeling in human hearts... - Hypoxic pulmonary vasoconstriction is/is not mediated by increased production of reactive oxygen speciesUtta Berchner-Pfannschmidt
J Appl Physiol 101:1000; author reply 1004-5. 2006 - Effects of recombinant human erythropoietin (rHuEPO) on tumor control in patients with cancer-induced anemiaPeter Vaupel
Institut fur Physiologie und Pathophysiologie, Universitat Mainz, Germany
Onkologie 28:216-21. 2005..However, one of the most essential prerequisites for achieving this benefit is the use of rHuEPO in agreement with the evidence-based ASCO/ASH-guidelines recommending a target Hb concentration of 12 g/dl (7.44 mmol/l)... - Hypoxia-inducible erythropoietin gene expression in human neuroblastoma cellsIneke Stolze
Institut für Physiologie der Universität Essen, Dortmund, Germany
Blood 100:2623-8. 2002..Together, SH-SY5Y and Kelly cells may provide a new in vitro model for studying the mechanism of tissue-specific, hypoxia-inducible EPO gene expression... - NCB5OR is a novel soluble NAD(P)H reductase localized in the endoplasmic reticulumHao Zhu
Hematology Division, Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
J Biol Chem 279:30316-25. 2004..The subcellular localization and redox properties of NCB5OR provide important insights into the biology of NCB5OR and the phenotype of the Ncb5or-null mouse... - Nitric oxide impairs normoxic degradation of HIF-1alpha by inhibition of prolyl hydroxylasesEric Metzen
Institute of Physiology, University of Luebeck, Germany
Mol Biol Cell 14:3470-81. 2003..We conclude that GSNO-attenuated prolyl hydroxylase activity accounts for HIF-1alpha accumulation under conditions of NO formation during normoxia and that PHD activity is subject to regulation by NO... - Reactive oxygen species modulate HIF-1 mediated PAI-1 expression: involvement of the GTPase Rac1Agnes Gorlach
Experimentelle Kinderkardiologie, Deutsches Herzzentrum Müchen and der Technischen Universität Müchen, Munich, Germany
Thromb Haemost 89:926-35. 2003..In contrast, NFkappaB activation was enhanced in RacG12V-cells, but abolished by RacT17N. Thus, these findings suggest a mechanism explaining modified fibrinolysis and tissue remodeling in an oxidized environment... - Developmental changes in the expression of transcription factors GATA-1, -2 and -3 during the onset of human medullary haematopoiesisChristof Dame
Department of Pediatrics, University of Florida, Gainesville 32610 0296, USA
Br J Haematol 119:510-5. 2002....